• Title/Summary/Keyword: E42

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Sea Level Valiability and Eddy in the South Indian Ocean by Multi-satellite Data (Topex/Poseidon, ERS1) (복합위성자료(Topex/Poseidon, ERS1)를 이용한 남인도양의 해수면 변화와 와동류 연구)

  • 윤홍주;서영상
    • Korean Journal of Remote Sensing
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    • v.19 no.4
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    • pp.271-276
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    • 2003
  • Sea level variability and eddy in the Amsterdam-Crozet-Kerguelen region of the South Indian Ocean were studied during 1 year (October 15, 1992~October 15, 1993) using multi-satellite data (Topex/Poseidon, ERS1) produced by signal treatment. We found that sea level variabilities (>10cm) generally exist along the Antarctic Circumpolar Current in the area of 35$^{\circ}$~46$^{\circ}$S and strong sea level variabilities (20~30cm) were especially occurred from the effects of eddy due to bottom topography in two small area: 49$^{\circ}$~57$^{\circ}$E and 38$^{\circ}$~42$^{\circ}$S, and 58$^{\circ}$~64$^{\circ}$E and 42$^{\circ}$~44$^{\circ}$S.

Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells

  • Shen, Yan-Nan;Kim, Yoon-Mo;Yun, Cheol-Heui;Moon, Yang-Soo;Kim, Sang-Hoon
    • BMB Reports
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    • v.42 no.10
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    • pp.691-696
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    • 2009
  • The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.

Evaluation of Usefulness of an m-DIXON Technique during an Abdomen MRI Examination : A Comparison with an e-THRIVE Technique (복부 MRI검사에서 m-DIXON기법의 유용성 평가: e-THRIVE기법과 비교 분석)

  • Lee, Bo-Woo;Park, Myung-Cheol;Lee, Jin-Hoi;Kim, Ki-Jin;Bae, Seok-Hwan
    • Journal of Digital Convergence
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    • v.12 no.10
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    • pp.385-390
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    • 2014
  • This research is conducted to identify whether an m-DIXON technique which is useful for an abdomen MRI examination compared with an existing e-THRIVE technique is a clinically useful or not. There was evaluated quantitative and qualitative to 84 subjects who had abdomen MRI exam due to their liver disease were conducted during a period from September in 2013 to February in 2014. First of all the quantitative evaluation, the m-DIXON technique's SNR was $90.42{\pm}16.90$ and the e-THRIVE technique was $60.42{\pm}11.54$ and the m-DIXON technique's CNR was $52.38{\pm}22.58$ and the e-THRIVE technique was $46.31{\pm}20.25$. Secondly in the qualitative evaluation, the m-DIXON technique's image quality was $4.06{\pm}0.34$, a artifact was $3.64{\pm}0.22$, and fat suppression was $4.16{\pm}0.15$, the e-THRIVE technique's image quality was $3.14{\pm}0.35$, a artifact was $3.06{\pm}0.38$, fat suppression was $3.14{\pm}0.30$. In conclusion, m-DIXON technique for abdomen MRI examination showed superiority over both SNR as a quantitative anaylsis, CNR and a qualitative analysis.

Studies on Esterase of Pieris rapae L. II. Biochemical Properties and Immunological Studies (배추흰나비(Pieris rapae L.)의 esterase에 관한 연구 II. 생화학적 특성 및 면역학적 연구)

  • 박철호;김학열;여성문
    • The Korean Journal of Zoology
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    • v.33 no.3
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    • pp.337-345
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    • 1990
  • The properties of three esterases (E2, E6 and E11) which were previously purified from Pieris rapae L. were determined and physiological role of E6 was inferred using immunological methods. Based on inhibitor study, all of the purified esterases were found to be carboxylesterases (EC 3.1.1.1). The Km values for E2, E6 and E11 were determined to be 6.89 X 10-$^4$M. 3.19 $\times$ l0-$^4$M and 3.69 X 10-$^4$M, respectively. The molecular weights of E2, E6 and E11 were estimated to be 42 KD, 81 KD and 174 KD, respectively. The isoelectric points of E2, E6 and E11 were estimated to be pH 5.54, pH 5.89 and pH 6.50, respectively. The concentration of E6 during development was highest at the late 5th instar larval stage and that according to organs at the same stage was highest in midgut. These results suggest that E6 might be a hydrolase involved in the digestion of dietary lipids.

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Temperature-Dependent Expression of Escherichia coli Thioredoxin Gene

  • Lee, Jin-Joo;Park, Eun-Hee;Ahn, Ki-Sup;Lim, Chang-Jin
    • BMB Reports
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    • v.33 no.2
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    • pp.166-171
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    • 2000
  • Thioredoxin is a multifunctional protein that is ubiquitous in microorganisms, animals and plants. Previously, the expression of the Escherichia coli thioredoxin gene (trxA) was found to be negatively regulated by cAMP. In the present study, the effect of temperature on the expression of the E. coli trxA gene was investigated. In order to examine the temperature effect, the fusion plasmid pCL70 that harbors the E. coli trxA P1P2 promoter was used. The other two fusion plasmids, pJH3 and pMH521 that were constructed in different vectors which harbor the E. coli trxA P2 promoter, were also used. When the E. coli strain MC1061/pCL70 was grown in a rich medium at $25^{\circ}C$, $34^{\circ}C$ and $42^{\circ}C$, the cells grown at $42^{\circ}C$ gave the highest $\beta$-galactosidase activity. The E. coli MC1061/pJH3 and MC1061/pMG521 cells showed increased $\beta$-galactosidase activity after the shift of the culture temperature to $42^{\circ}C$. The wild-type trxA gene of the E. coli MC1061 cells produced much higher thioredoxin activity at the higher temperature. These results support the conclusion that the E. coli trxA gene is regulated in a temperature-dependent manner. Especially the expression from its P2 promoter appeared to be sensitive to temperature.

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Raman Chemical Imaging Technology for Food and Agricultural Applications

  • Qin, Jianwei;Kim, Moon S.;Chao, Kuanglin;Cho, Byoung-Kwan
    • Journal of Biosystems Engineering
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    • v.42 no.3
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    • pp.170-189
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    • 2017
  • Purpose: This paper presents Raman chemical imaging technology for inspecting food and agricultural products. Methods The paper puts emphasis on introducing and demonstrating Raman imaging techniques for practical uses in food analysis. Results & Conclusions: The main topics include Raman scattering principles, Raman spectroscopy measurement techniques (e.g., backscattering Raman spectroscopy, transmission Raman spectroscopy, and spatially offset Raman spectroscopy), Raman image acquisition methods (i.e., point-scan, line-scan, and area-scan methods), Raman imaging instruments (e.g., excitation sources, wavelength separation devices, detectors, imaging systems, and calibration methods), and Raman image processing and analysis techniques (e.g., fluorescence correction, mixture analysis, target identification, spatial mapping, and quantitative analysis). Raman chemical imaging applications for food safety and quality evaluation are also reviewed.