• 생명과학회지
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• 제21권11호
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• pp.1526-1531
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• 2011

신경세포의 연접후 위치에서 단백질합성은 국소적 연접가소성의 조절에 중요한 역할을 한다. 본 연구에서는 연접후 위치에 eIF들이 존재하는지를 배양한 흰쥐해마신경세포의 면역세포화학적 염색과 immunoblot, 그리고 세제세척실험으로 알아보았다. 단백질해석 시작단계의 초기에 중요한 역할을 하는 eIF4E와 eIF4G, 개시코돈을 찾는 단계에서 중요한 eIF5, 외부자극에 의하여 합성을 시작하게 하는 eIF6, 그리고 불리한 환경에서 해석의 효율을 높여주는 eIF5A 들은 모두 해마신경세포의 연접후에 위치함을 배양한 해마신경세포를 다중초점형광현미경으로 관찰할 수 있었다. 또한 Immunoblot 실험에서도 이들은 연접후치밀질(PSD) 분획에서 검출되었으며, 여러 가지 세제에 의하여 PSD로부터 잘 떨어지지 않는 것으로 보아 PSD와 강하게 결합하고 있음을 알 수 있었다. 본 연구결과는 여러 가지 eIF들이 연접후에 위치하여 다양한 상황에서 단백질합성을 시작하게 할 수 있음을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1100-1105
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• 2005

Previous work has identified a Streptomyces coelicolor gene, rns, encoding a 140 kDa protein (RNase ES) that exhibits the endoribonucleolytic cleavage specificity characteristic of RNase E and confers viability on and allows the propagation of E. coli cells lacking RNase E. Here, we identify a putative S. coelicolor 9S rRNA sequence and sites cleaved by RNase ES. The cleavage of the S. coelicolor 9S rRNA transcript by RNase ES resulted in a 5S rRNA precursor (p5S) that had four and two additional nucleotides at the 5' end and 3' ends of the mature 5S rRNA, respectively. However, despite the similarities between RNase E and RNase ES, these enzymes could accurately process 9S rRNA from just their own bacteria, indicating that these ancient enzymes and the rRNA segments that they attack appear to have co-evolved.

• 한국정보통신학회논문지
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• 제15권10호
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• pp.2209-2216
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• 2011

본 논문에서는 Magnachip $0.18{\mu}m$ 공정을 이용하여 PMIC용 32bit eFuse OTP IP를 설계하였다. eFuse 링크 아래에 N-Well을 두어 프로그램시 eFuse 링크와 p-기판의 VSS가 단락되는 문제점을 해결하였다. 그리고 디코딩된 WERP (WL Enable for Read or Program) 신호가 eFuse OTP 메모리로 바로 입력되는 경우 듀얼 포트 eFuse OTP 메모리 셀의 RWL (Read Word-Line)과 WWL (Write Word-Line)을 선택적으로 활성화해 주는 WL 구동회로를 제안하였다. 또한 BL 프리차징 회로에서 delay chain을 제거하여 제어회로의 레이아웃 면적을 줄였다. 메모리 테스트 장비를 이용하여 제작된 94개의 샘플 die를 측정한 결과 5.5V의 프로그램 전압에서 100%의 수율을 얻었다.

• 농약과학회지
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• 제17권2호
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• pp.140-143
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• 2013

톱다리개미허리노린재의 페로몬 성분인 (E)-2-hexenyl (E)-2-hexenoate (1) 과 (E)-2-hexenyl (Z)-3-hexenoate (2)의 합성에 있어 Steglich 에스테르화 반응의 최적 조건을 검토하였다. (E)-2-헥센산 또는 (Z)-3-헥센산에 대하여 1-1.5 당량의 디사이클로카보다이이미드 (DCC), 1.5-2.0 당량의 (E)-2-헥센놀과 0.1 당량의 4-디메틸아미노피리딘(DMAP)과 반응하였을 때, 각각 76-78% 와 87-91%의 수율로 1과 2를 얻었다.

• Corrosion Science and Technology
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• 제2권5호
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• pp.212-218
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• 2003

Electronic properties of passive films formed on Ti at film formation potentials $(E_f)V_{SCE}$ in pH 8.5 buffer solution and in an artificial seawater were examined through the photocurrent measurement and Mott-Schottky analysis. The passive films formed on Ti in pH 8.5 buffer solution exhibited a n-type semiconductor with a band gap energys $(E_g);E_g^{n=2}=3.4$ eV for nondirect electron transition, and $E_g^{n=0.5}=3.7$ eV for direct electron transition. These band gap values were almost same as those for the passive films formed in artificial seawater, indicating that chloride ion ($Cl^-$ in solution did not affect the electronic structure of the passive film on Ti. $E_g$ for passive films formed on Ti were found to be greater than those ($E_g^{n=0.5}=3.1$ eV, $E_g^{n=2}=3.4$) for a thermal oxide film formed on Ti in air at $400^{\circ}C$. The disorder energy of passive film, determined from the absorption tail of photocurrent spectrum, was much greater than that for the thermal oxide film farmed on Ti in air at $400^{\circ}C$. The greater $E_g$ and the higher disorder energy for the passive film compared with those for the thermal oxide fIlm suggest that the passive film on Ti exhibited more disorded structure than the thermal oxide film. The donor density (about $2.4{\times}10^{20}cm^{-3}$) for the passive film formed in artificial seawater was greater than that (about $20{\times}10^{20}cm^{-3}$) formed in pH 8.5 buffer solution, indicating that $Cl^-$ increased the donor density for the passive film on Ti.

• 대한수의학회지
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• 제36권2호
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• pp.395-403
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• 1996

Monoclonal antibodies(MAbs) against field isolates of the bovine rotavirus A strain(G6), V strain(G10) and reference I-801 strain(G8) were produced and characterized. Six MAbs(4C2, 4D9, 5E1, 5E7, 5D5, 3E4) against A strain had neutralizing activity and reacted only with the G6 bovine rotaviruses determined by fluorescence focus neutralization (FFN) test. Otherwise, five neutralizing MAbs(1G2, 2G6, 5E2, 5E12, 5H7) against I-801 strain neutralized the G6 and G8 bovine rotaviruses. Five non-neutralizing MAbs(5F12, 7F12, 5E11, 2A11, 2B12) were VP6-specific and cross-reacted with all bovine and porcine rotaviruses examined by fluorescence antibody(FA) test. None of the MAbs reacted with bovie viral diarrhea virus(BVDV) and bovine coronavirus(BCV) determined by FA and FFN test.

• 한국물환경학회지
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• 제39권5호
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• pp.396-412
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• 2023

Species from the mayfly genus Ephemera (Order Ephemeroptera) was assessed for their physical microhabitat suitability (namely E. strigata, E. separigata, and E. orientalis-sachalinensis). Probability distribution models (Exponential, Normal, Lognormal, Logistic, Weibull, Gamma, Beta, and Gumbel) based on the data collected from 23,957 sampling units of 6,787 sites in Korea from 2010 to 2021 were used. Mode and standard deviation calculated from the best-fitting models to species distribution along a water depth gradient were 265 cm and 159 cm in E. orientalis-sachalinensis; 10 cm and 83 cm in E. strigata; 20 cm and 15 cm in E. separigata, respectively. The current velocity gradient was 22 cm/s and 40 cm/s in E. orientalis-sachalinensis; 60 cm/s and 53 cm/s in E. strigata; 82 cm/s and 25 cm/s in E. separigata, respectively. The mean diameter (phi scale) of substrate grains were -3.6 and 2.2 in E. orientalis-sachalinensis; -7.4 and 1.5 in E. strigata; -5.8 and 0.9 in E. separigata, respectively. Habitat suitability range of E. orientalis-sachalinensis was estimated to be 161~369 cm (water depth), 5~44 cm/s (current velocity), -5.2~-2.0 (mean diameter); 3~34 cm (water depth), 36~94 cm/s (current velocity), -8.1~-6.3 (mean diameter) for E. strigata; 12~32 cm (water depth), 63~96 cm/s (current velocity), -6.3~-5.2 (mean diameter) for E. separigata. In relative comparison, E. orientalis-sachalinensis was estimated to be rheophobic, eurybathophilic, and eurypsephophilic; E. strigata to be euryrheophilic, bathophobic, and lithophilic; E. separigata to be stenomesorheophilic, stenobathophobic, stenolithophilic.

• 대한수학회보
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• 제47권5호
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• pp.1025-1036
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• 2010

A new class of generalized open sets in a topological space, called e-open sets, is introduced and some properties are obtained by Ekici [6]. This class is contained in the class of $\delta$-semi-preopen (or $\delta-\beta$-open) sets and weaker than both $\delta$-semiopen sets and $\delta$-preopen sets. In order to investigate some different properties we introduce two strong form of e-open sets called e-regular sets and e-$\theta$-open sets. By means of e-$\theta$-open sets we also introduce a new class of functions called strongly $\theta$-e-continuous functions which is a generalization of $\theta$-precontinuous functions. Some characterizations concerning strongly $\theta$-e-continuous functions are obtained.

• Journal of the Korean Wood Science and Technology
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• 제47권5호
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• pp.587-596
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• 2019

This study aimed to determine the antimalarial effect of the Strychnos ligustrina (SLW) wood extracts and to analyze its phytochemicals. The SLW powder samples were macerated with 100% ethanol (E100), 75% ethanol (E75), 50% ethanol (E50), 25% ethanol (E25), and aqueous (A100). The extracts were analyzed by LCMS/MS, and its in-vitro antimalarial activity was tested with Plasmodium falciparum. The results showed that the extract yields of E100, E75, E50, E25, and A100 were 4.3, 5.2, 5.3, 4.7, and 3.6%, respectively. The antimalarial activities of the A100, E25, E50, and E75 extracts were classified as active with $IC_{50}$ values of 38.6, 42.6, 42.9, and $43.7{\mu}g/mL$, respectively. But, the antimalarial activity of the E100 extract was classified as slightly active with $IC_{50}$ values of $87.4{\mu}g/mL$. The dominant compounds contained in the extracts of A100, E25, E50, E75, and E100 was the alkaloid compound, namely brucine with relative concentrations of 24.96, 24.55, 21.33, 11.79, and 11.62%, respectively.

• 공업화학
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• 제24권4호
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• pp.396-401
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• 2013

Benzo[1,2,5]thiadiazole, carbazole 및 phenanthrene을 기본 골격으로 한 교대공중합체인 poly[9-(2-octyl-dodecyl)-9H-carbazole-alt-4,7-di-thiophen-2-yl-benzo[1,2,5]thiadiazole] (PCD20TBT)와 poly[9,10-bis-(2-octyl-dodecyloxy)-phenanthrene-alt-4,7-di-thiophen-2-yl-benzo[1,2,5]thiadiazole] (PN40TBT)을 Suzuki coupling reaction을 이용하여 중합하였다. 합성한 고분자들은 chloroform, chlorobenzene, 1,2-dichlorobenzene, tetrahydrofuran, toluene과 같은 유기용매에 대한 용해도가 우수하였다. PCD20TBT의 최대흡수 파장과 밴드갭은 각각 535 nm와 1.75 eV이고, PN40TBT의 최대흡수 파장과 밴드갭은 각각 560 nm과 1.97 eV이었다. PCD20TBT의 HOMO 및 LUMO 에너지준위는 각각 - 5.11 eV와 - 3.36 eV이고, PN40TBT의 HOMO 및 LUMO 에너지준위는 각각 -5.31 eV와 -3.34 eV이었다. 합성한 고분자와 (6)-1-(3-(methoxycarbonyl)-{5}-1-phenyl[5,6]-fullerene(PCBM)을 1:2의 중량비로 블랜딩하여 제작한 이종접합형태(bulk heterojunction) 태양전지를 제작하였다. PCD20TBT의 광전변환효율은 0.52%, PN40TBT의 광전변환효율은 0.60%이었다. 그리고 소자의 단락 전류밀도, 충진 인자와 개방전압은 PCD20TBT가 각각 $-1.97mA/cm^2$, 38.2%, 0.69 V이며, PN40TBT의 경우 각각 $-1.77mA/cm^2$, 42.9%, 0.79 V이었다.