• 한국양식학회지
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• 제2권1호
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• pp.43-51
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• 1989

녹조 편모류인 Dunaliella tertiolecta Butter를 광도, 광주기 및 온도 조건을 달리하여 배양한 후 조체내의 지방산 조성을 분석하였다. 광주기와 온도 조건은 D. tertiolecta의 성장에 뚜렷한 영향을 주었고, 성장기에 최대 세포수는 $2.32{\times}10^6$, 세포 분열율은 1.97 division/day였다. 여러조건에서 배양한 조체의 지방산을 분석한 결과Cl6(Palmitic acid), C18 : 3$\omega$3 (cis-Linolenic acid)가 주성분이었으며 EPA나 DHA와 같은 고도의 불포화 지방산은 소량 검출되었다. 불포화 지방산(Polyene)은 배양 온도가 낮을수록 증가되었으며, 광도는 불포화 지방산 함량에 별로 영향을 미치지 못했다. 광주기 조건에서, 명주기를 오래할수록 D. tertiolecta의 성장은 증가했지만, 반면에 조체내의 불포화 지방산(Polyene)은 감소되는 것으로 나타났다.

• 한국양식학회지
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• 제17권2호
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• pp.151-157
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• 2004

Effects of supplementing selected fatty acids on fatty acid incorporation (17 days) , and progeny production (14 days) in Artemia franciscana (Great Salt Lake, USA) were studied. To compare with the control four diets, which differed in fatty acid composition alone contain Dunalieia tertiolecta and an emulsion either rich in OA (oleic acid, 18: 1 n-9), ARA (arachidonic acid, 20:4n-6), EPA (eicosapentaenoic acid,20:5n-3), or DHA (docosahexaenoic acid, 22:6n-3). Each of these emulsions was supplemented at a ratio of 20 % of the daily dose of D. tertiolecta (% algal dry weight). The initial OA and ARA values were 33.5 and 1.7 mg/g DW of freshly-hatched nauplii, respectively. After 11 days of feeding, these values increased to 38.8 and 7.6 mg/g DW in Artemia receiving the fatty acid sup-plement rich in each of the respective fatty acids. After 14 days, the levels were almost doubled, reaching 62.8 and 13.4 mg/g respectively. On EPA supplementation, its level after 11 days of feeding was 14.3 and 17.3 mg/g in male and female, respectively and was 16.0 and 23.1 mg/g in the male and female after 14 days, respectively. The EPA accumulated more in the body (39.1 mg/g) than in ovisac (16.9 mg/g). In the DHA supplementation group also, DHA levels after 11 days of feeding were 3.1 and 5.5 mg/g in male and female, respectively. After 14 days, the DHA level continued to increase in male. but slightly decreased to 4.6 mg/g in female. It was not richer in ovisac (2.6 mg/g) than in the remaining body of female (4.6 mg/g). In conclusion, fatty acids supplied by a lipid emulsion as a supplement to the algal diet are well incorporated in the adult Artemia. Apart from being an extra source of energy, these emulsions may function as source of HUFA which may play an essential role for growth and progeny production (fecundity) of Artemia.