• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.9A
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• pp.755-761
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• 2005

In this paper, we proposed a channel estimation method by impulse signal train in OFDM. In order to estimate the channel response, 4 impulse signals are generated and transmitted during one OFDM (Orthogonal Frequency Division Multiplexing) symbol. The intervals between the impulse signals are all equal in time domain. At the receiver, the impulse response signals are summed and averaged. And then, the averaged impulse response signal is zero padded and fast Fourier transformed to obtain the channel estimation. The BER performance of the proposed method is compared with those of conventional estimation method using the long training sequence in fast fading environments. The simulation results show that the proposed method improves by 3 dB in terms of Eb/No, compared with the conventional method.

• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3695-3702
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• 2013

A nickel(II) complex $[Ni(H_2biim)_2(H_2O)_2](ClO_4)_2{\cdot}H_2O$ (1) of biimidazole ligand has been synthesized and characterized (Where $H_2biim$ = 2,2'-biimidazole). The single crystal X-ray diffraction of the complex shows a dimeric structure with six coordinated psudo-octahedral geometry. The cyclic voltammograms of complex exhibited one quasireversible reduction wave ($E_{pc}=-0.61V$) and an irreversible oxidation wave ($E_{pa}=1.28V$) in DMF solution. The interaction of the complex with Calf-Thymus DNA (CT-DNA) has been investigated by absorption and fluorescence spectroscopy. The complex is an avid DNA binder with a binding constant value of $1.03{\times}10^5M^{-1}$. The results suggest that the nickel(II) complex bind to CT-DNA via intercalative mode and can quench the fluorescence intensity of EB bind to CT-DNA with $K_{app}$ value of $3.2{\times}10^5M^{-1}$. The complex also shown efficient oxidative cleavage of supercoiled pBR322 DNA in the presence of hydrogen peroxide as oxidizing agent. The DNA cleavage by complex in presence of quenchers; viz. DMSO, KI, $NaN_3$ and EDTA reveals that hydroxyl radical or singlet oxygen mechanism is involved. The complex showed invitro antimicrobial activity against four bacteria and two fungi. The antimicrobial activity was nearer to that of standard drugs and greater than that of the free ligand.

• Journal of the Korean GEO-environmental Society
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• v.10 no.5
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• pp.33-39
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• 2009

This study was conducted to evaluate production of complex odor and 12 specific odorous compounds in a pilot-scale (capacity : $100m^3$) ultra thermophilic aerobic composting. There were three types input: municipal wasted sludge, livestock manure and slurry, and food waste produced in Jung-Eb city. Each raw material was mixed with seed material and operated for two periods (1st : 50 days, 2nd : 60days). During composting, the temperature hit $90{\sim}95^{\circ}C$ after every mixing in both periods. Therefore, it was concluded that increasing temperature also saves the time which required for composting and high reduction of organics and water contents. The primary odorous compounds were ammonia, methyl mercaltan, dimethyl disulfide and trimethylamine. The concentration of the primary compounds and complex odor during the operation were higher than those on final day and most compounds did not exceed the allowable exhaust standard for odor. Also, it was found that optimal mixing time and control of high temperature are the most important parameters for odor control in ultra thermophilic aerobic composting.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.12
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• pp.5189-5193
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• 2016

Objective: Dorema glabrum Fisch. & C.A. Mey is a perennial plant that has several curative properties. Anti-proliferative activity of seeds of this plant has been demonstrated in a mouse fibrosarcoma cell line. The aim of the present study was to evaluate cytotoxicity of D. glabrum root extracts in a human gastric adenocarcinoma (AGS) cell line and explore mechanisms of apoptosis induction, cell cycle arrest and altered gene expression in cancer cells. Materials and Methods: The MTT assay was used to evaluate IC50 values, EB/AO staining to analyze the mode of cell death, and flow cytometry to assess the cell cycle. Quantitative real-time polymerase chain reaction (qRT-PCR) amplification was performed with apoptosis and cell cycle-related gene primers, for cyclin D1, c-myc, survivin, VEGF, Bcl-2, Bax, and caspase-3 to determine alteration of gene expression. Results: Our results showed that n-hexane and chloroform extracts had greatest toxic effects on gastric cancer cells with IC50 values of $6.4{\mu}g/ml$ and $4.6{\mu}g/ml$, respectively, after 72 h. Cell cycle analysis revealed that the population of treated cells in the G1 phase was increased in comparison to controls. Cellular morphological changes indicated induction of apoptosis. In addition, mRNA expression levels of Bax and caspase-3 were increased, and of bcl-2 survivin, VEGF, c-myc and cyclin D1 were decreased. Conclusion: Our study results suggest that D. glabrum has cytotoxic effects on AGS cells, characterized by enhanced apoptosis, reduced cell viability and arrest of cell cycling.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.465-465
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• 2014

증식블랑켓모듈(TBM, Test Blanket Module)을 개발하여 왔다. 이 두 증식블랑켓모듈은 모두 헬륨냉각을 기반으로 개발 되어왔으며 이에 따라, 헬륨순환기, 헬륨히터 및 헬륨열교환기 등에 대한 기본적인 연구가 수행되었다. 이후 2012년 고체형 증식블랑켓모듈을 ITER TBM 개념으로 주도하기로 결정함에 따라, HCCR (Helium Cooled Ceramic Reflector) TBM의 보조계통인 하나인 헬륨냉각계통(HCS, Helium Cooling System)에 대한 개발이 본격적으로 이루어졌다. 한국원자력연구원에서는 HCCR TBM의 냉각성능을 만족하기 위하여 8 MPa, 1.5 kg/s 및 $300/500^{\circ}C$ (입구/출구 온도)의 운전조건을 갖는 헬륨냉각계통의 설계를 완료하였다. 설계된 헬륨냉각계통은 HCCR TBM에서 회수된 약 $450^{\circ}C$의 헬륨을 열회수기(recuperator)기와 냉각기를 통해 상온으로 냉각시킨 후, 필터를 통해 헬륨을 여과시킨다. 여과된 헬륨은 헬륨순환기에 의해 가압되어 열회수기를 다시 지나 $300^{\circ}C$ 이상으로 가열된다. 가열된 헬륨은 열회수기를 지나지 않는 상온의 헬륨과 혼합되어 최종적으로 HCCR TBM의 입구온도 조건인 $300^{\circ}C$로 맞추어 HCCR TBM에 공급된다. 이러한 열회수기 중심으로 '${\infty}$' 모양의 자가 교차로 설계된 헬륨냉각계통은 고온영역과 저온영역으로 냉각회로를 구분하여 순환기, 필터 및 각종 계측기의 운전온도 환경을 상온으로 유지시킬 수 있어 운전 및 유지보수 관점에서 이점이 있다. HCCR TBM의 헬륨냉각계통 설계 및 핵심 기기를 실증하고, 운전 경험을 쌓기 위하여 헬륨공급장치(HeSS, Helium Supply System)를 헬륨유량기준 1/3 규모(0.5 kg/s)로 구축하였으며, '14년까지 HeSS를 실증규모로 업그레이드 하기 위하여 80기압 환경에서 압축비 1.1, 유량 1.5 kg/s의 성능을 내는 헬륨순환기를 설치할 예정이다. 현재 구축된 1/3 규모 HeSS는 국내 구축된 전자빔 고열부하 시험 장비인 KoHLT-EB (Electron Beam)와 연계되어 HCCR TBM의 일차벽(플라즈마 대향부품)을 검증할 예정이며, 이를 통해 얻어진 열수력 DB는 현재 개발중인 핵융합로 안전해석코드인 GAMMA-FR 검증에 활용될 계획이다.

• Korean Journal of Applied Biomechanics
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• v.19 no.1
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• pp.59-66
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• 2009

The purpose of this study was to evaluate flatness of swing plane and determine swing plane type using 3-D swing plane analysis from young elite male golf players. This study also investigate the possibility of determination of swing plane using other kinematic parameters except flatness. As results, no correlations was found between flatness and handicap. Comparison of flatness between single plane and multiple plane swing group were performed and found a significant difference. The error range of flatness, 10cm, which was used for distinguish swing plane type was effective since significant differences were found at MB, EB, and EF. These differences were typical characteristics to classify two swing styles. Other kinematic parameters such as unit vector components of shaft and displacement of shaft end point also compared per event but found no significant differences. However, the moving patterns of these parameters during a golf swing showed such characteristics of each swing plane type well that these parameters could be used to determine swing style as an indirect barometers.

• Journal of Korean Tunnelling and Underground Space Association
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• v.19 no.5
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• pp.717-732
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• 2017

Recently, the construction of underground structure such as a double-deck tunnel is increasing to manage rapid growth of roadway traffic volume. Double-deck tunnel includes middle slab to separate upper and lower road inside, and various sources affect the dynamic behaviour of middle slab due to dynamic loading of vehicle. Therefore, it is important to investigate the dynamic response of middle slab precisely to apply it to design and analysis of double-deck tunnel. In this study, dynamic analysis model of middle slab considering structural type, design velocity, vehicle load, and surface roughness, etc. is built. 3-dimensional dynamic analysis is performed to assess dynamic response of middle slab. Consequently, Dynamic Magnification Factor which represents dynamic response of middle slab shows maximum in case of elastomeric bearings (EB) and average roughness (Grade C). It is also expected that dynamic response can be reduced under the condition of good roughness (Grade B) and fixed bearings (FB).

• Development and Reproduction
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• v.21 no.4
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• pp.425-434
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• 2017

Polyploidy is occurred by the process of endomitosis or cell fusion and usually represent terminally differentiated stage. Their effects on the developmental process were mainly investigated in the amphibian and fishes, and only observed in some rodents as mammalian model. Recently, we have established tetraploidy somatic cell nuclear transfer-derived human embryonic stem cells (SCNT-hESCs) and examined whether it could be available as a research model for the polyploidy cells existed in the human tissues. Two tetraploid hESC lines were artificially acquired by reintroduction of remained 1st polar body during the establishment of SCNT-hESC using MII oocytes obtained from female donors and dermal fibroblasts (DFB) from a 35-year-old adult male. These tetraploid SCNT-hESC lines (CHA-NT1 and CHA-NT3) were identified by the cytogenetic genotyping (91, XXXY,-6, t[2:6] / 92,XXXY,-12,+20) and have shown of indefinite proliferation, but slow speed when compared to euploid SCNT-hESCs. Using the eight Short Tendem Repeat (STR) markers, it was confirmed that both CHA-NT1 and CHA-NT3 lines contain both nuclear and oocyte donor genotypes. These hESCs expressed pluripotency markers and their embryoid bodies (EB) also expressed markers of the three embryonic germ layers and formed teratoma after transplantation into immune deficient mice. This study showed that tetraploidy does not affect the activities of proliferation and differentiation in SCNT-hESC. Therefore, tetraploid hESC lines established after SCNT procedure could be differentiated into various types of cells and could be an useful model for the study of the polyploidy cells in the tissues.

• Nutritional Sciences
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• v.6 no.4
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• pp.216-222
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• 2003

This study investigated the effects of feeding mulberry leaf extracts on lipid composition in rats fed high cholesterol diets. An initial 30-person sensory evaluation of preparations containing various concentrations of mulberry leaf extract showed that a preparation containing 9% mulberry leaf extracts was the most highly preferred. In addition, subsidiary materials of pine needle extracts and mugwort extracts were added to weaken the unpleasant smell of mulberry leaf extract A preparation containing 9% mulberry leaf extract with 3% mugwort extract and 7% pine needle extract was given highest preference scores by the 30-person panel. When comparing the functional ingredients contents of the various preparations of mulberry leaf extracts, such as GABA, DNJ and flavonoids, no significant differences were found as a result of adding subsidiary materials (pine needle and mugwort extracts). Sprague-Dawley male rats weighing l00$\pm$10g were randomly assigned to one normal diet group, and to four high cholesterol diet groups containing 1% cholesterol, to elucidate the functionality of the mulberry leaf extract The four high cholesterol diet groups were classified into: a mulberry leaf extract diet group free of subsidiary materials (EB group); a mulberry extract diet group with pine needle extracts (EP group); a mulberry leaf extract diet group with mugwort extracts (EM group); and a control group (HC group). The mulberry leaf extracts were provided as drinking water; the diet and water were fed ad libitum. Hepatic cholesterol and triglyceride levels were higher, by 279% to 475%, in the high cholesterol groups compared to the normal diet groups, but were significantly lower in the three groups supplied with mulberry leaf extracts, compared with the high cholesterol control. There were no changes in functionality of the mulberry leaf extract preparations due to the addition of subsidiary materials. In conclusion, preparations of mulberry leaf extracts were shown to improve lipid metabolism in rats fed a high cholesterol diet, by reducing hepatic and plasma triglyceride and cholesterol levels. Also human palatability of the mulberry leaf preparation was improved by adding subsidiary materials such as pine needle and mugwort extracts.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.104-104
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• 2003

As an effort to direct differentiation of human embryonic stem (hES, MB03) cells to dopamine-producing neuronal cells, Nurr1 was transfected using conventional transfection protocol into MB03 and examined the expression of tyrosine hydroylase (TH) after differentiation induced by retinoic acid (RA) and ascorbic acid (AA). Experimentally, cells were transfected with linearized Nurr1 cDNA in pcDNA3.1 (+)-hygovernight followed by selection in medium containing hygromycin-B (150 $\mu$/ml). Expression of Nurr1 mRNA was confirmed by RT-PCR and protein by immunocytochemistry in the drug resistant clones. In order to study the effect of Nurr1 protein on the differentiation pattern of ES cells, one of the positive clones (MBNr24) was allowed to form embryoid body (EB) for 2 days and were induced to differentiate for another 4 days using RA (1 $\mu M$) and AA (50 mM) (2-/4+ protocol) followed by selection in N2 medium for 10 or 20 days. After 10 days in N2 medium, cells immunoreactive to anti-GFAP, anti-TH, or anti-NF200 antibodies were 38.8%, 11%, and 20.5%, respectively. After 20 days in N2 medium, cells expressing GFAP, TH, or NF200 were 28%, 15% and 44.8%, respectively but approximately 9% of MB03 expressed TH protein when the cells were induced to differentiate using a similar prorocol, These results suggest that ectopic expression of Nurr1 enhances generation of TH+ cells as well as neuronal cells when hES cells were differentiated by 2-/4+ protocol.