• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.10a
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• pp.657-659
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• 2010

Rhizoma of Dryopteris crassirhizoma, Root of Glycyrrhiza uralensis, Root of Sophora flavescens and Pericarp of Citrus unshiu were extracted with ethanol. The ethanol extracts of 4 medicinal plant were tested for the antimicrobial activity against Streptococcus mutans. The extracts of Rhizoma of Dryopteris crassirhizoma, Root of Sophora flavescens, Root of Glycyrrhiza uralensis showed antimicrobial activity against Streptococcus mutans. At the 300ug/disk concentration of the ethanol extract from Dryopteris crassirhizoma and Sophora flavescens showed significant antimicrobial activity against Streptcoccus mutans. These results suggested that the extracts from Dryopteris crassirhizoma could be the potential source of antimicrobial agents against S. mutans.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2011.10a
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• pp.934-937
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• 2011

As part of our screening of anticariogenic agents from medicinal plants, the ethanol extracts of 10 herbs widely used in the fork medicine were tested for the antimicrobial activity against a cariogenic bacterium Streptococcus sobrinus. The ethanol extracts of Dryopteris crassirhizoma showed antimicrobial activities against S. sobrinus. These results suggested that the extracts of Dryopteris crassirhizoma could be the potential source of antimicrobial agent against S. sobrinus.

• Biomedical Science Letters
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• v.13 no.3
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• pp.189-195
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• 2007

This study was carried out to examine antimicrobial substances from medicinal plants, the ethanol extracts of 38 medicinal plants were tested for the antimicrobial activity against Staphylococcus aureus ATCC 25923 and methicillin-resistant Staphylococcus aureus ATCC 43300. The extracts of Glycyrrhiza uralensis, Sophora flavescens, Dryopteris crassirhizoma, and Pinas densiflora showed significant antimicrobial activities against both S. aureus ATCC 25923 and methicillin-resistant S. aureus ATCC 43300. The extract of Dryopteris crassirhizoma among these medical plants showed the highest antimicrobial activity. These results suggested that the extracts from Dryopteris crassirhizoma, Sophora flavescens, Pinas densiflora, and Glycyrrhiza uralensis could be the potential source of antimicrobial agents against methicillin-resistant S. aureus and S. aureus.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.1087-1090
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• 2009

This study was carried out to research antimicrobial agents from medicinal plants, Glycyrrhiza uralensis, Dryopteris crassirhizoma, Dictamnus albus, Paeonia lactiflora, Angelica dahurica, Spirodela polyrhiza, Cimicifuga heracleifolia, Bupleurum falcatum, Magnolia kobus, Artemisia princeps, Arctium lappa, Aster tataricus, Hovenia dulcis, Citrus unshiu, Asparagus cochinchinensis, Gardenia jasminoides, Smilax china, Hovenia dulcis, Prunus sargentii, Scutellaria baicalensis. The ethanol extracts of 20 medicinal plants were tested for the antimicrobial activity against Agrobacterium tumefaciens. The extracts of Glycyrrhiza uralensis, Dryopteris crassirhizoma, Cimicifuga heracleifolia, Bupleurum falcatum showed antimicrobial activities against Agrobacterium tumefaciens.

• Korean Journal of Veterinary Research
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• v.48 no.4
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• pp.473-480
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• 2008

A high incidence of Anisakiasis has been reported in many countries where people eat frequently raw or undercooked seafood. Anisakis spp. larvae were obtained from the mackerel acquired from a fish market of Cheongju city. They were divided into several groups and placed in culture dishes containing RPMI-1640 (culture media), in the presence or absence of different concentrations of herbal extracts (Meliae ezadarach, Dryopteris crassirhizoma, Quisqualis indica var villosa). The objective of the present study was to investigate the activity of larval migration inhibition in vitro. Meliae ezadarach at the concentrations of 7.5, 15, and 30 mg/ml effectively inhibited the larvae migration in time-dependent manner during experimental period of 0-24 h. Treatment of Meliae ezadarach at the three concentrations completely inhibited the larvae migration in vitro. Dryopteris crassirhizoma at the concentrations of 5, 10, and 20 mg/ml also effectively inhibited the larvae migration in a time-dependent manner. The treatment of Dryopteris crassirhizoma for 12 h completely inhibited the larvae migration. The inhibitory effect of Dryopteris crassirhizoma was stronger than that of Meliae ezadarach. Although Quisqualis indica var villosa also showed the inhibitory effect on larvae migration, its inhibitory efficacy was the weakest among tested herbal extracts. These results indicated that some herbal extracts may be useful in controlling human anisakiasis.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.3 s.58
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• pp.201-208
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• 2006

Propionibacterium acnes have been recognized as pus-forming bacteria triggering an inflammation in acne. The present study was conducted to evaluate antimicrobial activities of Dryopteris crassirhizoma Nakai against these etiologic agents of acne vulgaris and application possibility as a cosmetic resource. D. crassirhizoma crude extract and hexane fraction was prepared and its anti-acne effect against Propionibacterium acnes was investigated with minimum inhibitory concentration (MIC) and paper disk diffusion method. The MIC of D. crassirhizoma crude extract and hexane fraction was 0.008 mg/mL and 0.001 mg/mL, respectively. This implies that D. crassirhizoma extract may be an efficient anti-acne ingredient for cosmetics, as a crude extract. The paper disk diffusion assay showed that its anti-acne effect was similar to that of triclosan. The cytotoxic effect of D. crassirhizoma extract was determined by a colorimetric MTT assay using HaCaT cell line and D. crassirhizoma extract exhibited lower cytotoxic effects. Finally, we examine the stability of D. crassirhizoma extract to temperature and pH. The D. crassirhizoma extract was very stable to high temperatures ($25{\sim}121^{\circ}C$) and to wide pH range ($pH\; 2{\sim}11$), suggesting its utilization for cosmetics.

• Journal of Korean society of Dental Hygiene
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• v.5 no.1
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• pp.123-130
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• 2005

This study was executed to evaluate the effects of natural herbal medicine extract on the antimicrobial activity and the artificial plaque formation. Schisandra chinensis, Sophora flavescens, Dryopteris crassirhizoma and Aristolochia contorta were extracted by 80% MeOH respectively and each concentration(5mg, 10mg, 20mg) of herbal medicine extract was tested to show the antimicrobial activity against S. mutans. To show the inhibition effect of herbal medicine extract on the artificial dental plaque formation each extract of 0.4% concentration was added into M 17 broth and inoculated with $5.0{\times}10^6$ of S. mutans. After 24 hour incubation each weight of artificial dental plaque was measured and compared with each other. Results obtained are as follows: 1. Dryopteris crassirhizoma showed very intense antimicrobial effect but Schisandra chinensis, Sophora flavescens and Aristolochia contorta showed a little difference against control. 2. Regarding artificial plaque formation inhibiting activity by each herbal extract at 0.4% concentration, Dryopteris crassirhizoma showed the perfect inhibition effect. Schisandra chinensis and Aristolochia contorta followed in order but Sophora flavescens showed the worst value. In conclusion, Dryopteris crassirhizoma showed strong antimicrobial effect against S. mutans and significant inhibiting effect on the artificial dental plaque formation.

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.611-618
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• 2001

The ethanol extract of Dryopteris crassirhizoma Nakai showed strong growth inhibition against 5 strains of Listeria monocytogenes at the concentrations of $100{\sim}500$ ppm and the minimum inhibitory concentration of n-hexane fraction was under 50 ppm. The D8-2-5 fraction isolated from n-hexane fraction of Dryopteris crassirhizoma Nakai showed a strong bactericidal activity on 5 strains of L. monocytogenes at 20 ppm level in tryptic soy broth medium. At the level, the viable count was reduced $4{\sim}6$ log cycle compared to initial cell number. Observation by the measurement of adenosine triphosphate (ATP) contents and transmission electron microscope showed that disruptions of the cell wall and elution of intracellular ATP are assumed to be due to the bactericidal activity. In addition, the n-hexane fraction of Dryopteris crassirhizoma Nakai showed the strong growth inhibitions at 50 ppm on Vibrio parahaemolyticus and Bacillus cereus, and at 25 ppm on Staphylococcus aureus.

• Journal of the Korean Society of Clothing and Textiles
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• v.25 no.1
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• pp.3-12
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• 2001

The dyeing powder drawn out from Dryopteris crassirhizoma by water was concentrated. Using this powder, the silk and the cotton fabrics were dyed and they measured with the K/S value, surface color, mordant quantity in order to evaluate the dyeability, antimicrobial and deodorant properties. The colorant of Dryopteris crassirhizoma was proved flavonoids by FT-IR spectrum. The K/S values of silk were much higher than those of cotton, the color yield of the silk and cotton fabric were most efficient the postmordanting method. The surface colors on the dyed fabric depended heavily upon mordants used or mordanting methods. For all cases, the value of the dyed fabric was generally dark. The chroma produced clear for the Al-mordant of silk and the Cu-mordant of cotton in the 3% concentration of mordants, the color difference was distinct when using the Fe-mordant. The K/S values of cationized cotton were much higher than cotton. The color fastness was significantly improved when mordants were added, In the case of the light fastness, Fe and Cu-mordants improved more than 1 level. The Cu-mordant showed the greatest antimicrobial and deordarant activity on both of the silk and the cotton.

• Korean Journal of Plant Resources
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• v.25 no.2
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• pp.176-183
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• 2012

This study was carried out to develop ferns as the natural antioxidant materials by graduating and extracting fronds of $Dryopteris$ $crassirhizoma$, $Dryopteris$ $nipponensis$, and $Polystichum$ $lepidocaulon$, which belong to Dryopteridaceae, using solvent, and analyzing the antioxidant effect of each fraction. The n-butanol fraction of $D.$ $crassirhizoma$ (550.0 $mg{\cdot}g^{-1}$), the ethyl acetate fraction of $D.$ $nipponensis$ (374.8 $mg{\cdot}g^{-1}$), and the n-butanol fraction of $P.$ $lepidocaulon$ (781.8 $mg{\cdot}g^{-1}$) showed relatively higher total contents of polyphenol. The chloroform fraction of $D.$ $crassirhizoma$ (72.9 $mg{\cdot}g^{-1}$), and the n-hexane fraction of $D.$ $nipponensis$ (72.9 $mg{\cdot}g^{-1}$) and $P.$ $lepidocaulon$ (154.5 $mg{\cdot}g^{-1}$) contained relatively higher total contents of flavonoids. DPPH radical scavenging activity was most excellent in the n-butanol fraction of $D.$ $crassirhizoma$ ($RC_{50}=0.02mg{\cdot}mL^{-1}$) and $P.$ $lepidocaulon$ ($RC_{50}=0.04mg{\cdot}mL^{-1}$), and the water fractions of $D.$ $nipponensis$ ($RC_{50}=0.01mg{\cdot}mL^{-1}$). ABTS radical scavenging activity was potent in the n-hexane and n-butanol fractions of $D.$ $crassirhizoma$ (each $RC_{50}=0.02mg{\cdot}mL^{-1}$), the ethyl acetate fraction of $D.$ $nipponensis$ ($RC_{50}=0.03mg{\cdot}mL^{-1}$), and the n-butanol fraction of $P.$ $lepidocaulon$ ($RC_{50}=0.06mg{\cdot}mL^{-1}$). There was the large amount of total polyphenol content in the n-butanol fraction of $D.$ $crassirhizoma$ and $P.$ $lepidocaulon$, and their radical scavenging activities were potent. Therefore, it was thought that biologically active substances of each fraction layer are required to be analyzed and used.