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Surface ultrastructures of the third-stage larvae of Gnathostoma hispidum (돼지악구충(Gnuhostoma hispidum) 제3기 유충의 표면 미세구조)

  • 손운목;이진하
    • Parasites, Hosts and Diseases
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    • v.34 no.3
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    • pp.169-176
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    • 1996
  • A scanning electron microscopic study was performed to observe the surface ultrastructures of the third-stage larvae of Gnathostoma hispidun. The early third-stage larvae (EL3) were collected from the viscera of Chinese loaches by the artificial digestion method . The advanced third-stage larvae (AdL3) were recovered from mice experimentally infected with EL3. Both larval worms were fixed with 2.5% glutaraldehyde, dehydrated in graded alcohol. dryad in critical point dryer, and coated with gold. The specimens were observed with a SEM (DS- l30C). On the head bulb of both larval stage, the mouth had a pair of lateral lips of equal size and of half moon shape. Each lip had a couple of labial papillae and a small amphid located between the two papillae. The hooklets on the head bulb had single-pointed tips and curved posteriorly. The cuticular spines of EL3 were larger and more densely distributed in the anterior area (about 1.8 Mm in length) and gradually decreased in size and number posteriorly. The cuticular spines in the anterior area of AdL3 were sharp-pointed and about 4.5 Mm in length, and those in the middle area were about 1.75 Mm. The velvety cuticular folds and dot-like cuticular spines were distributed in the posterior area. A cervical papilla was located between the 7th and 8th transverse striations. A dome-like body papilla was located at the posterior 1/4 of body. An ellipsoidal excretory pore was located between the 17th and 18th striations. From the above results, it is suggested that the characteristic SEM findings obtained from this study may be helpful on the species identification of larval Gncthostomn.

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Growth and Yield Characteristics of Foxtail Millet, Proso Millet and Sorghum According to Sowing Date in Middle Area in Korea (중부지방에서 파종기에 따른 조, 기장, 수수의 생육 및 수량 특성)

  • Yoon, Seong-Tak;Jae, Eun-Kyeong;Kim, Young-Jung;Jeong, In-Ho;Han, Tae-Kyu;Kim, Tae-Yun;Cho, Young-Son;Kang, Hang-Won
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.2
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    • pp.197-211
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    • 2015
  • In order to investigate optimum and marginal sowing date of foxtail millet, proso millet and sorghum in middle area (Anseong, Gyeonggi province) of korea, four levels of sowing date [(May 15(1st), June 6(2th), June 26(3th), July 13(4th)] with mulching and non-mulching were treated in this experiment. We used three crops of foxtail millet, proso millet and sorghum with four varieties of Samdachal, Samdamae, Kyeongkwan1, Hwanggeumjo in foxtail millet, Leebaekchal, Manhongchal, Hwangsilchal, Hwanggeumgijang in proso millet and Nampungchal, Hwanggeumchal, DS202, Moktaksusu in sorghum achieved from RDA. In culm length, sowing date of June 26 was the highest in all varieties of foxtail millet, proso millet and sorghum. Sowing date of May 15 showed the highest ear length in foxtail and proso millet, whereas the highest ear length was obtained from sowing date of June 26 in sorghum. In numbers of leaf, sowing date of May 15 showed the highest in all three crops of foxtail millet, proso millet and sorghum. Days from sowing to heading date were reduced in Samdamae, Kyeongkwan1 and Samdachal as sowing date was late. In Hwanggeumjo it was reduced from the sowing date from May 15 to June 26, but it was same days was taken between June 26 and July 13 sowing date. Days from sowing to heading date of four varieties in proso millet were reduced as sowing date was late. Days from sowing to heading date in sorghum were reduced as sowing date was late, whereas it was increased the $4^{th}$ sowing date of July 13. There were no significant differences in growth and yield characteristics between mulching and non-mulching in four varieties of foxtail millet, proso millet and sorghum with four varieties. The highest grain numbers per panicle, 1000 grain weight and yield per 10a were obtained from sowing date of June 26 in four varieties of foxtail millet, proso millet and sorghum. In sowing date of July 13, all varieties of foxtail millet, proso millet and sorghum were not able to be ripened because of late sowing except Hwnaggeumjo, Manhongchal and Hwanggeumgijang.

Effect of Soil Salinity on Growth, Yield and Nutrients Uptake of Whole Crop Barley in Newly Reclaimed Land (신간척지에서 토양 염농도가 청보리 생육, 수량 및 양분 흡수에 미치는 영향)

  • Lee, Sang-Bok;Cho, Kwang-Min;Shin, Pyung;Yang, Chang-Hyu;Back, Nam-Hyun;Lee, Kyeong-Bo;Baek, Seung-Hwa;Chung, Doug-Young
    • Korean Journal of Environmental Agriculture
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    • v.32 no.4
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    • pp.332-337
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    • 2013
  • BACKGROUND: Newly reclaimed land has poor soil environment for crop growth since it is high in salt concentration but low in organic content compared with ordinary soil. It is known that whole-crop-barley can grow better in the soil of relatively high salt concentration than other crops but, the growth is poor at the concentration if higher than certain amount and it is a difficulty to secure productivity. Hence, the level of soil salt concentration suitable for the production of bulky feed in newly reclaimed land has been investigated. METHODS AND RESULTS: At Saemanguem reclaimed land, the land for the soil salt concentration electrical conductivity (EC) 0.8, 3.1, 6.5, 11.0 dS/m was selected; and chemical fertilizer $N-P_2O_5-K_2O$ (150-100-100kg/ha) was tested; and forage barley 220kg/ha were sown. The soil salt concentration during the cultivation period decreased in the order of harvest season>earing season>sowing season>wintering season, and the salt concentration in harvest season is 1.4-4.2 times higher than that of the sowing season. The higher the salt concentration, the poorer the over ground growth due to poor rooting; especially at EC 11.0 ds/m there was emergence but, it blighted after wintering. The Yield from the soil salt concentration 3.1dS/m and 6.5 dS/m was 68% and 35% from that of the soil salt concentration 0.8 dS/m (8.8 MT/ha) respectively. The proline content in early life stage was more than that of the harvest season, and it increased with salt concentration. The higher salt concentration, the more $Na_2O$ and MgO content in harvest season; but the higher the salt concentration, the less the content of N, $P_2O_5$, $K_2O$ and CaO. CONCLUSION(S): When the soil salt concentration becomes higher than 3.1 dS/m, the yield becomes poor because there is serious growth inhibition of forage barley both in root part and above aerial part that results in unbalanced absorption of nutrients. Therefore, it is recommended that the salt concentration should be lowered below 3.1 dS/m by underground drainage facilities or irrigating water for the stable production of whole-crop-barley.

A Study on the Surgical Hand Scrub and Surgical Glove Perforation (외과적 손씻기 및 외과용 장갑의 천공율에 대한 연구)

  • 윤혜상
    • Journal of Korean Academy of Nursing
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    • v.25 no.4
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    • pp.653-667
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    • 1995
  • Post - operative wound infections have been a serious problem in nursing care in the operating room and appear to be strongly related to the infection occurring during the performance of operation. The purpose of this study is to identify patterns in duration of surgical hand scrub (SHS), to evaluate the method of SHS and to examine the rate of glove perforation. Subjects for this study include 244 doctors and 169 nurses working in the operative theatre of a hospital in Seoul area. Test samples and related data were collected from this medical facility between April 1, through 15, and July 1, through 5, 1995 by the author and a staff member working in the operating room. For the study, data on the SHS of doctors and nurses were obtained at the time of operation and multiple batches of surgical gloves worn by the operating doctors were collected after each operation. The duration of SHS was measured with a stop watch and the method of SHS was evaluated according to Scoring Hand Scrub Criteria (SHS Criteria) and expressed as SHS scores. For the analysis of the data, t-test was used to compare the differences in the duration and the SHS scores of doctors and nurses, and Pearson's correlation coefficient was used to examine the relationship between the SHS duration and the SHS scores. The results of the study are summarized as follows. 1) The mean time spent in each SHS was 167 seconds in nurses, and 127 seconds in doctors. The data comparing nurses and doctors indicated that there were significant differences in Our ation of SH S between these two groups (t=5.58, p=.000). 2) The mean time spent in the first SHS was 145 seconds and that in the End SHS, 135 seconds, and there was not a significant difference in the duration of the SHS between doctors and nurses (t=1.44, P=.156). 3) The mean time spent in the SHS by OS (Orthopaedic surgery) doctors was 162 seconds, 150 seconds by NS(Neurologic surgery), 121 seconds by GS(General surgery), 94 seconds by OPH(Opthalmology) and DS(Dental surgery), 82 seconds by URO(Urology), 78 seconds by PS(Plastic surgery) and 40 seconds by ENT(Ear, Nose & Throat) These also showed a significant difference in the duration of the SHS among the medical specialities (t=4.8, P=.0001). 4) The average SHS score of the nurses was 15.2, while that of doctors was 13.1. The statistical analysis showed that t-value was 3.66, p was. 000. This indicates that the nurses actually clean their hands more thoroughly than the doctors do. 5) The average SHS score of NS doctors was 15.5, 15.3 for doctors for OPH,14.3 for OS,12.7 for GS, 12.0 for DS, 11.7 for URO, 10.1 for PS, 7.5 for ENT. Comparison of the average SHS scores from 8 specialties showed that there was a significant differences in the patterns of the SHS (F=5.08, P=.000) among medical specialties. 6) It appears that the operating personnel scrub the palms and dorsum of their hand relatively well, however, less thorough the nails and fingers. 7) The more the operating personnel spend their time in hand scrubbing, the more correctly they clean their hands(r=.6427, P<.001). 8) The overall frequencies of perforation in all post-operative gloves tested was 38 out of 389 gloves (10.3%). The perforation rate for PS was 13%, 12.1% for GS,8.8% for 05, and 3.3% for NS.

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Inhibitory Effects of Ginger and Beopje Ginger on DSS-induced Colitis in Mice (생강과 법제생강의 DSS(Dextran Sulfate Sodium)로 유도된 마우스의 대장염 억제 효과)

  • Kim, Sin-Jeong;Kim, So-Hee;Lim, Yaung-Iee;Kim, Yong-Gyu;Park, Kun-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.4
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    • pp.477-484
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    • 2014
  • The purpose of this study was to examine the anti-inflammatory effects of ginger and processed (Beopje) ginger on colitis induced by 2.5% dextran sulfate sodium (DSS) in Balb/c mice. Beopje means a process that herbal medicines are treated by a specific Korean traditional method in order to obtain better pharmacological effects. Mice were fed saline or two different doses of ethanol extracts (ginger and processed (Beopje) ginger) once a day for 14 days. Colitis was induced from day 7 to 14 via administration of 2.5% DSS in drinking water. Experimental animals were divided into four groups: Nor (Normal, 200 ${\mu}L$ of saline without 2.5% DSS-treated group), Con (Control, 200 ${\mu}L$ of saline and 2.5% DSS treated group), G (500 mg/kg of ginger and 2.5% DSS treated group), and BG (500 mg/kg of Beopje ginger and 2.5% DSS treated group). Body weights of both ginger-administered groups increased compared to the control. Colon length increased to 7.6, and 8.0 cm in the G and BG groups, respectively, whereas that of control was 5.7 cm. Histological colon injury induced by DSS-induced colitis was reduced (P<0.05). In serum and DSS-treated colon tissues, mRNA expression levels of IFN-${\gamma}$, IL-6, TNF-${\alpha}$, and IL-12 of the Beopje ginger-treated group were significantly suppressed compared to those of the ginger-treated groups. Expression levels of iNOS and COX-2 of the Beopje ginger-treated group were significantly reduced compared to those of the ginger-treated groups (P<0.05), and BG showed stronger anti-inflammatory effects on colitis. These results indicated that ginger exerted anti-inflammatory effects on DSS-induced colitis in mice, and its effects could be increased through Beopje.

Distribution of Disease Severity and Group A Rotavirus Genotypes (VP7 & VP4) in Children with Acute Rotavirus Gastroenteritis (급성 로타바이러스 장염 소아에서 질환의 중증도와 로타바이러스 VP7 & VP4 유전형의 분포에 대한 연구)

  • Oh, Hyun-Ju;Kang, Hyun-Sik;Kang, Ki-Soo;Kim, Youn-Woo;Hong, Jung-Yun;Shin, Kyung-Sue;Lee, Jin-Sook;Lee, Su-Hyun;Lee, Keun-Hwa;Cho, Moon-Je;Suh, Dong-In
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.14 no.2
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    • pp.148-154
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    • 2011
  • Purpose: We aimed to study the distribution of rotavirus genotypes (VP7 and VP4) and disease severity of rotavirus gastroenteritis prevalent in our community. Methods: Stool samples were collected from 156 children who were hospitalized with rotavirus gastroenteritis from December 2007 to June 2008. The disease severity of all patients was scored using the Vesikari scale. After extraction of ds-RNA of the rotavirus, cDNA synthesis using reverse transcription and polymerase chain reaction (RT-PCR) and multiplex PCR was performed. Following this, the final identification of genotypes was performed. Results: Of the 156 samples, VP7(G) and VP4(P) genotypes were identified in 147 (94.2%) and 140 (89.7%) samples, respectively. G1 (116 of 147 samples; 78.9%) and P[8] (137 of 140 samples; 97.9%) were the most prevalent, respectively. Of the 138 samples identified of combination types of VP7 and VP4, G1P[8] (111 samples; 80.4%) was the most prevalent. Other combination types varied with very low distribution rates. 9.4% of genotypes were not included in the new vaccines. The disease severity score was $11.8{\pm}3.3$ ($mean{\pm}2SD$). The distribution of disease severity was mild or moderate in 37.8% and severe in 62.2% of patients. Conclusion: The most prevalent genotype combination of rotavirus was G1P[8] and genotypes not included in the vaccines represented 9.4% in our community. Disease severity distribution of hospitalized children with rotavirus gastroenteritis was higher in the severe than in the mild and moderate categories.

Effect of Feeding Multiple Probiotics on Performance and Fecal Noxious Gas Emission in Broiler Chicks (혼합 생균제의 급여가 육계의 생산성 및 계분의 유해가스 발생에 미치는 영향)

  • Yoon C.;Na C. S.;Park J. H.;Han S. K.;Nam Y. M.;Kwon J. T.
    • Korean Journal of Poultry Science
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    • v.31 no.4
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    • pp.229-235
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    • 2004
  • A study was conducted to examine the effect of dietary supplementation of multiple probiotics (EM) on growth performance, blood cholesterol, intestinal micro flora, and fecal gas emission in broiler chicks. A total of 450 one day old male broiler chicks (Ross $\times$ Ross) were divided into six treatments with five replications in each treatment for five weeks. Treatments were factorially designed with two levels of diet containing probiotics (DW; 0, $0.2\%$) and three levels of drinking water containing probiotics (DW; 0, 0.01, $0.1\%$). Basal diets contained $21.5\%$ CP and 3,100 kcal/kg ME for starting and $19\%$ CP and 3,100 kcal/kg ME for finishing period. Weight gain, feed intake, and feed conversions of birds fed with probiotics were not significantly different between Ds. Total cholesterol and triglyceride levels were significantly lower (P<0.05) in birds fed with DW $0.01\%$ or $0.1\%$ compared with no probiotics group, but there was no significant difference between D treatments. The number of E. coli, Salmonella and Lactobacillus in the ileum and cecum of the birds fed multiple probiotics were not significantly different from those of no probiotic groups. There were no significant differences in the $CO_2$ gas emissions of fecal between birds fed with Ds or among birds fed with DW. However, $NH_3$ gas emissions of DW $0.1\%$ were significantly lower (P<0.05) than DW $0\%$. In the results of this study, supplementation of probiotics tended to decrease the serum cholesterol and triglyceride compared to those of control groups and reduction of fecal $NH_3$ gas emission.

A study for Beating Filter Press Dewatering Technology (열(熱) 필터프레스 기술(技術)을 통한 슬러지 탈수율(脫水率) 향상(向上)을 위한 연구(硏究))

  • Lee, Jung-Eun;Kim, Dong-Su
    • Resources Recycling
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    • v.15 no.3 s.71
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    • pp.38-45
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    • 2006
  • The thermal filter press dewatering(TFPD) technology to improve the dewaterability through increasing the inner vapor pressure, lowering the filtration viscosity and forming the porosity easily within cake as applying the heat at the sludge layer was developed in this study. The hot water with temperature of $95^{\circ}C$ and pressure of $1.2kg_f/cm^2$ was supplied to the heating plate equipped between filter plates with plate size of $470{\times}470mm$ and material of polypropylene. Sludge was dewaterd by supplying pressure of $5kg_f/cm^2$ and then by squeezing pressure of $15kg_f/cm^2$. As a results of estimating the characteristics of thermal dewatering to consider the initial water content and organic content to be influenced by a period of water shortage and rainwater, the dewatered cake water content was about 35 wt% and dewatering velocity was $4DSkg/m^2{\cdot}hr$ under the rainwater period, and the dewatered cake water content was about 50 wt% and dewatering velocity was $1.5DSkg/m^2{\cdot}hr$ in the case of sludge of water shortage season. These results was superior to the mechanical dewatering performance with water content of 70wt% and dewatering velocity of $0.9DSkg/m^2{\cdot}hr$. On the base of the results of TFPD, energy consumpted to deal with DS(Dry Solid) of 1kg was estimated by 300 kJ. It was analyzed that the energy consumption of TFPD was decreased about one third with comparison to the dryer system. Dewatering velocity of this technology was faster than the one of mechanical dewatering equipment and it was easier to product low water content cake. Therefore, this technology was recognized that dewaterability was predominant because of the fast of dewatering velocity and production of low water content cake, and also this known as economical efficiency was excellent because of low energy consumption in comparison with dryer.

Protective Effect of Kefir Grain Against Dextran Sodium Sulfate-Induced Colitis in Rats (Dextran Sodium Sulfate로 대장염을 유도한 흰쥐에서 캐피어 원말의 장보호 효과)

  • Ko, Young-Eun;Kim, Mi-Kyoung;Cho, Han-Young;Lee, In-Young;Ly, Sun-Yung
    • Journal of Nutrition and Health
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    • v.41 no.5
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    • pp.391-401
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    • 2008
  • Probiotics have emerged as a potential treatment modality for numerous gastrointestinal disorders, including IBD. However, few probiotics have undergone appropriate preclinical screening in vivo. Kefir is considered a probiotic, benefiting the host through its effects in the intestinal tract. Despite numerous studies examining the action of probiotics on the host organism, few have analyzed the effects on intestinal environment. We assessed the protective effect of kefir for three weeks before inducing colitis with 2% dextran sodium sulfate for five days. The DSS loads were similar in all DSS treatment group. The results of the experiment are as follows. Food intake and FER of experimental groups were not significantly different each other, but water consumption tended to be higher in all DSS treatment groups as compared with the normal control. And visual inspection of feces revealed mild diarrhea in rat given 2% DSS. The anti-inflammatory activity of kefir was determined by myeloperoxidase activity during the DSS treatment, and there was no significant difference in any group. The levels of thiobarbituric acid reactive substances (TBARS) as a colonic lipid peroxidation were significantly lower in the kefir intake groups than in rats treated with 2% DSS alone. The DNA % in tail and tail moment values as a DNA damage level of the blood lymphocytes in kefir intake groups tended to be lower than 2% DSS treatment alone, especially tail lengths were significantly diminished. According to the colonic histopathological assay, there were a severe inflammation of lamina propria and submucosa and mild edema in mucosa and sub mucosa in DSS alone treated group. We found a slight regenerative change in kefir treatment groups. In our experiments, this means that ulcerative colitis related to oxidative injury might be prevented by kefir as a probiotic. Further studies of the potential benefits of kefir as a probiotic in inflammatory condition are encouraged.

The Association of Oral Impacts on Daily Performances for Children (C-OIDP), Oral Health Condition and Oral Health-Related Behaviors (어린이 일상생활구강영향지수(C-OIDP)와 구강관리 및 구강건강행태와의 관련성)

  • Jo, Hwa-Young;Jung, Yun-Sook;Park, Dong-Ok;Lee, Young-Eun;Choi, Youn-Hee;Song, Keun-Bae
    • Journal of dental hygiene science
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    • v.16 no.3
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    • pp.242-248
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    • 2016
  • The purposes of this study were to investigate the factors affection the Oral Impacts on Daily Performances for Children (C-OIDP) in elementary and middle school students, and identify the association between oral health-related behaviors, oral health condition and C-OIDP. A cross-sectional study was conducted in three schools in Incheon, Asan, Korea. A total of 175 selected children were interviewed by a trained examiner using a questionnaire. Oral Health Related Quality of Life was assessed by the Korean version of C-OIDP. Socio-economic characteristics, oral health-related behaviors, oral health condition and C-OIDP were verified using the questionnaire. ANOVA analysis was performed to determine the oral health and C-OIDP, and multiple regression analysis was performed to determine the factors affecting the C-OIDP. The activities with the greatest effect were eating (28.0%), cleaning teeth (22.9%), and smiling (18.9%). In the logistic regression model, the high item score of C-OIDP was associated with experiencing dental caries and gum pain in the past month. The more the C-OIDP prevalence item, the more the fillng deciduous tooth surface (fs) (p=0.024), caries experienced deciduous tooth surface (dfs) (p=0.049), total caries tooth surface (ds+DS) (p=0.021), and total caries experienced tooth surface (dfs+DMFS) (p=0.047). It can be concluded that the factors affecting C-OIDP are fs, dfs, dfs+DMFS, and gingival pain. Based on these results, we can improve C-OIDP to advance preventive practice.