• Title/Summary/Keyword: E.coil

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Development of Transcutaneous Energy Transmission System for Implantable Devices (생체 이식형 무선에너지 전송 시스템 개발)

  • Yoo Dong-Soo;Lee Joon-Ha;Seo Hee-Don;Lee Sang-Hag
    • Progress in Medical Physics
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    • v.16 no.3
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    • pp.155-159
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    • 2005
  • As a part of implantable device in body, a transcutaneous energy transmission system has been developed. It would be desirable to tansfer electrical energy to implantable devices transcutaneously. The distance between transcutaneous transformer windings are approximately equal to the thickness of the human's skin, nominally between 10$\~$20 mm. Class-E resonant amplifier is used to drive a primary coil for high efficiency. Maximum current is above 50 mA at any frequency. The developed system shows that the circuit operates correctly at each frequency; 500 kHz, 1 MHz and 4 MHz.

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Intergeneric Transfer of Nitrogen Fixation Genes from Rhizobium leguminosarum by RP4::Mu cts (RP4::Mu cts에 의한 Rhizobium leguminosarum 질소고정 유전자의 속간전달에 관한 연구)

  • 허연주;이영록
    • Korean Journal of Microbiology
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    • v.24 no.3
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    • pp.211-220
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    • 1986
  • Nitrogen fixation (nif) genes of Rhizobium leguminosarum were transferred to nif Klebsiella pneumoniae and E. coli by conjugation after partial heat induction of $RP_4$ :: Mu cts in Rhizobium $R^+$ transconjugant, and the hybrid plasmids in the transconjugant strains were isolated and characterized. In order to transfer the nif genes from Rhizobium, the hybrid plasmid $RP_4$ :: Mu cts was transferred by conjugation from E. coil to the symbiotic nitrogen fixer, R. leguminosarum. After stabillity test, the $RP_4$ :: Mu cts in Rhixobium $R^+$ transconjugant was subjected to partial heat induction by culturing it statically at $38^{\circ}C$ for 16 hours, and then conjugated with the nif defective mutant strains of K. pneumoniae or nif mutant strains of E. coli having whole nif gene plasmid. Recombinant strains of K. pneumoniae, which could grow in a N-free medium and exhibit the nitrogenase activity were selected. However, in the case of E. coli, they could grow well in a NA medium containing antibiotices, but hardly frow in a N-free medium. The hybrid plasmids in these transconjugal strains were isolated by gel electrophoresis and compared their molecular sizes.

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Plasma Charge Damage on Wafer Edge Transistor in Dry Etch Process (Dry Etch 공정에 의한 Wafer Edge Plasma Damage 개선 연구)

  • Han, Won-Man;Kim, Jae-Pil;Ru, Tae-Kwan;Kim, Chung-Howan;Bae, Kyong-Sung;Roh, Yong-Han
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2007.06a
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    • pp.109-110
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    • 2007
  • Plasma etching process에서 magnetic field 영향에 관한 연구이다. High level dry etch process를 위해서는 high density plasma(HDP)가 요구된다. HDP를 위해서 MERIE(Magnetical enhancement reactive ion etcher) type의 설비가 사용되며 process chamber side에 4개의 magnetic coil을 사용한다. 이런 magnetic factor가 특히 wafer edge부문에 plasma charging에 의한 damage를 유발시키고 이로 인해 device Vth(Threshold voltage)가 shift 되면서 제품의 program 동작 문제의 원인이 되는 것을 발견하였다. 이번 연구에서 magnetic field와 관련된 plasma charge damage를 확인하고 damage free한 공정조건을 확보하게 되었다.

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Antibacterial Activity and Identification of the Active Compound from Tumeric Extract (울금색소 추출물의 항균성과 물질분리 동정)

  • 한신영;최석철
    • Textile Coloration and Finishing
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    • v.14 no.1
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    • pp.11-17
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    • 2002
  • To identify the antimicrobial activity, of tumeric and its active compound tumeric was fractionated into four groups dichloromethane extract, hexane fraction, methanol soluble fraction and residue's extract. They were tested for antibacterial activity against E. coil and S. aureus and the methanol soluble fraction was found lo be the most active fraction. Compound I, II and III were isolated from TLC and silica gel column chromatography in the methanol soluble fraction. These compounds were analyzed by $^1H-NMR\;and\;^{13}C-NMR$ spectra and identified as curcumin I, II and III. They were also tested for antimicrobial activity against E. coli and S. aureus. Curcumin I was the must active curcuminoids due to the phenolic and methoxyl$(OCH_3)$ moiety in the same molecular structure.

Diversity and Function of Retinal-binding Protein in Photosynthetic Microbes

  • Jung, Kwang-Hwan
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2005.05a
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    • pp.64-66
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    • 2005
  • Photosynthetic microbes possess a wealth of photoactive proteins including chlorophyll-based pigments, phototropin-related blue light receptors, phytochromes, and cryptochromes. Surprisingly, recent genome sequencing projects discovered additional photoactive proteins, retinal-based rhodopsins, in cyanobacterial and algal genera. Most of these newly found rhodopsin genes and retinal synthase have not been expressed and their functions are unknown. Analysis of the Anabaena and Chlamyrhodopsin with retinal synthase revealed that they have sensory functions, which, based on our work with haloarchaeal rhodopsins, may use a variety of signaling mechanisms. Anabaena rhodopsin is believed to be sensory, shown to interact with a soluble transducer and the putative function is either chromatic adaptation or circadian rhythm. Chlamydomonas rhodopsins are involved in phototaxis and photophobic responses based on electrical measurements by RNAi experiment. In order to analyze the protein, we developed a sensory rhodopsin expression system in E. coli. The opsin in E. coil bound endogenous all-trans retinal to form a pigment and can be observed on the plate. Using this system we could identify retinal synthase in Anabaena PCC 7120. We conclude that Anabaena D475 dioxygenase functions as a retinal synthase to the Anabaena rhodopsin in the cell.

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Measurement and Control of the Resonance Frequency for the Transcutaneous Energy Transmission System (TET) Using the Phase Locked Loop Circuit (PLL) (PLL을 이용한 무선 전력전송 장치의 공진 주파수의 계측 및 주파수 제어)

  • Choi, S.W.;Shim, E.B.
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.1613-1616
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    • 2008
  • A Transcutaneous Energy Transmission System (TET) has been developed for the wireless energy transmission with two magnetically coupled coils. A resonance circuit is used to raise the induced voltage and current of the secondary coil. Its resonance frequency depends on the internal resistance of circuit and the transferred energy. Because the transferred energy usually changes in wide range, the output voltage is unstable and the energy transferring efficiency decrease. A push-pull class E amplifier is usedto generate high frequency AC voltage. To maintain proper resonance frequency, the voltage output of the amplifier was continuously monitored and adjusted to the optimized resonance frequency. Because of its high frequency (370 kHz), a phase lockedloop circuit and a comparator are used to monitor the output waveform. The results of experimentaldata show that the PLL circuit can increase the transmission efficiency and stabilize the output voltage of TET.

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Structural Characteristics of Regenerated Antheraea pernyi Silk Fibroin Film treated with ethanol (에탄올처리 재생 작잠 견피브로인 필름의 구조 특성)

  • 우순옥;권해용;엄인철;박영환
    • Journal of Sericultural and Entomological Science
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    • v.42 no.2
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    • pp.114-119
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    • 2000
  • Effects of ethanlo treatment on the structural and thermal characteristics of regenerated Antheraea pernyi silk fibroin (RSF) were investigated. Infrared spectroscopy and X-ray diffractometry showed that the conformational transition of RSF might be affected by concentration of ethanol and its treatment time. The structure of RSF was rapidly changed from random coil to $\beta$-sheet conformation when RSF was treated with les than 75% ethanol concentration. However, RSF treated with ethanol(100%) did not show conformational change. Differential scanning calorimetry showed that exotherm at 232$\^{C}$ disappeared and the intensity of endotherm at 228$\^{C}$ decreased with treatment of 75% ethanol. Dynamic thermal analysis showed that loss modulus (E") and tan $\delta$$\_$E/ of RSF treated with aqueous ethanol was broaden and shifted to higher temperature in comparison with those of untreated RSF.

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Comparative analysis of the minimum capacity of an ice-on-coil thermal storage system for various operation strategies (관외 빙착형 빙축열시스템의 운전방식에 따른 최소용량 비교)

  • 이대영;정성훈;강병하
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.11 no.3
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    • pp.401-413
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    • 1999
  • An ice storage cooling facility with cooling capacity of 150㎾ has been constructed for the purpose of developing optimal design and control strategy for an ice storage system. As the first step to this purpose, a computer program has been developed to simulate the operation of the ice storage system and examined precisely by comparing the results with those measured from the test facility. With the simulation program verified from the comparison, a design procedure has been developed to determine the minimum capacity required for each operation strategy available commercially. It is shown that the minimum sizes of the chiller and the storage tank are strongly dependent on the control strategy, i.e., chiller priority or storage priority, but less affected by the arrangement method, i.e., chiller upstream or chiller downstream.

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Antimicrobial Finish of Nonwoven Fabric by Treatment with Chitosan (키토산을 이용한 부직포의 항미생물가공)

  • Younsook Shin;Kyunghye Min
    • Textile Coloration and Finishing
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    • v.10 no.3
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    • pp.50-56
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    • 1998
  • Nonwoven fabric was treated with chitosan solution to impart antimicrobial activities by pad-dry method. Antimicrobial activity was measured by Shake Flask Method. Two chitosans of different molecular weight(Mw) with similar degree of deacetylation(DDA) were used : ca. 1,800(chitosan oligomer : DDA 84%) and 180,000(DDA 86%). Chitosan oligomer displayed high antimicrobial activity against P. vulgaris at 0.01%, S. aureus and E. coli at 0.05% treatment concentration, shelving above 90% of reduction rate. Chitosan of Mw 180,000 was effective against S. aureus, E. coil and P. vulgaris at 0.05% treatment concentration, showing almost 100% reduction rate. While chitosan of Mw 180,000 shelved reduction rate above 75% against K. pneumoniae and P. aeruginosa at 0.5% treatment concentration, chitosan oligomer was not effective against them. Fabrics become stiffer and less air permeable as treatment concentration increases. Liquid strike-through time of the sample treated with 0.5% chitosan oligomer solution (3.0 sec) was comparable with a hydrophilic finished sample commercially available(2.6 sec).

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A 6.78 MHz Constant Current and Constant Voltage Wireless Charger for E-mobility Applications (E-모빌리티 응용을 위한 6.78MHz 정전압 정전류 무선 충전기)

  • Tran, Manh Tuan;Choi, Woojin
    • Proceedings of the KIPE Conference
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    • 2019.07a
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    • pp.142-144
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    • 2019
  • Nowadays, multi-MHz wireless power transfer (WPT) system has received a great concern of study due to its desirable characteristics such as user convenience, system compact and better safety as compared to the conventional DC-DC with cord. This paper presents a solution for WPT Lithium Batteries charger with Constant Current (CC) and Constant Voltage (CV) charging process. The proposed system consists of a high frequency class D power amplifier, a pair of PCB coil, transformable high-order resonant network and a full-bridge rectifier. The charger can be implemented CC /CV charging profile thanks to automatic reconfigurable resonant compensator. Therefore, the battery can be fully charged without the help of an additional DC/DC converter. The simulation and 50W-6.78-MHz hardware experimental results are presented to verify the feasibility of the proposed method and to evaluate the performance of the proposed wireless battery charger.

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