• Speech Sciences
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• v.1
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• pp.201-211
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• 1997

Acoustically a naturally-spoken vowel is composed of five formants. However, the acoustic quality of a vowel is known to be mostly determined by $F_1\;and\;F_2$. The main purpose of this study was to examine how synthesized vowels with $F_1\;and\;F_2$ are perceived by Korean native speakers. In addion, we are interested in finding whether the synthesized vowels are perceived differently by standard Korean speakers and Kyungnam regional dialect speakers. In the experiment 9 Seoul standard Korean speakers and 9 Kyungnam dialect speakers heard 536 vowels synthesized in vowel space with $F_1\;by\;F_2$ and categorized them into one of 10 Korean vowels. The resultant vowel map showed that each Korean vowel occupies an unique area in the two-dimensional vowel space of $F_1\;by\;F_2$, and confirmed that $F_1\;and\;F_2$ play important roles in the perception of vowels. The results also showed that the Seoul speakers and the Kyungnam speakers perceive the synthesized vowels differently. For example, /e/ versus /$\varepsilon$/ contrast, /y/, and /$\phi$/ are perceived differently by the Seoul speakers, whereas they were perceptually confused by the Kyungnam speakers. These results might be due to the different vowel systems of the standard Korean and the Kyungnam regional dialect. While the latter uses a six-vowel system which has no /e/ vs /$/ contrast, /v/ vs /i/ contrast, /y/, and /$\phi$/, the former recognizes these as different vowels. This result suggests that the vowel system of differing dialect restricts the perception of the Korean vowels. Unexpectedly /i/ does not occupy any area in the vowel apace. This result suggests that /i/ cannot be synthesized without $F_3$.

• Radiation Oncology Journal
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• v.16 no.4
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• pp.377-388
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• 1998

Purpose : To evaluate changes in expression of cell cycle related genes during apoptosis induced in HL60 cells by X-irradiation to understand molecular biologic aspects in mechanism of radiation therapy. Material and Methods : HL-60 cell line (promyelocytic leukemia cell line) was grown in culture media and irradiated with 8 Gr by linear accelerator (6 MV X-ray). At various times after irradiation, ranging from 3 to 48 hours were analyzed apoptotic DNA fragmentation assay for apoptosis and by western blot analysis and semi-quantitative RT-PCR for expression of cell cycle related genes (cyclin A, cyclin B, cyclin C, cyclin Dl, cyclin E, cdc2, CDK2, CDK4, $p16^{INK4a}$, $p21^{WAF1}$, $p27^{KIP1}$, E2F, PCNA and Rb). Results : X-irradiation (8 Gy) induced apoptosis in HL-60 cell line. Cycline A protein increased after reaching its peak 48 h after radiation delivery and cyclin E, E2F, CDK2 and RB protein increased then decreased after radiation. Radiation induced up-regulation of the expression of E2F is due to mostly increase of Phosphorylated retinoblastoma proteins (ppRb). Cyclin Dl, PCNA, CDC2, CDK4 and $p16^{INK4a}$ protein underwent no significant change at any times after irradiation. There was not detected $p21^{WAF1}$ and $p27^{KIP1}$ protein. Cyclin A, B, C mRNA decreased immediately after radiation and then increased at 12 h after radiation. Cyclin Dl mRNA increased immediately and then decreased at 48 h after radiation. After radiation, cyclin E mRNA decreased with the lapse of time. CDK2 mRNA decreased at 3h and increased at eh after radiation. CDK4 mRNA rapidly increased at 6 to 12 h after radiation. There was no change of expression of $p16^{INK4a}$ and not detected in expressin of $p21^{WAF1}$ and $p27^{KIP1}$ mRNA. Conclusion : We suggest that entry into S phase may contribute to apoptosis of HL60 cells induced by irradiation. Increase of ppRb and decrease of pRb protein are related with radiation induced auoptosis of HL60 cells and tosis of HL60 cells induced by irradiation. Increase of ppRb and decrease of PRb protein are related with radiation induced apoptosis of HL60 cells and this may be associated with induction of E2F and cyclinE/CDK2. These results support that $p21^{WAF1}$ and $p27^{KIP1}$ are not related with radiation induced-apoptosis.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.2
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• pp.176-182
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• 2019

Objective: This study was conducted to investigate basic information on genetic structure and characteristics of Limousin population in Hungary. Obtained results will be taken into consideration when adopting the new breeding strategy by the Association of Hungarian Limousin and Blonde d'Aquitaine Breeders (AHLBB). Methods: Genetic diversity and phylogenetic relationship of 3,443 Limousin cattle from 16 different herds were investigated by performing genotyping using 18 microsatellite markers. Amplified DNA was genotyped using an automated genetic analyzer. Results: Mean of effective alleles ($n_e$) of the populations was 3.77. Population C had the lowest number of effective alleles (3.01) and the lowest inbreeding coefficient ($F_{IS}$) value (-0.15). Principal component analysis of estimated genetic distance ($F_{ST}$) values (p<0.000) revealed two herds (C and E) distinct from the majority of other Limousin herds. The pairwise $F_{ST}$ values of population C compared to the others (0.066 to 0.120) fell into the range of moderate genetic distance: 0.050 to 0.150, while population E displayed also moderate genetic distance ($F_{ST}$ values in range 0.052 to 0.064) but only to six populations (G, H, J, L, N, and P). $F_{ST(C-E)}$ was 0.148, all other pairs -excluding C and E herds- displayed low genetic distance ($F_{ST}$<0.049). Population D, F, I, J, K, L, N, O, and P carried private alleles, which alleles belonged to 1.1% of the individuals. Most probable number of clusters (K) were 2 and 7 determined by Structure and BAPS software. Conclusion: This study showed useful genetic diversity and phylogenetic relationship data that can be utilized for the development of a new breeding strategy by AHLBB. The results presented could also contribute to the proper selection of animals for further whole genome scan studies of Limousins.

• Journal of the Korea Furniture Society
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• v.11 no.2
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• pp.73-78
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• 2000

This study was carried out to measure formaldehyde emission with the passing of two years from plywood, sliver-board and strand-board bonded with urea resins which were made of 6 f/U molar ratios. The urea resins were manufactured by six kinds of formaldehyde/urea molar ratio of 1.0, 1.2, 1.4, 1.6, 1.8 and 2.0. 1. The plywood with molar ratio of 1.0 satisfied the KS F3101 $F_2$ directly after manufacture. The plywood with molar ratio of 1.2 satisfied m 3 days. The plywood with molar ratio of 1.4 satisfied the $F_3$ in 3 days and the $F_2$ in 600 days. And the plywood with molar ratio of 1.8 and 2.0 satisfied the $F_3$ in 365 days, but didn't satisfy the $F_2$ in 730 days. 2. Sliver-board with molar ratio of 1.0 and 1.2 satisfied the KS F3104 $E_2$ right after manufacture. Sliver-board with molar ratio of 1.4 and 1.6 satisfied in 150 and 360 days, respectively. Sliver-board with molar ratio of 1.8 and 2.0 satisfied in 730 days. 3. Strand-board with molar ratio of 1.0 and 1.2 satisfied the KS F3104$ E_2$ directly after manufacture. Strand-board with molar ratio of 1.4 and 1.6 satisfied in 150 days. But Strand-board with molar ratio of 1.8 and 2.0 didn't satisfied in 730 days.

• Bulletin of the Society of Naval Architects of Korea
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• v.6 no.1
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• pp.35-42
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• 1969

Penetrations of arc welding electrades are different by changing welding conditions, welding speed of travel and current, even though quite same ones. Changing status of penetration is studied by changing welding speed of travel keeping welding current constant, at first, and by changing welding current keeping welding speed of travel which was obtained prior experiment constant, afterward. The F-100 4mm $\phi$ rods, E4301 class, which covering substance is mainly ilmenite and a domestic representative product of this class electrode, are used for this experiment. The Marquette's No.130 5/32"(4mm)$\phi$ rods, E6011 class, and No. 140 5/32"(4mm)$\phi$, E6013 class, which were produced in the United Stated, are used to compare penetration with F-100 at same welding conditions. The covering substance of Marquette's No. 130 rods is mainly cellulose, and belong to deep penetration arc welding electrodes, the covering material of another rods is titania, and a kind f shallow penetration rods. The result of this study is as follow : penetration of F-100, domestic ilmentite covering electrade, E4301 class, is between E6011 and E6013 of the United States products. At the lower range of welding current, penetration is closer to E6013 than E6011, at the higher range, closer to E6011 than another; and the value of penetration ranges $1.65{\sim}2.70mm$ for 4mm$\phi$ rods in the adequate current range. The changing status of penetration is showed by following experimental formula. $P=KE_v^{-\frac{2}{5}}1^{\frac{3}{2}}$

• MALSORI
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• no.58
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• pp.19-34
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• 2006

The present study investigates the vowels of Bahasa Malaysia and Bahasa Indonesia in terms of the first two formant frequencies and provides a three-dimensional formant chart of vowels by plotting F1, F2, and the frequency of datapoints on 4 different scales: Hz, mel, bark, and the number of ERB. For this study, we recruited 30 male native speakers of Bahasa Malaysia and Bahasa Indonesia (15 each) which include 6 vowels (i, e, a, o, u, e) in various contexts. The three-dimensional formant chart showed geophysics of vowel space, such that mountain peaks stand in particular locations with a higher frequency of occurrence of datapoints. The geophysics of vowel space may shed lights on the perceptual structure of vowel space. The results also showed that vowels in utterance-final positions have a significantly higher F1 and a significantly lower F2 than those in utterance-medial or utterance-initial positions, which means that vowels in utterance-final positions are more back and lower in vowel space than those in utterance-medial or utterance-initial positions.

• Bulletin of the Korean Chemical Society
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• v.21 no.8
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• pp.823-827
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• 2000

Gas-Phase reactions of methyl and ethyl nitrites with anionic nucleophiles of SH-, F- and OH- are investigated theoretically at the MP2/6-311+G* level. The SN2 processes are all highly exothermic and proceed with a typ-icaI double-weIl reaction coordinate profile. The elimination reactions of methyl nitrite with SH- and F- are double-well energy surface processes,with stabilizedproduct complexes of NO-...H2S and NO-...HF, pro-ceeding by an E1 cb-like E2 mechanism. The $\beta-elimination$ of ethyl nitrite is an E2 type process. The $\alpha-elimi-nation$ reactions of methyl and ethyl nitrites with OH- have triple-well energy profiles of Elcb pathway with an $\alpha-carbanion$ intermediate which is stabilized bythe vicinal $nc\alpha-{\sigma}*o-N$ charge transfer interactions. CompIex-ation ofmethyl carbanion with HF seems to provide a stable intermediate within a triple-well energy profile of El cb channel in the reaction of F- with methyl nitrite.

• BMB Reports
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• v.32 no.2
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• pp.189-195
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• 1999

From the Panax ginseng chloroplast, the psbE and psbF genes, encoding the $\alpha$- and $\beta$-subunits of cytochrome b-559 of the photosystem II reaction center, respectively, were cloned and characterized. The psbE and psbF genes were composed of 252 and 117 nucleotides, respectively. The deduced amino acid sequence of the $\alpha$-subunits showed 95%, 93%, and 91% homology to monocots, dicots, and liverwort, respectively, whereas the $\beta$-subunits showed approximately 98% to 95% homology to the same species. Southern blot analysis revealed that a single copy of the psbEF gene exists in the chloroplast plastid. Northern blot analysis indicated that the psbE and psbF genes are cotranscribed as a polycistron.

• Journal of the Chungcheong Mathematical Society
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• v.13 no.2
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• pp.1-7
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• 2001

In this paper, we show that for a homotopically periodic (i.e., $fk{\simeq}id$, for some $k{\geq}1$) self map f of a Seifert 3-manifold with $P{\widetilde{SL(2,}}\mathbb{R})$-geometry, N(f) = L(f) = 0.

• Journal of the Korean Magnetics Society
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• v.13 no.1
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• pp.36-40
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• 2003

We have grown D $y_{x}$B $i_{3-x}$F $e_{5}$ $O_{12}$ (x = 0.5,1.0, 1.5,2.0) magnetic garnet thin films upon $Al_2$O3i and GGG substrate using Pechini process. The annealing temperature to get single phase D $y_{x}$B $i_{3-x}$F $e_{5}$ $O_{12}$ garnet is dependent on substrate, i.e. the annealing temperature for GGG substrate il 5$0^{\circ}C$ lower than that for $Al_2$ $O_3$ substrate. The grains of garnet thin film grown on GGG (111) plane align along [111] direction, and in this case the hysteresis curve does not saturate up to H : 5000 Oe. We attribute this phenomenon to rotation magnetization process. The maximum amount of Bi substitution in polycrystalline D $y_{x}$B $i_{3-x}$F $e_{5}$ $O_{12}$ thin film prepared by Pechini process is restricted to 2.0 Bi atom/unit cell, and this value is less than that in single garnet crystall grown by LPE method.own by LPE method.ethod.