• Environmental Mutagens and Carcinogens
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• v.24 no.2
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• pp.85-90
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• 2004

Nickel(II) compounds are carcinogenic metals which induce genotoxicity and oxidative stress through the generation of reactive oxygen species. In search of new molecular pathways toward understanding the molecular mechanism of nickel(II)-induced carcinogensis, we performed mRNA differential display analysis using total RNA extracted from nickel(II) acetate-treated normal rat kidney cells (NRK-52E). Cells were exposed for 3 days to 160 and 240 uM nickel(II) concentrations. cDNAs corresponding to mRNAs for which expression levels were altered by nickel(II) were isolated, sequenced, and followed by a GenBank Blast homology search. Specificity of differential expression of cDNAs was determined by RT-PCR and Western blot analysis. Two of them (SH3BGRL3 and FHIT) were down-regulated and one (metallothionein) was up-regulated by nickel(II) treatment. The expression of these mRNAs were nickel(II) concentration-dependent. The levels of FHIT and metallothionein proteins were also consistent with the results for mRNAs. Overall, although the fundamental questions related to function of these genes in nickel(II)-mediated carcinogenicity are not answered, our study suggests that they can be interesting candidates for studies of molecular mechanisms of nickel(II) carcinogenesis.

• Journal of the Korean Society of Safety
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• v.22 no.5
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• pp.84-89
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• 2007

The lower explosion limit(LEL) is one of the major combustion properties used to determine the fire and explosion hazards of the combustible substances. In this study, the lower explosion limits of the ester compounds were predicted by using the normal boiling points and the flash points based on the liquid thermodynamic theory. As a results, the A.A.P.E.(average absolute percent error) and the A.A.D.(average absolute deviation) of the reported and the calculated the LEL for the ester are 8.80 vol% and 0.18 vol%, respectively and the coefficient of correlation was 0.965. From a given results, by the use of the proposed methodology, it is possible to predict the lower explosion limits of the other flammable materials.

• Journal of the Korean Graphic Arts Communication Society
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• v.29 no.2
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• pp.15-32
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• 2011

In printing, managing color means that how closely its color reproducts and printing supplier meets customers' requirements. When applying device profiles, it depends on properties of the devices. But color management of domestic digital prints is accomplished more scientifically and objectively than any other printing. According to this paper addresses a method of evaluating between proof prints and offset prints which are produced by identical date on the field. We evaluate two cases normal proof prints and domestic offset prints based on standardized color data analysis and subjective data analysis. We gathered objective data by measuring solid density, $CIEL^*a^*b^*$ and ${\Delta}E^{*_}{ab}$. Furthermore, we evaluated the offset prints and proof prints through human eyes to decide the ranking.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.8
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• pp.1088-1092
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• 2004

The present investigation was undertaken to study the status of plasma antioxidant vitamins in normal cycling and $\alpha$-tocopherol supplemented anestrus buffalo heifers. The pre-supplementation plasma levels ($\mu$mol/L) of vitamin E and $\beta$-carotene were significantly (p<0.05) lower and of vitamin C was significantly (p<0.05) higher in anestrus heifers (4.06$\pm$0.07; 4.56$\pm$0.17; 21.04$\pm$0.21) when compared to normal cycling ones (4.92$\pm$0.05; 6.76$\pm$0.12; 14.24$\pm$0.16). The oral supplementation of$\alpha$-tocopherol at 3,000 mg per week per animal in anestrus heifers resulted in a significant (p<0.01) increase in vitamin E and $\beta$-carotene levels and a significant (p<0.01) decrease in vitamin C concentration. Results indicated that supplementation of $\alpha$-tocopherol to anestrus buffalo heifers improved the antioxidant status by mitigating the harmful effects of free radical induced oxidative stress.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.85-96
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• 2003

Normal human skin, constantly challenged by environmental micro-organisms, has an innate ability to fight invading microbes through antimicrobial peptides. These peptides, described in both plant and animal kingdoms are able to inactivate a broad spectrum of micro-organisms. Mammalian defensins constitute one of the most common antimicrobial peptide family. Among the three human beta-defensins hBD1, hBD2 and hBD3 produced in epithelia, only hBD2 and hBD3 are inducible and additionally have been described as expressed by differentiated keratinocytes at site of inflammation and infection. The aims of these studies were to define a cell culture model in which the basal production of hBD could be detected and up-regulated in order to enhance skin auto-protection against micro-organisms. A specific Polymerase Chain Reaction method have been developed for hBD2 and hBD3 mRNA detection in non-differentiated monolayer keratinocytes cell culture. We have been able to demonstrate that in vitro, hBD2 and hBD3 expression in normal human keratinocytes could be detected and enhanced by TNF-alpha and IFN-gamma, in hypercalcic culture conditions. This research opened the possibility of the development of cosmetic active compounds, able to induce the expression of skin natural antibiotic peptides responsible about microflora ecology of the skin.

• Korean Journal of Biological Psychiatry
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• v.19 no.1
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• pp.9-16
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• 2012

This article aimed to describe typical event-related potentials (ERP) components of emotional processing in patients with anxiety disorder and highly anxious individuals. ERP components associated with emotional processing could be broadly divided into three components with short, middle and long, respectively. Many studies show that patients with anxiety disorders are characterized by different emotional bias to specific stimuli and more sensitive to emotional stimuli than normal individuals. In addition, these emotional biases were stronger and quicker in patients with anxiety disorder than normal individuals. Some studies reported that anxious people show abnormality at the initial stage (e.g. P1) of emotional processing. However, other studies reported the abnormality at the late stage (e.g. LPP) or wholeness of emotional processing in anxious individuals. We summarized the updated finding of possible ERP components of emotional processing in patients with anxiety disorder and highly anxious individuals. The significance and clinical implication were discussed.

• Phonetics and Speech Sciences
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• v.6 no.3
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• pp.3-12
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• 2014

Larynx plays an important role in phonation and protection of the respiratory tract during swallowing. The reduced anatomical and physiological function in elevation of larynx and glottis closure can cause problems in voice and swallowing. The present study investigated the Korean version of handicap index of dysphagia in elderly Koreans. Therefore, 60 normal elderly Koreans ranged from 65 to 95 and 20 normal Korean young adults aged from 20 to 25 were participated in this study to compare total (T), physical (P), functional (F), and emotional (E) index scores between two groups as well as among sub groups (60s, 70s, 80s) in elderly. For swallowing, total and sub dysphagia handicap index (DHI) scores, voice quality during /a/phonation following swallowing (saliva and water), intensity of coughing, and L-DDK were measured. The results showed that functional (P), physical (P), emotional (E) scores as well as total (T) score were significantly different between young adults and old adults in DHI(p<.05). Additionally, there was a negative correlation between total DHI score and intensity of coughing (r=-.51) as well as L-DDK (r=-.70). These findings suggest that a slow rate in vocal fold adduction and reduced intensity of coughing in the elderly affect swallowing function. Thus, recently translated Korean version of DHI may be useful as supplement in evaluating the swallowing problems in elderly people.

• Biomolecules & Therapeutics
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• v.22 no.1
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• pp.47-54
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• 2014

The present study was aimed to evaluate the antioxidant defense system of cinnamaldehyde in normal, diabetic rats and its possible protection of pancreatic ${\beta}$-cells against its gradual loss under diabetic conditions. In vitro free radical scavenging effect of cinnamaldehyde was determined using DPPH (1,1-diphenyl-2-dipicrylhydrazyl), superoxide radical, and nitric oxide radical. Streptozotocin (STZ) diabetic rats were orally administered with cinnamaldehyde at concentrations of 5, 10 and 20 mg/kg body weight for 45 days. At the end of the experiment, the levels of plasma lipid peroxides and antioxidants such as vitamin C, vitamin E, ceruloplasmin, catalase, superoxide dismutase, reduced glutathione and glutathione peroxidase were determined. A significant increase in the levels of plasma glucose, vitamin E, ceruloplasmin, and lipid peroxides and significant decrease in the levels of plasma insulin and reduced glutathione were observed in the diabetic rats. Also the activities of pancreatic antioxidant enzymes were altered in the STZ-induced diabetic rats. The altered enzyme activities were reverted to near-normal levels after treatment with cinnamaldehyde and glibenclamide. Histopathological studies also revealed a protective effect of cinnamaldehyde on pancreatic ${\beta}$-cells. Cinnamaldehyde enhances the antioxidant defense against reactive oxygen species produced under hyperglycemic conditions and thus protects pancreatic ${\beta}$-cells against their loss and exhibits antidiabetic properties.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.13 no.1
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• pp.40-44
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• 2002

Background and Objectives : Vocal abuse and misuse and muscle tension dysphonia that have various movements of false vocal folds may be related to the development of benign vocal folds lesions, such as vocal nodules, polyps, and cysts. This study was designed to determine whether benign vocal folds lesions were related with movements of false vocal folds on phonation. Material and Methods : One Hundred and seventy eight subjects were studied. All subjects received otolaryngological evaluation including videostroboscopy, objective voice measures. Patients were diagnosed as normal shape of vocal folds (group a), approximation of bilateral false vocal folds (group b), approximation of unilateral false vocal folds (group c), lateralized extension of false vocal folds (group d), and medialized approximation of posterior false vocal folds (group e). We analyzed the results of benign vocal folds lesions in each group. Results : Differences were found between the normal shaped group and the abnormal shaped group. No differences were found between each abnormal groups except group d and e. Conclusion : The shape of false vocal folds was related to the benign vocal folds lesions.

• Restorative Dentistry and Endodontics
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• v.18 no.2
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• pp.377-386
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• 1993

The purpose of this study was to evaluate the disinfection efficacy of root canal irrigation method and irrigation solution in infected root canals. Access cavity were prepared in single-rooted extracted human teeth and each canals were prepared with step-back method and sterilized with ethylene oxide gas. Prepared teeth were experimentally inoculated in the canals for 30 minutes with four aerobes(Streptococcus mutans, Stapylococcus aures, Streptococcus sanguis, and E. coli) and two anaerobes(Streptoccus intermedius, and Clostrium perfringens). Normal saline, 3% $H_2O_2$, and 3% NaOCl were used as irrgation solutions. And root canals were irrigated with each irrigation solutions manually with disposible syringe or ultrasonic instument. (Apoza Uitrasonc, Taiwan). The irrigation effect on the eradication of S. aures and S. intermedius with normal saline was not similar between manual method and ultrasonic method. No significant differences were observed in the eradication of E. coli and C. perfringens among the irrigation solution and irrespective of the irrigation methods. In S. aurues and S. mutans, there was somewhat different eradication effect according to irrigation solutions, but there was no significant difference between manual method and ultrasonic methods. These results suggest that ultrasonic irrigation method with saline is more effective in some strains than manual irrigation.