• Journal of Microbiology and Biotechnology
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• v.25 no.9
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• pp.1467-1475
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• 2015

The use of microbial extracts containing plant hormones is a promising technique to improve crop growth. Little is known about the effect of bacterial cell-free extracts on plant growth promotion. This study, based on phytohormonal analyses, aimed at exploring the potential mechanisms by which Enterococcus faecium LKE12 enhances plant growth in oriental melon. A bacterial strain, LKE12, was isolated from soil, and further identified as E. faecium by 16S rDNA sequencing and phylogenetic analysis. The plant growth-promoting ability of an LKE12 bacterial culture was tested in a gibberellin (GA)-deficient rice dwarf mutant (waito-C) and a normal GA biosynthesis rice cultivar (Hwayongbyeo). E. faecium LKE12 significantly improved the length and biomass of rice shoots in both normal and dwarf cultivars through the secretion of an array of gibberellins (GA₁, GA₃, GA₇, GA₈, GA₉, GA₁₂, GA₁₉, GA₂₀, GA₂₄, and GA₅₃), as well as indole-3-acetic acid (IAA). To the best of our knowledge, this is the first study indicating that E. faecium can produce GAs. Increases in shoot and root lengths, plant fresh weight, and chlorophyll content promoted by E. faecium LKE12 and its cell-free extract inoculated in oriental melon plants revealed a favorable interaction of E. faecium LKE12 with plants. Higher plant growth rates and nutrient contents of magnesium, calcium, sodium, iron, manganese, silicon, zinc, and nitrogen were found in cell-free extract-treated plants than in control plants. The results of the current study suggest that E. faecium LKE12 promotes plant growth by producing GAs and IAA; interestingly, the exogenous application of its cell-free culture extract can be a potential strategy to accelerate plant growth.

• Korean Journal of Food Science and Technology
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• v.38 no.5
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• pp.668-673
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• 2006

The effects of Acanthopanacis cortex water extract on allergic contact dermatitis (ACD) in Sprague-Dawley male rats treated with 2.5% 1-chloro-2,4-dinitrochlorobenzene (DNCB) on the dorsal skin were evaluated. The concentration of TBARS in plasma and liver was increased in ACD rats and was significantly decreased in Acanthopanacis cortex fed group (ACFG) compared to CG (control group). Serum Ig E level was significantly increased in CG compared to normal group, while that of ACFG was significantly decreased. The epidermal thickness of CG was significantly increased compared to that of normal group, while that of ACFG was significantly decreased compared to that of CG These results indicated that the Acanthopanacis cortex water extract administration improved lipid preoxidation, antioxidative activity, serum Ig E level and epidermal thickness in rats with ACD.

• Nutrition Research and Practice
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• v.1 no.3
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• pp.184-188
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• 2007

The major goal in the treatment of diabetes mellitus is to achieve near-normal glycemic control. To optimize both fasting blood glucose and postprandial glucose levels is important in keeping blood glucose levels as close to normal as possible. ${\alpha}-Glucosidase$ is the enzyme that digests dietary carbohydrate, and inhibition of this enzyme could suppress postprandial hyperglycemia. The purpose of this study was to test the inhibitory activity of methanol extract of Euonymus alatus on ${\alpha}-glucosidase$ in vitro and in vivo to evaluate its possible use as an anti-diabetic agent. Yeast ${\alpha}-glucosidase$ inhibitory activities of methanol extract of E. alatus were measured at concentrations of 0.50, 0.25, 0.10, and 0.05 mg/ml. The ability of E. alatus to lower postprandial glucose was studied in streptozotocin-induced diabetic rats. A starch solution (1 g/kg) with and without E. alatus extract (500 mg/kg) was administered to diabetic rats by gastric intubation after an overnight fast. Plasma glucose levels were measured at 30, 60, 90, 120, 180, and 240 min. Plasma glucose levels were expressed in increments from baseline, and incremental areas under the response curve were calculated. Extract of E. alatus, which had an $IC_{50}$ value of 0.272 mg/ml, inhibited yeast ${\alpha}-glucosidase$ activity in a concentration-dependent manner. A single oral dose of E. alatus extract significantly inhibited increases in blood glucose levels at 60 and 90 min (p<0.05) and significantly decreased incremental response areas under the glycemic response curve (p<0.05). These results suggest that E. alatus has an antihyperglycemic effect by inhibiting ${\alpha}-glucosidase$ activity in this animal model of diabetes mellitus.

• Korean Journal of Agricultural and Forest Meteorology
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• v.19 no.3
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• pp.110-119
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• 2017

The agroclimatic indices are produced by statistical analysis based on primary climate data (e.g., temperature, precipitation, and solar irradiance) or driving agronomic models. This study was carried out to evaluate how selection of daily temperature for a climate normal (1983-2012) affected the precision of the agroclimatic indices. As a first step, averaged daily 0600 and 1500 LST temperature for a climate normal were produced by geospatial schemes based on topo-climatology ($365days{\times}1$ set, EST normal year). For comparison, 30 years daily temperature data were generated by applying the same process ($365days{\times}30sets$), and calculated mean of daily temperature (OBS normal year). The flowering date of apple 'Fuji' cultivar, the last frost date, and the risk of late frost were estimated based on EST normal year data and compared with the results from OBS normal year. The results on flowering date showed 2.9 days of error on average. The last frost date was of 11.4 days of error on average, which was relatively large. Additionally, the risk of the late frost was determined by the difference between the flowering and the last frost date. When it was determined based on the temperature of EST normal year, Akyang was classified as a risk area because the results showed that the last frost date would be the same or later than the flowering date in the 12.5% of area. However, the temperature of OBS normal year indicated that the area did not have the risk of a late frost. The results of this study implied that it would be necessary to reduce the error by replacing the EST method with the OBS method in the future.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.22 no.4
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• pp.279-285
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• 2010

In the process of the probabilistic-based design on the coastal structures, the probability density function (pdf) of tidal elevation data is assumed as the normal distribution function. The pdf shape of tidal elevation data, however, is better-fitted to the double-peak normal distribution function and the equivalent mean and standard deviation (SD) estimation process based on the equivalent normal distribution is required. The equivalent mean and SD (equivalent parameters) are different with the mean and SD (normal parameters) estimated in the condition that the pdf of tidal elevation is normal distribution. In this study, the difference, i.e., estimation error, between equivalent parameters and normal parameters is compared and analysed. The difference is increased as the tidal elevation and its range are increased. The mean and SD differences in the condition of the tidal elevation is ${\pm}400cm$ are above 100 cm and about 80~100 cm, respectively, in Incheon station. Whereas, the mean and SD differences in the condition of the tidal elevation is ${\pm}60cm$ are very small values in the range of 2~4 cm, in Pohang station.

• The Journal of the Acoustical Society of Korea
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• v.17 no.2E
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• pp.47-52
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• 1998

An analysis of dynamic responses is carried out on monoclinic anisotropic system due to a buried harmonic line source. The load is in the form of a normal stress acting along an arbitrary axis on the plane of symmetry within the orthotropic materials: In case that the line load is acting along the symmetry axis normal to the plane of symmetry, plane wave equation is coupled with verital shear wave and longitudinal wave. However, if the line load is acting along an arbitrary axis normal to the plane of symmetry, plane wave equation is coupled with vertical shear wave, longitudinal wave and horizontal shear wave. We first considered the equation of motion in a reference coordinate system, where the line load is coincident with a symmetry axis of the orthotropic material. Then the equation of motion is transformed into one with respect to general coordinate system with azimuthal angle by using transformation tensor. Plane wave solutions of monoclinic systems are derived for infinite media. Finally complete solutions for the plane harmonic wave are obtained by calculating the inverse of the integral transforms, in which bulk wave poles are avoided by deforming the contour of the integration to the complex plane. Numerical results for examples of orthotropic material belonging to monoclinic symmetry are demonstrated.

• Journal of the Korean earth science society
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• v.29 no.5
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• pp.437-445
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• 2008

We analyzed fault plane solutions of the recent twenty-two earthquakes which occurred from 2004 to 2006 in the central part of the Korean Peninsula by using P- and S-wave polarities along with SH/P amplitude ratios. The fault plane solution shows that strike-slip fault is dominant here, especially for the events with local magnitude equal to or greater than 3.0. However, some events with local magnitude less than 3.0 show normal fault or strike-slip fault with normal components. In the case of strike-slip fault, its orientation is almost in the direction of NNE-SSW to NE-SW almost parallel to the general trend of faults, while the compressional axis of the stress field trends ENE to E-W. The result is almost consistent with the stress field in and around the Korean peninsula, as reported previously. We cannot give any appropriate explanations to the normal faulting events along the western offshore and inland areas whether it is related to the local stress changes or tectonically unidentified extensional structures. Thus, an extension of investigations is desirable to clarify the cause of such phenomena.

• Korean Journal of Materials Research
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• v.21 no.2
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• pp.73-77
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• 2011

Specific heat of a crystal is the sum of electronic specific heat, which is the specific heat of conduction electrons, and lattice specific heat, which is the specific heat of the lattice. Since properties such as crystal structure and Debye temperature do not change even in the superconducting state, the lattice specific heat may remain unchanged between the normal and the superconducting state. The difference of specific heat between the normal and superconducting state may be caused only by the electronic specific heat difference between the normal and superconducting states. Critical temperature, at which transition occurs, becomes lower than $T_{c0}$ under the influence of a magnetic field. It is well known that specific heat also changes abruptly at this critical temperature, but magnetic field dependence of jump of specific heat has not yet been developed theoretically. In this paper, specific heat jump of superconducting crystals at low temperature is derived as an explicit function of applied magnetic field H by using the thermodynamic relations of A. C. Rose-Innes and E. H. Rhoderick. The derived specific heat jump is compared with experimental data for superconducting crystals of $MgCNi_3$, $LiTi_2O_4$ and $Nd_{0.5}Ca_{0.5}MnO_3$. Our specific heat jump function well explains the jump up or down phenomena of superconducting crystals.

• Proceedings of the Korea Concrete Institute Conference
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• 1994.10a
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• pp.356-361
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• 1994

From the previous experimental test results, it has shown that shear that shear strength in lightweight concrete beams was about 85% on that in normal concrete beams. It is speculated that shear connectors in composite beams of steel and lightweight concrete associated with the longitudinal shear strength decrease more in strength than those in normal concrete. So this paper, as a study on strength of shear connectors in composite beams of steel and lightweight concrete slabs, has a purpose to compare the strength formula resulted from the push-out test of thirteen solid slab and four deck Plate slab with the established ones, and then to suggest a proper strength formula of the shear connectors. The established strength formula of the shear connectors is prescribed for $P_ps = 0.50A_s . \sqrt{f_C . E_C}$by AISC coed, but from the experimental test results the strength values of the shear connectors in lightweignt concrete slabs shows about 70% on those of the shear connectors in normal concrete slabs by AISC code. Therefore, as a strength formula this paper suggests to multiply the established strength formula by reduction factor$(\varphi=0.7)$.

• Journal of Mushroom
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• v.15 no.4
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• pp.164-167
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• 2017

Biological efficiency (BE), the ratio of fresh mushrooms harvested per dry substrate weight, expressed as the percentage of Lentinula edodes, also known as shiitake, was determined using the 'Sanjo 701' strain stored in the Department of Mushroom at the Korea National College of Agriculture and Fisheries. The mycelia were grown in glass columns with varying levels of moisture content and varying mixing periods of 0.5, 1, 2, and 3 hours. The substrate was sterilized using a steam pressure autoclave sterilizer at normal and high pressure to avoid contamination. The results showed that mycelial growth (126 mm/15 days) was optimized at 55% moisture content. The best mycelial growth of 117 mm/15 days was obtained with 2 hours of mixing time. Normal pressure sterilization yielded better results with mycelial growth of 96 mm/15 days at $100^{\circ}C$ compared to 88 mm /15 days with sterilization at $121^{\circ}C$. Mycelial density was higher, i.e. 3(+++), with normal pressure sterilization compared to 2(++) with high pressure sterilization. Furthermore, sawdust mixed with 5% woodchips increased the substrate porosity and yielded higher mycelial growth. Thus, we demonstrated that the optimum harvest or potential increased yield of shiitake can be obtained by modulating moisture content, mixing time, and substrate porosity.