• Asian-Australasian Journal of Animal Sciences
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• 제29권5호
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• pp.666-673
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• 2016

Two experiments were conducted to evaluate the effects of the level of corn dry distillers grains with solubles (CDDGS) supplementation on growing performance, blood metabolites, digestion characteristics and ruminal fermentation patterns in steers grazing dormant forage. In Exp. 1, of growth performance, 120 steers ($204{\pm}5kg$ initial body weight [BW]) were distributed randomly into 3 groups (each of 40 steers), which were provided with the following levels of CDDGS supplement: 0%, 0.25%, or 0.50% BW. All groups of steers were grazed for 30 days in each of 3 grazing periods (March, April, and May). Approximately 1,000 ha of the land was divided with electric fencing into 3 equally sized pastures (333 ha in size). Blood samples were collected monthly from 20 steers in each grazing group for analysis of glucose (G), urea-nitrogen (UN) and non-esterified fatty acids. Final BW, average daily gain (ADG) and supplement conversion (CDDGS-C) increased with increasing levels of CDDGS supplementation (p<0.05).The CDDGS supplementation also increased the plasma G and UN concentrations (p<0.05). In Exp. 2, of digestive metabolism, 9 ruminally cannulated steers ($BW=350{\pm}3kg$) were distributed, following a completely randomized design, into groups of three in each pasture. The ruminally cannulated steers were provided the same levels of CDDGS supplementation as in the growing performance study (0%, 0.25%, and 0.50% BW), and they grazed along with the other 40 steers throughout the grazing periods. The dry matter intake, crude protein intake, neutral detergent fiber intake (NDFI), apparent digestibility of dry matter (ADDM), crude protein (ADCP) and neutral detergent fiber (ADNDF) increased with increasing levels of CDDGS supplementation (p<0.05). The ruminal degradation rates of CP (kdCP), NDF (kdNDF) and passage rate (kp) also increased with increasing levels of CDDGS supplementation (p<0.05). Ruminal ammonia nitrogen ($NH_3$-N) and propionate concentrations also increased with increasing levels of CDDGS supplementation (p<0.05). However, acetate concentrations decreased with increasing levels of CDDGS supplementation (p<0.05). Liquid dilution rate increased with increasing levels of CDDGS supplementation but ruminal liquid volume decreased (p<0.05). On the basis of these findings, we can conclude that CDDGS supplementation enhanced the productive performance of cattle grazing native rangeland without negatively affecting forage intake, glucose and urea-nitrogen blood concentrations, ruminal degradation and ruminal fermentation patterns.

• 인터넷정보학회논문지
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• 제13권2호
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• pp.41-49
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• 2012

스마트 폰 이용자의 급격한 증가에 따른 무선 네트워크의 지원 및 모바일 환경은 언제 어디서나 네트워크를 이용할 수 있게 되었다. 이러한 인터넷 망의 발달로 인해 네트워크 트래픽이 급증함으로써 네트워크를 통한 분산서비스 공격, 인터넷 웜, 이메일 바이러스 등의 다양한 악의적인 공격이 증가되고 이에 따른 패턴이 급격하게 증가하는 추세이다. 기존 연구에서 침입탐지시스템인 Snort 2.1.0 룰의 약 2,000개 패턴으로 M-바이트 점핑 윈도우 알고리즘을 적용한 결과를 분석하였다. 하지만 점핑 윈도우 알고리즘은 패턴의 길이와 수에 큰 영향을 받기 때문에 더 긴 패턴과 더 많은 패턴을 갖는 새로운 환경(Snort 2.9.0)에서 TCAM 룩업 횟수와 TCAM 메모리 크기에 대한 새로운 분석이 필요하다. 이 논문에서는 Snort-2.9.0 룰에서 약 8,100개의 패턴을 이용하여 윈도우 크기별 TCAM 룩업 횟수와 TCAM의 크기를 시뮬레이션 했고 그 결과를 분석하였다. Snort 2.1.0에서는 16-바이트 윈도우에서 9Mb의 TCAM이 최적을 효과를 낼 수 있는 반면, Snort 2.9.0에서는 16-바이트 윈도우에서 18Mb TCAM 4개를 캐스케이딩으로 연결할 경우 최적의 효과를 낼 수 있다.

• 한국대기환경학회지
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• 제28권5호
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• pp.595-605
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• 2012

Fine particles ($PM_{2.5}$) were collected and analyzed from April 2010 through January 2011 in Chungju to investigate the characteristics of $PM_{2.5}$ and its ionic species. The annual mean concentrations of $PM_{2.5}$, ${SO_4}^{2-}$, $NO_3{^-}$, and $NH_4{^+}$ in the particulate phase were 40.84, 7.61, 7.14 and $3.74{\mu}g/m^3$, respectively. $PM_{2.5}$ concentrations were higher in fall and spring than in winter and summer. The elevated concentrations episodes are the main factor that enhanced the $PM_{2.5}$ concentrations in the fall. Among the major ionic species ${SO_4}^{2-}$ showed the highest concentration, followed by $NO_3{^-}$ and $NH_4{^+}$, $NO_3^-$ exhibited higher concentrations during the winter, but ${SO_4}^{2-}$ and $NH_4{^+}$ were not showed seasonal variation. The high correlations were found among $PM_{2.5}$, ${SO_4}^{2-}$, $NO_3{^-}$ and $NH_4{^+}$ during all seasons except for spring. The evaluation of backward trajectories and meteorological records show that the highest $PM_{2.5}$ concentration levels occurred during W-NW weather conditions, which influenced by the emission sources of China area. The low pollution levels generally occurred during E-S weather conditions, which influenced by the East Sea and south of the Yellow Sea. The elevated $PM_{2.5}$ mass concentrations arouse the concentration of $NO_3{^-}$, but no effects on ${SO_4}^{2-}$ and $NH_4{^+}$.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.559-565
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• 1998

This study was conducted to investigate the effects of different feeding regimen on the growth and nutrients excretion in finishing pigs. A total of 80 pigs (59.91 kg BW) and 6 pigs (85 kg BW) were employed in a growth trial and metabolic trial, respectively. Treatments were grower-grower (GG), grower-early finisher(GE), early finisher-early finisher (EE), and early finisher-late finisher (EL) diet feeding regimens for feeding trial and G, E, L diets for metabolic trial. Through whole experimental period, no significant difference was found in any of the criteria measured. Pigs fed on a GG regimen gained slightly faster than pigs fed on other feeding regimens, with no significant difference. Although there was a trend that pigs fed on a GG or GE regimen showed improved feed/gain over pigs fed on a EE or EL regimen, the difference also was not significant. In the metabolic trial, dry matter and phosphorus digestibility was not affected by diets, however, phosphorus digestibility tended to be decreased as nutrients contents was decreasing in the diets. Crude protein digestibility was significantly influenced by the nutrients contents in th diets. The data suggested that 11.7% crude protein seemed to be low for the 85 kg pigs. Though nutrients excretion was not significantly influenced by dietary treatment except phosphorus, there was a trend that pigs fed diets with low nutrient content excreted less amount of nitrogen and phosphorus than pigs fed diets with high nutrients content. There was a trend that GG or GE feeding regimen gad a favorable effect on carcass grade when only 10 lighter pigs of each treatment were subjected to statistical analysis. However, with 10 heavier pigs, EE and EL feeding regimen showed better carcass grade, though the difference was not significant. In summary, it seemed that producers generally oversupply the expensive nutrients for the finishing pigs. High nutrient diets do not always guarantee high growth rate of pigs. It rather seems that to meet nutrient requirements for the each growth phase is more important in economical pork production.

• Journal of Gastric Cancer
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• 제20권2호
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• pp.139-151
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• 2020

Purpose: Globally, there is a high incidence of gastric cancer (GC). Leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) is reported to play a vital role in several human malignancies. However, there is limited understanding of the role of LETM1 in GC. This study aims to investigate the effects of LETM1 on proliferation, migration, and invasion of GC cells. Materials and Methods: The expression levels of LETM1 in the normal gastric mucosal epithelial cells (GES-1) and GC cells were analyzed by quantitative real-time polymerase chain reaction and western blotting. CCK-8, wound healing, and Transwell invasion assays were performed to evaluate the effect of LETM1 knockdown or overexpression on the proliferation, migration, and invasion of the GC cells, respectively. Additionally, the effect of LETM1 knockdown or overexpression on GC cell apoptosis was determined by flow cytometry. Furthermore, the effect of LETM1 knockdown or overexpression on the expression levels of PI3K/Akt signaling pathway-related proteins was evaluated by western blotting. Results: The GC cells exhibited markedly higher mRNA and protein expression levels of LETM1 than the GES-1 cells. Additionally, the knockdown of LETM1 remarkably suppressed the GC cell proliferation, migration, and invasion, and promoted the apoptosis of GC cells, which were reversed upon LETM1 overexpression. Furthermore, the western blotting analysis indicated that LETM1 facilitates GC progression via the PI3K/Akt signaling pathway. Conclusions: LETM1 acts as an oncogenic gene to promote GC cell proliferation, migration, and invasion via the PI3K/Akt signaling pathway. Therefore, LETM1 may be a potential target for GC diagnosis and treatment.

• IMMUNE NETWORK
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• 제1권1호
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• pp.36-44
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• 2001

Background : Peritoneal metastasis is one of the maj or types of the stomach cancer recurrence and the role of the adhesion molecules is thought to be very much important in this event. Retinoic acid (RA) has been known to induce the growth inhibition and differentiation of various malignancies, and apoptpsis and the change of expression of adhesion molecules have been reported to be involved in the action of RA. Methods : We studied the adhesion abilities of SNU-1, SNU-5, and SNU-6 cells to the peritoneal endothelial cells as well as the expression of the adhesion molecules (CD44, ICAM-1) in Western blot analysis. And also we studied the expression of apoptosis and the change of expression patterns of the various isoforms of CD44 and the change of the adhsion abilities of the cell line cells after RA treatment. Results: CD44 was expressed in SNU-5 and -16, together with an isoform in SNU-16. ICAM-1 was not expressed in any of the cell line cells tested. After the treatment of RA in the concentration range of $1-5{\times}10^{-5}M$ to three stomach cancer cell lines, growth inhibition, apoptosis and the change of expression of the CD44 were noted. After RA treatment, the expression of CD44H was weakly increased in SNU-1, and was markedly increased in SNU-5. In SNU-16, the expression of CD44H was decreased while that of CD44E were markedly increased. The adhesibility of cells to peritoneal cells was increased in relation with the increase of the CD44H expression, which shows the fact that the adhesibility of tumor cells to peritoneal mesothelial cells is mediated by CD44H recognizing hyaluronic acid. Conclusion : RA induces growth inhibition of stomach cancer cell line cells and increase the adhesiblity of stomach cancer cell line cells to peritoneal mesothelium. It is believed that RA decreases the metastatic ability of stomach cancer cells by upregulating the CD44H expression.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2017년도 춘계공동학술대회
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• pp.58-58
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• 2017

점적 호스의 원통형 미로 구조물내의 유동특성을 분석하기 위해 금속판에 동일한 구조의 미로가 가공된 시험부를 활용하여 압력과 유량의 변화에 대한 실험을 CFD 해석하였다. 해석은 미로의 길이에 따른 유량 특성을 정밀하게 분석하기 위해 전체 미로에 구간별 총 8개의 배출구를 배치하고 밸브 조절을 통해 구간별 압력에 따른 유량을 측정함으로서 실험에 의한 압력과 유량의 관계를 검증하고 새로운 경질 미로를 설계하는데 응용하고자 한다. 경질 미로의 이론적인 해석에서 정밀도가 실험을 대신할 가능성이 있는지에 관심을 두었다. 점적관수의 경질 미로는 디자인을 달리함으로써 성능이 높고 미세한 스케일이 침착되지 않는 고성능의 점적관수 장치의 개발이 가능할 것으로 판단하였다. 미로 입구의 공급 유체 압력(Gauge pressure)을 0.5, 1.5, 2.5, 3.0 bar로 나누어 공급하였으며, 8개의 구간별 유출되는 유량은 전자저울에서 측정하였다. 향후 최적 미로의 새로운 설계를 위해 미로구조 전체를 CFD 해석하였고 그 결과를 실험치와 비교 분석하였다. 또한 경질 미로내 각 구간별 세부적인 유동구조를 분석하였고 해석결과와 실험결과를 통해 향후 새로운 경질 구조의 개발이 가능 할 것으로 판단되었다. 경질 미로의 입구압력에 따른 미로의 길이 #1과 #2에 대한 CFD 분석에서는 전체적으로 경질 미로에 압력이 증가할수록 미로의 유체 속도가 증가됨을 알 수 있었으며 이것은 #2에서도 거의 같은 양상을 나타내었다. 미로 통로에서는 와류로 인한 이물질의 멈춤 현상이 발생하지 않을 만큼 흐름이 원활함을 알 수 있었다. 미로의 길이 #3과 #4에 대한 CFD 분석과 미로의 길이 #5와 #6에 대한 CFD 분석에서는 압력이 증가하고 미로의 길이가 길어질수록 속도에도 영향을 미치고 있는 것으로 나타났으며 미로와 미로 사이의 넒은 통로에서는 유속이 크게 떨어지고 있음을 알 수 있었다. 따라서 이 부분에는 유체속의 이물질이 침착할 수 있는 가능성이 있으며 통로가 좁은 곳에서는 유속이 증가되는 것으로 나타났다. #7과 #8은 미로의 상하단으로 유출되는 부분의 차이이므로 실질적으로 해석의 결과는 차이가 없었다. 경질 미로에 대한 압력에 따른 미로의 길이별 유출량은 지수적인 반비례 현상을 나타내어 앞서 실험한 연구의 결과와 1~2% 오차 범위에서 잘 일치하였다. 따라서 점적 호스의 경질 미로에 대한 설계는 이론적인 해석을 통하여 새로운 디자인이 가능할 것으로 판단되었다. 경질 미로의 미로의 길이에 따른 CFD 분석의 결과는 실험적인 유출량의 값과 거의 일치하였다. 미로와 미로 사이의 넒은 통로에서는 유속이 크게 떨어져 이물질들이 침착될 가능성이 있었으며 실질적으로 넓게 설계하는 것이 유리하지 않을 것으로 판단되었다. 경질 미로에 대한 압력에 따른 미로의 길이별 유출량은 지수적으로 반비례 하였으며 점적 호스의 경질 미로에 대한 새로운 설계는 이론적인 해석을 통하여 가능할 것으로 판단되었다.

• Journal of Preventive Medicine and Public Health
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• 제32권1호
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• pp.60-64
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• 1999

환경오염과 관련된 건강효과 연구에서 흡연, 직업 등 교란변수의 영향을 효과적으로 배제할 수 있으면서 폐기능검사가 제대로 시행될 수 있는 연구대상으로 선호되고 있는 특정 연령층인 12세 학동의 보다 정확한 FVC와 FEV1 예측식을 만 들고자 전국 11개 중학교의 학생들(남자 256명, 여자 301명)을 대상으로 측정된 신장, 체중, 그리고 폐기능검사 값으로 신장-체중의 회귀식을 유도하였고, 이를 통해 12세 인구의 신장별 표준체중을 산출하였다. 이 표준체중과 실측체중의 차이인 잔차를 독립변수로 하여 폐기능 예측 식을 남녀별로 만들었는데, 남자의 경우는, FVC(ml) = 50.84 $\times$ 신장(cm) + 7.06 $\times$ 체중 잔차 - 4838.86, FEV1(m1) = 43.57 $\times$ 신장(cm) + 3.16 $\times$ 체증 잔차 4156.66 이었다. 여자에서는 FVC(ml) = 42.57 $\times$ 신장(cm) + 12.50 $\times$ 체중 잔차 - 3862.39, 그리고 FEV1(ml) = 36.29 $\times$ 신장(cm) + 7.74 $\times$ 체중 잔차 - 3200.94 이었다. 이렇게 얻어진 예측값들의 설명력(R2)은 남자에서 FVC, FEV1가 각각 0.708, 0.670이었고, 여자에서는 FVC, FEV1가 각각 0.580, 0.513이었다.

• 대한의용생체공학회:의공학회지
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• 제22권5호
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• pp.449-458
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• 2001

한국형 양심실 보조 인공 심장의 효율 측정 방법과 측정 결과이다. 한국형 양심실 보조 인공 심장은 에너지 변환기로 브러시 없는 직류 모터를 사용한다 이 브러시 없는 직류 모터가 기어 열을 따라 원추운동을 하며 혈액주머니에 힘을 가하게 되고. 이 힘에 의해 혈액주머니 안의 혈액이 박출하게 된다. 효율 측정을 위하여 제어기 및 모터부, 기어부 및 작동기부. 혈액주머니 및 기타부 등 크게 세 부분으로 나누었으며. 각각을 다시 소자별로 나누어 입. 출력의 효율을 측정했다. 부하의 토크, 분당 회전수 각속도, 인가되는 전압 등을 바꾸어 가면서 시스템의 효율을 측정했고. 이 측정을 통해서 모터 운동시의 전체적인 효율과 부분별 효율을 알 수 있었다. 특히 양심실 보조 장치의 모터부와 작동기부의 효율측정에 중점을 두었다. 측정 결과 이동 작동기형 양심실 보조 장치의 평균 동작 영역인 4$\ell/min$ 박출량에서, 전체 효율은 8 %정도이며 구체적으로는 모터부 50 %, 작동기부 85 % 혈액주머니부 19 %정도의 효율을 보였다. 또한 6$\ell/min$ 박출량의 경우, 전체 효율은 5.5 %정도였다. 분석된 결과를 바탕으로 효율을 개선하기 위해 박출량에 따른 필요 구동 전압을 결정하고. 혈액주머니의 충만 상태에 따른 속도 파형을 연구. 제안하였다.

• Journal of Acupuncture Research
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• 제24권3호
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• pp.145-161
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• 2007

Objectives : The purpose of this study is to investigate the effect of electroacupuncture at Joksanmi($ST_{36}$) on Rat's aging by experimental methods. Methods : The author performed several experimental procedures to observe the effects of the EC-HAS at the arthritis. First, I measured the cell survival rate of the mice lung fibroblasts. Second, the incidence rate of arthritis and arthritis index of CIA were analyzed. Third, the levels of IL-6, $INF-{\gamma}$ , $INF-{\alpha}$ , $IL-1{\beta}$, IgG, IgM and anti-collagen II were measured in serum and the level of $INF-{\gamma}$, $INF-{\gamma}$/IL-4 ratio in the CIA mouse spleen cell culture. Fourth, histological analysis of the mice joint was performed. Fifth, the expression ratio of $CD3e^+$ cells to $CD19^+$ cells, $CD4^+$ cells to $CD8^+$ cells, $CD11a^+CD19^+$ cells, $CD4^+CD25^+$ cells, $CD3^+CD69^+$ cells, and $CD11b^+Gr-1^+$ cells were checked. Results: 1. The hematocrit was meaningfully (p<0.01, p<0.05) decreased in $ST_{36}$ group in comparison with the control and sham group. 2. The glucose, ALP were meaningfully (p<0.05, p<0.05) decreased in $ST_{36}$ group in comparison with the control group. 3. In the activation of hepatic antioxidase, the catalase was meaningfully (p<0.01) increased in $ST_{36}$ group in comparison with the control group. 4. In the liver, the NO formation was meaningfully (p<0.001) decreased in $ST_{36}$ group in comparison with the nomal, control and sham group. 5. In the activation of splenetic antioxidase, the GSH was meaningfully (p<0.01) increased in $ST_{36}$ group in comparison with the sham group, and the catalase was meaningfully (p<0.001) increased in $ST_{36}$ group in comparison with the control group. 6. In the spleen, the MDA formation was meaningfully (p<0.01, p<0.05) decreased in $ST_{36}$ group in comparison with the control and sham group. 7. In the spleen cell culture, the levels of IL-6 was meaningfully (p<0.05) decreased in comparison with the sham group, $INF-{\gamma}$( was meaningfully (p<0.05) increased in comparison with the control group. Conclusions: According to the results, it is considered that electroacupuncture at Joksamni($ST_{36}$) is effective in increasing the activities of antioxidative enzyme and inhibiting lipid peroxides, and has an immunomodulatory reaction. In conclusion, it han an significant anti-aging effect and need more experimental study.