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The Effect of Hongdeung-tang on Endoxotin-induced Disseminated Intravascular Coagulation (홍등탕(紅藤湯)이 내독소로 유발된 파종성혈관내응고증에 미치는 영향)

  • Lim, Eun-Mee;Jo, Young-Rai
    • The Journal of Korean Obstetrics and Gynecology
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    • v.19 no.1
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    • pp.139-154
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    • 2006
  • Objective : This experimental study was carried out to investigate anti-coagulation effects and mechanism of Hongdeung-tang on the dissentimenated intravascular coagulation Methods : Each 10 rats were classified as normal gorup, control group and treated group. The control group was given a 0.5mg/ml endotoxin intra-vein injection and was occurred Disseminated Intravascular Coagulation(DIC). The treated group were orally administrated 1ml Hongdeung-tang concentration once a day for 6 days and then it was treated the same as the control group. We took the blood from heart and were investigated number of Platelet and white blood cell(WBC), fibrinogen concentration, prothrombin time(PT), activated partial thromboplastin time(APTT), interleukin-6(IL-6), Tumor Necrosis $Factor-{\alpha}(TNF-{\alpha})$, Prostaglandin $E2(PGE_2)$ from them. Results : There were a statistically significant increase of platelet and fibrinogen concentration in the treated group compared to the treated group. The PT was a significantly reduced in treated group compared to the control group. There was no significant change in numbers of WBC and APTT between control and treated group. On the contrary, the number of PT, IL-6, $TNF-{\alpha}$, $PGE_2$ were significantly decreased in treated group compared to the control group. Conclusion : DIC which was occurred by endotoxin intravenous injection seems to related to the increase of IL-6, $TNF-{\alpha}$, $PGE_2$ and Hongdeung-tang has an effect that supress those factors. we can assume Hongdeung-tang has an anti-coagulation effect by those supressing effect.

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Identification and Characterization of a RecA-like Protein Induced by DNA Damaging Agents in Fluorescent Pseudomonas sp.

  • Kim, Ok-Bong;Lim, Chae-Kwang;Kim, Si-Wouk;Park, Jong-Kun;Yoon, Seong-Myeong;Lee, Jung-Sup
    • Animal cells and systems
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    • v.2 no.3
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    • pp.383-388
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    • 1998
  • A RecA-like protein (RecAps) was identified from fluorescent Pseudomonas sp. and the inducible nature of the protein was characterized in detail. It was shown by dose-response and time-course experiments using two DNA damaging agents, nalidixic acid and mitomycin-C, that the cellular level of RecAps protein was increased 3-8 fold compared to that of the control. The most effective doses of nalidixic acid and mitomycin-C for the protein induction were $30{\mu}g/ml$ and $0.3{\mu}g/ml$ at the treatment time point of 150 min, respectively. The enhanced level of RecAps protein was gradually decreased to the control level after 10 hr in normal medium. Interestingly, the cellular level of RecAps protein was increased by the same DNA damaging agents even when cell growth was completely inhibited by treatment with $170{\mu}g/ml$ of chloramphenicol, an inhibitor of protein synthesis, suggesting that new protein synthesis is not required for the induction of RecAps. All these results suggest that a typical S0S repair function driven by RecA-like protein is conserved in Pseudomonas sp. cells as in E, coli.

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Dependence of Magnetic and Magneto-Optic Properties on Deposition Angle in E-Beam EVaporated Co/Pt Multilayer Films (전자빔 증착 Co/Pt 다층박막에서 입사 선속의 방향에 따른 자기 및 자기광학적 성질 변화 연구)

  • 문기석;신성철
    • Journal of the Korean Magnetics Society
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    • v.4 no.4
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    • pp.313-318
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    • 1994
  • We have investigated the effects of deposition angle on magnetic and magneto-optic properties in Co/Pt multilayer thin films. which were prepared bye-beam evaporation on tilted substrates. with varying tilt angle from $0^{\circ}$ to $60^{\circ}$. The structure of the specimens was examined by x-ray diffractometer and scanning electron microscope. and the magnetic and magneto-optical properties were measured by VSM, torque magnetometer, and Kerr loop tracer. X-ray diffractometry revealed that all of the specimens had multilayer structure and growth orientation of column followed the tangent rule but the crystallograpic orientation, <111>, was slightly deviated from the substrate normal even though the deposition angle was increased up to $60^{\circ}$. A decrement of the magnetization and Kerr angle with the deposition angle was related with that of the film density due to increasing porosity. The perpendicular mag¬netic anisotropy was also decreased with increasing the deposition angle.

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The Effect of Plantaginis Semen Herbal Acupuncture on Acute Renal Failure in Rat (차전자약침(車前子藥鍼)이 급성신부전(急性腎不全) 백서(白鼠)에 미치는 영향(影響))

  • Cho, Si-Yong;Kim, Cheol-Hong;Youn, Hyoun-Min;Jang, Kyung-Jeon;Ahn, Chang-Beohm;Song, Choon-Ho
    • Korean Journal of Acupuncture
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    • v.22 no.4
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    • pp.117-127
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    • 2005
  • Objective : This study was undertaken to determine if Plantaginis Semen herbal acupuncture(PSA) has a protective effect on glycerol-induced acute renal failure in rats. Methods : Rats were dehydrated for 24hr and then injected with 4 ml/kg of 50% glycerol in each hindlimb muscle. In experiments for PSA effect, rats were received 0.1 ml of PSA extraction in both sides of corresponding $Sh\grave{e}nsh\bar{u}(BL_{23})$ of human body for 3 days after injection of glycerol. The experimental group were divided into the normal group, the control group, the sample 1,2,3 group. Results : Glycerol injection decreased glomerular filtration rate and increased urine volume, serum creatinine, BUN level and fractional excretion of $Na^+,\;K^+\;and\;Cl^-$. These results show that glycerol injection bring about acute renal failure. PSA significantly increased glomerular filtration rate and significantly decreased serum creatinine, BUN level and fractional excretion of $Na^+,\;and\;Cl^-$ as compared with control group. Conclusion : These results suggest that PSA can be used in prevention and treatment for acute renal failure. However, the precise mechanisms of PSA protection remain to be determined.

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Effects of Au Nanoparticle Monolayer on or Under Graphene for Surface Enhanced Raman Scattering

  • Kim, B.Y.;Jung, J.H.;Sohn, I.Y.;Lee, N.E.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.636-636
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    • 2013
  • Since first discovery of strong Raman spectrum of molecules adsorbed on rough noble metal, surface enhanced Raman scattering (SERS) has been widely used for detection of molecules with low concentration. Surface plasmons at noble metal can enhance Raman spectrum and using Au nanostructures as substrates of SERS has advantages due to it has chemical stability and biocompatibility. However, the photoluminescence (PL) background from Au remains a problem because of obtaining molecular vibration information. Recently, graphene, two-dimensional atomic layer of carbon atoms, is also well known as PL quenchers for electronic and vibrational excitation. In this study, we observed SERS of single layer graphene on or under monolayer of Au nanoparticles (NPs). Single layer graphene is grown by chemical vapor deposition and transferred onto or under the monolayer of Au NPs by using PMMA transfer method. Monolayer of Au NPs prepared using Langmuir-Blodgett method on or under graphene surface provides closed and well-packed monolayer of Au NPs. Scanning electron microscopy (SEM) and Raman spectroscopy (WItec, 532 nm) were performed in order to confirm effects of Au NPs on enhanced Raman spectrum. Highly enhanced Raman signal of graphene by Au NPs were observed due to many hot-spots at gap of closed well-packed Au NPs. The results showed that single layer graphene provides larger SERS effects compared to multilayer graphene and the enhancement of the G band was larger than that of 2D band. Moreover, we confirm the appearance of D band in this study that is not clear in normal Raman spectrum. In our study, D band appearance is ascribed to the SERS effect resulted from defects induced graphene on Au NPs. Monolayer film of Au NPs under the graphene provided more highly enhanced graphene Raman signal compared to that on the graphene. The Au NPs-graphene SERS substrate can be possibly applied to biochemical sensing applications requiring highly sensitive and selective assays.

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Practical Implications in the Setting Aside and the Refusal of Enforcement of Arbitral Award - Focusing on the Public Policy - (중재판정의 취소와 집행거부에 따른 실무상의 유의점 - 공서위반을 중심으로 -)

  • Oh, Won-Suk;Kim, Yong-Il
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.35
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    • pp.101-124
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    • 2007
  • This paper purposes to examine the setting aside and the refusal of enforcement of arbitral awards and their implications for practitioners. The aim of challenging an award before a national court at the seat, or place, of arbitration is to have it modified in some way by the relevant court, or more usually, to have that court declare that the award is to be disregarded (i.e. "annulled" or "set aside") in whole or in part. If an award is set aside or annulled by the relevant court, it will usually be treated as invalid and accordingly unenforceable, not only by the courts of the seat of arbitration but also by national courts elsewhere. This is because, under both the 1958 New York Convention and the UNCITRAL Model Law, the competent court may refuse to grant recognition and enforcement of an award that has been "set aside" by a court of the seat of arbitration. The New York Convention set out various grounds for refusal of recognition and enforcement of an arbitration award. The provisions of the Model Law governing recognition, enforcement or setting-aside of awards are almost identical to those set out in the Convention. Especially, the New York Convention and the Model Law state that an arbitral award may be refused and set aside if a national court of the place of arbitration finds that the award is in conflict with the public policy of its own country. Each state has its own concept of what is required by its "public policy". It is possible to envisage, for example, a dispute over the division of gaming profits from a casino. In many states, the underlying transaction that led to the award would be regarded as a normal commercial transaction and the award would be regarded as valid. Indeed, it is a consistent theme to be found in the legislation and judical decision of many countries. If a workable definition of "international public policy" could be found, it would provide an effective way of preventing an award in an international arbitration from being set aside and refusal for purely domestic policy consideration.

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CONCERNING THE GIJUTSUSHI ACT IN JAPAN, REVISED (일본기술사법의 개정에 대하여)

  • Yasuhito Taki
    • Proceedings of the Korean Professional Engineer Association Conference
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    • 1983.12a
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    • pp.71-73
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    • 1983
  • I. History of the Gijutsushi Act revised II. The Revised Parts of the Act (1) The Revision of the Act; the Gijutsushi-in-Training newiy established 1) The Preliminary Examination was abolished. 2) The Normal Examination consists of two parts, i.e. 1st Stage Examination and 2nd Stage Examination. 3) Those who have passed the 1st Stage Examination are entitled to be Gijutsushi in Training: those who have passed the 2nd Stage Examination are entitled to be Gijutsushi. 4) The Gijutsushi-in-Training will register with the Science and Technology Agency and assist the professional business of Gijutsushi by using the title of Gijutsushi in Training. 5) Requirements for the Examinations. (a) No professional experience will be required for those who wish to apply for the 1st Stage Examination. (b) Those who wish to apply for the 2nd Stage Examination are required to have assisted Gijutsushi as Gijutsushi in Training for the period designated in the ordinance issued from the Prime Ministers' Office (7 years, probably) or mere. (c) All restrictions due to educational careers were abolished as to both kinds of Examinations. 6) Under certain conditions, the 1st Stage Examination will partially be waived. (2) The Subrogation of the Examinations The Director-General of the Science and Technology Agency is able to designate another agency to conduct, on its behalf, the affairs related to the Examinations. (3) The Subrogation of Affairs related to the Registrations The Director-General of the Science and Technology Agency is able to designate another agency to conduct the affairs related to the registration of Gijutsushi and Gijutsushi-in- Training. (4) The Symplification of Registration, etc. 1) The address will be deleted from the matters to be registered. 2) The address and the site of office will be deleted from the matters to be inserted in the Certificate of Registration.

A Study on the Ouabain-induced Transient Inward Current(TI) in the Rabbit Sinoatrial Node (동방결절에서 Ouabain에 의하여 발생하는 일과성 내향전류(TI)에 관한 연구)

  • Choi, Jung-Yun;Hong, Chang-Yee;Earm, Yung-E
    • The Korean Journal of Physiology
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    • v.19 no.2
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    • pp.101-111
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    • 1985
  • Transient inward current (TI) was studied by the two micro-electrode voltage clamp technique in the sinoatrial node of the rabbit. The author confirmed that in $10^{-6}$ M ouabain TI was found in the SA node and investigated the effects of ions, $(Na^+,\;K^+,\;Ca^{2+})$, $\beta-agonist$ (isoprenaline), local anesthetics (quinidine, lidocaine) and Ca-blockers ($Co^{2+}$, verapamil, diltiazem) on the TI recorded during depolarizing voltage clamp pulses to -40 and -20 mV. The results obtained were as follows ; 1) $10^{-6}M$ ouabain increased the frequency of sinus action potential and decreased the amplitude, especially overshoot of action potential. TI was induced by the depolarizing voltage clamp Pulses and the magnitude of the slow inward current (isi) decreased and the time course was slowed by the same depolarizing pulses. 2) 30% $Na^{+}$ and 24mM $K^+$ decreased by $10^{-6}M$ ouabain and 6 mM $Ca^{2+}$ and $10^{-7}M$ isoprenaline increased TI, $i_{si}$ and current oscillations. 3) Quinidine $(5\times10^{-7}M)$ reduced TI and $i_{si}$ but lidocaine $(10^6\;-10^5M)$ didn't reduced or increase TI. Current oscillations increased and isi decreased by lidocaine. 4) Ca-blockers decreased the amplitude and the frequency of sinus action potential. TI and $i_{si}$ decreased significantly but were not abolished completely at the concentrations used in this experiment. Verapamil and diltiazem had inhibitory action on TI in $2\times10^{-7}M$ concentration and showed very slow recovery after wasting out with normal Tyrode solution.

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Development of Potato Virus Y-Resistant Transgenic Potato (감자 바이러스 Y 저항성 형질전환 감자 개발)

  • PARK, Young Doo;RONIS D.H.;DUYSEN M.E.;CHENG Z.M.;LORENZEN J.H.
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.5
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    • pp.313-317
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    • 1997
  • Leaf segments of the potato (Solanum tuberosum L.) genotypes, ND860-2, Norchip, Russet Norkotah, Goldrush, and Norqueen Russet were transformed with the coat protein gene of potato virus Y (PVY). The white-skinned genotypes, ND860-2 and Norchip, were easily transformed and regenerated into shoots, whereas the three russet-skinned genotypes had low frequencies of regeneration. Transformed shoots were generally recovered in four to six weeks. Antibody to PVY coat protein detected a single band of 30 kD in western blots of transgenic plants. Transformed plants had a normal phenotype in the greenhouse and many showed a delayed buildup of PVY following inoculation. Several transgenic lines had negative ELISA readings 85 days after inoculation. Transgenic lines which did not show detectable levels of PVY antigen will be further tested for resistance to PVY.

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Evaluation of Performance and Economics of Organic Rankine Cycle Integrated into Combined Cycle Cogeneration Plant (복합열병합발전소에 적용된 유기랭킨사이클의 성능 및 경제성 평가)

  • Kim, In Seop;Kim, Chang Min;Kim, Tong Seop;Lee, Jong Jun
    • The KSFM Journal of Fluid Machinery
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    • v.20 no.1
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    • pp.41-47
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    • 2017
  • This study aimed to analyze organic Rankine cycle(ORC) which recovers discarded heat from a gas turbine based combined cycle cogeneration(CC-cogen) plant in terms of both performance and economics. The nominal electric power of the CC-cogen plant is around $120MW_e$, and heat for district heating is $153MW_{th}$. The major purpose of this study is to compare various options in selecting heat source of the ORC. Three heat sources were compared. Case 1 uses the exhaust gas from the HRSG, which is purely wasted to environment in normal plant operation without ORC. Case 2 also uses the exhaust gas from the HRSG. On the other hand, in this case, the DH economizer, which is located at the end of the HRSG, does not operate. Case 3 generates power using some of the district heating water which is supplied to consumers. The estimated ORC power generation ranges between 0.3 to 2.3% of the power generation capacity of the CC-cogen plant. Overall, Case 3 is evaluated to be better than other two options in terms of system design flexibility and power generation capacity.