• Title/Summary/Keyword: Dye-Function

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Equilibrium, Kinetics and Thermodynamics Studies about Adsorption of Safranin by Granular Activated Carbon (입상 활성탄에 의한 Safranin의 흡착에 관한 평형, 동력학 및 열역학에 관한 연구)

  • Lee, Jong Jib
    • Applied Chemistry for Engineering
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    • v.26 no.5
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    • pp.581-586
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    • 2015
  • Adsorption of Safranin using granular activated carbon from aqueous solution was investigated. Batch experiments were carried out as a function of adsorbent dose, initial concentration, contact time and temperature. The equilibrium adsorption data were fitted to Langmuir, Freundlich and Dubinin-Radushkevich isotherm models. Based on an estimated Langmuir separation factor, $R_L=0.183{\sim}0.254$ and a Freundlich separation factor, 1/n = 0.518~0.547, this process could be employed as an effective treatment method. Adsorption data were also modeled using the pseudo-first and second-order kinetic equations. It was shown that the pseudo-second-order kinetic equation could best describe the adsorption kinetics. The negative Gibbs free energy (${\Delta}G=-3.688{\sim}-7.220kJ/mol$) and positive enthalpy (${\Delta}H=33.126kJ/mol$) indicated that the adsorption process was spontaneous and endothermic.

The Molecular Biological Study on Anti-Cancer Effects of Sagunjatang plus Cremastrae Appenediculatae Tuber on Human Stomach Cancer Cells (사군자탕가산자고가 위암세포에 미치는 항암효과에 대한 분자생물학적 연구)

  • Ryu, Bong-Ha;Ryu, Ki-Won;Yoon, Sang-Hyub;Kim, Jin-Seong;Kim, Jin-Seok
    • The Journal of Internal Korean Medicine
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    • v.23 no.2
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    • pp.202-211
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    • 2002
  • 1. Background The previous studies on anticancer medicine derived from korean traditional medicine have focused on the life elongation of cancer cell bearing animals. However, it is thought that more molecular biological studies are needed to reveal their mechanism. 2. Objective The aim of this study was to investigate the molecular biological function of Sagunjatang plus Cremastrae Appenediculatae Tuber on cytostaticity, apoptosis and apoptosis related genes revelation against human stomach cancer cells(AGS). 3. Methods After administrating Sagunjatang and Sagunjatang plus Cremastrae Appenediculatae Tuber to human stomach cancer cell. MTT assay was performed to compare and examine the efficacy of each medicine on the cytostaticity of stomach cancer cells in proportion to time and doses, and apoptosis assay was performed to examine their effect on apoptosis by using DAPI dye and counting the number of cells which developed in an apoptotic body. In addition, the quantitative RT-PCR was used to examine their effect on the revelation of Bcl-2, Bax and P53, which are genes related to apoptosis. 4. Result and Conclusion Sagunjatang plus Cremastrae Appenediculatae Tuber demonstrated increased cytostaticity. decreased apoptosis and unremarkable revelation of apoptosis related genes. But in the cytostaticity and apoptosis, Sagunjatang plus Cremastrae Appenediculatae Tuber showed a tendency to control stomach cancer cells. Therefore, we can expect the clinical application to the related diseases. Besides, it needs another experiment on various cancer cells, such as, lung cancer cell and hysterocarcinoma cell.

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Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

  • Kim, Su-Nam;Cho, Won-Kyong;Kim, Won-Il;Jee, Hyeong-Jin;Park, Chang-Seuk
    • The Plant Pathology Journal
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    • v.28 no.3
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    • pp.270-281
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    • 2012
  • The bacteria B1-9 that was isolated from the rhizosphere of the green onion could promote growth of pepper, cucumber, tomato, and melon plants. In particular, pepper yield after B1-9 treatment on the seedling was increased about 3 times higher than that of control plants in a field experiment. Partial 16S rDNA sequences revealed that B1-9 belongs to the genus Pantoea ananatis. Pathogenecity tests showed non-pathogenic on kimchi cabbage, carrot, and onion. The functional characterization study demonstrated B1-9's ability to function in phosphate solubilization, sulfur oxidation, nitrogen fixation, and indole-3-acetic acid production. To trace colonization patterns of B1-9 in pepper plant tissues, we used $DRAQ5^{TM}$ fluorescent dye, which stains the DNAs of bacteria and plant cells. A large number of B1-9 cells were found on the surfaces of roots and stems as well as in guard cells. Furthermore, several colonized B1-9 cells resided in inner cortical plant cells. Treatment of rhizosphere regions with strain B1-9 can result in efficient colonization of plants and promote plant growth from the seedling to mature plant stage. In summary, strain B1-9 can be successfully applied in the pepper plantation because of its high colonization capacity in plant tissues, as well as properties that promote efficient plant growth.

Flow Lab. : Flow Visualization and Simulation (핵종이동 가시적 현상관찰및 수치모사)

  • Park Chung-Kyun;Cho Won-Jin;Hahn Pi1-Soo
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2005.11a
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    • pp.134-142
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    • 2005
  • The experimental setups for flow visualization and processes identification in laboratory scale (so cal led Flow Lab.) has developed to get ideas and answer fundamental questions of flow and migration in geologic media. The setup was made of a granite block of $50{\times}50cm$ scale and a transparent acrylate plate. The tracers used in this experiments were tritiated water, anions, and sorbing cations as well as an organic dye, eosine, to visualize migration paths. The migration plumes were taken with a digital camera as a function of time and stored as digital images. A migration model was also developed to describe and identify the transport processes. Computer simulation was carried out not only for the hydraulic behavior such as distributions of pressure and flow vectors in the fracture but also for the migration plume and the elution curves.

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The Concentration-Dependent Distribution of Tris(4,7'-diphenyl-1,10'-phenanthroline) Ruthenium (II) within Sol-Gel-Derived Thin Films

  • Lee, Joo-Woon;Cho, Eun-Jeong
    • Bulletin of the Korean Chemical Society
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    • v.32 no.8
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    • pp.2765-2770
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    • 2011
  • Organic dye-doped glasses, viz., ruthenium (II) tris(4,7'-diphenyl-1,10'-phenanthroline) $[Ru(dpp)_3]^{2+}$ incorporated into thin silica xerogel films produced by the sol-gel method, were prepared and their $O_2$ quenching properties investigated as a function of the $[Ru(dpp)_3]^{2+}$ concentration (3-400 ${\mu}M$) within the xerogel. The ratio of the luminescence from the $[Ru(dpp)_3]^{2+}$-doped films in the presence of $N_2$ and $O_2$ ($I_{N2}/I_{O2}$) was used to describe the film sensitivity to $O_2$ quenching. ($I_{N2}/I_{O2}$ changed three-fold over the $[Ru(dpp)_3]^{2+}$ concentration range. Time-resolved intensity decay studies showed that there are two discrete $[Ru(dpp)_3]^{2+}$ populations within the xerogels (${\tau}_1$ ~ 300 ns; ${\tau}_2$ ~ 3000 ns) whose relative fraction changes as the $[Ru(dpp)_3]^{2+}$ concentration changes. The increased $O_2$ sensitivity that is observed at the higher $[Ru(dpp)_3]^{2+}$ concentrations is a manifestation of a greater fraction of the 3000 ns $[Ru(dpp)_3]^{2+}$ species (more susceptible to $O_2$ quenching). A model is presented to describe the observed response characteristics resulting from $[Ru(dpp)_3]^{2+}$ distribution within the xerogel.

Effectiveness of Feather Waste for Orange II Removal from Aqueous Solutions (수용액 중 Orange II 흡착 제거를 위한 우모폐기물의 이용가능성)

  • Park, Soo-Yeun;Yoo, Ji-Yeon;Son, Hong-Joo
    • Journal of Environmental Science International
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    • v.27 no.9
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    • pp.743-751
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    • 2018
  • The objective of this study was to investigate the adsorption potential of chicken feathers for the removal of OrangeII (AO7) from aqueous solutions. Batch experiments were performed as a function of different experimental parameters such as initial pH, reaction time, feather dose, initial OrangeII concentration and temperature. The highest OrangeII uptake was observed at pH 1.0. Most of the OrangeII was adsorbed at 2 h and an adsorption equilibrium was reached at 6 h. As the amount of chicken feather was increased, the removal efficiency of OrangeII increased up to 99%, but its uptake decreased. By increasing the initial concentration and temperature, OrangeII uptake was increased. The experimental adsorption isotherm exhibited a better fit with the Langmuir isotherm than with the Freundlich isotherm, and maximum adsorption capacity from the Langmuir constant was determined to be 0.179244 mmol/g at $30^{\circ}C$. The adsorption energy obtained from the Dubinin-Radushkevich model was 7.9 kJ/mol at $20^{\circ}C$ and $30^{\circ}C$ which indicates the predominance of physical adsorption. Thermodynamic parameters such as ${\Delta}G^0$, ${\Delta}H^0$, and ${\Delta}S^0$ were -12.28 kJ/mol, 20.64 kJ/mol and 112.32 J/mol K at $30^{\circ}C$, respectively. This indicates that the process of OrangeII adsorption by chicken feathers was spontaneous and endothermic. Our results suggest that as a low-cost biomaterials, chicken feather is an attractive candidate for OrangeII removal from aqueous solutions.

A Literature Review on Cracked Teeth (균열치 증후군에 대한 문헌고찰)

  • Cho, Woong-Rae;Kim, Dae-Gon;Park, Chan-Jin;Cho, Lee-Ra
    • Journal of Dental Rehabilitation and Applied Science
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    • v.27 no.3
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    • pp.305-316
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    • 2011
  • Cracked teeth are a common problem with incomplete fracture that typically shows pain on biting and temperature stimuli. The most common cause of crack is known to be the masticatory accident. Predisposing factors were aging, old restoration, and iatrogenic stress concentration, etc. Accurate diagnosis is needed for detecting the extent of crack and pulp vitality. There are many diagnostic tools like bite test, direct observation with transillumination, dye penetration, and radiographs. Immediate treatment for pain relief and occlusal adjustment would be needed. Composite resin and bonded amalgam showed favorable prognosis. Moreover, cusp protection is reliable permanent treatment for cracked teeth. The purpose of this article is to review the literature about the prevention, diagnosis, and treatment for cracked teeth.

Adsorption Kinetic, Thermodynamic Parameter and Isosteric Heat for Adsorption of Crystal Violet by Activated Carbon (활성탄에 의한 Crystal Violet 흡착에 있어서 흡착동력학, 열역학 인자 및 등량흡착열)

  • Lee, Jong Jib
    • Applied Chemistry for Engineering
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    • v.28 no.2
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    • pp.206-213
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    • 2017
  • The adsorption of crystal violet dyes from aqueous solution using the granular activated carbon was investigated. Adsorption experiments were carried out as a function of the adsorbent dose, initial concentration, contact time and temperature. The adsorption characteristic of crystal violet followed Langmuir isotherm. Based on the estimated Langmuir separation factor ($R_L=0.02{\sim}0.106$), this process could be employed as an effective treatment (0 < $R_L$ < 1). The adsorption kinetics followed the pseudo second order model. The values of Gibbs free energy (-1.61~-11.66 kJ/mol) and positive enthalpy (147.209 kJ/mol) indicated that the adsorption process is a spontaneous and endothermic reaction. The isosteric heat of adsorption decreased with increasing of surface loading by the limited adsorbent-adsorbate interaction due to increased surface coverage.

Application of the Response Surface Methodology and Process Optimization to the Electrochemical Degradation of Rhodamine B and N, N-Dimethyl-4-nitrosoanilin Using a Boron-doped Diamond Electrode (Boron-doped Diamond 전극을 이용한 Rhodamine B와 N, N-Dimethyl-4-nitrosoanilin의 전기화학적 분해에 반응표면분석법의 적용과 공정 최적화)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Health Sciences
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    • v.36 no.4
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    • pp.313-322
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    • 2010
  • The aim of this research was to apply experimental design methodology to optimization of conditions of electrochemical oxidation of Rhodamine B (RhB) and N, N-Dimethyl-4-nitrosoaniline (RNO, indicative of the OH radical). The reactions of electrochemical oxidation of RhB degradation were mathematically described as a function of the parameters of current ($X_1$), NaCl dosage ($X_2$) and pH ($X_3$) and modeled by the use of the central composite design. The application of response surface methodology (RSM) yielded the following regression equation, which is an empirical relationship between the removal efficiency of RhB and RNO and test variables in a coded unit: RhB removal efficiency (%) = $94.21+7.02X_1+10.94X_2-16.06X_3+3.70X_1X_3+9.05X_2X_3-{3.46X_1}^2-{4.67X_2}^2-{7.09X_3}^2$; RNO removal efficiency (%) = $54.78+13.33X_1+14.93X_2- 16.90X_3$. The model predictions agreed well with the experimentally observed result. Graphical response surface and contour plots were used to locate the optimum point. The estimated ridge of maximum response and optimal conditions for the RhB degradation using canonical analysis was 100.0%(current, 0.80 A; NaCl dosage, 2.97% and pH 6.37).

Arthroscopic Treatment for Meniscal Cyst (관절경을 이용한 반월상연골 낭종의 치료)

  • Min, Byoung-Hyun;Lee, Weon Ik;Choi, Seung Joon;Kang, Shin Young
    • Journal of the Korean Arthroscopy Society
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    • v.2 no.2
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    • pp.141-146
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    • 1998
  • Recent studies suggest that most meniscal cysts can be treated surgically by arthroscopic management of the meniscal tear and arthroscopic cyst evacuation. But arthroscopic cyst decompression may sacrifice a substantial amount of meniscal tissue that is not torn in order to expose the "stalk" of the cyst. Nowadays, the trend is changing as preserving the involved meniscus to prevent from inevitable degenerative changes after meniscectomy. The purpose of this report is to describe a new surgical technique that minimizes loss of meniscal tissue in hopes of maximizing residual meniscal function. We experienced 10 patients with meniscal cysts that were consisted of four lateral cysts and six medial cysts. Menisci were torn in all cases. Arthroscopic partial meniscectomy and decompression of cysts were performed in 9 cases, and arthroscopic partial meniscectomy and open cystectomy in 1 case. The procedures were consisted of injection of the methylene blue into the cyst, partial meniscectomy of the meniscal tear until the dye was seen in orifice of the cyst, and decompression of cyst through cystic opening. This article serves to confirm the relationship between torn menisci and cysts, to re-evaluate the occurrence ratio of the meniscal cyst on the medial to lateral meniscus, and to assess the efficacy of arthroscopic partial meniscectomy and decompression of cyst as a potentially meniscal sparing procedure.

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