• Title/Summary/Keyword: EAA

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Physico-Chemical Characteristics Evaluation of White Pork Rind and Black Pork Rind (백돈피와 흑돈피의 이화학적 품질특성 평가)

  • Jeon, Ki-Hong;Hwang, Yoon-Seon;Kim, Young-Boong;Choi, Yun-Sang;Kim, Byoung-Mok;Kim, Dong-Wook;Jang, Aera
    • The Korean Journal of Food And Nutrition
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    • v.28 no.4
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    • pp.544-550
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    • 2015
  • In order to determine the material characteristics of pork rinds according to the breeds, the chemical composition, color, pH, collagen contents, shear force, fatty acid and amino acid contents of pork rinds were investigated. White pork rinds (WPR) and black pork rinds (BPR) were evaluated before and after heating to compare the differences between breeds and the effects of heating treatment. In the chemical composition test, the respective moisture contents for WPR and BPR before heating were 49.90% and 53.75% but increased to 60.75% and 61.09% after heating. The test for crude protein and crude fat contents showed lower values after heating but WPR was higher than BPR. In the color test, the L value decreased rapidly with heating, dropping from 68.75 to 45.11 in WPR and from 67.22 to 49.64 in BPR. WPR had a higher L value and a lower a value than BPR before heating but had a higher L value and a lower a value than WPR after heating. pH was significantly higher in WPR than BPR regardless of heating (p<0.05). The collagen content for WPR and BPR was 10.38 g/100 g and 11.54 g/100 g but increased to 12.00 g/100 g in WPR and decreased to 11.40 g/100 g in BPR after heating. The shear force of 26.14 kgf in WPR was significantly higher than 12.89 kgf in BPR before heating (p<0.05), but the values decreased significantly after heating in both WPR and BPR. Linoleic acid in WPR was 17.29%, which was higher than 15.13% in BPR. The USFA for BPR was also higher than WPR. In amino acid composition, the EAA contents in WPR was 7,190 mg%, which was higher than 5,520 mg% in BPR.

Capsaicin Induces Acute Spinal Analgesia and Changes in the Spinal Norepinephrine Level (Capsaicin에 의한 척수 수준에서의 급성 진통효과와 Norepinephrine의 변화)

  • Park, Hyoung-SuP;Park, Kyung-Pyo
    • The Korean Journal of Pharmacology
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    • v.29 no.1
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    • pp.33-41
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    • 1993
  • Central analgesic effect of capsaicin was assessed by the tail flick reflex (TFR) test, using male Sprague-Dawley rats under anesthesia with pentobarbital sodium (induction with 40 mg/kg and maintenance with $4{\sim}8\;mg/kg/hr$). Level of norepinephrine in the spinal cord was also measured. Capsaicin, $35{\sim}150\;{\mu}g$, was injected intrathecally, and the TFR latency was measured before, 10, 30, and 60 minutes after the drug administration. TFR latency was increased 100% or more immediately by intrathecal capsaicin, from 2.9 seconds to the maximum of 7.0 seconds at 10 minute after the drug; P<0.01. The increase in TFR latency was maintained during the course of experiment of 2 hours. Concomitant reduction of NE content in the spinal cord was observed; from 16 ng/mg protein to 7 ng/mg protein. On the other hand, subcutaneous injection of capsaicin of 50 mg/kg did not change the TFR latency although the NE content reduced similarly to the case of intrathecal injection. Pretreatment of the animal with 0.5 mg/kg of MK-801 reversed the increase of TFR latency and NE reduction induced by intrathecal capsaicin. These results suggest that capsaicin causes analgesia at the spinal cord level by activating the excitatory amino acid-NE-dorsal horn interneurons axis of the descending inhibitory pain modulation pathway.

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Isolation and Identification of α-Glucosidase Inhibitory Compounds, Hyperoside, and Isoquercetin from Eleutherococcus senticosus Leaves (가시오갈피(Eleutherococcus senticosus) 잎으로부터 α-Glucosidase의 저해 활성 물질, Hyperoside와 Isoquercetin의 분리 및 구조·동정)

  • Lee, Ki Yeon;Hong, Soo Young;Jeong, Hye Jeong;Lee, Jae Hyoung;Lim, Sang Hyun;Heo, Nam-Kee;Kim, Songmun;Kim, Hee-Yeon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.12
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    • pp.1858-1864
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    • 2014
  • In the present investigation, the anti-diabetic potential of 80% ethanol extract of Eleutherococcus senticosus leaves (EEES) was examined based on ${\alpha}$-glucosidase inhibitory activities. EEES was sequentially fractionated with n-hexane, chloroform, ethyl acetate (EtAOc), n-butanol, and $H_2O$. Of the various fractions, EtAOc fraction effectively inhibited ${\alpha}$-glucosidase activity by 68.05%. Therefore, EtAOc fraction was selected for further isolation and identification studies. EtAOc fraction was separated by medium pressure liquid chromatography with silica and ODS gel to yield eight fractions (EAA~EAH). Based on the results of ${\alpha}$-glucosidase inhibitory activity, EAH fraction was re-chromatographed to yielded four more fractions (EAHA~EAHD). Of these, EAHC fraction showed higher ${\alpha}$-glucosidase inhibitory activity of 93.60%. EAHC fraction was re-chromatographed and yielded EAHCA and EAHCB fractions. Further, identification and chemical structures of these two fractions were analyzed using $^1H$-NMR, $^{13}C$-NMR, and mass spectra data. Based on the results of the spectral data, the isolated compounds were identified as hyperoside and isoquercetin. Results of the present study indicate that the isolated compounds, hyperoside, and isoquercetin from leaves of E. senticosus could be used for the development of new anti-diabetic drugs.

Quality Characteristics of Cultured Olive Flounder Paralichthys olivaceus Fed with Extruded Pellets; I. Comparison of Fatty Acid and Amino Acid Contents (건조 배합사료로 사육한 양식산 넙치의 어체 품질평가; I. 지방산 및 구성아미노산 조성 비교)

  • Jang, Mi-Soon;Kang, Yong-Jin;Kim, Kang-Woong;Kim, Kyoung-Duck;MoonLee, Hae-Young;Heo, Saet-Byeol
    • Korean Journal of Food Science and Technology
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    • v.41 no.1
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    • pp.42-49
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    • 2009
  • This study analyzed the fatty acid and amino acid compositions of dorsal and fin muscles collected from olive flounder cultured using a formula feed(extruded pellets, EP) and compared them to those of fish cultured with a raw fish moist pellet(MP) feed as a control. The olive flounder in this study were cultured for 10 months with either the formulated extruded pellets(FEP), commercial extruded pellets(CEP), or the MP feed, and their average weight was 1.15 kg. The proximate compositions of the dorsal muscle were not different among the groups, whereas in the fin muscles of the fish fed with the MP diet, moisture content was higher and crude lipid content was lower. The major fatty acids in the dorsal and fin muscles of the fish fed with the MP, CEP, and FEP diets were palmitic acid and oleic acid. Finally, no significant differences were observed among the diet groups in the results for amino acid content or the sensory and textural properties of the muscle.

A Comparison on Dry Matter Yield and Feed Value of Winter Forage Crops Cultivated after Rice Harvest in the Central Inland Region (중부내륙지방에서 벼 수확 후 재배한 월동 사료작물들의 생산성 및 사료가치 비교)

  • Lee, Sang Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.1
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    • pp.31-38
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    • 2019
  • This study was carried out to investigate the growth characteristics, yield, and chemical compositions of winter forage crops cultivated after rice harvest in the central inland region. The experimental design was arranged in a randomized block design with three replications. The treatments consisted of 4 species (Rye, Oat, Triticale and Italian ryegrass), and varieties were "Marton", "Donghan", "Shinyoung" and "Kowinnearly", respectively. As a result, dry matter yield and TDN yield were higher in rye than in the other winter forage crops, and lowest in Oat(p<0.05). Crude protein and crude fat content were significantly higher in Italian ryegrass(p<0.05). However, Crude ash and ADF content did not show significant difference among winter forage crops. NDF content was higher in order of Rye > Triticale > Oat > Italian ryegrass(p<0.05). TDN and total amino acid content (EAA+NEAA) were higher in order of winter forage crop with high crude protein content (Italian ryegrass > Oat > Triticale > Rye). Total mineral content was higher in order of Rye > Triticale > Oat > Italian ryegrass(p<0.05), and total free sugar content was higher in order of Oat > Italian Ryegrass > Triticale > Rye(p<0.05). Compared with the results above, Italian ryegrass and Oat are high in crude protein, TDN, amino acid and free sugar content. Rye and Triticale have the merit that feed value is decreased but high yield(dry matter and TDN yield) can be maintained. Therefore, it is advantageous to grow Rye and Triticale as winter forage crops after rice harvest in the central inland region.

Effect of Cryoprotectants on Quality Properties of Chicken Breast Surimi Manufactured by pH Adjustment (냉동변성방지제 조건에 따른 닭가슴살 수리미의 저장특성)

  • Jin, S.K.;Kim, I.S.;Choi, Y.J.;Park, G.B.;Yang, H.S.;Kim, B.G.
    • Journal of Animal Science and Technology
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    • v.50 no.1
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    • pp.99-110
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    • 2008
  • This study was conducted to determine the effect of pH adjustment and the addition of cryoprotectants on the storage properties of chicken breast surimi. Surimi as a control was prepared with Alaska pollack by two times of washing treatment and addition of cryoprotectants(4% sugar, 5% sorbitol and 0.3% polyphosphate). Three types of surimi were manufactured by different cryoprotectant conditions(T1: 5% sorbitol+0.3% polyphosphate, T2: 4% sugar+5% sorbitol+0.3% polyphosphate, T3: 2% salt+4% sugar+5% sorbitol+0.3% polyphosphate) and frozen after adjusting the surimi to pH 11.0. The amino acid and saturated fatty acid composition were significantly higher in the control. EAA(essential amino acid), FAA(amino acid in relation to flavor), SAAA(amino acid in relation to saccarinity) and FRAA (fragrant amino acid) were significantly higher in the control. The TBARS(thiobarbituric acid reactive substances) and VBN(volatile basic nitrogen) were increased with storage times. TBARS and VBN values were significantly higher in the control and T3 than the other treatments. The VBN was significantly higher in control and T3 than other surimi samples. Chicken breast surimi adjusted to pH 11.0 had higher in microorganisms than the control, and T1 sample had the highest total plate count. Sensory evaluations were significantly higher in the control and T3 than the other samples. Especially, the overall acceptability of T3 was similar to the control.

Predicting the Nutritional Value of Seafood Proteins as Measured by Newer In Vitro Model 2. C-PER and DC-PER of Marine Crustacea (수산식품 단백질 품질평가를 위한 새로운 모델 설정 2. 해산 갑각류의 C-PER 및 DC-PER)

  • RYU Hong-Soo;LEE Keun-Woo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.3
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    • pp.219-226
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    • 1986
  • To confirm the application of a newer in vitro assays to determining the nutritional value of marine crustaceans (mainly shrimps and crabs), which have been considered to be highly nutritive depending on their levels of the essential amino acids and digestibility, their C-PERs and DC-PERs were determined and studied the factors influencing their in vitro results. Four species of seawater shrimps and 2 species of seawater crabs were used in this experiment. The in vitro digestibilities showed $83{\sim}86\%$ for raw shrimps and the trypsin indigestibile substrate content (TIS) was ranged from 1.32 to 3.33 mg/g solid expressed quantitatively as mg of purified soybean trypsin inhibitor. The smaller size of shrimps revealed a greater in vitro digestibility and a lower contents of TIS. It was noted that the in vitro digestibility of raw blue crab meat was around $85\%$ while boiled tenner crab meat showed $86\%$ or above, and the leg meat had the greatest in vitro digestibility in the various parts of crab meats. The poor in vitro digestibilities for shrimp's and crab's meat, compared with that of the other seafoods as noted in previous reports, suggest that the drop in pH, due to the change in their freshness during harvesting and frozen storage, resulted in underestimating their digestibilities using four-enzyme digestion technique. The lysine contents in all samples were higher than that of ANRC casein but they contained a slightly lower sulfur-containing amino acids than those in ANRC casein. But the other EAA, such as valine, tyrosine and phenylalanine, were found to be a half as little as that in casein and played a key-factor in calculation of C-PER or DC-PER. It was observed that the value of C-PER and DC-PER for all samples ranged from 2.1 to 2.4, and the predicted digestibilities showed $90\%$ or above in all samples. It was a different results from the fact that the animal proteins bear a higher values and predicted digestibilities than those of C-PER values. The lack of correlation between C-PER and DC-PER values is attributable to the fact that the lower content of valine, tyrosine and phenylalanine, and drop in pH owing to the changes of freshness in marine crustacea proteins. Therefore, if a newer in vitro digestion technique-which are taken into account the pH drop before digestion, TIS content and released free amino acids and/or peptides-developed, C-PER assays can provide more advantages in assessing the protein nutritional value of marine crustacea than any other in vitro assays.

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Effects of Seeding Dates and Growth Periods on the Growth Characteristics, Dry Matter Yield and Feed Value of Corn for Silage in Paddy Field (논토양에서 파종시기와 재배기간이 사일리지용 옥수수의 생육특성, 건물수량 및 사료적 가치에 미치는 영향)

  • Lee, Sang-Moo;Lee, Jae-Hun
    • Journal of Animal Science and Technology
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    • v.52 no.5
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    • pp.441-448
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    • 2010
  • This study was carried out to investigate the effects of the seeding dates and growth periods on the growth characteristics, dry matter yield and feed value of corn for silage in paddy field. The experimental design was arranged in a randomized block design with three replications. Treatments consisted of five seeding dates, 1 May (T1), 8 May (T2), 15 May (T3), 22 May (T4) and 29 May (T5). And harvesting time homologized by August 24. Therefore, growing periods were 115 days (T1), 108 days (T2), 101 days (T3), 94 days (T4) and 87 days (T5), respectively. In maturities at harvest time, T1, T2, T3, T4 and T5 were full dent, early dent, late dough, dough and early dough stage, respectively. Plant height and numbers of root system were highest in T5 (p<0.01), but leaf length was higher in T4 than others (p<0.05). Dead leaf, tip filling degree and ear circle were higher in T1 than others (p<0.05, 0.01). Leaf width, ear height, leaf number, ear length, stem diameter, stem hardness and brix were not significantly different among the treatments. Dry matter yield and TDN yield were higher in the order of T1 > T2 > T3 > T4 > T5 (p<0.01). Crude protein, crude ash, NDF, ADF and crude fiber were significantly higher in T4 (p<0.05, 0.01). But crude fat was the highest in T1, T3 was the lowest as compared to other treatments (p<0.01). Total EAA(essential amino acids) were higher in order of T4 > T3 > T1 > T2 > T5, and total NEAA (nonessential amino acids) were higher in order of T1 > T4 > T2 > T3 > T5, and total amino acids were higher in order of T4 > T1 > T3 > T2 > T5. But no significant differences were found among the treatments. TUFA (total unsaturated fatty acid) and TSFA (total saturated fatty acid) were in order of T1 > T2 > T3 > T5 > T4 (p<0.01). Minerals were the highest in T4 (4,721.25 mg/kg), and T2 (2,970.80 mg/kg) was the lowest as compared to other treatments (p<0.05). Based on the above results, seeding dates could be recommended as early May, and harvest times is yellow ripe stage for qualitative and quantitative production of corn for silage in rice paddy field soil.

Effect of Cryoprotectants on Quality Properties of Chicken Breast Surimi (냉동변성방지제의 종류가 닭가슴살 수리미의 품질 특성에 미치는 영향)

  • Jin, S.K.;Kim, I.S.;Kim, S.J.;Jeong, K.J.;Lee, J.R.;Choi, Y.J.
    • Journal of Animal Science and Technology
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    • v.49 no.6
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    • pp.847-856
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    • 2007
  • This study was conducted to determine the effect of cryoprotectants(sugar, sorbitol, polyphosphate) on quality properties of chicken breast surimi manufactured by pH adjustment(pH 11.0) during frozen storage. Final surimi was divided into experimental units to which the following treatments were randomly assigned: C(Alaska pollack surimi, two times washing, 4% sugar+5% sorbitol+0.3% polyphosphate additive); T1(chicken breast surimi, 0.3% polyphosphate additive); T2(chicken breast surimi, 5% sorbitol +0.3% polyphosphate additive); T3(chicken breast surimi, 4% sugar+5% sorbitol+0.3% polyphosphate additive). All amino acid contents of control were higher than those of all treatments, while T2 was higher in amino acid contents among the treatments. Shear force of all treatments were higher than that of control, but the breaking force, deformation and gel strength were lower. The TBARS(thiobarbituric acid reactive substances) and VBN(volatile basic nitrogen) values of all treatments were lower than those of control, The TBARS values of all treatments were increased with increased storage period. In sensory evaluation, the score of appearance, meat color and overall acceptability of control were higher than those of all treatments, but aroma, juiciness and tenderness were lower than those for all treatments.

Study on the Processing and Compositions of Salted and Dried Mullet Roe (영암산 염건 숭어알의 가공과 조성에 관한 연구)

  • Joe, Sang-June;Rhee, Chong-Ouk;Kim, Dong-Youn
    • Korean Journal of Food Science and Technology
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    • v.21 no.2
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    • pp.242-251
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    • 1989
  • The salted-dried mullet(Mugil japonicus) roe is a kind of traditional food particulary in the area of Young-am gun, Chunnam province. This study was conducted to conform the scientific processing conditions and to evaluate the nutritional quality and changes of major components during storage times. The manufacturing method was that the fresh roe was salted for about 20 hours for the preparation of salted-dried roe, washed by clean waters, drained, shaped a flat piece with 1.2cm thickness by pressing, and spreaded sesame oils on the surface of the salted roe periodically during wind drying for 20 days. The dried roe was blanched in heated water$(80^{\circ}C/3min)$ and packaged the dried product for storages. The fractional compositions of free lipid of wind dried roe were 40% of neutral lipids, 12% of glycolipids and 9% of phospholipids and those of bound lipids were 13% of neutral lipids. 10% of glycolipids and 13% of phospholipids respectively. The major fatty acids of the roe were $C_{16:0}$, $C_{18:0}$, $C_{18:1}$, $C_{18:2}$ and $C_{20:0}$ which was consisted of free and bound lipids in wind drying method during processing and storages. Total amino acids were 99.87g/100g and major amino acids were Glu, Pro, Leu, Lys and CySH and the protein score was average 155% and the chemical score was average 109%. Free amino acids was 1,376mg% that had 50.61% of Pro and the major kinds of those were Tyr and CySH.

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