• Korean Journal of Medicinal Crop Science
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• v.15 no.4
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• pp.251-255
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• 2007

Floret flowering of a monocarpic plant Orostachys japonicus should be controlled to change its harvest time. The study was done to clarify the effect of pinching time of inflorescence with many florets (Aug. 25, Sept. 8 and 22, Oct. 4) on its growth, morphological characters, and flowering. Night-break treatment of 2 hours at midnight were done since Aug. 25 when afterward samples were taken every 2 weeks to Nov. 4. Growth, morphological and flowering related characters were measured from each sample. Plant height, inflorescence length and number of leaves including bracts showed significant difference between the treatments because they were sharply increased until each pinching time. Stem diameter did nearly same. Leaf and bract, floret, shoot and total dry weights had similar response to the plant height, but stem and root dry ones did similar response to the stem diameter. Most florets were removed by the pinching treatments after formed ones were increased until each pinching time. Few florets were left after the treatment, meaning that night-break treatment after that could be skipped.

• Korean Journal of Medicinal Crop Science
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• v.16 no.1
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• pp.16-19
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• 2008

Shoot parts of a monocarpic and short day plant Orostachys japonicus have been used as a herbal medicine. The study was carried out to examine the effect of transplanting time (May 30, June 30, July 30) of its seedlings on its growth, morphological characters, and flowering. Two hours night-break treatment at midnight was done since Aug. 25 when samples were taken afterward every 4 weeks. Growth, morphological and flowering related characters were measured from each sample. Plant height and inflorescence length became longer with delayed transplanting time. Number of leaves including bracts and stem diameter were more and thicker when transplanted on June 30 than two other treatments. Leaves and bracts, stem, root, shoot and total dry weights showed nearly the same result to the number of leaves although floret dry weight were increased as transplanting time was delayed. Although florets were most formed when transplanted on June 30, they were not flowered because of the night-break treatment, meaning that the transplanting time had little effect on its growth and anthesis of the florets.

• Journal of the Korean Geotechnical Society
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• v.33 no.1
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• pp.57-65
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• 2017

During a construction of embankment, sub base, or retaining wall backfill, the speedy measurement of water content is necessary. In this study, a test method for field determination of water content of soil by the calcium carbide gas pressure (speedy water content measurement method) was evaluated for its reliability and accuracy. Dry oven and microwave oven methods were also used for water content measurement. In the test, weathered granite and Nakdong River sand in the site and kaolinite were used for water content measurement. The mass of 20, 22, 24, 26, 28, and 30 g of soil was respectively tested for 1, 3, and 5 min. The effect of each sample on water content was compared one another and analyzed. As the mass and testing time increased, the water content increased. The amount of soil was more important factor than testing time for the speedy water content measurement. In order to obtain similar result to that of dry oven method, 3 min of testing time with 24 g of soil was necessary for weathered granite classified as SM and 3 min with 30 g for Nakdong River sand classified as SP. For Nakdong River sand with 20-50% of kaolinite, the water content by speedy measurement increased as the clay content increased.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.2
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• pp.77-86
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• 2002

Soil color characteristics were measured by a optical instrument, colorimeter, comparing with the conventional Munsell color chart and related to the chemical properties of soils. Total of the 67 experimental soils were taken from tobacco fields that located at Cheongwon, Enmseong, Boeun, Goesan, Jincheon, and Chungju countries in Chungbuk Province having the 29 soil series including Samgag. The values of L, a, and b measured by colorimeter were closely related with hue, value, and chroma of Munsell color chart, respectively, indicating the quantitative measurement of soil color characteristics. The standard deviations in measurement for L, a, and b values was smaller in soil sample passed by 0.5 mm sieve than 2 mm sieve, suggesting that soil particle size less than 0.5 mm was better condition for colorimeter measurement. The values of L and b measured by colorimeter showed a tendency to decrease as increase of soil moisture content but nearly on difference with moisture condition for the value of a. However, correlation coefficient between air dry samples and wet soil samples(soil moisture retension of -10 hPa) for measurements of L, a and b value were more than 0.9 showing the same tendency in measurement. Consequently, air dry soil passed by 0.5 mm sieve was recommended to desirable conditions for stable measurement by colorimeter. The measured values by colorimeter were significantly correlated with organic matter, CEC, exchangeable Ca and Mg, showing the highest correlation coefficient between L value and organic matter.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1612-1612
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• 2001

The production of grain for export and domestic use is one of Australia's most important agricultural industries, and the NIR technique has been used extensively over many years for the routine monitoring of grain quality, particularly moisture and protein content. Because most Australian grain is intended for human food production, the determinants of grain quality for livestock feed, apart from protein, have been largely ignored. However the increasing use of grain for feeding to pigs, poultry, beef cattle and dairy cows has led to an important national research project entitled “Premium Grains for Livestock”. Two of the objectives of this project are to determine the compositional and functional characteristics of grains which influence their nutritional quality for the various classes of livestock, and to adopt rapid and objective analytical tests for these quality criteria. NIR has been used in this project firstly to identify a set of grain samples from a large population of breeders' lines which showed a wide spectral variation, and hence a potentially wide variation in nutritional value. The selected samples were not only subjected to an extensive array of chemical, physical and in vitro analyses, but also were grown out to produce sufficient quantities of grain to feed to animals in vivo studies. Additional grains were also strategically selected from farms in order to include the effect of weather damage, such as rain, drought and frost. In this study to date, NIR calibrations have been derived or attempted, on both ground and whole grains, for in vivo dry matter digestibility (DMD), pepsin-cellulase dry matter disappearance, protein, fat, acid detergent fibre, neutral detergent fibre, starch, in sacco DMD and in vitro assays to simulate starch digestion in the lumen and small intestine. Results so far indicate high calibration accuracy for chemical components (SECV 0.3 to 2.6%) and very promising statistics for in vivo DMD (SECV 1.8, $R^2$ 0.93, SD 7.0, range 61.9 to 92.3, n=60). There appears to be some potential for NIR to estimate some in vitro properties, depending upon the accuracy of reference methods and appropriate sample populations. Current work is in progress to extend the range of grains with in vivo DMD values (a very laborious and expensive process) and to increase the robustness of the various NIR calibrations, with the aim of implementing uniform testing procedures for nutritional value of grains throughout Australia.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1510-1510
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• 2001

NIRS uses reflectance signals resulting from bending and stretching vibrations in chemical bonds between carbon, nitrogen, hydrogen, sulfur and oxygen. These reflectance signals are used to measure the concentration of major chemical composition and other descriptors of homogenized and freeze-dried whole broiler carcasses. Six strains of chicken were analyzed and the NIRS model predictions compared to reference data. The results of this comparison indicate that NIRS is a rapid tool for predicting dry matter (DM), fat, crude protein (CP) and ash content in the broiler carcass. Males and females of six commercial strain crosses of broiler chicken (Gallus domesticus) were used in this study (6$\times$2 factorial design). Each strain was grown to 16 weeks of age, and duplicate serial samples were taken for body composition analysis. Each whole carcass was pressure-cooked, homogenized, and a representative sample was freeze-dried. Body composition determined as follows: DM by oven dried method at 105$^{\circ}C$ for 3 hours, fat by Mojonnier diethyl ether extraction, CP by measuring nitrogen content using an auto-analyzer with Kjeldhal digest and ash by combustion in a muffle furnace for 24 hour at 55$0^{\circ}C$. These homogenized and freeze-dried carcass samples were then scanned with a Foss NIR Systems 6500 visible-NIR spectrophotometer (400-2500nm) (Foss NIR Systems, Silver Spring, MD., US) using Infra-Soft-International, ISI, WinISl software (ISI, Port Matilda, US). The NIRS spectra were analyzed using principal component (PC) analysis. This data was corrected for scatter using standard normal “Variate” and “Detrend” technique. The accuracy of the NIRS calibration equations developed using Partial Least Squares (PLS) for predicting major chemical composition and carcass descriptors- such as body mass (BM), bird dry matter and moisture content was tested using cross validation. Discrimination analysis was also used for sex and strain identification. According to Dr John Shenk, the creator of the ISI software, the calibration equations with the correlation coefficient, $R^2$, between reference data and NIRS predicted results of above 0.90 is excellent and between 0.70 to 0.89 is a good quantifying guideline. The excellent calibration equations for DM ($R^2$= 0.99), fat (0.98) and CP (0.92) and a good quantifying guideline equation for ash (0.80) were developed in this study. The results of cross validation statistics for carcass descriptors, body composition using reference methods, inter-correlation between carcass descriptors and NIRS calibration, and the results of discrimination analysis for sex and strain identification will also be presented in the poster. The NIRS predicted daily gain and calculated daily gain from this experiment, and true daily gain (using data from another experiment with closely related broiler chicken from each of the six strains) will also be discussed in the paper.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.3
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• pp.351-359
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• 2015

Twelve multiparous Holstein dairy cows in mid-lactation were selected for a replicated $4{\times}4$ Latin square design with a $2{\times}2$ factorial arrangement to investigate the effects of corn and soybean meal (SBM) types on rumen fermentation, N metabolism and lactation performance in dairy cows. Two types of corn (dry ground [DGC] and steam-flaked corn [SFC]) and two types of SBM (solvent-extracted and heat-treated SBM) with different ruminal degradation rates and extents were used to formulate four diets with the same basal ingredients. Each period lasted for 21 days, including 14 d for adaptation and 7 d for sample collection. Cows receiving SFC had a lower dry matter (DM) and total N intake than those fed DGC. However, the milk yield and milk protein yield were not influenced by the corn type, resulting in higher feed and N utilization efficiency in SFC-fed cows than those receiving DGC. Ruminal acetate concentrations was greater and total volatile fatty acids concentrations tended to be greater for cows receiving DGC relative to cows fed SFC, but milk fat content was not influenced by corn type. The SFC-fed cows had lower ruminal ammonia-N, less urea N in their blood and milk, and lower fecal N excretion than those on DGC. Compared with solvent-extracted SBM-fed cows, cows receiving heat-treated SBM had lower microbial protein yield in the rumen, but similar total tract apparent nutrient digestibility, N metabolism measurements, and productivity. Excessive supply of metabolizable protein in all diets may have caused the lack of difference in lactation performance between SBM types. Results of the present study indicated that increasing the energy degradability in the rumen could improve feed efficiency, and reduce environmental pollution.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.4
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• pp.530-538
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• 2016

Two experiments were conducted to determine the nutrient composition, in vitro ruminal ammonia concentrations and pH of wet distillers grains (WDG, produced from tapioca 70% and rice 30%) and to evaluate dietary effects of fermented total mixed ration (TMR) using WDG on the performance, blood metabolites and carcass characteristics of Hanwoo steers from mid fattening to slaughter. In Exp. I, average dry matter (DM), crude protein, ether extract, crude fiber, ash, neutral detergent fiber, acid detergent fiber, and nitrogen free extract of seven WDG samples from an ethanol plant with different sampling dates were 19.9%, 24.8%, 3.8%, 21.8%, 8.87%, 60.3%, 34.5%, and 40.7% (DM basis), respectively. For in vitro ammonia concentrations and pH, each sample was assigned to 7 incubation times (0, 4, 8, 12, 24, 48, and 72 h). Linear increase was observed between 12 and 48 h for ammonia concentrations, but final ammonia concentrations (72 h) were not significantly different among WDG samples and fermentation patterns of WDG samples showed similar tendency. In vitro pH varied among treatments from 0 to 24 h, but were not different statistically after 48 h. In Exp. II, 45 Hanwoo steers of 23 months ($641{\pm}123kg$) from mid fattening period to slaughter (248 days) were randomly divided into three groups of 15 pens each (five repetitions/each treatment) and assigned to one of three dietary treatments; i) Control (TMR), ii) WDG 15 (TMR containing 15% of WDG, as fed basis) and iii) WDG 28 (TMR containing 28% of WDG, as fed basis). The body weight (BW), ADG, and feed conversion ratio (FCR) of control and WDG 15 and 28 during 248 days were 760.8, 740.1, and 765.5 kg, and 0.50, 0.50, and 0.52 kg/d, and 18.6, 17.6, and 17.1, respectively. The dry matter intake (DMI) (kg/d) of control (9.11) was higher (p<0.05) than WDG treatments (WDG 15%, 8.57; 28%, 8.70). Nevertheless, DMI did not affect BW, ADG, and FCR of Hanwoo finishing steers. Blood metabolites were in normal ranges and were not different among treatments except the albumin concentration. In carcass characteristics, WDG 15 (30%) showed higher frequency of A-carcass yield grade than WDG 28 (15%) and control (7%), and WDG 28 (61%) showed higher frequency of $1^{{+}{+}}$ and $1^+$-carcass quality grade than WDG 15 (40%) and control (60%). In conclusion, using WDG up to 28% in TMR did not show any negative effect on the performance and blood metabolites, and improved carcass quality of Hanwoo steers. Therefore, WDG can be a useful feed ingredient for Hanwoo steers in mid-fattening period to slaughter.

• Korean Journal of Food Science and Technology
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• v.30 no.5
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• pp.1018-1023
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• 1998

Hydrocarbons in dry soup base and its ingredients in instant noodle were analyzed to determine whether the analysis of hydrocarbons is a potential method to detect post-irradiation of the soup base. Soup base ingredients were irradiated individually, irradiated before mixing, or mixed after irradiation. Lipids were extracted with hexane and hydrocarbons were separated from the lipids through Florisil column. The hydrocarbons were analyzed with GC. Hydrocarbons C17:2, C16:3, C17:1, and C16:2 were detected in palm oil, red pepper powder, and sesame seeds irradiated at 10 kGy, but not in unirradiated ones. C17:2, C16:3, C17:1, and C16:2 were not detected in the soup base mixture of unirradiated ingredients. The four hydrocarbons were detected in the soup base mixture using irradiated palm oil or sesame seeds. In the mixture using irradiated red pepper powder, C17:2 and C16:3 were detected. When the soup mixture was irradiated after mixing unirradiated ingredients, C17:2, C17:1, and C16:2 were detected in the sample irradiated at 1 kGy, and C17:2, C16:3, C17:1, and C16:2 were in large amounts at 5 and 10 kGy.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.4
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• pp.303-308
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• 2001

To investigate the partitioning of newly absorbed N derived from NO$_3$- and NH$_4$$^{+}$, 6 mM $K^{15}$ NO$_3$ or 3 mM ($^{15}$ NH$_4$)$_2$ was fed continuously in Italian ryegrass (Lolium multiflrum L.) for 7 days. Nitrogen metabolites (nitrate, amino acid, soluble- and insoluble protein) were analyzed at the end of $^{15}$ N feeding. Dry weight in shoot, stubble and root was not significantly different between NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ feeding. Total nitrogen content in all three organs was significantly higher in NH$_4$$^{+}$ than NO$_3$$^{[-10]}$ feeding. Sum on N content in reduced N fractions (amino acids + proteins) in shoot, stubble and roots in NH$_4$$^{+}$ feeding increased by 13.3, 12.5 and 35.4 %, respectively, compared to NO$_3$$^{[-10]}$ feeding. The Relative Specific Activity (RSA, percentage of newly absorbed $^{15}$ N relative to total N in a sample) values of amino acids and insoluble proteins were significantly higher in NH$_4$$^{+}$ feeding. Total amount of newly absorbed $^{15}$ N in NO$_3$$^{[-10]}$ and NO$_3$$^{[-10]}$ feeding was 52.3 and 69.5 mg/plant on dry matter basis, respectively. In both NH$_4$$^{+}$ and NO$_3$$^{[-10]}$ grown plants, most of the N was allocated to the shoot, 67.5% in NH$_4$$^{+}$ feeding and 58.8% NO$_3$$^{[-10]}$ feeding, respectively. The $^{15}$ N amount incorporated in the reduced N compounds (amino acids and proteins) in NH$_4$$^{+}$ grown plants significantly increased by 74.8% compared to NO$_3$$^{[-10]}$ grown plants. The increase of the $^{15}$ N amount assimilated to amino acids in NH$_4$$^{+}$ grown plants was remarkably higher in roots as more than 7.25 times compared to NO$_3$$^{[-10]}$ feeding. These results indicated that Italian ryegrass was much efficiently utilized NH$_4$$^{+}$-N for the synthesis of reduced N compounds.reduced N compounds.