• 한국식물병리학회지
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• 제3권1호
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• pp.13-16
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• 1987

Infection rate of chestnut tree by Botryosphaeria(Dothiorella) dathidea was about $10\%$ at Gapyung area. disease symptom of chestnut did not appear in October but appeared in November, showing $50\%$ in May in ext year. B. dothidea was more pathogenic to chestnut than did Catinula sp. and Fusicoccum sp. B. dothidea uses soft rot, but Catinula sp. and Fusicoccum sp. causes dry rot. Mycelial growth of B. dothidea was optimal at $28^{\circ}C$. Mycelial growth was stimulated when water potential decreased from -2 bars to -12 bars and declined from -12 bars to -60 bars.

• 농약과학회지
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• 제11권4호
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• pp.331-338
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• 2007

마늘 저장중의 부패를 일으키는 주요 진균인 푸른곰팡이병균(Penicillium hirsutum)과 마름썩음병균(Fusarium oxysporum)의 생장을 억제하는 농약을 선발하고자 살균제 11종을 선정하여 균사생육억제시험과 접종시험을 수행하였다. 균사생육억제 정도와 마늘인편에서의 발병 억제 효과를 비교하기 위하여 시험약제를 처리한 후 병원균을 접종하여 병 발생정도를 조사한 결과, F. oxysporium에 대하여는 diphenylamine, prochloraz와 tebuconazole을 처리한 구에서는 초기에 발병이 현저히 억제되었으며, 기준량 처리시 접종 5일후 부터 병 발생이 시작되어 11일 후에는 $73.3{\sim}100%$ 발생하였다. P. hirsutum의 경우 cyprodinil, prochloraz 및 pyrimethanil을 기준농도 처리한 구에서 접종 5일 후부터 발병하였으며, diphenylamine, penconazole 및 propiconazole 처리구에서는 7일 후부터 발병하여 11일 후에는 $20{\sim}100%$ 정도 발생되었으나 tebuconazole 처리구에서는 병 발생이 되지 않았다. 이상의 결과를 종합하여 볼 때 diphenylamins, prochloraz와 tebuconazole 이 마름썩음병 및 푸른곰팡이병의 방제에 효과가 있는 것으로 확인되었으며, 약제별 방제가와 균총의 생육억제에 대한 상관분석결과, F. oxysporum에 대하여는 diphenylamine과 prochloraz가 높은 상관관계가 있었고, P. hirsutum에 대하여는 cyprodinil, prochloraz와 pyrimethanil이 높은 상관관계를 유지하였다.

• 한국균학회지
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• 제42권1호
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• pp.91-94
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• 2014

2013년 6월, 경기도 여주시에서 저장 중인 고구마 괴근 표면에 갈색의 썩음 증상이 관찰되었다. 병징은 둥글고, 옅은 갈색 내지 진한 갈색이며, 단단하고 건조한 편으로 표피에 한하여 얄팍하게 나타났다. 병반 부위는 오래된 부위에서 움푹 패이고, 괴근의 내부로 깊게 썩히지 않았다. 병든 고구마에서 7개의 Fusarium균을 분리하였고, CLA 배지에서 균학적 특징을 조사한 결과 F. oxysporum으로 동정되었다. 이러한 결과를 확인하기 위하여 elongation factor 1 ${\alpha}$ 유전자 부위의 염기서열을 분석하였다. 그 결과, 7개 균주 중 4개는 F. commune으로, 3개는 F. oxysporum으로 확인되었다. 고구마 괴근을 상처구와 무상처구로 구분하고, 균사절편을 접종하여 병원성을 확인한 결과, 접종 7일 후 F. commune을 접종한 상처구에서만 병징이 관찰되었다. 따라서 본 병을 Fusarium commune에 의한 고구마 표피썩음병으로 명명하며, 국내에서 최초로 보고한다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2005년도 한국식물병리학회 임시총회 및 춘계학술발표회
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• pp.51.1-51.1
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• 2005

• 한국약용작물학회지
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• 제27권4호
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• pp.284-291
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• 2019

Background: Ginseng root rot disease, caused by Cylindrocarpon destructans and Fusarium solani is a major cause of replant failure in continuous cropping ginseng. Methods and Results: To control replant injury in soil infected with C. destructans and F. solani, biosolarization was performed by covering the plot with transparent polyethylene film after adding green manure of maize and sunflower for the summer season. Per 10 a, fresh and dry weight of maize was 10.1 and 2.5 tons, respectively, and that of sunflower was 8.1 tons and 1.2 tons, respectively. Mean maximum temperature at 20 cm depth was $33.2^{\circ}C$, $41.5^{\circ}C$ and $41.8^{\circ}C$ in the control, maize-incorporated and sunflower-incorporated plots, respectively. The elapsed time over $40^{\circ}C$ was 36.4 h in the maize-incorporated plot and 77.3 h in the sunflower-incorporated plot. Biosolarization increased $NO_3$ content in soil, while content of organic matter, Ca, and Mg was decreased. Electrical conductivity, $NO_3$ and $P_2O_5$ in soil significantly increased after two years of biosolarization. The number of spores of C. destructans in soil was significantly decreased by biosolarization, and sunflower treatment was more effective than maize treatment in decreasing the number of spores. Root yield of 3-year-old ginseng was significantly increased by biosolarization, however, there was no significant difference between maize and sunflower treatments. Rate of root rot in 3-year-old ginseng decreased to 16.5% with the incorporation maize and 5.0% with the incorporation of sunflower, while that in control 25.6%. Conclusions: Biosolarization was effective in inhibiting ginseng root rot by decreasing the density of root rot disease and improving soil chemical properties.

• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.483-489
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• 2008

Nine newly isolated mushroom strains were tested to assess both their zinc tolerance and potential for zinc removal from an aqueous solution. Four strains of ectomycorrhizal fungi, namely Clavariadelphus truncatus (T 192), Rhizopogon roseolus (T 21), Lepista nuda (T 373), and Tricholoma equestre (T 174), along with five strains of white rot fungi, Lenzites betulina (S 2), Trametes hirsuta (T 587), Ganoderma spp. (T 99), Polyporus arcularius (T 438), and Ganoderma carnosum (M 88), were investigated using zinc-amended solid and liquid media. Their biosorption properties were also determined. The colony diameter and dry weight were used as tolerance indices for fungal growth. C. truncatus and T. equestre were not strongly inhibited at the highest concentrations of (225 mg/l) zinc in solid media. The most tolerant four strains with solid media, C. truncatus, G carnosum, T. hirsuta, and T. equestre, were then chosen for tolerance tests in liquid media. An ectomycorrhizal strain, C. truncatus, was also detected as the most tolerant strain in liquid media. However, the metal-tolerant strains demonstrated weak activity in the biosorption studies. In contrast, the highest biosorption activity was presented by a more sensitive strain, G. carnosum. In addition, seven different biosorbent types from G. carnosum (M 88) were compared for their Zn (II) biosorption in batch experiments.

• Mycobiology
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• 제38권1호
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• pp.39-45
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• 2010

White rot, which is caused by Sclerotium cepivorum, is a lethal disease affecting green onions. Three different types of nanosilver liquid (WA-CV-WA13B, WA-AT-WB13R, and WA-PR-WB13R) were tested in several different concentrations on three types of media to assess their antifungal activities. Results from in vitro experiments showed that all three of the nano-silver liquids had more than 90% inhibition rates at a concentration of 7 ppm. Greenhouse experiments revealed that all of the nano-silver liquids increased biomass and dry weights, and there were minimal changes in the population of various bacteria and fungi from the soil of greenhouse-cultivated green onions. In addition, a soil chemical analysis showed that there were minimal changes in soil composition.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.90.2-91
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• 2003

In commercial greenhouses, senescent flower petals or flowers of vegetables such as tomato, strawberry, hot pepper and zucchini squash were blighted to be removed from fruits within five days after spraying of Trichoderma harzianum YC459 (TORY), a biocontrol agent for the gray mold rot of vegetables caused by B. cinerea The mechanism for selective colonization of senescent floral tissues by T. harzianum YC459 was elucidated using fresh and senescent (Hays and 14days after flowering, respectively) floral tissues of zucchini squash (Cucurbita moschata Duchesne). The spores of T. hrzianum YC459 were produced more on agar and liquid culture media supplemented with 5％ dry powder of senescent floral tissues than fresh tissues during 15days. Mycelial growth was also much better in the media with senescent tissues than with fresh tissues. Enzyme activities of amylase, polygalacturonase and cellulase in the liquid media which might be involved in the colonization of tissues by T. harzianum YC459 were compared. The activities of three enzymes were much higher in the media with senescent floral tissues than with fresh floral tissues reaching to the maximum during 9 to 12days of incubation. Based on the results, the removal of senescent floral tissues, a possible inoculum source of the pathogen, may be another mechanism for biocontrol of gray mold rot of vegetables by T. harzianum YC459.

• The Plant Pathology Journal
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• 제17권5호
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• pp.257-263
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• 2001

Species of the genus Orobanche are parasitic flowering plants, holoparasites, which cling to the roots of green plants. Their tiny seeds (200 x $250\mu\textrm{m}$) germinate in response to chemical stimuli produced by host and some non-host plants. Successful contact with their host leads to development of haustoria for obtaining water and food. The shoots above the ground expose flowers and disseminate seeds. Several samples of Orobanche ramosa, O. crenata, O. cernua, and O. egyptiaca were collected from different localities in Jordan. These samples showed one of the following disease symptoms: dry rot at the base of the stem; general deterioration and expanded lesion from base upward; soft tissue maceration of stem; and black rot of flower parts with incomplete maturation of the ovary and seeds. Isolation from diseased stems and seeds was made on three different mycological media. Several fungi were isolated, mainly, Fusarium spp., Alternaria alternata, Rhizoctonia sp., Dendrophora sp., Chaetomium sp., and an ascomycetus fungus with a perithecium. Pathogenicity tests showed that Fusarium spp. and Alternaria alternata attacked healthy living tissue of Orobanche spikes. These fungi caused lesions of black soft rot and complete deterioration within 5-7 days. They also attacked Orobanche seeds, arresting their germination and causing maceration of non-germinated and germinated seeds after 5-7 days of incubation. Meanwhile, Dendrophora sp. and Chaetomium sp. caused limited lesion at first, but were able to colonize the tissue as it aged and senesced. This study showed the presence of a potential endogenous pathogenic fungi in Jordan, which can be investigated as a biological control for Orobanche.

• 식물병연구
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• 제12권3호
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• pp.158-163
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• 2006

시설재배 되는 들깨에 발생하여 피해를 초래하는 균핵병 발생 특성에 대하여 조사연구한 결과, 들깨 균핵병균(S. sclerotiorum)의 균사생장 적온은 $20^{\circ}C$이었고, $15^{\circ}C$ 이하의 온도에서 $20^{\circ}C$ 이상의 온도에서보다 균핵의 형성수는 적었으나 보다 큰 균핵을 형성하였으며 건물중도 높았다. 토양 깊이별 균핵의 자낭반 형성은 0.5 cm에서 가장 높았고 3cm 깊이까지 자낭반을 하였으나 5cm 이상의 깊이에서는 자낭반 형성이 관찰되지 않았다. 들깨 균핵병은 전 생육기에 걸쳐 발생하였고, 특히 저온 다습기인 1월에서 2월에 발생이 높았다. 그리고 5년 이상 연작 재배시 높은 발병을 보였으며, 10년 이상 연작재배 포장은 20% 이상의 발병을 보여 많은 피해를 초래하였다. 또한 들깨 시설재배 농가 포장에서 자연 감염에 의한 균핵병이 떡쑥, 주름잎 등의 잡초에 발생하였으며, 병원성 조사결과, 화본과를 제외한 국화과 등 6개과에 속하는 11종의 잡초에 병원성을 나타내었다. 이런 결과는 윤작과 효율적인 잡초관리가 들깨 균핵병 발생을 경감할 수 있고, 벼 후작으로 시설재배 되는 들깨에 균핵병이 격발하는 원인을 설명할 수 있는 하나의 자료가 될 수 있을 것으로 생각된다.