• BMB Reports
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• 제48권3호
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• pp.159-165
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• 2015

Although the short isoform of ErbB3-binding protein 1 (Ebp1), p42 has been considered to be a potent tumor suppressor in a number of human cancers, whether p42 suppresses tumorigenesis of lung cancer cells has never been clarified. In the current study we investigated the tumor suppressor role of p42 in non-small cell lung cancer cells. Our data suggest that the expression level of p42 is inversely correlated with the cancerous properties of NSCLC cells and that ectopic expression of p42 is sufficient to inhibit cell proliferation, anchorage-independent growth, and invasion as well as tumor growth in vivo. Interestingly, p42 suppresses Akt activation and overexpression of a constitutively active form of Akt restores the tumorigenic activity of A549 cells that is ablated by exogenous p42 expression. Thus, we propose that p42 Ebp1 functions as a potent tumor suppressor of NSCLC through interruption of Akt signaling.

• 성인간호학회지
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• 제23권3호
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• pp.255-266
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• 2011

Purpose: The purpose of this study was to explore nurses' access and use of information resources and to identify the barriers and competency to evidence based practice (EBP). This study used descriptive method to identify baseline data for the purpose of developing strategies for establishing EBP in clinical nursing practice. Methods: Participants in this study were 278 nurses from five hospitals in Daegu and Kyungsangbukdo. The data were collected by self administered questionnaires and SPSS/WIN 15.0 program was used to analyze the data with descriptive statistics, t-test, Pearson's correlation coefficients, and ANOVA. Results: Nurses reported $^*most$ frequently using paper and human resources. The mean score of barriers was $3.02{\pm}0.41$, and competency was $2.70{\pm}0.50$. The reported competency was correlated with nurse factors (r=-.31, p<.001) organization factors (r=-.20, p<.001) and research factors (r=-.12, p<.040) as the barriers to evidence based practice. Conclusion: To promote competency in EBP and to decrease the barriers, it would be necessary to develop the organizational culture that encourages nurses to be involved in research activities. In addition, the development of systemic methods to introduce and establish an education program for facilitating EBP in the clinical settings is needed.

• Mycobiology
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• 제36권1호
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• pp.45-49
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• 2008

Hypoglycemic effects of exo-biopolymers (EBP) produced by submerged mycelial cultures of Coriolus versicolor, Cordyceps sinensis, Paecilomyces japonica, Armillariella mellea, and Fomes fomentarius were investigated in streptozotocin (STZ)-induced diabetic rats. The rats from each experimental group were orally administered with EBPs (100 mg/kg BW) daily for 2 weeks. Though the hypoglycemic effect was achieved in all the cases, however, C. versicolor EBP proved as the most potent one. The administration of the C. versicolor EBP substantially reduced (29.9%) the plasma glucose level as compared to the saline administered group (control). It also reduced the plasma total cholesterol (TC), triglyceride (TG), aspartate aminotransferase (AST) and, alanine aminotransferase (ALT) levels by 9.22, 23.83, 16.93, and 27.31%, respectively. The sugar and amino acid compositions of this EBP were also analyzed in detail.

• 한국통신학회논문지
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• 제19권12호
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• pp.2418-2425
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• 1994

기울기를 따라가는 방식(gradient descent method)에 바탕을 둔 오류 역전파(EBP : Error Back Propagation) 방법이 가장 널리 사용되는 신경회로망의 학습 방법에서 문제가 되는 지역 최소값(local minima), 느린 학습 시간, 신경망 구조(structure), 그리고 초기의 연결 강도(interconnection weight) 등을 기존의 다층 신경 회로망에 지역적인 학습 능력을 가진 가우시안 포텔샵 네트워크(GPFN : Gaussian Potential Function Networks)를 병렬적으로 부가하여 해결함으로써 지역화된 오류 학습 패턴들이 나타내는 문제에 대하여 학습 성능을 향상시킬 수 잇는 새로운 학습 방법을 제시한다. 함수 근사화 문제에서 기존의 EBP 학습 방법과의 비교 실험으로 제안된 학습 방법이 보다 개선된 일반화 능력과 빠른 학습 속도를 가짐을 보여 그 효율성을 입증한다.

• 대한전기학회논문지
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• 제40권2호
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• pp.224-233
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• 1991

The multilayer expanson of single layer NN (Neural Network) was needed to solve the linear seperability problem as shown by the classic example using the XOR function. The EBP (Error Back Propagation ) learning rule is often used in multilayer Neural Networks, but it is not without its faults: 1)D.Rimmelhart expanded the Delta Rule but there is a problem in obtaining Ca from the linear combination of the Weight matrix N between the hidden layer and the output layer and H, wich is the result of another linear combination between the input pattern and the Weight matrix M between the input layer and the hidden layer. 2) Even if using the difference between Ca and Da to adjust the values of the Weight matrix N between the hidden layer and the output layer may be valid is correct, but using the same value to adjust the Weight matrixd M between the input layer and the hidden layer is wrong. Recognitron III was proposed to solve these faults. According to simulation results, since Recognitron III does not learn the three layer NN itself, but divides it into several single layer NNs and learns these with learning patterns, the learning time is 32.5 to 72.2 time faster than EBP NN one. The number of patterns learned in a EBP NN with n input and output cells and n+1 hidden cells are 2**n, but n in Recognitron III of the same size. [5] In the case of pattern generalization, however, EBP NN is less than Recognitron III.

• Biomolecules & Therapeutics
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• 제18권2호
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• pp.197-204
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• 2010

To establish the anti-inflammatory activity of the total flavonoid fraction of the root barks of Broussonetia papyrifera (EBP) and a new formula, the ethanol extract of the root barks of B. papyrifera was fractionated with ethylacetate, yielding the hydrophobic prenylated flavonoid-enriched fraction. EBP and the ethanol extract of the whole Lonicera japonica (ELJ) plant were then mixed at a ratio of 1:1 (w/w) to give a new preparation (BL) in the hope of obtaining an optimal formula with a higher anti-inflammatory activity. Evaluation of the effects of these preparations on A23187-treated rat basophilic leukemia (RBL-1) cells revealed that EBP potently inhibited 5-lipoxygenase (5-LOX), while ELJ showed weak inhibition. Additionally, the mixture (BL) clearly showed stronger inhibitory effects against 5-LOX than either preparation alone. These preparations also inhibited cyclooxygenase-2-catalyzed $PGE_2$ and inducible nitric oxide (NO) synthase-catalyzed NO production by lipopolysaccharide-treated RAW 264.7 cells. When tested against arachidonic acid-induced mouse ear edema, EBP showed strong inhibitory activity at doses of 5-200 mg/kg when administered orally, but BL had obviously stronger inhibitory effects. When tested against ${\lambda}$-carrageenan-induced paw edema in mice, BL showed a potent and synergistic anti-inflammatory effect. In addition, in the acetic acid-induced writhing test, BL was found to have strong analgesic activity at 50-400 mg/kg. Taken together, these results indicate that each of these preparations exert anti-inflammatory activity in vitro and in vivo. In particular, BL showed stronger anti-inflammatory activity than EBP, and these anti-inflammatory effects were partially related to the inhibition of eicosanoid and NO production. BL may be useful for the treatment of human inflammatory disorders.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.359-365
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• 2013

The high mobility group box 1 (HMGB1) protein is a multifunctional cytokine-like molecule that plays an important role in the pathogenesis of tumors. In this study, real-time polymerase chain reactions and Western blot assays indicated that HMGB1 transcriptional activity and protein level are increased in $Tax^+$-T cells (TaxP). To clarify the mechanisms, a series of HMGB1 deletion reporter plasmids (pHLuc1 to pHLuc6) were transfected into $Tax^-$-T cells (TaxN, Jurkat) and $Tax^+$-T cells (TaxP). We found that promoter activity in $Tax^+$-T cells to be higher than that in $Tax^-$-T cells, indicating a significant increase in pHLuc6. Bay11-7082 (NF-${\kappa}B$ inhibitor) treatment did not block the enhancing effect. Chromatin immunoprecipitation assays revealed that Tax was retained on a HMGB1 promoter fragment encompassing -1163 to -975. Bioinformatics analysis showed six characteristic cis-elements for CdxA, AP-1, AML-1a, USF, v-Myb, and C/EBP in the fragment in question. Mutation of cis-elements for C/EBP reduced significant HMGB1 promoter activity induced by Tax. These findings indicate that Tax enhances the expression of HMGB1 gene at the transcriptional level, possibly by interacting with C/EBP.

• 대한화장품학회지
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• 제35권2호
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• pp.111-116
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• 2009

한국에서 '가가이모과잎'이라고 불리는 인도의 전통 식물 약재인 Gymnema sylvestre는 당뇨의 원인인 생체 내당의 대사를 조절하는 약재로 이용되어 왔다. 본 연구에서는 Gymnema sylvestre 잎으로부터 인공적으로 조직 배양한 캘러스를 대량 배양하였다. 배양된 캘러스부터 추출물을 얻은 후 지방 전구 세포인 3T3-Ll 세포를 이용하여 항비만 효과를 관찰하였다. 결과적으로, Gymnema sylvestre 잎으로부터 조직 배양한 캘러스 추출물은 지방 전구 세포의 지방세포로의 분화뿐만 아니라 세포 내 지질 축적을 억제 하였다. 또한 지방세포의 형성을 조절하는 초기 지방세포 내의 전사인자인 C/EBP-${\alpha}$ 발현을 억제하면서 인슐린에 의해 유도된 지방세포의 분화를 억제하였다. 따라서 이러한 결과들은 피부를 위한 화장품 성분으로서 응용 가능성을 제공할 수 있을 것으로 사료된다.

• 한국도서관정보학회지
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• 제50권1호
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• pp.43-60
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• 2019

본 연구의 목적은 대학도서관 사서가 인식한 근거기반실무의 지식 정도와 영향 요인을 파악하여 대학도서관의 경영과 사서직 발전에 기초자료를 제공하는데 있다. 본 연구에 응답한 대학도서관 사서의 근거기반실무 지식에서 유의한 차이가 나타난 변인은 연령, 교육정도, 고용유형, 직위, 학술지읽기, 학술대회 참여이었다. 이들의 근거기반실무 지식에 영향을 미치는 예측 요인은 교육정도, 학술지읽기로 나타났다. 또한, 근거기반실무 지식 중 근거의 비판적 평가와 실무적용 및 연구문헌 이해와 연구수행은 취약한 것으로 밝혀졌다. 대학도서관 사서의 근거기반실무 지식은 대학도서관과 사서직의 지속적 발전을 위한 혁신의 촉진요인이라고 간주할 수 있기 때문에 근거기반실무의 지식역량을 향상시킬 수 있도록 사서 개인의 노력 뿐 아니라 연구 관련 활동을 지원하는 조직단위, 도서관계, 학계차원의 지원과 정책이 필요하다.

• The Korean Journal of Pain
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• 제25권2호
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• pp.112-115
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• 2012

Intracranial hypotension is characterized by a postural headache which is relieved in a supine position and worsened in a sitting or standing position. Although less commonly reported than postural headache, sixth nerve palsy has also been observed in intracranial hypotension. The epidural blood patch (EBP) has been performed for postdural puncture headache, but little is known about the proper timing of EBP in the treatment of sixth nerve palsy due to intracranial hypotension. This article reports a case of sixth nerve palsy due to spontaneous intracranial hypotension which was treated by EBP 10 days after the onset of palsy.