• Journal of Gastric Cancer
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• v.20 no.2
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• pp.212-224
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• 2020

Purpose: miR-205 is a tumor suppressor and plays an important role in tumor invasiveness. However, the role of miR-205 in human gastric cancer (GC) epithelial-mesenchymal transition (EMT) remains unclear. The aim of this study was to investigate the molecular mechanism of miR-205 in the regulation of EMT in GC invasion. Materials and Methods: Quantitative polymerase chain reaction (qPCR) was used to detect the expression of miR-205 in GC. Further, the correlation between the pathological parameters and prognosis of GC was statistically analyzed. A transwell model was used to evaluate the effect of miR-205-3p on the invasion and migration of GC cells. qPCR, western blotting, and luciferase assay were performed to analyze the relationship and target effects between miR-205-3p and the expression of zinc finger electron box binding homologous box 1 (ZEB1) and 2 (ZEB2). Results: We found that the levels of miR-205-3p were significantly lower (P<0.05) in GC tissues than in matched normal tissues. Additionally, the expression of miR-205-3p was related to the tumor invasion depth, lymph node metastasis, lymph node invasion, and tumor, node, metastasis stage. Patients with lower miR-205-3p expression levels in the tumors had a poorer prognosis. The in vitro assays indicated that miR-205-3p could affect the invasion ability and EMT of GC cells by targeting the expression of both ZEB1 and ZEB2. Conclusions: miR-205-3p promotes GC progression and affects the prognosis of patients by targeting both ZEB1 and ZEB2 to directly influence EMT.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.252-252
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• 2016

최근 학계나 산업계에서 indium tin oxide (ITO)의 높은 전기 전도도 및 광투과율을 이용하여 줄 발열을 기초로 하는 투명 면상 발열체에 대한 연구가 활발히 진행 되고 있다. 하지만 단일 ITO 박막으로 제작한 투명 면상 발열체는 온도가 상승함에 따라 균일하게 발열 되지 않으며, 글라스의 곡면 부분에서 유연성이 부족하여 크랙이 발생하는 다양한 문제점들을 가지고 있다. 이를 해결하기 위해 ITO의 결정화 온도 $160^{\circ}C$ 이상의 고온공정 또는 증착 후 열처리가 필요 하는 추가적인 공정이 필요하다. 따라서 본 연구에서는 단일 ITO 박막의 단점을 개선하는 ITO/Ag/ITO 하이브리드 구조의 투명 면상 발열체를 제작하여 전기적, 광학적 특성을 비교하고 발열량, 온도 균일성, 발열 유지 안정도를 조사하였다. 본 연구에서는 $50{\times}50mm$ 크기의 non-alkali glass (Corning E-2000) 기판 상에 마그네트론 스퍼터링 공정으로 상온에서 ITO/Ag/ITO 박막을 연속적으로 증착 하여 다층구조의 하이브리드 형 투명 면상 발열체를 제조하였다. 박막 증착 파워는 DC (Ag) power 100 W, RF (ITO) power 200 W로 하였으며 ITO박막두께는 40 nm로 고정 시키고 Ag박막 두께는 10 ~ 20 nm로 변화를 주었다. 증착원은 3인치 ITO 단일 타깃(SnO2, 10 wt.%)과 Ag 금속 타깃 (순도 99.99%)을 사용하였으며, 고순도 Ar을 이용하여 방전하였으며 총 주입량은 20 sccm, working pressure는 1.0 Pa을 유지하였다. 증착전 타깃 표면의 불순물 제거와 방전의 안정성을 유지하기 위해 10분간 pre-sputtering을 진행하고 증착하였다. 증착한 박막의 전기적, 광학적 특성은 각각 Hall-effect measurements system (ECOPIA, HMS3000), UV-Vis spectrophotometer (UV-1800, SHIMADZU)으로 측정하였으며, 하이브리드 표면의 구조 및 형상은 field emission-scanning electron microscopy (FE-SEM, Hitachi S-4800)으로 관찰하였다. 또한 투명 면상 발열체의 성능은 0.5 ~ 3 V/cm의 다양한 전압을 power supply (Keithly 2400, USA)를 통해서 시편 양 끝단에 인가한 후 시간에 따른 투명면상 발열체의 표면 온도변화를 infrared thermal imager (IR camera, Nikon)를 이용하여 관찰하였다. 하이브리드 구조를 가진 ITO박막의 두께는 40 nm로 고정 시키고 Ag박막의 두께는 10, 15, 20 nm로 변화를 주었다. 이들 박막의 면저항 값은 각각 5.3, 3.2, $2.1{\Omega}/{\Box}$였으며, 투과도는 각각 86.9, 81.7, 66.5 %였다. 이에 비해 두께 95 nm의 단일 ITO박막의 면저항 값은 $59.5{\Omega}/{\Box}$였으며, 투과도는 89.1 %였다. 하이브리드 구조의 전기적특성은 금속층의 두께가 증가할수록 캐리어 농도 값이 증가함에 따라 비저항 값이 감소되어 면저항 값도 감소된 것이며, 금속 삽입층의 전도특성이 비저항에 큰 영향을 주고 있음을 보여준다. 하지만 금속 층의 두께가 증가할수록 Ag층이 연속적인 막을 형성하여 반사율이 증가함에 따라 투과도가 감소하였다. 따라서 하이브리드 구조를 가진 투명 면상 발열체에 금속 삽입층의 두께 조절은 매우 중요한 인자임을 확인 할 수 있었다. 또한 발열성능을 평가 하기 위해 시편 양 끝단에 3 V전압을 인가한 결과, 금속 삽입층의 두께가 10 nm에서 5 nm씩 증가한 하이브리드 구조를 가진 투명면상 발열체의 최고 온도는 각각 98, 150, $167^{\circ}C$ 였으며, 단일 ITO의 최고 온도는 $32^{\circ}C$였다. 이 것은 동일한 두께 (95 nm)의 단일 ITO 박막과 비교하여 면저항이 낮은 하이브리드 박막의 발열량은 약 $120^{\circ}C$로 발열효율이 매우 우수한 것을 확인 할 수 있었다.

• Journal of Korean Society of Steel Construction
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• v.26 no.6
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• pp.511-522
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• 2014

In this study, an innovative steel stair system is presented which enables rapid erection and high quality control in both residential and office building construction. This system features two lightweight steel stringers of box shape, bolted connections easy to absorb construction tolerance, and stair steps movable transversely (or sliding steps) such that the work space needed for concrete stairway wall could be easily provided. In this type of stairway system, other than providing robust connecting details, ensuring vibration performance is especially important since this system may be vibration-sensitive due to lightweight nature and/or probable low damping. To tackle these issues, a series of full-scale mock-up tests were conducted by using box-shape stringer members with or without concrete-fill. The connection system was shown to be sufficiently stiff and strong, or it remained elastic even under the 160% of service load level. Among the seven stringer alternatives, five exhibited satisfactory vibration performance according to the related North American and European acceptance criteria.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1286-1298
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• 2005

The genus Paenibacillus is a new group of bacilli separated from the genus Bacillus, and most of species have been isolated from soil. In the present study, we collected 450 spore-forming bacilli from the roots of winter crops, such as barley, wheat, onion, green onion, and Chinese cabbage, which were cultivated in the southern part of Korea. Among these 450 isolates, 104 Paenibacillus-like isolates were selected, based on their colony shape, odor, color, and endospore morphology, and 41 isolates were then finally identified as Paenibacillus spp. by 16S rDNA sequencing. Among the 41 Paenibacillus isolates, 23 were classified as P. polymyxa, a type species of the genus Paenibacillus, based on comparison of the 16S rDNA sequences with those of 32 type strains of the genus Paenibacillus from the GenBank database. Thirty-five isolates among the 41 Paenibacillus isolates exhibited antagonistic activity towards plant fungal and bacterial pathogens, whereas 24 isolates had a significant growth-enhancing effect on cucumber seedlings, when applied to the seeds. An assessment of the root-colonization capacity under gnotobiotic conditions revealed that all 41 isolates were able to colonize cucumber roots without any significant difference. Twenty-one of the Paenibacillus isolates were shown to contain the nifH gene, which is an indicator of $N_{2}$ fixation. However, the other 20 isolates, including the reference strain E681, did not incorporate the nifH gene. To investigate the diversity of the isolates, a BOX-PCR was performed, and the resulting electrophoresis patterns allowed the 41 Paenibacillus isolates to be divided into three groups (Groups A, B, and C). One group included Paenibacillus strains isolated mainly from barley or wheat, whereas the other two groups contained strains isolated from diverse plant samples. Accordingly, the present results showed that the Paenibacillus isolates collected from the rhizosphere of winter crops were diverse in their biological and genetic characteristics, and they are good candidates for further application studies.

• Food Science and Preservation
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• v.23 no.2
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• pp.166-173
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• 2016

This study aimed to evaluate the commercialized packaging status of tomato and paprika, and to investigate the effect of different packaging materials on the quality of tomato and paprika during storage. Packaging statuses were surveyed at a department store, wholesale market, and supermarket in Seoul, Korea. Materials used for packaging tomato and paprika were cartons, polypropylene (PP), low-density polyethylene (LDPE), polystyrene (PS), and polyvinyl chloride (PVC). Tomato and paprika were packaged by using corrugated boxes, Styrofoam trays, PP film, and PVC film. The weight loss and hardness of non-packaged tomato and paprika were significantly different after 48 hr to the initial values (p<0.05). Box-packaged tomatoes had the lowest pH values and showed significantly higher soluble solid contents (p<0.05). However, there were no significant differences in among other packaging materials. For paprika, the ${\Delta}E$ values of PVC wrapping were higher than those of other packagings. Hence, the results demonstrated that a corrugated box with PP film and PP film bags with four holes plus wire-tying were most able to maintain the overall qualities of tomato and paprika, respectively, during storage.

• Journal of the Korean GEO-environmental Society
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• v.21 no.4
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• pp.19-30
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• 2020

This numerical study is to investigate the seepage characteristics of the side of the structure in the event of leakage from the structural connection part of the drainage structure installed through the enlarged levee, and to analyze the effect of piping on the stabilization of the levee by the lateral penetration behavior. To take into account lateral seepage behavior, 2D and 3D numerical analyses were performed on the same model, and the effect of lateral seepage was analyzed to assess the validity of the numerical analysis. As a result, when leakage occurs and a lateral seepage is considered with the gate located on the riverside land, the maximum pore water pressure near the leakage point of the structure has been reduced by half compared to the normal seepage state where no leakage occurred. Excessive variation in the pore pressure was shown at the lower part of the structure, especially if lateral seepage is not considered. As a water level rises to the high water level, it shows the hydraulic gradient was larger than the critical hydraulic gradient, which will be vulnerable to long-term piping. If a gate is located in the inland and side seepage is not considered, the effect of the seepage water such as hydraulic gradient and seepage velocity is underestimated compared with the case of considering side seepage. The maximum hydraulic gradient is relatively small when lateral seepage is neglected if a gate is located in the riverside land and there was might be a risk of piping or loss of material. In addition, the period exceeding the critical hydraulic gradient was interpreted as a short time zone. As a result, it is considered that the possibility of piping can be underestimated if side seepage is ignored.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.3 no.3
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• pp.27-37
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• 1983

A method of dynamic analysis is developed for torsional free vibrations of elliptical-box girder type or stiffening truss system suspension bridge. In this study, the method based on a finite element technique using a digital computer, is illustrated by two numerical examples, the Namhae Bridge which is located in Kyungsang nam-do opened on June, 1973, and the Mt. Chunma Bridge is simple span pedestrian's suspension bridge with lateral bracing system in Mt. Chunma, Kyungki-do, are used. In general, dynamic modes of complex suspension bridges are three-dimensional in form, i.e., coupling between vertical and torsional motions. However, introduced that amplitudes of oscillation are infinitesimal for coincidence with the purpose of it's use, thereupon, the torsional vibration analyses are treated without coupling terms. A sufficient number of natural frequencies and mode shapes for torsional free vibration are presented in this paper. In the case of Mt. Chunma Bridge, the natural frequencies and periods are computed with and without reinforcement, respectively, and compared their discrepancies. The influence of the auxiliary reinforcing cables is prevailing in the first few modes, namely, 1st and 2nd in symmetric and 1st, 2nd and 3rd in antisymmetric vibration, and conspicuous in the symmetric compared with the antisymmetric motion, but in the higher modes, this kind of simple accessory elucidates rether converse effects. In the Namhae Bridge, the results are compared with the Manual's obtained by wind tunnel test. It reveals commendable agreement.

• Journal of the Korean Vacuum Society
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• v.16 no.5
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• pp.388-396
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• 2007

KSTAR (Korea Superconducting Tokamak Advanced Research) current lead system (CLS) has a role to interconnect magnet power supply (MPS) in room temperature (300 K) and superconducting (SC) bus-line, electrically. For the first plasma experiments, it should be assembled 4 current leads (CL) on toroidal field (TF) current lead box (CLB) and 14 leads on poloidal field (PF) CLB. Two current leads, with the design currents 17.5 kA, and SC bus-lines are connected in parallel to supply 35 kA DC currents on TF magnet. Whereas, it could supply $20\;{\sim}\;26\;kA$ to each pairs of PF magnets during more than 350 s. At the cold terminals of the leads, there are joined SC bus-lines and it was constructed helium coolant control system, aside from main tokamak system, to protect heat flux through current leads and enhanced Joule heat due to supplied currents. Throughout the establishment processes, it was tested the high vacuum pumping, helium leak of the helium lines and hardwares mounted between the helium lines, flow controls for CL, and liquid nitrogen cool-down of possible parts (current leads, CL helium lines, and thermal shield helium lines for CLB), for the accomplishment of the required performances.

• Restorative Dentistry and Endodontics
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• v.18 no.2
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• pp.291-316
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• 1993

Restorative procedures can lead to tooth fracture due to the relatively small amount of the remaining tooth structure. It is essential to prevent fractures by having a clear concept of the designs for cavity preparations. Among the several parameters in cavity designs, profound understanding of isthmus width factor would facilitate selection of the appropriate cavity preparation for a specific clinical situation. In this study, MO amalgam cavity were prepared on maxillary first premolar and filled with amalgam. Three dimensional, model with 1365 8-node brick elements was made by serial photographic method. In this model, isthmus was varied in width at 1/4, 1/3, 1/2 and 2/3 of intercuspal width and material properties were given for three element groups, i.e., enamel, dentin and amalgam. A load of 500 N was applied vertically on amalgam and enamel. In case of enamel loading, 2 model (with and without amalgam) was compared to consider the possibility of play at the interface between tooth material and amalgam. These models were analyzed with three dimensional finite element method. The results were as follows: 1. The stress was concentrated on the facio-pulpal line angle and distal marginal ridge of the cavity. 2. With the increase of the isthmus width, the stress spread around the facio-pulpal line angle and the area of stress concentration moved toward the proximal box. 3. In case of narrow isthmus width, the initiation point of crack would be in the area of isthmus corner of the cavity, and with the increase of the isthmus width, it would move toward the proximal box and at the same time the possibility of crack increase at the distal marginal ridge. 4. The direction of crack progressed outward and downward from the facio-pulpal line angle, and with the increase of the isthmus width, it approximated vertical direction. At the marginal ridge, it occurred in vertical direction. 5. It would be favorable to make the isthmus width narrower than a third of the intercuspal width, and to cover the cusp if isthmus width were wider than half of the intercuspal width. 6. It is necessary to apply the possibility of play to the finite element analysis.

• Economic and Environmental Geology
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• v.44 no.6
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• pp.475-483
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• 2011

Quantitative estimate of manganese nodules based on the operation of a free fall grab (FFG) needs to be corrected because of its less retrieval ability. Previously, the correction parameter of the nodule abundance collected by FFG was calculated based on the image analysis of the photos of bottom sediment in the northern sector of the nodule exploration area of Korea in the NE equatorial Pacific. However, nodules in the southern sector are commonly covered by sediment, which may prevent the use of the correction parameter estimated by photographic techniques. In this study, we attempted dual nodule sampling at the same location by different equipments (i.e. box corer (BC) and FFG) to examine the previous correction parameter of nodule abundance for FFG operation. During the exploration cruises in 2007 to 2009, Mn-nodules were collected from 40 stations both by BC and FFG in the southern sector. The correlation analysis between BC and FFG operations revealed that the BC collected nodules 1.43 times larger than FFG. Our result suggests that the correction parameter of 1.43 can be applied for collection of FFG data to estimate Mn-nodule distribution in the southern sector. The obtained parameter is similar to the previous parameter (1.42~1.45) calculated by the image analysis method, indicating an usefulness of new correction parameter suggested by this study.