• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.1
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• pp.30-36
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• 1995

Pasture composed of about 30% botanical composition of white clover (Trijolium repem L.) is desirable in its productivity and quality. To get information on maintaining its productivity in the pasture, the experiment was canied out to determine the effect of infrequent, frequent defoliation or their alternation on change of its harvest yield and growth. Individual plants of Regal, Louisiana S-1, Grasslands Huia and Aberystwyth S184 were grown in 22cm plastic pot containing a 2 : 1 : 1 soil : sand : Peat moss mixture for 27 days after transplanting 50day seedlings raised on 3cm pots, and then all their fully expanded leaves are removed. Defoliation treatments were forced every 1 (CC, frequent), 4 week (RR, infrequent) or their alternations (CR, RC) after 8 weeks from the removal. To analyze the treatment effects, plants were sampled on 0 (the removal day), 4, 8, 12 and 16 weeks after the removal and seperated to leaves, petioles, stolons and roots. Each harvest yield of infrequent defoliation (RR) was higher than that of frequent defoliation (CC). Their alternations (RC, CR), however, forced fluctuation of the yield according to defoliation interval. Fraction weights from their altemations showed intermediate ones of infrequent and Frequent defoliation whose weights did the similar result to their harvest yields. Fraction and total dry weights per plant, shoot/root ratio were changed by relative span of defoliation interval, which resulted from the weights of leaves and petioles, removed parts. Root and total dry weight per plant of Regal were greater than the other cultivm in infrequent defoliation interval regardless of continuous or alternations. Productivity of white clover in pasture, therefore, can be controlled by alternation of infrequent and frequent defoliation or reverse.

• Korean Journal of Pharmacognosy
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• v.18 no.3
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• pp.196-201
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• 1987

A study was carried out to investigate the possibility of acclimation of Coptis japonica Makino in Korea. Coptis japonica could be grown within 4 years as a medicinal crude drug if it is cultivated in the area of mountain facing north(300m above sea level) with 70% light interception and appropriate soil texture. It is to be noted that the propagation of the plant is more important than the yield of berberine because the berberine contents do not change in different conditions. The results were as follows; 1. The germination rates of the seeds by covering with the pine tree leaves were the highest and averaged 63%. 2. The germination rates of dry seeds were not affected in spite of low temperature and $GA_3$ treatment. 3. The germination rates of the seeds stored in moistured sand were averaged 85%, but in dry state, they were not germinated. 4. The number of leaves and fresh weight of Coptis japonica from the area of mountain facing north were increased significantly. 5. The growth and fresh weight of Coptis japonica were reduced in clay. 6. The best growth was obtained under 70% light interception. It is possible to intercrop between evergreen trees. 7. The foliage dressing did not affect the growth. 8. The number of leaves and fresh weight were increased from the 3rd year, and the average weight of rhizomes was 5.7g in 4th year. 9. The berberine contents of the rhizomes of Coptis japonica under various treatment conditions ranged from 9 to 10%. Coptidis Rhizoma from this study and from Korean market showed much higher berberine contents than those from Japanese market.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.2
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• pp.118-122
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• 2006

This experiment was carried out on plastic pots ($40cm{\times}25cm{\times}30cm$) filled with sand soil at greenhouse using two soybean cultivars with small seed; one was Pungsannamulkong (PSNK) recognized as a tolerant cultivar against excessive water stress and the other one was Sobaeknamulkong (SBNK) recognized as a susceptible cultivar. Seed was sown with 30 plants of 2 hills, and the amount of applied fertilizer was N; 3.0 g, P; 3.0 g, and K; 3.4 g per $m^2$ with all basal fertilizations. Plants were grown under photoperiod of natural light with day temperature of $31{\pm}5^{\circ}C$ and night temperature of $22{\pm}1^{\circ}C$. The flooding treatment was done for 3, 5, 7 and 10 days by filling pots with tap water up to 1 cm above the level of the soil surface when plants were 2 days after emerging. Nitrogen uptake by leaves of soybeans decreased significantly by the flooding after 6 days. This significant reduction of N uptake by flooding was evidently recognized from the chlorosis of leaves. The dry matter of flooded soybean seedlings significantly decreased compared to non-flooded soybean seedlings at 10 days. The dry matter of roots also showed similar result of the shoot. Shoots had more N reduction than roots under the flooding. This N reduction was more pronounce in SBNK than in PSNK. Chlorophyll content of flooded soybeans showed decreasing or non-increasing tendency, and the reduction of chlorophyll content was more in SBNK than in PSNK from the flooding stress. Nitrate content of soybean seedlings with flooding stress showed decreasing tendency in shoot and root parts. Ammonium content, however, was higher in flooding stress compared to the non-flooding. Flooding caused a remarkable change in the AA (amino acid) composition and TAA (total amino acid) concentration in the leaves of soybean seedlings.

• Journal of Life Science
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• v.11 no.2
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• pp.166-172
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• 2001

Various types of the seeding of bottle gourd widely used as a rootstock of watermelon has been required to satisfy the farmers need. The study was done to determine the effect of light quality of blue, red and far-red lights treated with light emitting diodes on growth and morphology of bottle goured seeding and chlorophyll content of its cotyledons. The lights were treated in the growth chamber for 7 days to the seeding elapsed 8 days after sowing under natural condition, and 64 hole trays with commercial bedsoil. Plant height, length and diameter of hypocotyl, leaf area of cotyledon and first true leaf, its leaf length, number of true leaves, fraction and total dry weight were measured. Red light shortened and slenderized the hypocotyl, which lengthened by far-red light and thickened by blue light. Plant height was declined in order of far-red light treatment, blue and red lights mainly due to difference of hypocotyl length, Area and length of the first true leaf became smaller and shorter under far-red light than under the other lights. However, blue light increased leaf area of cotyledons. Two cultivars cv. Yongjadaemok and cv. Kunghap had different response to the light treatments in total seedling dry weight(dw); far-red and red light treatments showed the greatest and the least dw of hypocotyl, respectively, while blue and red lights did the greatest dw of the other organs. Among the ratio of each organ dw to total dw, those of hypocotyl and true leaves were different between the light treatments; the highest ratio of hypocotyl dw to total dw was observed in far-red light treatment but the lowest was in red light treatment. Those of the true leaves were the lowest in far-red light and similar response in blue or red light treatment. Chlorophyll content of cotyledons was decreased in order of red light treatment, blue and far-red lights, meaning that short period light treatment may influence photosynthesis of seeding and afterward its growth.

• Journal of Life Science
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• v.11 no.6
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• pp.612-617
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• 2001

Seedlings of red pepper (Capsicum annum L.) were exposed to various doses of ${\gamma}$-radiation )($^{60}$ Co)and cultivated in the 1/2,000 a pot. Plant hight the number of leaves, flowers and fruits, chlorophyll contents, plant dry weight, ac- tivites of antiozidant enzymes such as superoxide dismutase(SOD) adn peroxidase(POD), were determined. Plant height of seedling treated with 1 Gy of ${\gamma}$-radiation was increased up to 16.6% as compared to untreated control at 80 days after treatment, while those treated with 20 Gy or 50 Gy was decreased 22.0% or 75.0% respectively. The plants treated with 1 Gy or 5 Gy were increased the number of leaves up to 41.2% and 24.9% as well as dry weight of fruit up to 58.4% and 49.4% respectively. However treatment of radiation higher than 20 Gy significantly inhibited the growth, chlorophyll content and yield of pepper fruit. Activities of antioxidant enzymes, SOD and POD were tem-porary increased within one or five days after treatment depending on the doses of treatment , and the activities were gradually decreased to control level of lower thereafter. The SOD and POD activities in the leaves of pepper plant treated with 50 Gy were 5.5 and 6.0folds higher than that of control at five days after treatment, respectively.

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.265-269
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• 2008

The antioxidant activities of the extracts of butterbur (Petasites japonicus) derived from different extraction methods were investigated. SOD (superoxide dismutase)-like activity differed according to the extraction solvents, showing a greater antioxidant effect with ethanol solvent than that of water. Ethanol extracts of butterbur leaves showed higher SOD-like activity of 96.7% than those of water extracts. The contents of polyphenolic compounds were higher in water extracts than those in ethanol extracts. The highest content of polyphenolic compounds was 223mg/g dry weight for butterbur leaves. EDA of butterbur roots was 61.5% in the water extract and EDA in butterbur leaves was 34.9% in the ethanol extract. The anticancer effects with the extracts of butterbur were experimented by fractionations with different solvents. 41.9% of the growth of stomach cancer cells, SNU-719, were inhibited and also 72.7% of the growth of liver cancer cells, Hep3B, were inhibited by the butanol fractions of butterbur, while not affecting the growth of normal cell, DC 2.4.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.6
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• pp.582-587
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• 2015

This study was conducted to compare the plant growth and fruit quality of blueberries grown in different soil textures of Korea, in order to utilize the results for stable production and soil improvement. Rabbiteye blueberry cultivars 'Tifblue' and 'Baldwin' were planted and grown for three years from 2013 in wagner pot (1 $2000a^{-1}$) in a greenhouse of Namhae Sub-station, Institute of Horticultural and Herbal Science. The plants were grown in four soil textures, sand, sandy loam, loam and silt loam, and nutrient uptake and growth characteristics of plants were investigated. Leaf nitrogen and phosphorus contents of two cultivars grown in different soil textures ranged between 8.6 to $10.5gkg^{-1}$, which was lower than appropriate level for rabbiteye blueberry. However, the contents of potassium, calcium and magnesium in leaves were appropriate levels as $2.29{\sim}3.62gkg^{-1}$, $4.46{\sim}5.46gkg^{-1}$ and $1.45{\sim}2.12gkg^{-1}$, respectively. Nitrogen and phosphate contents in leaves were higher in the two cultivars grown in silt loam soil. There was no significant difference in plant volume and root dry weight among four soil textures in two cultivars. However, dry weight of leaves and branches were highest in loam soil. Fruit production was highest in loam and silt loam soil in two cultivars, showing negative correlation with the amount of sand in soil. However, sugar and acidity showed no correlation with sand content in soil. These results show the limit to the blueberry growth in soil that has no nutrient holding capacity; however, most of Korean soils that have good nutrient holding capacity can produce competitive fruits if the drainage is improved.

• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.493-500
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• 1998

To investigate the effects of light intensity on the growth, and the critical minimum light intensity for growing of Cornus controversa seedlings at the stage of cotyledon, hypocotyl elongation, cotyledon expansion, the times of leaves appearance, dry weights of each organ, and specific leaf area(SLA) were measured on a growth chamber with several light intensity gradients(385, 32, 17, 8, and $5{\mu}mol\;m^{-2}s^{-1}$). There was a positive correlationship between the size of cotyledon and the biomass of cotyledon and total seedling. Hypocotyl was more elongated under relatively low light intensities, such as 32, 17, 8, and $5{\mu}mol\;m^{-2}s^{-1}$ than under $385{\mu}mol\;m^{-2}s^{-1}$ light intensity, however, dry weight of the hypocotyl was adverse. As the light intensities decreased, the leaf appearance was delayed and the number of leaves decresed. In addition, leaves did not appear under $8{\mu}mol\;m^{-2}s^{-1}$ and $5{\mu}mol\;m^{-2}s^{-1}$ light intensity. Although cotyledons were more fully expanded under 32 and $17{\mu}mol\;m^{-2}s^{-1}$ light intensities than $385{\mu}mol\;m^{-2}s^{-1}$ light intensity, the dry weights of cotyledons were greater under the high light intensity. The dry weight of cotyledon, hypocotyl, root and leaves showed a decreased pattern with decreasing light intensities, but root to shoot(hypocotyl+leaves) ratio rapidly increased. Roots did not develop below $8{\mu}mol\;m^{-2}s^{-1}$ light intensity. In conclusion, the results showed that the critical minimum light intensity for growing of Cornus controversa seedlings was above $17{\mu}mol\;m^{-2}s^{-1}$ light intensity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.1
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• pp.23-28
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• 2011

Resveratrol has been associated with reduced cardiovascular disease and reduced cancer risk. This phytochemical has been reported in a number of plant species including grapes, peanuts and pine trees in response to stress such as fungal infection, heavy metal ions or UV irradiation. The objective of this study was to determine the resveratrol contents in leaves of mulberry varieties at different collecting times. Quantitative analysis of 16 cultivars showed a range of $102{\sim}466{\mu}g/100g$ on dry weight basis (which is equivalent to $25{\sim}116.5{\mu}g/100g$ on fresh weight basis). Resveratrol contents in mulberry leaves was higher in autumn than spring, and higher in fully matured leaves than in juvenile leaves. Among the tested samples, 'Kaeryangppong', 'Sugeppong' and 'Cheongilppong' collected in the middle of October showed high resveratrol contents of $838{\mu}g/100g$, $803{\mu}g/100g$, $800{\mu}g/100g$ on dry weight basis, respectively. Especially mulberry leaves dried in the shade showed of $1,030{\mu}g/100g$ on dry weight basis in resveratrol content, this result may contribute to utilization of mulberry leaves.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.5
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• pp.530-537
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• 1998

The proportion of dry matter (DM) and mineral elements (Ca, Mg, P, Na, K, Zn) released from eight feeds (2 rice straws, RSI and RS2; 2 grasses, NB21 and guinea; 2 leguminous fodders, glyricidia and erythrina; jak leaves and rice bran) were studied using the nylon bag procedure. Bag incubations up to 10 days were performed in the rumen of cows fed on a ration consisting of 50% wheat straw and 50% hay. Both the type of feed and the incubation time in the rumen significantly influenced (p < 0.01) the proportion of minerals released. In legumes, jak leaves and rice bran about 80% of the potentially degradable DM fraction was solubilized within 24 h in the rumen, and with the grasses, rice straws and jak leaves a considerable proportion of DM was released between 48 and 240 h in the rumen. During the early hours of incubation (up to 24 h) there were distinct differences between and within the feed classes in their ability to release all mineral elements studied. In all test feeds, high proportions of Mg and K were released within 24 h. Some feeds showed a tendency to ad/absorb Ca (grasses, rice straws and rice bran), P (jak leaves, rice straws), Na (glyricidia and rice bran) and Zn (jak leaves) from water and rumen fluid, and this was partly related to the low initial concentration. In terms of absolute quantity of mineral released, legumes (erythrina is superior to glyricidia) are a good source of Ca, Mg, P and Zn, and jak leaves a good source of Ca and Na. Within grasses, guinea contains appreciable quantity of available Mg and P. Rice bran is rich in available Mg, P and Zn.