• BMB Reports
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• 제50권3호
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• pp.126-131
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• 2017

P48 Ebp1 is expressed in rapidly proliferating cells such as cancer cells and accelerates cell growth and survival. However, its expression pattern and role in central nervous system development have not been studied. Here, we demonstrated the spatiotemporal expression pattern of p48 Ebp1 during embryonic development and the postnatal period. During embryonic development, p48 Ebp1 was highly expressed in the brain. Expression gradually decreased after birth but was still more abundant than p42 expression after birth. Strikingly, we found that p48 Ebp1 was expressed in a cell type specific manner in neurons but not astrocytes. Moreover, p48 Ebp1 physically interacted with beta tubulin but not alpha tubulin. This fits with its accumulation in distal microtubule growth cone regions. Furthermore, in injured hippocampal slices, p48 Ebp1 introduction promoted axon regeneration. Thus, we speculate that p48 Ebp1 might contribute to microtubule dynamics acting as an MAP and promotes CNS axon regeneration.

• 한국간호교육학회지
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• 제17권3호
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• pp.346-354
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• 2011

Purpose: This study examines the effects of integrating Evidence Based Practice (EBP) into a critical care clinical practicum on nursing students' access and use of information resources and EBP competency. Methods: A one-group pretest-posttest design was used. Fifty senior nursing school students from a university participated. A critical care clinical practicum combined with EBP consisted of six full days of clinical practicum in intensive care units with EBP education. Group and individual training in EBP skills, lectures, small group discussion and conferences were provided. Data were analyzed using paired t tests for 50 participants. Results: The scores of evidence based practice competency increased significantly (p<.001) showing significant improvement in searching and classifying the evidence. Nursing students' access and use of research evidence improved (p=.004). Conclusion: This study showed that the integration of EBP into a clinical practicum was effective in improving accessibility and usefulness in research evidence such as guidelines and research articles, and increasing EBP competency in undergraduate students.

• 간호행정학회지
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• 제18권2호
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• pp.202-212
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• 2012

Purpose: As Evidence-Based Practice (EBP) has increasingly been proven as a means of cost-effective and higher-quality healthcare, its successful implementing are challenging. This study done to identify EBP beliefs, knowledge and performance among nurses experienced as a preceptor. Method: A descriptive cross-sectional survey was conducted with a convenience sample of 249 preceptor nurses working in 9 general hospitals in Korea. Reliable and valid questionnaires (EBP beliefs scale, Evidence Based Practice Questionnaire, Research-related activities) were used and the data were analyzed using SPSS win 17.0. Result: Perceived beliefs on EBP were relatively positive (mean score 3.57 out of 5), and the level of knowledge was moderate (4.21 out of 7). However, performance of EBP was low (3.82 out of 7). Regularity in reading research journals and searching evidences using core web-database were rarely conducted. Statistically significant correlations were found between beliefs, knowledge and performance of EBP (all p<.05). Conclusions: This result indicates that education and training programs to facilitate EBP performance are needed among preceptor nurses.

• 한국지능시스템학회논문지
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• 제9권5호
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• pp.490-495
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• 1999

본 논문에서는 보로노이 다이아그램을 이용하여 오류 역전파 신경망의 초기값을 결정할수 있는 VoD_EBP를 제안하였다. VoD_EBP는 초기 연결 가중치와 임계값을 공학적 계산방법으로 결정함으로써 기존의 EBP에서 자주 발생하는 학습 마비 현상을 피할수 있고 초기부터 빠른 속도로 학습이 진행되므로 학습횟수를 단축시킬수 있다, 또한 VoD_EBP는 은닉층의 노드 수를 보로노이 다각형으로 구분된 클러스터들의 개수로 정할 수있어 신경망 설계에 신뢰성을 향상시켰다. 제시된 VoD_EBP의 효율성을 입증하기 위해 간단한 실험으로 2차원 입력벡터를 갖는 XOR 문제와 3차원 패리티 코드 검출 문제에 대하여 적용하여 보았다. 그 결과 임의의 초기값으로 설정하였던 EBP보다 훨씬 빠르게 학습이 종료되었고, 지역 최소치에 빠져 학습이 진행되지 못하는 현상이 발생하지 않았다.

• BMB Reports
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• 제54권8호
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• pp.413-418
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• 2021

ErbB3-binding protein 1 (EBP1) is a multifunctional protein associated with neural development. Loss of Ebp1 leads to upregulation of the gene silencing unit suppressor of variegation 3-9 homolog 1 (Suv39H1)/DNA (cytosine 5)-methyltransferase (DNMT1). EBP1 directly binds to the promoter region of DNMT1, repressing DNA methylation, and hence, promoting neural development. In the current study, we showed that EBP1 suppresses histone methyltransferase activity of Suv39H1 by promoting ubiquitin-proteasome system (UPS)-dependent degradation of Suv39H1. In addition, we showed that EBP1 directly interacts with Suv39H1, and this interaction is required for recruiting the E3 ligase MDM2 for Suv39H1 degradation. Thus, our findings suggest that EBP1 regulates UPS-dependent degradation of Suv39H1 to govern proper heterochromatin assembly during neural development.

• Journal of Animal Science and Technology
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• 제46권6호
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• pp.909-916
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• 2004

CCAAT/enhancer binding protein(C/EBP)는 지방전구세포의 분화과정에서 말현하는 전사인자군에 속한다. 이러한 C/EBP 중에서 C/EBP $\alpha$ 는 지방세포의 분화와 지방침착에 있어 중요한 역할을 수행허는 것으로 알려져 있다. 본 연구에서는 한우 C/EBP$\alpha$ 유전자를 분리 동정하고 그 발현을 조사하였다. 한우 C/EBP$\alpha$ 유전자는 1059bp의 open reading frame으로 구성되어 있으며 353개의 아미노산을 암호화하고 있었다. 그리고 한우 C/EBP $\alpha$ 아미노산을 다른 종과 비교하였을 때 매우 높은 상동성을 나타내었다. Northern blotting 분석에 의하여 12개월 한우에 있어서 C/EBP$\alpha$ mRNA의 분석을 실시한 결과 지방조직에서 가장 높은 말현을 나타내었으며 대장과 폐에서 매우 낮은 말현을 확인할수 있었다. 또한 12개월26개월 30개월 한우 의 등심과 지방에 있어서 C/EBP$\alpha$ 유전자의 발현을 real-time RT-PCR로 분석한 결과 등심 에서는 26개월에 서 가장 높은 발현을 보였으며 지방에서는 12개월과 26개월에 서 높은 발현을 나타내었다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1989년도 하계종합학술대회 논문집
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• pp.457-460
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• 1989

In this paper, an improvement of the EBP(error back propagation) learning speed using an acceleration algorithm is described. Using an acceleration algorithm known as the Partan method in the gradient search algorithm, learning speed is 25% faster than the original EBP algorithm in the simulaion results.

• 한국콘텐츠학회논문지
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• 제18권6호
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• pp.38-49
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• 2018

근거기반실무 간호교육은 학습자의 수준에 따라 단계적으로 경험하며 숙련되도록 이끌어야한다. 본 연구는 근거기반실무의 일부를 통합한 간호학과 2학년의 기본간호학 수업을 운영하고 근거기반실무의 기초역량 향상에 대한 효과를 검증하였다. 연구 설계는 비동등성 대조군 전후 유사실험설계이었다. 대상자는 2개 대학교의 간호학과 2학년 재학생 108명이었다. 실험군은 2016년 2학기 15주 수업기간 중 총 8주, 24시간의 근거기반실무를 활용한 기본간호학 수업에 참여하였고 대조군은 일반적 기본간호학 수업에 참여하였다. 자료는 자가보고형의 문제해결 적극성, 근거기반실무 적용의도, 그리고 비판적 사고성향 측정도구로 수집하였다. 수집된 자료는 SPSS/WIN 23.0 program을 이용하여 동질성은 Chi-square 검정과 독립표본 t-검정으로, 가설 검정은 독립표본 t-검정으로 분석하였다. 분석 결과 실험군은 대조군에 비해 문제해결 적극성과 근거기반실무 적용의도에서 유의한 증가를 보였다. 그러나 비판적 사고는 두 군 간에 차이가 없었다. 근거기반실무를 활용한 기본간호학 수업은 저학년 학생의 근거기반실무 기초역량인 문제해결 적극성과 근거기반실무 적용의도의 증가에 효과적이었다. 근거기반실무에 대한 학년별 목표에 적합한 교과과정에 의한 간호학생들의 근거기반실무 역량과 비판적 사고의 향상에 대한 장기적 노력이 더 필요하다.

• 셀메드
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• 제6권3호
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• pp.15.1-15.4
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• 2016

Evidence based practice (EBP) is a system of applying the most current and valid high quality evidence to support clinical decision making in a healthcare setting. In the twenty five years since its inception, EBP has become the accepted benchmark for excellence in healthcare. Although the system emerged within the biomedical sciences, in the years since EBP has become normative across all healthcare modalities from dentistry, allied health to complementary and alternative medicine (CAM). Practicing evidence based medicine within any modality potentially offers the patient the best available care based on high quality evidence. Yet it is the nature of the evidence that provokes some questions about the suitability of EBP across all modalities of healthcare. The meta analysis of randomized controlled trial (RCT) stands at the pinnacle of the hierarchy of evidence in EBP. This forms a challenge to CAM due to the difficulty in reducing the elementals of a holistic naturopathic assessment of a patient into an answerable question to be tested within a RCT. On one level this makes EBP paradigmatically incompatible with CAM, yet on another level it presents the opportunity to redefine the parameters of what is considered high level evidence. EBP has become a tool, and at times a weapon wielded by governments and health insurance companies to direct healthcare funding and policy. The implications of the nature of accepted evidence are becoming far reaching. The pursuit of the best available healthcare for each individual is the focus of EBP. However, the injudicious use of this system to direct health policy is fraught with biomedical bias and dominance. This issue raises the challenge to CAM to present high level evidence according to the rules of evidence, or face the annihilation of centuries of empirical knowledge.

• BMB Reports
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• 제52권6호
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• pp.391-396
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• 2019

Receptor activator of nuclear factor kappa B ligand (RANKL) expression in osteoblasts is regulated by 1,25-dihydroxyvitamin D3 (1,25D3). CCAAT/enhancer-binding protein beta ($C/EBP{\beta}$) has been proposed to function as a transcription factor and upregulate RANKL expression, but it is still uncertain how $C/EBP{\beta}$ is involved in 1,25D3-induced RANKL expression of osteoblasts. 1,25D3 stimulation increased the expression of RANKL and $C/EBP{\beta}$ genes in osteoblasts and enhanced phosphorylation and stability of these proteins. Moreover, induction of RANKL expression by 1,25D3 in osteoblasts was downregulated upon knockdown of $C/EBP{\beta}$. In contrast, $C/EBP{\beta}$ overexpression directly upregulated RANKL promoter activity and exhibited a synergistic effect on 1,25D3-induced RANKL expression. In particular, 1,25D3 treatment of osteoblasts increased $C/EBP{\beta}$ protein binding to the RANKL promoter. In conclusion, $C/EBP{\beta}$ is required for induction of RANKL by 1,25D3.