• 대한본초학회지
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• 제25권3호
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• pp.65-71
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• 2010

Objectives：To develop and validate HPLC-PDA methods for simultaneous determination of seven constituents in Samchulkunbi-tang (SKT). Additionally, we investigated the cytotoxicity against BEAS-2B cell line and splenocytes of SKT. Methods：Reverse-phase chromatography using a Gemini $C_{18}$ column operating at $40^{\circ}C$, and photodiode array (PDA) detection at 230, 254 and 280 nm, were used for quantification of the three marker components of SKT. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. The cytotoxicity of SKT were measured by the CCK-8 assay method. Results：Calibration curves were acquired with $r^2$>0.9999, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 6.0%. The recovery rate of each compound was in the range of 86.89-109.78%, with an RSD less than 4.0%. The contents of seven compounds in SKT were 1.39-6.84 mg/g. SKT had no cytotoxicity effect at 50-200 ${\mu}g$/mL concentrations. Conclusions：The established method will be helpful to improve quality control and in vitro efficacy study of SKT.

• 대한한의학회지
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• 제32권3호
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• pp.25-34
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• 2011

Objectives: We performed simultaneous determination of five constituents by HPLC in Samul-tang (SMT). Additionally, we investigated the immune-stimulatory potential of SMT on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice. Methods: Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array (PDA) detection at 190-400 nm, were used for quantification of the five components of SMT. Mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. C57BL/6 mice were immunized intraperitoneally with OVA/alum ($100{\mu}g/200{\mu}g$) on days 1, 8, and 15. The extract of SMT (1000 mg/kg) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Results: Calibration curves were acquired with $r^2$>0.9999, and the relative standard deviation (RSD, %) for intra- and inter-day precision were both less than 3.5%. The recovery was in the range of 95.69-115.12%, with an RSD less than 6.0%. The contents of five components in SMT were 1.08-15.30 mg/g. SMT significantly enhanced Con A-induced splenocyte proliferation in OVA-immunized mice (p<0.01). Also, SMT significantly enhanced OVAspecific IgG, IgG1 and total IgM levels in plasma compared with the OVA-immunized group. Conclusions: The established method will be applied for the quantification of major components and immunestimulating activity in OVA-immunized mouse model of SMT.

• 대한한의학회지
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• 제32권3호
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• pp.10-17
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• 2011

Objective: To evaluate the immune-stimulatory potential of extracts of Broussonetia kazinoki Siebold (BK) on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice. Material and Methods: C57BL/6 mice were immunized intraperitoneally with OVA/alum ($100{\mu}g/200{\mu}g$) on days 1, 8, and 15. BK (100, 300 or 1000 mg/kg) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Further, the effects of BK on expression of cytokine mRNA in OVA-immunized mice splenocytes were evaluated by RT-PCR analysis. Results: BK significantly enhanced OVA-, LPS-, and Con A-induced splenocyte proliferation in OVA-immunized mice (p<0.01). BK also significantly enhanced total IgM and OVA-specific IgG1 levels in plasma compared with the OVA control group. Moreover, BK up-regulated significantly the expression of mRNA level of IL-2 and IFN-${\gamma}$ in splenocytes. Conclusions: BK has immune-stimulating activity in an OVA-immunized mouse model system, enhancing the Th1 immune response. BK showed no cytotoxicity in this system, suggesting that BK may be a safe and effective adjuvant in humans.

• 동의생리병리학회지
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• 제24권3호
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• pp.426-429
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• 2010

This study was conducted to investigate the acute toxicity and safety of Pyungwi-san (Pingwei-san) in Sprague-Dawley rat though the current regulatory guideline. The preliminary study showed that the single oral administration of Pyungwi-san (Pingwei-san) did not induce any toxic effect at a dose level of 2000 mg/kg. Based on the results, 2000 mg/kg was selected as the limited dose. In this study, 10 rats of each sex were randomly assigned to two groups of 5 rats each and were administrated singly by gavage at dose levels of 0 and 2000 mg/kg. After single administration, Mortalities, clinical signs, body weight changes, gross findings were observed for the 15-day period. Throughout the study period, no treatment-related deaths were observed. There were no adverse effects on clinical signs, body weight, and gross findings at all treatment groups. These results showed that the single oral adminstration of Pyungwi-san (Pingwei-san) did not cause any toxic effect at the dose levels of 2000 mg/kg in rats. In conclusion, the $LD_{50}$ of Pyungwi-san (Pingwei-san) was considered to be over 2000 mg/kg body for both sexes.

• 대한본초학회지
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• 제26권4호
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• pp.187-194
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• 2011

Objective : Astragali Radix (AR) and Cinnamomi Cortex (CC) are used to enhance immune response in Asian traditional medicine. Immuno-potentiation of the combination of AR and CC were evaluated on the cellular and humoral immune response using murine macrophage cell line (RAW 264.7) and OVA-immunized mice. Methods : This study was designed to investigate the immuno-potentiative effects of AR, CC, and AR with CC on nitric oxide synthesis in RAW 264.7 cells and proliferation and production levels of Intereukin-2 (IL-2) in mouse splenocytes. In addition, we evaluated the plasma-specific antibody responses and splenocyte proliferation on ovalbumin (OVA)-immunized mice treated with herbal extracts. Results : Combination treatment with AR and CC increased nitric oxide synthesis in RAW 264.7 cells and IL-2 level in splenocytes (p<0.001). Combination of AR and CC significantly enhanced the Concanavalin A- (Con A ; T cell mitogen) and lipopolysaccharide-(LPS ; B cell mitogen) induced splenocyte proliferation on the OVA-immunized mice. Combination of AR and CC also significantly enhanced plasma levels of OVA-specific IgG (p<0.01), IgG1 (p<0.05) and total IgM (p<0.01) compared with the OVA-immunized control group. Conclusion : These results suggest that combination of AR and CC could be used as therapeutic profile on activation of immune response.

• 한국정보통신학회논문지
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• 제26권5호
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• pp.641-648
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• 2022

본 논문은 3D 프린터 출력 방식 중 하나인, 전자빔용해법(EBM)의 공정 간에 발생하는 결함에 영향을 미치는 요인들을 데이터 분석을 통해 규명하는 연구이다. 선행 연구들을 기반으로 결함발생에 주요한 원인으로 지목되는 요소들을 참고하였으며, 공정 간 발생하는 로그파일 분석을 통해 결함 발생과 연관된 변수들을 추출하였다. 또한, 해당 데이터가 시계열 데이터라는 점에 착안하여 window의 개념을 도입하여, 현재 공정 층으로부터 총 3개 전 층까지의 데이터를 포함하여 분석에 사용 될 변수들을 구성하였다. 해당 연구의 종속변수는 결함발생유무이기에 이진분류를 통한 분석을 하였으며, 이때 결함 층의 비율이 낮다는(약 4%) 문제로 인해 SMOTE 기법을 적용하여 균형잡힌 훈련용 데이터를 만들었다. 분석을 위해 Gridsearch CV를 활용한 XGBoost를 사용하였고, 분류 성능은 혼동행렬을 기반으로 평가하였다. 마지막으로, SHAP값을 통한 변수 중요도 분석을 통해 연구의 결론을 내렸다.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2005년도 추계 학술대회논문집
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• pp.105-110
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• 2005

Evaluation of elliptic blending turbulence model (EBM) together with the two-layer model, shear stress transport (SST) model and elliptic relaxation model (V2-F) is performed for a better prediction of thermal stratification in an upper plenum of a liquid metal reactor by applying them to the experiment conducted at JNC. The algebraic flux model is used for treating the turbulent heat flux. There exist much differences between turbulence models in predicting the temporal variation of temperature. The V2-F model and the EBM better predict the steep gradient of temperature at the interface of thermal stratification, and the V2-F model and EBM predict properly the oscillation of temperature. The two-layer model and SST model fail to predict the temporal oscillation of temperature.

• Natural Product Sciences
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• 제16권3호
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• pp.180-184
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• 2010

The simple and accurate method was established for the simultaneous determination of (-)-menthone and (-)-menthol in Menthae herba obtained from Korea and China. A quantitative analysis was performed with a gas chromatography-flame ionization detector and reference compounds were separated on a capillary HP-Innowax column (30 m $\times$ 0.23 mm, 0.50 ${\mu}m$, Agilent, MA, USA). The correlation coefficients of the compounds showed good linearity ($r^2$ > 0.9997) over the linear range. The precision, repeatability and stability showed less than 1.7% of relative standard deviation (RSD) values for two compounds. Recovery rates were within the range of 95.72 - 103.76%. The method was applied successfully to analyze 15 samples of Menthae herba and achieved sufficient and specific separation of reference compounds. The principal component analysis (PCA) exhibited the classification of 15 samples according to their locations of origin.

• 보험의학회지
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• 제32권1호
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• pp.8-14
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• 2013

Evidence-based medicine(EBM) or evidence-based health care has been used in clinical medicine. Recently, it also has been applied to areas of social medicine such as independent medical examination. Insurance medicine is one of social medicine, as for it's role is maintenance of sound insurance system or for insured of life insurance. Practical application of EBM to the insurance medicine are; confirming question, finding evidence, and evaluation that selected evidence is best or not in terms of practical aspect.

• 한방비만학회지
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• 제7권2호
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• pp.61-75
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• 2007

Objectives : The purpose of this study was to investigate correlation between obese degree, result of hair tissue mineral analysis and bony maturation. And we also wanted to compare the differences between early bony maturation group(EBM) and late bony maturation group(LBM). Methods : 146 subjects who visited growth clinic were measured by BMI, PBF(percent body fat), percent BMI, bone age esimation and HTMA(hair tissue mineral analysis). The patients were classified into two groups - EBM, LBM group - according to the gap of bone age and chronological age. It was analysed that the correlation of bony maturation and obese degree, nutritional elements, heavy metals, significant ratio of nutritional elements. Also, analysed the differences between groups. Results : 1. BMI, percent BMI had a correlation with bony maturation, PBF however didn't have a correlation with it. 2. Ca, Mg, Zn and P had a positive correlation with bony maturation, and also K, Cr and Mo had a negative correlation. 3. U, As and Cd had a negative correlation with bony maturation. 4. Ca/P, Na/K, Ca/K and Zn/Cu had a positive correlation with bony maturation, and also Na/Mg, Ca/Mg had a negative correlation. 5. Percent BMI, Ca, Mg, Mn and Ca/p were higher in EBM group. 6. K, Mo and Hg were lower in EBM group. Conclusion : According to this study it could be suggested that maintaining proper percent BMI and accumulation of nutritional elements, heavy metals to prevent early bony maturation.