• 생명과학회지
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• 제12권6호
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• pp.688-693
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• 2002

Chaperone 분자는 세포 내에서 새로 합성된 polypeptides의 misfolding을 보호하는 역할을 가진다. 이런 chaperone 분자와의 공발현은 활성형 재조합 단백질의 생산을 증가를 기대할 수 있다. 본 연구에서는 E. cozi에서 B. macerans 유래 cyclodextrin glucanotransferase (CGTase)의 활성형 생산에 GroEL/ES chaperone과의 공발현의 효과에 대해 조사하였다. cgt와 groEL/ES 유전자출 발현하는 pTCGT1과 pGro7은 각각 T7 promoter와 araB promoter에 의해 조절되고 이들을 E. coli cell에 co-transformation시켰다. 재조합 E. coli에서 IPTG와 L-arabinose의 최적 농도를 결정하기 위해 행한 결과 1 mM IPTG, 0.3 mg L-arabinose/$m\ell$에서 가장 높은 CGTase 활성을 나타내었다. 그리고 tube에서는 L-arabinose와 IPTG를 각각 0.4~0.5 $OD_{600}$과 0.8~l.0 $OD_{600}$에서 첨가하였을 때 활성형 CGTase의 생산이 증가되었다. GroEL/ES 공발현 조건에서는 가용성 CGTase 활성이 0.7~0.73 unit/$m\ell$로 단독 발현의 0.36~0.56 unit/$m\ell$에 비해 약 1.5 배 정도 증가함을 알 수 있었다. SDS-PAGE 분석에서는 GroEL/ES 공발현 조건에서 총 CGTase의 33.6%정도가 가용성 형태로 생산됨을 알 수 있었다.

• 산업식품공학
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• 제15권4호
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• pp.289-296
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• 2011

Twenty four fractions by solvent extraction and/or acid precipitation from fruit body and culture broth of Inonotus obliquus were prepared, and their inhibitory effect against acetylcholinesterase (AChE) was investigated. Among these fractions, acid (1 M HCl) precipitates from cell-free culture broth and fruit body exhibited the highest inhibitory effect on AChE in vitro. Acid precipitates inhibited AChE activity in a concentration-dependant manner and $IC_{50}$ values of both acid precipitates were 0.53 mg/mL. The inhibition pattern was general non-competitive inhibition. The energetic parameters were also determined by dual substrate/temperature design. Both acid precipitates increased the values of Ea, ${\Delta}H,/;{\Delta}G$ and ${\Delta}H^{\ast}$ decreasing the value of ${\Delta}S$ for AChE. The results implied that the acid precipitates from I. obliquus increased the thermodynamic barrier, leading to the breakdown of ES complex and the formation of products as inhibitory mechanism.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2022년도 학술발표회
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• pp.383-383
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• 2022

기존 큰 지진을 대상으로 한 많은 연구결과들을 살펴보면, 큰 지진이 발생하기 전에 토양 속에 존재하는 다양한 인자들 중에 라돈(²²²Rn, 반감기=3.82일) 농도가 비정상적으로 증가하는 현상을 나타낸다. 이러한 결과들은 라돈발생의 경향성을 분석한다면, 지진발생에 대한 전조증상이나 예측이 가능함을 나타낸다. 본 연구에서는 포항지역을 중심으로 지진발생에 대한 전조증상이나 모니터링을 분석하기 위해 지하수 관측소에 설치된 라돈 관측기기에서 라돈 관측자료를 수집하고 이를 활용하는 연구를 수행하였다. 라돈 관측자료 기간은 2019년 11월부터 2020년 9월까지의 자료이며, 라돈인자 뿐만 아니라 지하수위, 강수량, 수온인자를 같이 분석하였으며, 동일기간 동안 발생한 진도 2 이상의 지진사례 6개(E1(M_L 3.5): 2019.12.30.; E2(M_L 3.2): 2020.01.30.; E3(M_L 2.4): 2020.02.09.; E4(M_L 2.7): 2020.02.16.; E5(M_L 2.8): 2020.05.27.; E6(M_L 2.1): 2020.09.22.)를 대상으로 하였다. 지진발생의 전조증상이 나타나는 지역을 분석하기 위해 Dobrovolsky radius values (Dobrovolsky et al., 1979)와 Harversine 관계식을 적용하였다. 적용결과, 시간적 분석에서 라돈의 증감 경향성이 지진발생의 전조증상과 유의미한 상관성이 있음을 확인하였으며, 공간적인 분석에서도 유의미하지는 않으나 상관성이 나타났다. 그 외 지하수위는 상관성이 어느 정도 나타났으나 강수량은 유의미한 결과를 나타내지는 않았다. 따라서 라돈을 활용한다면 지진발생의 전조증상을 시공간적으로 파악할 수 있음을 확인하였다.

• Journal of Power Electronics
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• 제19권2호
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• pp.540-548
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• 2019

In this work a new configuration of E-core stator Switched Reluctance Motor (SRM) with permanent magnets and auxiliary windings embedded in the stator yoke is proposed. For the proposed configuration of SRM electromagnetic analysis is performed using Finite Element Analysis (FEA) based computer aided design package MagNet and to emphasize its merits a comparison is drawn with existing hybrid excitation configuration of SRM. In addition, the vibration characteristics of the motor are analyzed by performing modal and transient analysis using the ANSYS package. Results of the analysis reveals that the proposed configuration of SRM exhibits better electromagnetic and vibration characteristics and is capable of competing with the existing topologies in the variable speed market.

• Archives of Pharmacal Research
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• 제29권2호
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• pp.145-151
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• 2006

We investigated the effects of Ginsenoside $R_e$ on human sperm motility in fertile and asthenozoospermic infertile individuals in vitro and the mechanism by which the Ginsenosides play their roles. The semen samples were obtained from 10 fertile volunteers and 10 asthenozoospermic infertile patients. Spermatozoa were separated by Percoll and incubated with 0, 1, 10 or $100\;{\mu}M$ of Ginsenoside $R_e$. Total sperm motility and progressive motility were measured by computer-aided sperm analyzer (CASA). Nitric oxide synthase (NOS) activity was determined by the $^{3}H$-arginine to $^{3}H$-citrulline conversion assay, and the NOS protein was examined by the Western blot analysis. The production of sperm nitric oxide (NO) was detected using the Griess reaction. The results showed that Ginsenoside $R_e$ significantly enhanced both fertile and infertile sperm motility, NOS activity and NO production in a concentration-dependent manner. Sodium nitroprusside (SNP, 100 nM), a NO donor, mimicked the effects of Ginsenoside $R_e$. And pretreatment with a NOS inhibitor $N^{w}$-Nitro-L-arginine methyl ester (L-NAME, $100\;{\mu}M$) or a NO scavenger N-Acetyl-L-cysteine (LNAC, 1 mM) completely blocked the effects of Ginsenoside $R_e$. Data suggested that Ginsenoside $R_e$ is beneficial to sperm motility, and that induction of NOS to increase NO production may be involved in this benefit.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.245-250
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• 2006

The effects of aliphatic hydrocarbons (n-hexadecane and n-dodecane) on the volumetric oxygen mass transfer coefficient $(k_L\;a)$ were studied in flat alveolar airlift reactor and continuous stirred tank reactors (CSTRs). In the flat alveolar airlift reactor, high aeration rates (>2vvm) were required in order to obtain efficient organic-aqueous phase dispersion and reliable $k_La$ measurements. Addition of 1% (v/v) n-hexadecane or n-dodecane increased the $k_La$ 1.55- and 1.33-fold, respectively, compared to the control (superficial velocity: $25.8{\times}10^{-3}m/s$, sparger orifice diameter: 0.5 mm). Analysis of the gas-liquid interfacial area a and the liquid film mass transfer coefficient $k_L$ suggests that the observed $k_La$ increase was a function of the media's liquid film mass transfer. Addition of 1% (v/v) n-hexadecane or n-dodecane to analogous setups using CSTRs led to a $k_La$ increase by a factor of 1.68 and 1.36, respectively (superficial velocity: $2.1{\times}10^{-3}m/s$, stirring rate: 250 rpm). These results propose that low-concentration addition of oxygen-vectors to aerobic microbial cultures has additional benefit relative to incubation in purely aqueous media.

• 소음진동
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• 제4권4호
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• pp.443-449
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• 1994

In the analysis of circular cylindrical shell's vibration and sound radiation, there are numerical and analytical methods. Numerical methods such as F.E.M and B.E.M, have the limit of frequency range. Analytical method can be applied to the circular cylindrical shell from low frequency to high frequency. In this paper, we use the analytical method for shell, and numerical method, F.D.M, for fluid. We also use the method using transfer matrix and eigenanalysis of transfer matrix which can therefore calculate the rotational d.o.f that is very imkportant in synthesis with inner structure. Inner structure has much effect on the submerged circular cylindrical shell vibration and sound rediation. Results for the immersed circular cylindrical shell vibration and sound radiation are compared with the analytic solutions.

• Genomics & Informatics
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• 제16권4호
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• pp.38.1-38.3
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• 2018

Gene-gene interaction (GGI) analysis is known to play an important role in explaining missing heritability. Many previous studies have already proposed software to analyze GGI, but most methods focus on a binary phenotype in a case-control design. In this study, we developed "Hierarchical structural CoMponent analysis of Gene-Gene Interactions" (HisCoM-GGI) software for GGI analysis with a continuous phenotype. The HisCoM-GGI method considers hierarchical structural relationships between genes and single nucleotide polymorphisms (SNPs), enabling both gene-level and SNP-level interaction analysis in a single model. Furthermore, this software accepts various types of genomic data and supports data management and multithreading to improve the efficiency of genome-wide association study data analysis. We expect that HisCoM-GGI software will provide advanced accessibility to researchers in genetic interaction studies and a more effective way to understand biological mechanisms of complex diseases.

• 한국농공학회지
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• 제38권2호
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• pp.75-86
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• 1996

The formulas for estimating the constants of storage function model including K and TL for runoff analysis and a distributed storage function model are discussed in this study. First, the relations between parameters of the storage function model and the kinematic runoff model are theoretically examined, and then optimum constants of storage function model are obtained by the Standardized Davidson-Fletcher-Powell (SDFP) method. Through this analysis, theoretical formulas were obtained as $K = 0.63 {\alpha} KsB{^0.6}$ and $T_{L}=0.11 {\alpha} KsB{^0.6} r{^0.4} {_e}$, which are difficult to use practically because of the unclarified definition of shape factors. From a practical point of view, empirical formula were derived as $K=15.6{^0.3} {_m}$ and $T_{L}=2.1B{^0.36} {_m} {_e}/r{^0.4} {_e}$ for applied watersheds. The proposed formulas are verified for several recoded floods at a few points of watersheds. It is also found that the distributed storage function. can be applied to flood runoff analysis using the new formulas aboved mentioned.

• 전기의세계
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• 제29권9호
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• pp.599-603
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• 1980

Mathematically, the Electromagnetic phenomena can be represented by the Maxwell's equations, but it is very difficult to solve these equations, especially, having complex structural boundaries. By the way, the development of a computer system made us easy to solve these kind of partial differential equations. The Finite Element Method, one of the numerical methods, is very this. This paper shows the power of F.E.M. by examining, with an example of a hollow cylinder in a uniform magnetic field which is analytically solvable, the errors and the tendency of magnetic flux lines.