• Journal of Korean Biological Nursing Science
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• v.13 no.1
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• pp.1-7
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• 2011

Purpose: The purpose of this study was to compare the hypertrophic effects of low-intensity exercise on weight, myofibrillar protein content and Type I, II fiber cross-sectional area of hindlimb muscles of rats between every other day exercise and every day exercise. Methods: Adult male Sprague-Dawley rats were assigned to 1 of 3 groups: control group (C, n=6), experimental group 1 (E1, n=7) and experimental group 2 (E2, n=7). Rats in E1 group had 7 sessions (every other day) and those in E2 group had 14 sessions (every day) of exercise in which they ran on a treadmill for 30 min/day at 10 m/min. Results: Muscle weight, cross-sectional area of type I fiber and myofibrillar protein content of soleus and myofibrillar protein content of plantaris in E1 group, and myofibrillar protein content of soleus and cross-sectional area of type I fiber of plantaris in E2 group were greater than those in C group. Cross-sectional area of type I fiber of soleus of E1 group was higher than E2 group while cross-sectional area of type I fiber of plantaris of E2 group was higher than E1 group. Conclusion: Hypertrophy of hindlimb muscles occurs from every other day exercise similar to every day exercise.

• Korean Journal of Animal Reproduction
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• v.27 no.2
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• pp.143-152
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• 2003

The objective of this study were to investigate the effects of Se and Vit. E administration on blood chemical values and Vit. E concentrations of serum in calves born from Hanwoo dams injected Se and Vit. E at late pregnancying periods. The effects of Se and Vit. E administration of blood chemical values in calves born from Hanwoo dams injected Se and Vit. E at the last month of pregnancying periods were examined. In albumin concentration of blood, the Vit. E 2000IU group were slightly higher than other groups and the cholesterol concentrations were also higher in Vit. E 15001u group than other groups. But, another components examined of blood chemical values were not significantly different in all experimental groups. The blood chemical values in calves born from Hanwoo dams administrated Se and Vit. E at two months before parturition were examined. However, there were not significantly different in all experimental groups in concentrations of albumin, cholesterol, BUN, creatinine, glucose, inorganic phosphorous, calcium, total protein and triglycerides. The concentrations of serum Vit. E in calves born from Hanwoo dams administrated Se and Vit. E at 30 and 15 days before parturition were examined. The concentrations of Vit. E in serum were higher in treatment groups than in control group(P>0.05). The concentrations of serum Vit. E in calves born from Hanwoo dams administrated Se and Vit. E at 60 days before parturition were examined, the concentrations of Vit. E in serum were slightly higher in groups administrated than in control group(P>0.05), but there were not significantly difference in all experimental groups.

• Korean Journal of Microbiology
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• v.22 no.3
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• pp.183-189
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• 1984

In order to find specific acting site of Rec A protein in plasmic polymerization in E. coli, we randomly deleted various part of pEC-3 (a derivative of pBR322) with SI nuclease treatment. Self-ligated plasmids were introduced into E. coli WA802(Rec $A^+$). A number of colonies were analyzed if they contained monomeric or polymeric plasmids by gel electrophoresis. The plasmid (pEC-43), which was deleted the region of tetracycline gene, revealed only monomeric form in Rec $A^+$ E. coli. When two plasmids, pEC-3 and pEC-43, were co-transformed in the same E. coli, the original pEC-3 showed polymerization but pEC-43 revealed monomeric form only. These results suggest that Rec A protein requires the specific site for polymerization.

• Journal of Nutrition and Health
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• v.53 no.4
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• pp.347-355
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• 2020

Purpose: Zinc is known to be associated with osteoblast proliferation and differentiation. Osterix as zinc-finger transcription factor is also related to osteoblast differentiation and bone formation. In the present study, we aimed to investigate whether zinc modulates osterix gene and protein expression in osteoblastic MC3T3-E1 cells. Methods: MC3T3-E1 cells were cultured in zinc-dependent concentrations (0, 0.5, 1, 5, or 15 µM Zn), along with osteogenic control (normal osteogenic medium) for 1 and 3 days. The gene and protein expression levels of osterix were analyzed by real-time reverse transcription polymerase chain reaction and Western blotting, respectively. Results: Zinc increased osteoblast proliferation in a concentration-dependent manner at day 1 and 3. Similarly, zinc increased the activity of osteoblast marker enzyme alkaline phosphatase in cells and media in a zinc concentration-dependent manner. Moreover, our results showed that the pattern of osterix gene expression by zinc was down-regulated within the low levels of zinc treatments (0.5-1 µM) at day 1, but it was up-regulated after extended culture period at day 3. Osterix protein expression by zinc showed the similar pattern of gene expression, which down-regulated by low zinc levels at day 1 and up-regulated back at day 3 as the early stage of osteoblast differentiation. Conclusion: Our results suggest that zinc modulates osterix gene and protein expression in osteoblasts, particularly in low level of zinc at early stage of osteoblast differentiation period.

• Journal of Life Science
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• v.14 no.2
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• pp.325-330
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• 2004

The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. To examine the extent of conservation of structure and function of RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (Homologue of RAD3 gene). The over-expressed HRD3 protein was estimated to be a 75 kDa in size which is in good agreement with the estimated by the nucleotide sequence of the cloned gene. Two-dimensional gel electrophoresis showed that a number of other protein spots dramatically disappeared when the HRD3 protein was overexpressed. The overexpressed RAD3 protein showed a toxicity in E. coli host, suggesting that this protein may be involved in the inhibition of protein synthesis and/or degradation of host protein. To determine which part of HRD3 gene contributes to the toxicity in E. coli, various fusion plasmids containing a partial sequence of HRD3 and lac'Z gene were constructed. These results suggest that the C-terminal domain of HRD3 protein may be important for both toxic effect in E. coli and for its role in DNA repair in S. pombe.

• Korean Journal of Materials Research
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• v.28 no.3
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• pp.182-188
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• 2018

Nitrogen (N)-doped protein-based carbon as platinum (Pt) catalyst supports from tofu for oxygen reduction reactions are synthesized using a carbonization and reduction method. We successfully prepare 5 wt% Pt@N-doped protein-based carbon, 10 wt% Pt@N-doped protein-based carbon, and 20 wt% Pt@N-doped protein-based carbon. The morphology and structure of the samples are characterized by field emission scanning electron microscopy and transmission electron micro scopy, and crystllinities and chemical bonding are identified using X-ray diffraction and X-ray photoelectron spectroscopy. The oxygen reduction reaction are measured using a linear sweep voltammogram and cyclic voltammetry. Among the samples, 10 wt% Pt@N-doped protein-based carbon exhibits exellent electrochemical performance with a high onset potential of 0.62 V, a high $E_{1/2}$ of 0.55 V, and a low ${\Delta}E_{1/2}=0.32mV$. Specifically, as compared to the commercial Pt/C, the 10 wt% Pt@N-doped protein-based carbon had a similar oxygen reduction reaction perfomance and improved electrochemical stability.

• BMB Reports
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• v.42 no.10
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• pp.691-696
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• 2009

The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.2
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• pp.192-196
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• 2005

A study was conducted to monitor the changes in serum protein profile, cholesterol and blood glucose during endotoxic shock in buffalo calves and also to assess the role of prophylactic supplementation of vitamin E and selenium in alleviating the endotoxic effects. Fifteen male buffalo calves (6-8 months of age) were divided into three groups: Group I (control)-infused with 0.9% saline solution; Group II-infused with E. coli endotoxin at 5${\mu}g/kg$ body weight in normal saline solution; Group III- supplemented prophylactically with 250 mg vitamin E and 7.5 mg selenium by i/m injections at weekly intervals for one month prior to the induction of endotoxic shock. The blood samples were collected at 0, 1, 3, 6, 9, 12, 24, 48 and 72 h after the induction of shock. Endotoxin caused a significant (p<0.05) hypoproteinemia from 3-12 h post infusion in group II but this hypoproteinemia was less pronounced and only from 3-9 h post infusion in vitamin E and selenium supplemented calves. Hypoglycemia was observed in group II from 3-24 h and blood glucose level returned to normal at 72 h. However hypoglycemia was mild in group III and blood glucose returned to normal at 48h. Hypocholesterolaemia and hypoalbuminemia were found in both groups II and III but these changes were less pronounced in group III i.e. vitamin E and Se supplemented calves. Serum electrophoretic protein patterns of group III were quite similar to those of control group but animals of group II had different electrophoretic pattern. It was concluded that the antioxidant effects of vitamin E and Se prevent the liver against oxidative stress during endotoxic shock.

• Fisheries and Aquatic Sciences
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• v.13 no.2
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• pp.118-126
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• 2010

A feeding trial was conducted to investigate the effects of different levels of dietary n-3 highly unsaturated fatty acids (HUFA) (1.1-5.6%) and vitamin E (70 and 400 mg/kg) on the growth and body composition of juvenile rockfish. Six isonitrogenous (45% crude protein) and isolipidic (17% crude lipid) diets were formulated to contain graded levels of n-3 HUFA and vitamin E. Diets 1, 2 and 3 consist of 400 mg vitamin E/kg diet with graded levels of 1.1, 3.0, and 5.6% n-3 HUFA, respectively. Graded levels of n-3 HUFA (1.1, 3.0, and 4.0%) were added in diets 4, 5 and 6, respectively, containing 70 mg vitamin E/kg diet each. At the end of feeding trial, growth performance of rockfish was affected by neither dietary n-3 HUFA nor vitamin E levels. Feed efficiency and hepatosomatic index were slightly decreased (P<0.05) with increment of dietary n-3 HUFA at each dietary vitamin E level. Dietary vitamin E and n-3 HUFA levels did not affect proximate composition and vitamin E concentration in the dorsal muscle of rockfish. Liver moisture and crude protein contents positively related to dietary n-3 HUFA levels. Liver lipid content and hematocrit value were significantly decreased (P<0.05) by increasing dietary n-3 HUFA levels. Eicosapentaenoic acid (20:5n-3; EPA) and docosahexaenoic acid (22:6n-3; DHA) concentrations in the dorsal muscle significantly correlated to dietary n-3 HUFA levels, except for fish fed the diet 6 containing 4% n-3 HUFA and 70 mg vitamin E/kg diet. EPA concentration in the dorsal muscle of fish fed the diet 6 was significantly lower than that of fish fed the diets 2, 3 and 5. The present findings suggest that feeding of diets containing excessive n-3 HUFA level with varying addition of vitamin E may alter fatty acid composition in the dorsal muscle, but do not affect growth of juvenile rockfish.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.466-469
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• 2004

The recombinant bioluminescent Escherichia coli strains, DPD2511 and TV 1061 containing the katG and grpE promoters, respectively, from Vibrio fischeri fused to luxCDABE, were used to detect the adaptive and repair responses to oxidative damage caused by hydrogen peroxide $(H_2O_2)$, and protein damage due to phenol. The response ratio, represented as the bioluminescence induced in subsequent inductions of DPD2511 and TV1061 with the mother cells previously induced by each chemical, i.e., $H_2O_2$ and phenol during the previous induction stage, decreased suddenly compared with the ratio of the control culture of each strain, meaning there is a possible adaptive response to stress caused by chemicals. Protein damage due to phenol was completely repaired by the second culturing after the initial induction, as was oxidative damage caused by $H_2O_2$ which was also rapidly repaired, as detected by the recovery of bioluminescence level. This result suggests that E. coli promptly adapt and repair oxidative and protein damage by $H_2O_2$ and phenol completely.