• 제목/요약/키워드: Dye

검색결과 3,901건 처리시간 0.036초

Fluorescence Quenching Causes Systematic Dye Bias in Microarray Experiments Using Cyanine Dye

  • Jeon, Ho-Sang;Choi, Sang-Dun
    • Genomics & Informatics
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    • 제5권3호
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    • pp.113-117
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    • 2007
  • The development of microarray technology has facilitated the understanding of gene expression profiles. Despite its convenience, the cause of dye-bias that confounds data interpretation in dual-color DNA microarray experiments is not well known. In order to economize time and money, it is necessary to identify the cause of dye bias, since designing dye-swaps to reduce the dye-specific bias tends to be very expensive. Hence, we sought to determine the reliable cause of systematic dye bias after treating murine macrophage RAW 264.7 cells with 2-keto-3-deoxyoctonate (KDO), interferon-beta $(IFN-{\beta})$, and 8-bromoadenosine (8-BR). To find the cause of systematic dye bias from the point of view of fluorescence quenching, we examined the correlation between systematic dye bias and the proportion of each nucleotide in mRNA and oligonucleotide probe sequence. Cy3-dye bias was highly correlated with the proportion of adenines. Our results support the fact that systematic dye bias is affected by fluorescence quenching of each feature. In addition, we also found that the strength of fluorescence quenching is based on not only dye-dye interactions but also dye-nucleotide interactions as well.

나일론/카티온화 면 혼방품의 산성염료/직접염료에 의한 1욕 2단 염색에 관한 연구 (A Study on the One Bath Two Step Dyeing of Nylon/ Cationized Cotton Blends with Acid Dye/Direct Dye)

  • 성우경
    • 한국염색가공학회지
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    • 제12권1호
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    • pp.44-51
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    • 2000
  • The conventional one bath exhaust dyeing system of nylon]/cotton blends with acid dye/direct dye involves a lot of neutral salt which affect dyeability of acid dyes. Because of conventional one bath exhaust dyeing system of nylonl/cotton blends with acid dye/direct dye needs acid dyes adjusted at a neutral liquor, suitable acid dye is limited. To improve dyeing property of nylon/cotton blends, cotton component was pretreated with cationizing agent containing chlorohydrine group in aqueous solution of sodium hydroxide. This study was carried out to investigate dyeing possibilities of nylon/cationized cotton blends with acid dye/direct dye in a non-neutral salt dyebath by one bath two step method. The concentration of direct dye was 1.0%, 0.7%, 0.3%o.w.f. respectively at a non, 1%, 3%owf of reserving agent for being almost equal color strength between nylon and 3% cationized cotton when nylon/3% cationized cotton blends was dyed with acid dye/direct dye at concentration of acid dye(1%o.w.f.) by one bath two step method.

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Dye-resist Properties of Hetero-multifunctional Dye-resist Agents in Acid Dyeing of Wool

  • Oh Myung-Joon;Koh Joon-Seok;Kim Jae-Pil
    • Fibers and Polymers
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    • 제7권2호
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    • pp.117-122
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    • 2006
  • The dye-resist effect and leveling properties of hetero-mulifunctional dye-resist agents in acid dyeing of wool were investigated. The dye-resist agent with dichlorotriazinyl group achieved better resist effectiveness than those with monochlo-rotriazinyl group. The resist effectiveness was improved by increasing the number of sulfonate group in dye-resist agents. Also, the resist agents with more sulfonate groups showed better dye-assist effectivness, attributable to the increased electrostatic attraction between dye-resist agents and the cationic dye. However, the leveling properties of dye-resist agents decreased with the number of sulfonate groups in the molecule.

Dye-resist Properties of Reactive Dye-resist Agents in Reactive Dyeing of Silk

  • Park, Sung-Hee;Oh, Myung-Joon;Koh, Joon-Seok
    • 한국염색가공학회지
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    • 제19권5호
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    • pp.8-16
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    • 2007
  • The dye-resist effect of reactive dye-resist agents in reactive dyeing of silk was investigated. The dichlorotriazine-based dye-resists achieved a higher effectiveness than others since they make a charge barrier of diffusion in the silk fiber periphery due to high reactivity of dichlorotriazine group. Similarly, in the case of hetero-multifunctional dye-resist agent, the dye-resist agent containing both a dichlorotriazine and an ${\alpha}$-bromoacrylamide reactive groups achieved better resist effectiveness than those containing both a monochlorotriazine and an ${\alpha}$-bromoacrylamide groups. Also, their resist effectiveness was improved by increasing the number of sulfonate groups in the dye-resist agents and the number of reactive groups in the reactive dyes applied to them.

염료감응태양전지를 위한 $TiO_2$ 분말 기공도와 염료 흡착량의 관계 (Relationship between the porosity of the nanostructured $TiO_2$ electrode and Dye Loading for Dye-sensitized Solar Cells)

  • 황성진;정현상;전재승;김형순
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2010년도 춘계학술대회 초록집
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    • pp.68.2-68.2
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    • 2010
  • Dye-sensitized solar cells (DSSC) show great promise as an inexpensive alternative to conventional p-n junction solar cells. Investigations into the various factors influencing the photovoltaic efficiency have recently been intensified. The conventional absorber electrode in DSSC is composed of compacted or sintered $TiO_2$ nanopowder that carries an anchored organic dye. The absorbance of incident light in the DSC is realized by specifically engineered dye molecules placed on the semiconductor electrode surface ($TiO_2$). The dye absorbs light at wavelengths up to about 920nm, the energy of the exited state of the molecule should be about 1.35eV above the electronic ground state corresponding to the ideal band gap of a single band gap solar cell. The dye molecules ar adhered onto the nanostrutured $TiO_2$ electrode by immersing the sintered electrode into a dye solution, typically 3mM in alcohol, for a long enough period to fully impregnate the electrode. However, the concentrations of the dye is slightly changed due to the evaporation of the alcohol. The dye is more expensive than other materials in DSSC and related to the efficiency of DSSC. Therefore, the concentrations of the dye should be carefully measured. In this study, we investigated to the dye loading on fired $TiO_2$ powder as a function of temperature by the TG-DTA and the dye solution by UV-visible spectroscopy after the impregnation process. The dye loading is related to the porosity of the nanostructured $TiO_2$ electrode.

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반응염료염액에서의 키틴의 염료흡착성능 (Dye Adsorption Ability of Chitin in Reactive Dyebath)

  • 유혜자;김정희;이혜자;이전숙
    • 한국의류학회지
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    • 제26권2호
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    • pp.349-354
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    • 2002
  • In order to decolorize the reactive dye wastewater, we investigated the dye-adsorption ability of chitin, which was natural polymer obtained from shrimp shell. Chitin particle(less than 250 ${\mu}{\textrm}{m}$n) was prepared from shrimp shells in the processes of decalcification in aqueous hydrochloric acid solution and deproteination in aqueous sodium hydroxide solution. The particle size of chitin was controlled to less than 250 ${\mu}{\textrm}{m}$. Three tripes of the reactive dyes-C.I. Reactive Red 120, C.I. Reactive red 241 and C.I. Reactive Black 5-were used. Dye adsorption ability of chitin was investigated by dipping the particle in the dyebaths of concentration of 0.0l%, 0.03% and 0.05% for various periods of time(1,3,5, 10,20,40,80,160minutes). The influence of addition of salt(Na$_2$SO$_4$) and alkali to the dyebaths on dye-absorption was also investigated. We obtained the following results fur the dye-absolution ability of chitin in the dyebaths of three types of reactive dyes. 1) The amount of dye uptake by chitin was increased by addition of salt to the dyebaths. 2) As the concentration of alkali became higher than 3g/I, the amount of dye uptake by chitin was increased. Chitin showed good dye-adsorption ability, when the alkali concentration was high. 3) Chitin showed equal dye uptake in the three types of dyebaths when the dye concentration was 0.0l%. Over 90% of dyestuffs was adsorbed from the dyebaths in ten minutes. When the dye concentration was higher, better adsorption ability was showed in a dye bath of Reactive black 5 than in the others. When the dye concentration was 0.03%, 90% of Reactive red 120 and Reactive red 241 was adsorbed in 40 minutes and the same of Reactive black 5 in 10 minutes. When the dye concentration was 0.05%, 9()% of Reactive red 120 was adsorbed in 80 minutes, and Reactive black 5 in to minutes.

수종의 분산 염료 및 환원염료에 의한 Polyester microfiber의 고견뢰 염색기술 (High Fastness Dyeing Technology of Polyester Microfiber with Several Disperse Dyes and Vat dye)

  • 백진주;권오대;손아름;이난형;김삼수
    • 한국염색가공학회지
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    • 제15권6호
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    • pp.1-7
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    • 2003
  • Polyester microfiber has usually greater dye uptake than normal denier polyester fiber in same dyeing condition. In spite of this high dye uptake dyed microfiber fabric has not only low visual colour depth but also poor washing fastness property. In order to study high colouring dyeing technology and high washing fastness of polyester microfiber, dyeing property of polyester microfiber was investigated according to the dye solubility and particle size of used disperse dyes in aqueous dye solution. After disperse dyeing, dyed fabric with disperse dye was redyed with a vat dye without reduction clearing in order to obtain a high washing fastness property. The result were as followings ; A small particle sized disperse dyes such as C. I. Disperse Blue 56 and Red 60 showed high rate of initial exhaution compared with a large particle sized disperse dyes like C. I. Disperse Blue 165 and Red 343. In study of dyeing property of polyester microfiber with C. I. Vat Blue 1, polyester microfiber could achieve high dye uptake at a given optimum vatting process conditions. On the other hand, in consecutive dyeing with disperse and vat dye, K/S value of polyester microfiber with a small particle sized disperse dye increased without reduction clearings, but K/S value of polyester microfiber with a large particle sized disperse dye decreased with reduction clearings.

Simple Method to Correct Gene-Specific Dye Bias from Partial Dye Swap Information of a DNA Microarray Experiment

  • KIM BYUNG SOO;KANG SOO-JIN;LEE SAET-BYUL;HWANG WON;KIM KUN-SOO
    • Journal of Microbiology and Biotechnology
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    • 제15권6호
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    • pp.1377-1383
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    • 2005
  • In a cDNA microarray experiment using Cy3 and Cy5 as labeling agents, particularly for the direct design, cDNAs from some genes incorporate one dye more efficiently than the other, which is referred to as the gene-specific dye bias. Dye-swaps, in which two dyes are switched on replicate arrays, are commonly used to control the gene-specific dye bias. We developed a simple procedure to extract the gene-specific dye bias information from a partial dye swap experiment. We detected gene-specific dye bias by identifying outliers in an X-Y plane, where the X axis represents the average log-ratio from two sets of dye swap pairs and the Y axis exhibits the average log ratio of four forward labeled arrays. We used this information for detecting differentially expressed genes, of which the additionally detected genes were validated by real-time RT-PCR.

염료·염석결정화계에서 물성과 최적조업조건 (Physical Property and Optimal Operating Condition in the Salting-out Dye Crystallization System)

  • 편유리;한현각;정형기
    • Korean Chemical Engineering Research
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    • 제47권2호
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    • pp.157-162
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    • 2009
  • 본 논문은 소량 다품종 생산체계로 생산되는 정밀화학제품인 고품질 염료제조를 위한 고효율 염료결정화 분리기술에 대한 연구로써 염료 염석결정화계에서 염료의 물성과 최적조업조건에 대해 연구하였다. 먼저 염료의 물리적 특성을 알아보기 위해 밀도, 점도 등을 다양한 조건하에 측정하였다. 염료의 밀도는 물과 비슷한 특성을 보였으며, 염료 용액의 농도가 변화할 때, 밀도 변화는 거의 없었으며, 점도는 농도가 증가 할수록 증가했다. 염료 용액의 온도가 높아짐에 따라 밀도는 증가하였으나, 점도는 감소하였다. 또 염료의 용해도 실험 결과 20 wt% 염료 용액에 15 wt% KCl을 첨가하였을 때 염료결정의 생성량이 최대로 나타났다. 또한, 최적의 조업조건을 찾기 위해 다양한 변수(온도, RPM, 염첨가 방법 등)를 제어하여 시간에 따라 실험하였다. 15 wt% KCl과 20 wt% 염료용액으로 실험한 결과 최적의 조업조건은 $25^{\circ}C$, 100 RPM, 염을 한 번에 넣는 방법으로 나타났다. 또 최적 조업조건으로 실험을 수행한 결과 기존의 공정($35^{\circ}C$, 150 RPM)보다 생성량이 증가하였다. 따라서 최적화 공정을 찾음으로써 생성량 증가와 에너지 저감 효과가 기대된다.

색소의 혼합법과 분리법에 의한 색소레이저 에너지 증가의 비교 (Enhancement of Dye Laser Energy by Mixed and Separate Method)

  • 서욱창
    • 한국광학회:학술대회논문집
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    • 한국광학회 1989년도 제4회 파동 및 레이저 학술발표회 4th Conference on Waves and lasers 논문집 - 한국광학회
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    • pp.46-48
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    • 1989
  • In order to increase the efficiency of a bluegreen dye laser, the mixed dye method and separate dye method have been employed and the results were compared. By the mixed dye method(LD490+BBQ), the enhancement of laser energy was 193%, while by the separate dye method(LD490/BBQ), it was 90%, It was also found that the mixed dye method made the laser threshold energy decreased.

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