• Ocean and Polar Research
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• v.24 no.3
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• pp.267-278
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• 2002

This study investigated the spatial characteristics of warming trends and the dipole-like pattern of temperature field in the Antarctic Peninsula using surface air temperature (SAT) of 10 stations in the vicinity of the Antarctic Peninsula. SAT data for the 1962-2001 period at 6 stations (Rothera, Faraday/Vernadsky, Bellingshausen, Orcadas, Esperanza, Halley) revealed in general the larger warming trends in autumn and winter except for Halley. The largest warming was shown for August in the west side of the Peninsula (more than $0.9^{\circ}C/decade$). On the other hand, the recent 14-year SAT data showed the strong warming trends at 9 stations except for Halley in the earlier period (April-June) than August for the 1962-2001 period. The largest warming appeared in May at Esperanza and Butler Island. SAT of the two sides showed significant positive correlations over most of the period except for the mid- and the late 1970s, in which significant negative correlations were found. In the correlation analysis between SAT and sea surface temperature (SST) anomalies in the NINO 3.4 region, strong negative correlation was found in the west side of the Peninsula. Details of the correlation analysis exhibited that the negative correlation was significantly strong from the early 1980s to the mid-1990s. However, it was difficult to find significant correlations of ENSO with SAT in the east side of the Peninsula. So, in this study it failed to find out clearly the out-of-phase relationship of SAT across the Antarctic Peninsula.

• Journal of Microbiology and Biotechnology
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• v.28 no.10
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• pp.1581-1588
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• 2018

The growth of lactic acid bacteria (LAB) generates a high number of metabolites related to aromas and flavors in fermented dairy foods. These microbial proteases are involved in protein hydrolysis that produces necessary peptides for their growth and releases different molecules of interest, like bioactive peptides, during their activity. Each genus in particular has its own proteolytic system to hydrolyze the necessary proteins to meet its requirements. This review aims to highlight the differences between the proteolytic systems of Streptococcus thermophilus and other lactic acid bacteria (Lactococcus and Lactobacillus) since they are microorganisms that are frequently used in combination with other LAB in the elaboration of fermented dairy products. Based on genetic studies and in vitro and in vivo tests, the proteolytic system of Streptococcus thermophilus has been divided into three parts: 1) a serine proteinase linked to the cellular wall that is activated in the absence of glutamine and methionine; 2) the transport of peptides and oligopeptides, which are integrated in both the Dpp system and the Ami system, respectively; according to this, it is worth mentioning that the Ami system is able to transport peptides with up to 23 amino acids while the Opp system of Lactococcus or Lactobacillus transports chains with less than 13 amino acids; and finally, 3) peptide hydrolysis by intracellular peptidases, including a group of three exclusive of S. thermophilus capable of releasing either aromatic amino acids or peptides with aromatic amino acids.

• Animal Bioscience
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• v.37 no.3
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• pp.522-535
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• 2024

Objective: Transition period is considered from 3 weeks prepartum to 3 weeks postpartum, characterized with dramatic events (endocrine, metabolic, and physiological) leading to occurrence of production diseases (negative energy balance/ketosis, milk fever etc). The objectives of our study were to analyze the periodic concentration of serum beta-hydroxy butyric acid (BHBA), glucose and oxidative markers along with identification, and validation of the putative markers of negative energy balance in buffaloes using in-silico and quantitative real time-polymerase chain reaction (qRT-PCR) assay. Methods: Out of 20 potential markers of ketosis identified by in-silico analysis, two were selected and analyzed by qRT-PCR technique (upregulated; acetyl serotonin o-methyl transferase like and down regulated; guanylate cyclase activator 1B). Additional two sets of genes (carnitine palmotyl transferase A; upregulated and Insulin growth factor; downregulated) that have a role of hepatic fatty acid oxidation to maintain energy demands via gluconeogenesis were also validated. Extracted cDNA (complementary deoxyribonucleic acid) from the blood of the buffaloes were used for validation of selected genes via qRTPCR. Concentrations of BHBA, glucose and oxidative stress markers were identified with their respective optimized protocols. Results: The analysis of qRT-PCR gave similar trends as shown by in-silico analysis throughout the transition period. Significant changes (p<0.05) in the levels of BHBA, glucose and oxidative stress markers throughout this period were observed. This study provides validation from in-silico and qRT-PCR assays for potential markers to be used for earliest diagnosis of negative energy balance in buffaloes. Conclusion: Apart from conventional diagnostic methods, this study improves the understanding of putative biomarkers at the molecular level which helps to unfold their role in normal immune function, fat synthesis/metabolism and oxidative stress pathways. Therefore, provides an opportunity to discover more accurate and sensitive diagnostic aids.