• 한국자원식물학회지
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• 제7권1호
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• pp.67-72
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• 1994

Many species of the genus Entolama were collected at Mt. Jiri National Park, Mt. Baikyang National Park, Mt. Sunun Provincial Park and Mt. Balwang from June, 1991 to september, 1991.These sepecies were identified. According to the results, Entolama convexum, E. roanense, E.clavipes,E.tephreum, E.ochraceumand E.weberi were newly recorded in Korea.These species were described and their Korean names were designed.

• 식물병연구
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• 제21권3호
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• pp.250-252
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• 2015

The tejocote (Crataegus spp.) is a tree considered to be native to Mexico. The aim of this study was to identify the causal agent of tejocote rust in the State of Puebla. Tejocote fruits were sampled in 2012 and 2013. The fungus was studied morphologically using light and scanning electron microscopy and molecularly using phylogenetic analysis of 18S and 28S rDNA genes. The fungus was identified as Gymnosporangium clavipes on tejocote fruits. To our knowledge, this is the first confirmed report of Gymnosporangium clavipes Cooke & Peck affecting Crataegus mexicana var. Chapeado and C. gracilior in Puebla Mexico.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.719-722
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• 2001

Silk proteins from Nephila clavipes are fibrous proteins containing repetitive sequences with both crystalline and amorphous domains. In order to obtain high-level production of silk protein, the synthetic genes had 16 contiguous units of the consensus repeat sequence of the silk protein were expressed in Escherichia coli BL21(DE3) under the strong inducible T7 promoter. For production of recombinant silk protein in large amounts, pH-stat fed-batch cultures were carried out. The recombinant silk protein was produced as soluble forms in E. coli, and the recombinant silk protein content was as high as 11% of the total protein. When cells were induced at $OD_{600}$ of 60, the amount of silk protein produced was 6.49 g/L. After simple purification steps, 9.2 mg of silk protein that was more than 80% pure was obtained from a 50 mL culture, and the recovery yield was 26.3%.

• Applied Biological Chemistry
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• 제42권4호
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• pp.336-343
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• 1999

담자균류로부터 치매 예방 및 치료 효과를 갖는 생물활성물질을 탐색하기 위한 기초 자료를 확립하기 위하여 56종의 배양 균사체 메탄을 추출물 및 배양 여액의 ethylacetate(EtOAc) 가용성 분획에 대하여 prolylendopeptidase(PEP), acetylcholine esterase(AChE) 및 혈전 응고에 대한 저해활성을 측정하였다. 그 결과 PEP에 대하여 Amanita aspera, Phellinus chrysoloma의 균사체 추출물 및 배양여액의 EtOAc 가용성 분획이 40ppm에서 모두 90% 이상의 저해활성을 나타내었으며 분홍껍질고약버섯(Peniophora quercina), 잎새버섯(Grifola frondosa), Wolfiporia extensa, 좀나무싸리버섯(Clavicorona pyxidata) 및 Phanerochaete soy교교의 배양액이 90% 이상의 저해활성을 나타내었다. AChE에 대하여는 40ppm 농도에서 어느 것도 강한 활성을 나타내지 못하였으나 조개껍질버섯(Lenzites betulina), Phellinus chrysoloma, Wolfiporia extensa, Phanerochaete sordida의 균사체 추출물과 Hypocrea nigricans, Coriolus azureus, 팽나무버섯(Flammuzina velutipes), Phlebiopsis gigantea 및 Bondarzewia montana의 배양여액 EtOAc 가용성 분획이 40% 정도의 저해활성을 나타내었다. 한편 혈전응고 저해활성의 지표로 삼은 thrombin times(TT) assay에서는 Amanita aspera, Oxyporus latemarginata, 분홍껍질고약버섯(Peniophora quercina), 말굽버섯(Fomes fomefarius)의 배양여액 EtOAc 추출물과 Clitocybe clavipes, Trametes versicolor, Phlebiopsis gigantea의 균사체 추출물이 550 ppn의 농도에서 혈전 생성에 걸리는 시간을 2배 내지 3배 연장하는 효과를 나타내었으나 activated partial thromplastin times(APTT) assay에서는 어느 것도 효과를 인정할 수 없었다.