• Title/Summary/Keyword: E. clavipes

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Notes on the genus Entoloma of Korea ( IX ) (한국산 외대버섯 속의 기록 ( IX ))

  • DuckHyunCho
    • Korean Journal of Plant Resources
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    • v.7 no.1
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    • pp.67-72
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    • 1994
  • Many species of the genus Entolama were collected at Mt. Jiri National Park, Mt. Baikyang National Park, Mt. Sunun Provincial Park and Mt. Balwang from June, 1991 to september, 1991.These sepecies were identified. According to the results, Entolama convexum, E. roanense, E.clavipes,E.tephreum, E.ochraceumand E.weberi were newly recorded in Korea.These species were described and their Korean names were designed.

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First report of Gymnosporangium clavipes Cooke & Peck affecting Crataegus mexicana var. Chapeado and C. gracilior in Mexico

  • Alvarado-Rosales, D.;Nieto-Lopez, E.H.;Teliz-Ortiz, D.;Ayala-Escobar, V.;Silva-Rojas, H.V.;Nieto-Angel, R.;Leyva-Mir, S.G.;Jimenez-Nieto, A.;Mendez-Inocencio, C.
    • Research in Plant Disease
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    • v.21 no.3
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    • pp.250-252
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    • 2015
  • The tejocote (Crataegus spp.) is a tree considered to be native to Mexico. The aim of this study was to identify the causal agent of tejocote rust in the State of Puebla. Tejocote fruits were sampled in 2012 and 2013. The fungus was studied morphologically using light and scanning electron microscopy and molecularly using phylogenetic analysis of 18S and 28S rDNA genes. The fungus was identified as Gymnosporangium clavipes on tejocote fruits. To our knowledge, this is the first confirmed report of Gymnosporangium clavipes Cooke & Peck affecting Crataegus mexicana var. Chapeado and C. gracilior in Puebla Mexico.

High-Level Production of Spider Silk Protein by Fed-Batch Cultivation of Recombinant Escherichia coli and Its Purification

  • Lee, Seok-Jae;Lee, Sang-Yeop
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.719-722
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    • 2001
  • Silk proteins from Nephila clavipes are fibrous proteins containing repetitive sequences with both crystalline and amorphous domains. In order to obtain high-level production of silk protein, the synthetic genes had 16 contiguous units of the consensus repeat sequence of the silk protein were expressed in Escherichia coli BL21(DE3) under the strong inducible T7 promoter. For production of recombinant silk protein in large amounts, pH-stat fed-batch cultures were carried out. The recombinant silk protein was produced as soluble forms in E. coli, and the recombinant silk protein content was as high as 11% of the total protein. When cells were induced at $OD_{600}$ of 60, the amount of silk protein produced was 6.49 g/L. After simple purification steps, 9.2 mg of silk protein that was more than 80% pure was obtained from a 50 mL culture, and the recovery yield was 26.3%.

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Inhibitory Activities of Basidiomycetes on Prolyl Endopeptidase, Acetylcholine Esterase and Coagulation (담자균 추출물의 Prolyl Endopeptidase, Acetylcholine Esterase 저해 및 항혈전 응고활성)

  • Lee, Hyun-Jin;Kim, Jong-Sik;Heo, Gun-Young;Lee, Kyung-Bok;Rhee, In-Koo;Song, Kyung-Sik
    • Applied Biological Chemistry
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    • v.42 no.4
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    • pp.336-343
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    • 1999
  • Fifty six mycelial cultured Basidiomycetes were screened for their inhibitory effects against prolyl endopeptidase(PEP), acetylcholine esterase(AChE) and thrombus coagulation. Out of them, methanolic extract of mycelium and/or ethylacetate(EtOAc) soluble fraction from culture broth of Peniophora quercina, Amanita aspera, Phellinus chrysoloma, Grifola frondosa, Wolfiporia extensa, Clavicorona pyxidata and Phanerochaete sordida inhibited more than 90% of PEP activity at 40 ppm. The extracts of Lenzites betulina, Phellinus chrysoloma, Wolfiporia extensa, Phanerochaete sorrlida, Hypocrea nigricans, Coriolus azureus, Flammulina velutipes, Phlebiopsis gigantea and Bondarzewia montana exhibited about 40% of inhibitory activity against AChE at 40 ppm. In thrombin times assay, the extracts of Amanita aspera, Oxyporus latemarginata, Peniophora quercina, Fomes fomenfarius, Trametes versicolor, and Phlebiopsis gigantea delayed coagulation of thrombus about two to three times over control at ca 550 ppm. In activated partial thromboplastin times assay, none of the tested Basidiomycetes showed significant effect.

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