• Title/Summary/Keyword: E4: E6 ratio

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The Effect of Artificial Acid Rain on the Growth and Competition of Echinochloa crus-galli and Digitaria sanguinalis (인공산성비가 돌피와 바랭이의 생장과 경쟁에 미치는 효과)

  • Kim, Jin-Kyung;Do-Soon Cho
    • The Korean Journal of Ecology
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    • v.19 no.1
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    • pp.71-80
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    • 1996
  • The effects of simulated acid rain on the growth and interspecific competition of Echinochloa crus-galli and Digitaria sanguinalis were studied. Changes of growth in height and dry weight were examined and competition between the two species was investigated using the experimental model proposed by de Wit (1960). The experiments were carried out in the greenhouse, and test plants were germinated and grown for 4 weeks and then sprayed with simulated acid rain of pH 5.6, 4.2 and 2.8 for another 8 weeks. In de Wit replacement experiments, the variation of density and the treatment of simulated acid rain showed little effect on the interspecific competition between E. Crus-galli and D.sanguinalis. The ratio diagram, another method to reveal the interrelations between two competing species, always showed the predominance of E. crus-galli in competition, regardless of density and pH. The interspecific competition of the two species, represented by relative yield total (RYT), revealed that intraspecific competition was stronger than interspecific competition in both species.The treatment of simulated acid rain showed little effect on the growth of E. Crus-galli and D. sanguinalis. Height growth of both species did not differ from that of control (pH 5.6), but root/shoot ratio increased with the decrease in pH of simulated acid rain. Growth of tatal dry weight of E. crus-galli and D. sanguinalis was not influenced by the change of pH, except that D.sanguinalis showed a decrease in the total dry weight at pH 2.8 along with a visible sign of white spots on leaves. Physical environmental conditions can exert an effect on the interrelationships between the two species, but this experiment did not show the reversion of competition, and showed that E.crus-gilli was competitively dominant under any environmental conditions given in this study.

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The Effect of Changiga on Immune Cell & Serum OA-specific IgE in BALF in Rat Asthma Model (창이자(蒼耳子)가 제 I 형 알레르기 천식(喘息)모델 흰쥐의 BALF내(內) 면역세포(免疫細胞) 및 혈청(血淸) IgE에 미치는 영향(影響))

  • Lee Byoung-Hee;Lee Sang-Jae;Kim Kwang-Ho
    • Journal of Society of Preventive Korean Medicine
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    • v.5 no.2
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    • pp.93-105
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    • 2001
  • BACKGROUND : Changiga is a hetnal medicine which has been used of the traditional therapeutic agent of asthma. So I examine the effect of Changija on immune Cell&serum OA-specific IgE in BALF in rat asthma model. MATERIAL and METHODS : Rats were sensitized with OA; at day 1 sensitized group and Changiga(CIG) groups were systemically immunized by subcutaneous ingection of 1mg OA and 300mg of Al(OH)3 in a total volume of 2ml. At the same time, 1ml of 0.9% saline containing $6{\times}109$ B. pertussis bacilli was injected by i.p. 14 days, after the systemic immunization, rats received local immunization by inhaling 0.9% saline aerocol containing 2%(wt/vol) OA, A day after local immunization, BAL fluid was collected from the rats. A day after local immunization, rats were orally administered with Changiga extract 14 days, Lymphocyte, CD4+ T-cell CD8+ T-cell counts, CD4+/CD8+ ratio in BALF, change of serum OA-specific IgE level in the peripheral blood were measured and evaluated. RESULT : Changiga showed a suppressive effect on a rat asthme model. Changiga decreased lymphocyte, CD4+ T-cell, CD4+/CD8+ ratio in BALF, serum OA-specific IgE level as compared with the control group, whereas Changiga decreased CD8+ T-cell in BALF with statistical nonsignificance as compared with the control group. CONCLUSION: This study shows that Changiga have a suppressive effect on rat allergic athma model. Changiga would be useful allergic asthma treatment agent.

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A Study on the Effect of Herbal-acupuncture with Mori Ramulus Solution at Joksamni(ST36) on Collagen-induced Arthritis (족삼리(足三里) 상지약침(桑枝藥鍼)이 Collagen-induced arthritis에 미치는 영향)

  • Jeong, Yeong-Don;Yim, Yun-Kyoung;Lee, Hyun
    • Journal of Acupuncture Research
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    • v.23 no.6
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    • pp.29-44
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    • 2006
  • Objectives : The purpose of this study is to observe the effects of Mori Ramulus herbal-acupuncture solution(MR-HAS) on arthritis of mice induced by Collagen II at Joksamni(ST36). Methods : The author performed several experimental items. First, it is the cell survival rate of mice lung fibroblasts. Second, it is the incidence rate of arthritis and arthritis index of CIA. Third, it is the levels of IL-6, $TNF-{\alpha}$, $IFN-{\gamma}$, $IL-1{\beta}$, IgG, IgM and anti-collagen II in serum and the level of $IFN-{\gamma}$, $IFN-{\gamma}/IL-4$ ratio in CIA mouse spleen cell culture. Fourth, it is histological analysis of the mice joint. Fifth, it is expression ratio of CD3e+ to CD19+ cell, CD4+ to CD8+ cell, CD69+/CD3e+ cells, CD11a+/CD19+ cells and CD11b+/Gr-l+ cells and CD4+/CD25+ cells. Results : 1. In the MR-HA, the incidence of arthritis and the arthritis index were significantly decreased. 2. In MR-HA, the levels of IL-6, $IFN-{\gamma}$, $TNF-{\alpha}$, $IL-1{\beta}$, IgG, IgM and anti-collagen II in serum of CIA mice and the level of $IFN-{\gamma}$, IL-4, $IFN-{\gamma}$, IL-4 ratio in CIA mouse spleen cell culture were significantly decreased. 3. In histology, the cartilage destruction and synovial cell proliferation were decreased in the MR-HA, and the collagen fiber expressions in the MR-HA were similar with that of the Normal group. 4. In the MR-HA, the expression ratio of CD3e+ to CD19+ cell and CD4+ to CD8+ cell were similarly maintained as Normal group in lymph nodes, and CD69+/CD3e+ cells and CD11a+/CD19+ cells were decreased in lymph nodes, and CD11b+/Gr-1+ cells and CD4+/CD25+ cells were decreased in synovium. Conclusion : These results suggest that MR-HA at ST36 has an effect to control synovial cell proliferation and cartilage destruction in rheumatoid arthritis, as well as prophylaxis is important to treat rheumatoid arthritis in clinic.

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A Comparative Study on Clinical Effectiveness of Human Chorionic Gonadotropin and Progesterone on Luteal Support in Controlled Ovarian Hyperstimulatian far IVF (체외수정 시술시 과배란 유도에서 Luteal Phase Support에 Human Chorionic Gonadotropin과 Progesterone의 효용성에 관한 비교 연구)

  • Nah, O-Soon;Lee, Sang-Hoon;Bae, Do-Whan
    • Clinical and Experimental Reproductive Medicine
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    • v.21 no.3
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    • pp.233-240
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    • 1994
  • This study was conducted to compare the endocrine milieu, and pregnancy rates in In Vitro Fertilization and Embryo Transfer(IVF-ET) program employing combined with gonadotropin releasing hormone agonist(GnRH-a) and pergonal(LH 75lU+FSH 75lU) when either human chorionic gonadotropin(HCG) or progesterone were used for luteal phase support. A total number of 40 IVF-ET treatment cycles were prospectively studied. Ovarian hyperstimulation method was modified ultrashort protocol using GnRH-a. All patients started Decapeptyl at menstrual cycle day # 2, and HMG was started at # 3 days. When leading follicle was ${\geqq}$18mm or at least two follicles were ${\geqq}$14mm in diameter, HCG 10000lU intramuscularly was injected. After 36 hours HCG administration, oocytes were retrieved as usual guided by transvaginal ultrasound. Embryo were transfered 36-48 hours later. The patient's cycles were prospectively randomized to receive HCG(20cycles) or Progesterone (20cycles) for luteal support. The progesterone group received 25mg 1M starting from the day of ET. The HCG group received 1500IU 1M. on days 0, +2, +5 after ET. Estadiol($E_2$) and Progesterone($P_4$) were measured on the day of oocyte aspiration, ET day, and every 6 days thereafter. Results were follows as; 1. Estradiol, progesterone and LH levels on the day of HCG trigger, retrieved oocytes and number of transfered embryo were not significantly different in both groups. 2. On the day of aspiration and embryo transfered day, $E_2$, $P_4$ level were significantly higher in progesterone group than HCG group(p<0.01). 3. $E_2$, $P_4$ level on 6 days after ET were significantly higher in progesterone group than HCG group(p<0.01). But, $P_4/E_2$ ratio was not different in both groups. 4. $E_2$, $P_4$ level 12 days after ET were decreased abruptly in both groups and higher hormonal level appeared in HCG group(P<0.01). 5. The total pregnancy rate in the HCG group was 40% (8/20) and in the progesterone group 15%(3/20). 6. Comparing the pregnant and nonpregnant cases progesterone group was not different the hormonal status. In HCG group, pregnant cases appeared in higher $P_4$, $P_4/E_2$ ratio than nonpregnanct cases(P<0.01).

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Soil Analysis on Prediction of Consolidation Settlement in Marine Clays (항만점토(港灣粘土)의 압밀심하량(壓密沈下量) 예측(預測)을 위(爲)한 토질분석(土質分析))

  • Kwon, Moo Nam;Son, Kwang Sik;Lee, Sang Ho
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.87-94
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    • 1986
  • This study was performed in order to contemplate their correlations between physical and mechanical properties of the marine clays which were collected from main harbors in Korea. The results obtained are as follows: 1. Most of the soils in experimental districts consist of CH. CL. and ML. and they are considered to be still proceeding. 2. The equations of the relationship between compression index and liquid limit are as, follows: CH : $C_c=0.0137$ (LL-22.60) CL : $C_c=0.0123$ (LL-14.64) 3. The relationship between compression index and initial void ratio appears that the higher the plasticity, the easier the slope of the regression line. The equations are as follows : CH : $C_c=0.431$ ($e_o-0.504$) CH : $C_c=0.471$ ($e_o-0.235$) ML : $C_c=0.641$ ($e_o-0.393$) 4. The equations of the relationship between compression index and natural water content are as follows: CH : $C_c=0.0133$ ($W_n-28.27$) CL : $C_c=0.0225$ ($W_n-23.56$) ML : $C_c=0.0106$ ($W_n-16.42$) 5. The relationship between initial void ratio and natural water content, and compression index is highly positive correlation and the equations are as follows : CH : $C_c=0.301$ ($e_o+0.017W_n-1.05$) CL : $C_c=0.141$ ($e_o+0.0567W_n-1.054$) ML : $C_c=0.421$ ($e_o+0.0214W_n-1.121$) 6. The equations of the relationship between initial void ratio and liquid limit, and compression index are as follows : CH : $C_c=0.36$ ($e_o+0.08LL-0.819$) CL : $C_c=0.269$ ($e_o+0.026LL-0.929$) 7. The cohesion of marine clays is no concerned with the increment of depth. The equations of relationship between cohesion and unconfined compression strength are as follows. CH : qu=1.896C+0.0107 CL : qu=1.849C+0.04.

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Synthesis of cobalt powder with polyol process (폴리올법을 이용한 미립 코발트 분말 합성)

  • Kim, Dong-Jin;Chung, Hun-Saeng;Woo, Sang-Duck;Lee, Jae-Jang
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.11 no.6
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    • pp.290-296
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    • 2001
  • Fin metal particles of uniform shape, narrow size distribution and high purity are increasingly needed for specific uses in high tech industrial applications. Polyol process for the preparation of monodispersed cobalt powders in micron size is described. In this process in inorganic precursor is reduced in liquid polyol under controlled conditions. The reducing agent is the polyol itself and reaction parameters such as the traction temperature, reaction time, addition of protective agent and concentration of the precursor are varied for controling particles size, shape and agglomeration of the metal particles. An optimum synthesis condition was achieved at E.G/DiE/G volume ratio 1:4,Co$(OH)_{2}$polyol molar ratio 0.08~0.32 reaction temperature $210^{\circ}C$, PVP/Co$(OH)_{2}$ molar ratio 0.4.

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Optimal Conditions for Enzymatic Hydrolysate of Snow Crab Chionoecetes japonicus Cooker Effluent Using Response Surface Methodology (RSM을 이용한 홍게(Chionoecetes japonicus) 자숙부산물의 최적 효소가수분해 조건)

  • Baek, Jeong-Hwa;Jeong, Eun-Jeong;Jeon, Seon-Young;Cha, Yong-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.2
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    • pp.99-103
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    • 2011
  • This study was performed to determine the optimal hydrolysis conditions for the production of a flavoring from the precipitation of snow crab cooker effluent (PSCCE) with commercial proteases. Based on cost-per-enzyme activity and sensory evaluations, Flavourzyme$^{(R)}$ 500 MG plus Protamex$^{(R)}$ (1:1 ratio, w/w) were selected as suitable enzymes. Three independent variables consisting of the substrate concentration (S), enzyme-to-substrate ratio (E/S), and hydrolysis time (T) were examined using response surface methodology (RSM). A model equation obtained from RSM was used to predict the degree of hydrolysis (DH) as follows: % DH = 52.285 - 6.371[S] + 5.469[E/S] + 7.599[T] - $5.818[S]^2$ - $5.633[E/S]^2$ - $6.528[T]^2$ - 3.265[E/S][S] - 5.415[T][S] + 4.315[T][E/S]. From the ridge analysis, the conditions favoring the highest degree of hydrolysis were pH 7.45, $55^{\circ}C$, a S of 21.82%, an E/S of 0.50%, and a T of 3.74 h.

Evaluation of the Wear Comfort of Outdoorwear by Skin Wettedness Analyses (Skin Wettedness 분석을 통한 아웃도어웨어의 착용 쾌적성 평가)

  • Jeong, Jeong-Rim;Kim, Hee-Eun
    • Fashion & Textile Research Journal
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    • v.11 no.6
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    • pp.947-952
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    • 2009
  • The purpose of this study is to analyze skin wettedness($w$) used as the rate index of thermal comfort, and to evaluate the wear comfort of outdoorwear. Skin wettedness is widely used to express the degree of thermal comfort. If skin wettedness exceeds a certain threshold, the body feels damp and discomfort. An experiment which consisted of rest(30 min), exercise(30 min) and recovery(20 min) periods was administered in a climate chamber with 10 healthy male participants. Two kinds of outdoorwears made of 100% cotton fabrics (Control) and specially engineered fabrics having feature of quick sweat absorbency and high speed drying fabric (Functional) were evaluated in the experiment. The condition of climate chamber was controlled according to the thermal insulation of 4 kinds of experimental ensembles(E1~E4). Total sweat loss, sweat loss absorbed into clothing and skin temperature were measured. Skin wettedness was calculated from the ratio of evaporative rate to the maximal evaporative capacity. Skin wettedness of 'Functional' was lower than 'Control' in the 3 kinds of ensembles(E1, E2, E4) because the materials of 'Functional' were composed of quick sweat absorbency and high speed drying fabrics, water vapour permeability and waterproof fabrics.

GnRH Agonist Stimulation Test (GAST) for Prediction of Ovarian Response in Controlled Ovarian Stimulation (COH) (난소기능평가를 위한 Gonadotropin Releasing Hormone Agonist Stimulation Test (GAST)의 효용성에 관한 연구)

  • Kim, Mee-Ran;Song, In-Ok;Yeon, Hye-Jeong;Choi, Bum-Chae;Paik, Eun-Chan;Koong, Mi-Kyoung;Song, Il-Pyo;Lee, Jin-Woo;Kang, Inn-Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.26 no.2
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    • pp.163-170
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    • 1999
  • Objectives: The aims of this study are 1) to determine if GAST is a better indicator in predicting ovarian response to COH compared with patient's age or basal FSH level and 2) to evaluate its role in detecting abnormal ovarian response. Design: Prospective study in 118 patients undergoing IVF-ET using GnRH-a short protocol during May-September 1995. Materials and Methods: After blood sampling for basal FSH and estradiol $(E_2)$ on cycle day two, 0.5ml (0.525mg) GnRH agonist ($Suprefact^{(r)}$, Hoechst) was injected subcutaneously. Serum $E_2$ was measured 24 hours later. Initial $E_2$ difference $({\Delta}E_2)$ was defined as the change in $E_2$ on day 3 over the baseline day 2 value. Sixteen patients with ovarian cyst or single ovary or incorrect blood collection time were excluded from the analysis. The patients were divided into three groups by ${\Delta}E_2$; group A (n=30):${\Delta}E_2$<40 pg/ml, group B (n=52): 40 pg/ml${\leq}{\Delta}E_2$<100 pg/ml, group C (n=20): ${\Delta}E_2{\leq}100$ pg/ml. COH was done by GnRH agonist/HMG/hCG and IVF-ET was followed. Ratio of $E_2$ on day of hCG injection over the number of ampules of gonadotropins used ($E_2hCGday$/Amp) was regarded as ovarian responsiveness. Poor ovarian response and overstimulation were defined as $E_2$ hCGday less than 600 pg/ml and greater than 5000 pg/ml, respectively. Results: Mean age $({\pm}SEM)$ in group A, B and C were $33.7{\pm}0.8^*,\;31.5{\pm}0.6\;and\;30.6{\pm}0.5^*$, respectively ($^*$: p<0.05). Mean basal FSH level of group $A(11.1{\pm}1.1mlU/ml)$ was significantly higher than those of $B(7.4{\pm}0.2mIU/ml)$ and C $(6.8{\pm}0.4mIU/ml)$ (p<0.001). Mean $E_2hCGday$ of group A was significantly lower than those of group B or C, i.e., $1402.1{\pm}187.7pg/ml,\;3153.2{\pm}240.0pg/ml,\;4078.8{\pm}306.4pg/ml$ respectively (p<0.0001). The number of ampules of gonadotropins used in group A was significantly greater than those in group B or C: $38.6{\pm}2.3,\;24.2{\pm}1.1\;and\;18.5{\pm}1.0$ (p<0.0001). The number of oocytes retrieved in group A was significantly smaller than those in group B or C: $6.4{\pm}1.1,\;15.5{\pm}1.1\;and\;18.6{\pm}1.6$, respectively (p<0.0001). By stepwise multiple regression, only ${\Delta}E_2$ showed a significant correlation (r=0.68, p<0.0001) with $E_2HCGday$/Amp, while age or basal FSH level were not significant. Likewise, only ${\Delta}E_2$ correlated significantly with the number of oocytes retrieved (r=0.57, p<0.001). All four patients whose COH was canceled due to poor ovarian response belonged to group A only (Fisher's exact test, p<0.01). Whereas none of 30 patients in group A (0%) had overstimulation, 14 patients among 72 patients (19.4%) in group B and C had overstimulation (Fisher's exact test, p<0.01). Conclusions: These data suggest that initial $E_2$ difference after GAST may be a better prognostic indicator of ovarian response to COH than age or basal FSH level. Since initial $E_2$ difference demonstrates significant association with abnormal ovarian response such as poor ovarian response necessitating cycle cancellation or overstimulation, GAST may be helpful in monitoring and consultation of patients during COH in IVF-ET cycle.

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A new species of the genus Eurycletodes Sars G.O., 1909 (Copepoda: Harpacticoida: Argestidae) from South Sea of Korea

  • Bang, Hyun Woo
    • Journal of Species Research
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    • v.9 no.4
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    • pp.480-488
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    • 2020
  • The genus Eurycletodes Sars, 1909 is reported for the first time in Korea. A new species of the genus Eurycletodes was collected from Hansando Island, South Sea of Korea. So far, Eurycletodes comprises 27 species, with no previously recorded species reported in East Asia, including Korea. Eurycletodes (Oligocletodes) vadumus sp. nov. is morphologically most closely related to E. (O.) denticulatus Por, 1967, E. (O.) aculeatus Sars, 1920, and E. (O.) diva Menzel, 2011, but clearly distinguishable from the others based on the following morphological characteristics: absence of A2 exopodal seta, A1 last segment with two inner setae, P4 exp-3 proximal inner seta modified, the ratio of length and of caudal rami (1.6 times), and mandibular palp with three setae. Together with the new species, the number of valid species in this genus has risen to 28. A key to species of the genus Eurycletodes is provided.