• Journal of the Korean Magnetic Resonance Society
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• v.24 no.4
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• pp.136-142
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• 2020

Apolipoprotein B-100 (apo B-100), the main protein component that makes up LDL (Low density lipoprotein), consists of 4,536 amino acids and serves to combine with the LDL receptor. The oxidized LDL peptides by malondialdehyde (MDA) or acetylation in vivo were act as immunoglobulin (Ig) antigens and peptide groups were classified into 7 peptide groups with subsequent 20 amino acids (P1-P302). The biomimetic peptide P99 (KGTYG LSCQR DPNTG RLNGE) out of B-group peptides carrying the highest value of IgM antigens were selected for structural studies that may provide antigen specificity. Circular Dichroism (CD) spectra were measured for peptide secondary structure in the range of 190-260 nm. Experimental results show that P99 has pseudo α-helice and random coil structure. Homonuclear (COSY, TOCSY, NOESY) 2D-NMR experiments were carried out for NMR signal assignments and structure determination for P99. On the basis of these completely assigned NMR spectra and proton distance information, distance geometry (DG) and molecular dynamic (MD) were carried out to determine the structures of P99. The proposed structure was selected by comparisons between experimental NOE spectra and back-calculated 2D NOE results from determined structure showing acceptable agreement. The total Root-Mean-Square-Deviation (RMSD) value of P99 obtained upon superposition of all atoms were in the set range. The solution state P99 has mixed structure of pseudo α-helix and β-turn(Gln[9] to Thr[13]). These NMR results are well consistent with secondary structure from experimental results of circular dichroism. Structural studies based on NMR may contribute to the prevent oxidation studies of atherosclerosis and observed conformational characteristics of apo B-100 in LDL using monoclonal antibodies.

• Journal of the Korean Magnetic Resonance Society
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• v.25 no.4
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• pp.58-63
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• 2021

Apolipoprotein B-100 (apo B-100), the main protein component that makes up LDL (Low density lipoprotein), consists of 4,536 amino acids and serves to combine with the LDL receptor. The oxidized LDL peptides by malondialdehyde (MDA) or acetylation in vivo act as immunoglobulin (Ig) antigens and peptide groups were classified into 7 peptide groups with subsequent 20 amino acids (P1-P302). The biomimetic peptide P143 (IALDD AKINF NEKLS QLQTY) out of C-group peptides carrying the highest value of IgG antigens were selected for structural studies that may provide antigen specificity. Experimental results show that P143 has β-sheet in Ile[1]-Asn[9] and α-helice in Gln[16]-Tyr[20] structure. Homonuclear 2D-NMR (COSY, TOCSY, NOESY) experiments were carried out for NMR signal assignments and structure determination for P143. On the basis of these completely assigned NMR spectra and proton distance information, distance geometry (DG) and molecular dynamic (MD) were carried out to determine the structures of P143. The proposed structure was selected by comparisons between experimental NOE spectra and back-calculated 2D NOE results from determined structure showing acceptable agreement. The total Root-Mean-Square-Deviation (RMSD) value of P143 obtained upon superposition of all atoms were in the set range. The solution state P143 has a mixed structure of pseudo α-helix and β-turn(Phe[10] to Glu[12]). These results are well consistent with calculated structure from experimental data of NOE spectra. Structural studies based on NMR may contribute to the prevent oxidation studies of atherosclerosis and observed conformational characteristics of apo B-100 in LDL using monoclonal antibodies.

• Structural Engineering and Mechanics
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• v.91 no.1
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• pp.75-86
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• 2024

In this paper, the porous metal PM-35 is proposed as the filler material of filled thin-walled tubes (FTTs), and a series of experimental study is conducted to investigate the dynamic behavior and energy absorption performance of PM-35 filled thin-walled tubes under impact loading. Firstly, cylinder solid specimens of PM-35 steel are tested to investigate the impact mechanical behavior by using the Split Hopkinson pressure bar set (SHP); Secondly, the filled thin-walled tube specimens with different geometric parameters are designed and tested to investigate the feasibility of PM-35 steel applied in FTTs by the orthogonal test. According to the results of this research, it is concluded that PM-35 steel is with the excellent characteristics of high energy absorption capacity and low yield strength, which make it a potential filler material for FTTs. The micron-sizes pore structure of PM-35 is the main reason for the macroscopic mechanical behavior of PM-35 steel under impact loading, which makes the material to exhibit greater deformation when subjected to external forces and obviously improve the toughness of the material. In addition, PM-35 steel core-filled thin-wall tube has excellent energy absorption ability under high-speed impact, which shows great application potential in the anti-collision structure facilities of high-speed railway and maglev train. The parameter V0 is most sensitive to the energy absorption of FTT specimens under impact loading, and the sensitivity order of different variations to the energy absorption is loading speed V0>D/t>D/L. The loading efficiency of the FTT is affected by its different geometry, which is mainly determined by the sleeve material and the filling material, which are not sensitive to changes in loading speed V0, D/t and D/L parameters.

• Journal of Welding and Joining
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• v.30 no.1
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• pp.59-63
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• 2012

The spacer grid is one of the main structural components in a fuel assembly. It supports fuel rods, guides cooling water and maintains geometry from external impact load and cyclic stress by the vibration of nuclear fuel rod, it is necessary to have sufficient strength against dynamic external load and fatigue strength. In this study, the mechanical properties and fatigue characteristics of laser beam welded zircaloy thin sheet are examined. The material used in this study is a zirconium alloy with 0.66 mm of thickness. The fatigue strength under cyclic load was evaluated at stress ratio R=0.1. S-N curves are presented with statistical testing method recommend by JSME- S002 and compared with S-N curves at R.T. and $315^{\circ}C$. As a result of the experimental approach, the design guide of fatigue strength is proposed and the results obtained from this study are expected to be useful data for spacer gird design.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1989.04a
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• pp.38-43
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• 1989

An improved model for predicting the reinforced concrete element behavior under dynamic strain rates was developed using the layer modeling technique. The developed strain rate sensitive model for axial/flexural analysis of reinforced concrete elements was uses to predict the test results, performed at different loading rates, and the predictions were reasonable. The developed analysis technique was used to study the loading rate sensitivity of reinforced concrete beams and columns with different geometry and material properties. Two design formulas for computing the loading rate dependent axial and flexural strengths of reinforced concrete sections were also suggested.

• East Asian mathematical journal
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• v.30 no.2
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• pp.149-172
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• 2014

The problems of optimization addressed in the high school curriculum are usually posed in real-life contexts. However, because of the instructional purposes, problems are artificially constructed to suit computation, rather than to reflect real-life problems. Those problems have thus limited use for teaching 'practicalities', which is one of the goals of mathematics education. This study, by utilizing 'GeoGebra', suggests the optimization problem solving related to the quadratic curve, using the contour-line method which contemplates the quadratic curve changes successively. By considering more realistic situations to supplement the limit which deals only with numerical and algebraic approach, this attempt will help students to be aware of the usefulness of mathematics, and to develop interests in mathematics, as well as foster students' integrated thinking abilities across units. And this allows students to experience a variety of math.

• International Journal of Naval Architecture and Ocean Engineering
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• v.10 no.3
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• pp.348-359
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• 2018

A series of indoor simulation tests on a large-sized shaking table was performed, which was used to simulate the earthquake ground motion for the pipe-soil interaction system to be tested. The purpose of this study is to examine the dynamic characteristic and seismic response of a length of PVC pipeline lay on a clay seabed under seismic load. The pipeline was fully instrumented to provide strain and acceleration responses in both transverse and in-line. Dynamical modal tests show that corresponding mode shapes vertically and horizontally are basically the same. But the absolute values of the natural frequencies vertically are all higher than those corresponding values in transverse. It turned out that the geometry configuration of riser affects its stiffness. Seismic response of pipeline depends significantly on the waveform, and Peak Ground Acceleration (PGA). As the seismic loading progressed, the strain response was severe around both TDZ and catenary zone. Additionally, strain responses in top and bottom positions were more severe than the result in left or right side of the pipeline in the same section.

• Earthquakes and Structures
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• v.12 no.3
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• pp.359-374
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• 2017

The seismic events in Northern Italy, May 2012, have revealed the seismic vulnerability of typical Italian precast industrial buildings. The aim of this paper is to present a seismic fragility model for Italian RC precast buildings, to be used in earthquake loss estimation and seismic risk assessment by comparing two building typologies and three different codes: D.M. 3-03-1975, D.M. 16-01-1996 and current Italian building code that has been released in 2008. Based on geometric characteristics and design procedure applied, ten different building classes were identified. A Monte Carlo simulation was performed for each building class in order to generate the building stock used for the development of fragility curves trough analytical method. The probabilistic distributions of geometry were mainly obtained from data collected from 650 field surveys, while the material properties were deduced from the code in place at the time of construction or from expert opinion. The structures were modelled in 2D frameworks; since the past seismic events have identified the beam-column connection as the weakest element of precast buildings, two different modelling solutions were adopted to develop fragility curves: a simple model with post processing required to detect connection collapse and an innovative modelling solution able to reproduce the real behaviour of the connection during the analysis. Fragility curves were derived using both nonlinear static and dynamic analysis.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.38 no.5
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• pp.375-381
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• 2001

As the device geometry continuously shrink down less than sub-quarter micrometer, DRAM makers are going to replace conventional tungsten-polycide bit-line with tungsten bit-line structure in order to reduce the chip size and use it as a local interconnection. In this paper we showed low resistance tungsten bit-line fabrication process with various RTP(Rapid Thermal Process) temperature and additional ion implantation. As a result we obtained that major parameters impact on tungsten bit-line process are RTP Anneal temperature and BF$_2$ ion implantation dopant. These tungsten bit-line process are promising to fabricate high density chip technology.

• Transactions of the Korean hydrogen and new energy society
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• v.31 no.3
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• pp.314-321
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• 2020

In this work, a preliminary design of an inlet guide vane and runner for developing a 2.5 kW hydraulic turbine was conducted by using computational fluid dynamic analysis. Three-dimensional Reynolds-averaged Navier-Stokes equations with shear stress transport turbulence model were used to analyze the fluid flow in the hydraulic turbine. The hexahedral grid system was used to construct computational domain, and the grid dependency test was performed to obtain the optimal grid system. Velocity triangle diagram considering the flow angles of the inlet guide vane and runner was analyzed to obtain a basic geometry of the inlet guide vane and runner. Through modification of the preliminary design, the hydraulic performances of the turbine have improved under overall drop conditions. Especially, the efficiency and power of the turbine increased by 0.95% and 1.45%, respectively, compared to those of the reference model.