• International Journal of Knowledge Content Development & Technology
• /
• 제11권3호
• /
• pp.81-112
• /
• 2021

In this study, users' perceptions and concerns about digital information resources were investigated, focusing on impediments to the use of digital resources, to devise measures for improvement by identifying factors that hinder the use of digital information resources. To achieve the purpose of the study, the research identifies the inconvenien ce factors and establishes the user inconvenience list based on prior research and the current status survey. Based on the derived list, a survey was conducted on users of public libraries. Through this study, it was found that the use of digital information resources can be enhanced by improving the internal library system. Specifically, it refers to improving search performance and accessibility of digital data, providing one-stop services for digital information resources through the operation of an integrated data management system, conducting customized user education, and establishing a digital information resource preservation system, e.g. web archiving. Also, improving the external environment and system, such as contracting with more libraries for digitized data use agreements, strengthening support, unifying the names and classifications of digital information resources, and simplifying the procedure for using digital information resources, is of great importance.

• Journal of Ginseng Research
• /
• 제43권1호
• /
• pp.116-124
• /
• 2019

Background: Ginsenosides are known as the principal pharmacological active constituents in Panax medicinal plants such as Asian ginseng, American ginseng, and Notoginseng. Some ginsenosides, especially the 20(R) isomers, are found in trace amounts in natural sources and are difficult to chemically synthesize. The present study provides an approach to produce such trace ginsenosides applying biotransformation through Escherichia coli modified with relevant genes. Methods: Seven uridine diphosphate glycosyltransferase (UGT) genes originating from Panax notoginseng, Medicago sativa, and Bacillus subtilis were synthesized or cloned and constructed into pETM6, an ePathBrick vector, which were then introduced into E. coli BL21star (DE3) separately. 20(R)-Protopanaxadiol (PPD), 20(R)-protopanaxatriol (PPT), and 20(R)-type ginsenosides were used as substrates for biotransformation with recombinant E. coli modified with those UGT genes. Results: E. coli engineered with $GT95^{syn}$ selectively transfers a glucose moiety to the C20 hydroxyl of 20(R)-PPD and 20(R)-PPT to produce 20(R)-CK and 20(R)-F1, respectively. GTK1- and GTC1-modified E. coli glycosylated the C3-OH of 20(R)-PPD to form 20(R)-Rh2. Moreover, E. coli containing $p2GT95^{syn}K1$, a recreated two-step glycosylation pathway via the ePathBrich, implemented the successive glycosylation at C20-OH and C3-OH of 20(R)-PPD and yielded 20(R)-F2 in the biotransformation broth. Conclusion: This study demonstrates that rare 20(R)-ginsenosides can be produced through E. coli engineered with UTG genes.

• 수산해양기술연구
• /
• 제58권1호
• /
• pp.39-48
• /
• 2022

Using environmental DNA (eDNA) in the fisheries and oceanography fields, research on the diversity of biological species, the presence or absence of specific species and quantitative evaluation of species has considerably been performed. Up to date, no study on eDNA has been tried in the area of fisheries acoustics in Korea. In this study, the biomass of a dominant species in the northwestern waters of Jeju Island was examined using 1) the catch ratio of the species from trawl survey results and 2) the ranking ratio of the species from the eDNA results. The dominant species was Zoarces gillii, and its trawl catch ratio was 68.2% and its eDNA ratio was 81.3%. The Zoarces gillii biomass from the two methods was 7199.4 tons (trawl) and 8584.6 tons (eDNA), respectively. The mean and standard deviation of the acoustic backscattering strength values (120 kHz) from the entire survey area were 135.5 and 157.7 m²/nm², respectively. The strongest echo signal occurred at latitude 34° and longitude 126°15' (northwest of Jeju Island). High echo signals were observed in a specific oceanographic feature (salinity range of 32-33 psu and the water temperature range of 19-20℃). This study was a pilot study on evaluating quantitatively aquatic resources by applying the eDNA technique into acoustic-trawl survey method. Points to be considered for high-quality quantitative estimation using the eDNA to fisheries acosutics were discussed.

• 한국축산식품학회지
• /
• 제42권4호
• /
• pp.593-608
• /
• 2022

The effect of hot-air dried Lentinula edodes pileus (DLE) on the quality and organoleptic properties of rolled-dumplings was evaluated. DLE was prepared by drying at 60℃ for 24 h and added (Non, 7%, and 9%) to rolled-dumplings. The proximate composition, pH, color (CIE L^*, a^*, b^*), and cooking yield were analyzed. Texture profile analysis, electronic-nose (e-nose), electronic-tongue (e-tongue), and organoleptic evaluation were also conducted. The cooking yield of dumplings with 9% DLE was significantly lower than that of the congeners without DLE, whereas 7% DLE did not lead to significant differences compared without DLE. With increasing DLE addition, the pH and lightness of the dumplings decreased significantly, whereas the redness tended to increase. The texture profile was significantly higher for the dumplings with DLE compared to those without DLE. E-nose analysis confirmed that DLE addition led to the positive odors (methanethiol: meaty, sulfurous; 3-methylbutanal: malty, toasted) and the negative odors (trimethylamine: ammoniacal; acetic acid: acidic, sour). E-tongue analysis showed that DLE addition decreased the intensity of the sourness and increased the intensity of the saltiness and umami of rolled-dumplings. DLE addition improved the overall organoleptic properties, but 9% DLE can be recognized as a foreign substance in organoleptic acceptance. Consequently, DLE has the potential to serve as a flavor and odor enhancer for rolled-dumplings, and the addition of DLE can positively improve consumer acceptance by improving the quality and organoleptic properties.

• 한국자원식물학회지
• /
• 제27권6호
• /
• pp.671-679
• /
• 2014

Molecular phylogenetic analyses of Korean Elaeagnus L. were conducted using seven species, one variety, one forma and four outgroups to evaluate their relationships and phylogeny. The sequences of internal transcribed spacer regions in nuclear ribosomal DNA were employed to construct phylogenetic relationships using maximum parsimony (MP) and Bayesian analysis. Molecular phylogenetic analysis revealed that Korean Elaeagnus was a polyphyly. E. umbellata var. coreana formed a subclade with E. umbellata. Additionally, the genetic difference between E. submacrophylla and E. macrophylla was very low. Moreover, E. submacrophylla formed a branch from E. macrophylla, indicating that E. submacrophylla can be regarded as a variety. However, several populations of this species were not clustered as a single clade; therefore, further study should be conducted using other molecular markers. Although E. glabra f. oxyphylla was distinct in morphological characters of leaf shape with E. glabra. But E. glabra f. oxyphylla was formed one clade by molecular phylogenetic with E. glabra. Additionally, this study clearly demonstrated that E. pungens occurs in Korea, although it was previously reported near South Korea in Japan and China. According to the results of ITS regions analyses, it showed a resolution and to verify the relationship between interspecies of Korean Elaeagnus.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2000년도 춘계총회 및 학술대회
• /
• pp.211.1-211.1
• /
• 2000

• 한국자원식물학회지
• /
• 제37권2호
• /
• pp.120-129
• /
• 2024

활성산소종은 미토콘드리아 대사활동을 통해 생성되는 반응성이 강한 산소 함유 분자로서 특히 간에서 복잡한 세포 분자와 반응하여 산화적 스트레스를 유발하는 물질이다(Choi et al., 2012). 에탄올 대사 과정에서는 ROS가 생성되어 산화스트레스를 유발하며, 과도한 에탄올은 ROS를 제거하는 항산화 시스템을 억제함으로써 지방, 단백질 또는 DNA와 같은 생체 분자에 산화 손상을 유발하여 세포를 손상시킨다(Albano, 2006; Koch et al., 2004). 본 연구에서는 오리나무 잎 추출물(AJL)에서 알코올(EtOH)로 유발된 산화적 스트레스에 대한 간세포 손상의 보호 효과를 평가하기 위해 HepG2/2E1 세포에서 EtOH와 AJL 및 대조군silymarin을 각각 처리한 후 세포생존율을 평가하고 세포독성이 없는 범위를 설정하여 활성산소종 생성량, 항산화 관련 지표(DPPH, ABTS, MDA, GSH) 및 간 손상 지표(AST 및 ALT)를 측정하였다. 알코올에 대한 간 보호 효과에서는 알코올만 처리한 군의 세포생존율이 53.8%로 감소하였으나 AJL을 함께 처리 군에서 세포생존율이 85.1%의 유의적인 증가로 간 보호효과를 확인하였다. 다음으로 AJL에서 세포 내 ROS 생성 억제능은 에탄올 처리군에서 정상군에 비해 유의적으로 ROS 생성이 증가하였으나 AJL 농도별 처리시 정상군 수준으로 ROS 생성을 현저하게 감소시켰다. MDA 함량은 에탄올 처리군에서 무처리군에 비해 유의적으로 증가하였으나 AJL 처리시 농도 의존적으로 에탄올 처리군에 비해 유의적인 차이를 보이며 MDA 함량을 감소시켰다. GSH 함량은 에탄올 처리군에서 정상군보다 유의적으로 함량이 감소하였으나 AJL 농도별 처리군 모두 에탄올 처리군에 비해 GSH 함량을 증가시켰다. 또한 간손상 지표로 활용되는 AST 및 ALT 활성 변화 확인 시, 에탄올 처리에 따른 간세포 손상군에 비하여 AJL 처리군에서는 농도 의존적으로 현저하게 감소시켰으며, 대조군silymarin과 비교하여 유사한 감소된 결과를 관찰하였다. 이상의 결과로부터 종합해 볼때AJL은 간손상에 대한 보호 및 간손상 억제 가능성을 기대할 수 있으며, 간기능 개선 효과에 대한 건강기능식품개발의 기초자료로서 활용될 수 있을 것이라 사료된다.

• 한국축산식품학회지
• /
• 제40권4호
• /
• pp.668-674
• /
• 2020

Manuka honey (MH) has been shown anti-bacterial activity against several pathogenic bacteria. However, the inhibitory effect of MH on biofilm formation by Escherichia coli O157:H7 has not yet been examined. In this study, MH significantly reduced E. coli O157:H7 biofilm. Moreover, pre- and post-treatment with MH also significantly reduced E. coli O157:H7 biofilm. Cellular metabolic activities exhibited that the viability of E. coli O157:H7 biofilm cells was reduced in the presence of MH. Further, colony forming unit of MH-treated E. coli O157:H7 biofilm was significantly reduced by over 70%. Collectively, this study suggests the potential of anti-biofilm properties of MH which could be applied to control E. coli O157:H7.

• 한국자원식물학회지
• /
• 제8권3호
• /
• pp.253-258
• /
• 1995

1988년 7월부터 1991년 9월까지 전주와 근교의 만덕산, 위봉사 그리고 내장산 국립공원, 발왕산, 그리고 월출산 국립공원에서 많은 고등균류를 채집하여 동정하였다. 그 결과 한국산 미기록종으로 확인 된 것은 다음과 같다. 빨강꽃버섯(Hygrocybe marchii), 헛물털애주름버섯(Hydropus floccipes), 뿔껍질갓버섯(Lepiota hystrix), 사초외대버섯(Entoloma cinchonense), 물외대버섯(E.hypoporhryum), 그리고 봉밀외대버섯(E,mellum)이다.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2012년도 학술발표회
• /
• pp.780-780
• /
• 2012

하천에서의 1차원 수질해석에 널리 사용되는 QUAL2E 모형은 전체 대상구간을 요소(element)로 구성하고 다시 수리학적으로 유사한 흐름특성을 가진 구간(reach)으로 구분하고 있다. 각 구간에서는 동일한 유속과 수심이 형성되는 것으로 가정하고 있기 때문에 자연하도의 다양한 흐름변화, 특히 자연하천의 보나 낙차공으로 인한 국부적인 흐름상태를 모형에 반영하기 어려우며, 이로 인한 실제 흐름특성과의 차이는 수질모의 해석결과에도 다양한 영향을 미치게 된다. QUAL2E 모형에서는 이와 같은 보나 낙차공의 수리학적 특성을 반영하기 위해 댐 계수옵션이 있으며, 이를 통해 흐름으로 인한 폭기 현상을 재현하도록 되어 있다. 본 연구에서는 모형 내 재폭기 계수에 대한 민감도 검토를 수행하였으며, 한강의 팔당댐부터 한강대교까지를 대상구간으로 잠실수중보로 인해 발생하는 재폭기 현상에 관해 연구하였다. 본 연구는 보나 낙차공이 있는 하천에 대해 QUAL2E 모형을 적용할 경우 용존산소(DO)를 비롯한 여러 가지 수질변수의 정확도 높은 예측에 기여할 것으로 기대된다.