• Title/Summary/Keyword: Dry ash method

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Isolation of Immunomodulatory Antitumor Active Polysaccharide (RGAP) from Red Ginseng By-Product and Its Physico-chemical Properties (홍삼추출잔사로부터 항암면역조절 활성을 보여주는 홍삼산성다당체(RGAP)의 분리 및 이화학적 특성)

  • Kwak, Yi-Seong;Shin, Han-Jae;Song, Yong-Bum;Park, Jong-Dae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.5
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    • pp.752-757
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    • 2003
  • This study was carried out in order to develop the method for isolation of red ginseng acidic polysaccharide (RGAP) haying immunomodulating antitumor activity from red ginseng by-product. The red ginseng by-product was obtained from red ginseng residues produced in processing of red ginseng ethanol extract. The yield of RGAP isolated by ultrafiltration was 20.9%. The active substance (GFP) was purified by DEAE-sepharose column chromatography RGAP induced nitric oxide (NO) exhibited tumoricidal activities against P8l5 (mastocytoma) tumor cells. Acid-hydrolyzed RGAP fragments were shown four to five spots. These sopts showed the same R$_{f}$ values with sugars designated as rhamnose, glucose, glactose and glucuronic acid. Some physico-chemical properties of RGAP were investigated. pH and dry reduction content at 105$^{\circ}C$ were 4.74 and 4.72%, respectively. Crude protein, ash and Pb contents were 3.30%, 4.74% and 2.30 ppm. These results suggest that we will be able to produce RGAP from red ginseng by-product by ultrafiltration in a large scale.e.

Assessment of Bond Characteristics between New and Old Concrete in Various Mixtures and Joint Conditions (배합 및 접합면 처리에 따른 신·구 콘크리트의 부착특성 평가)

  • Cho, Byeong-Du;Kim, Sang-Hyun;Jeon, Se-Jin
    • Journal of the Korea Concrete Institute
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    • v.26 no.4
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    • pp.507-515
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    • 2014
  • Although the construction joints of a concrete structure are properly treated with some measures, leakage has frequently occurred. A series of tests on the bond characteristics between new and old concrete were carried out in this study, assuming that the leakage at the construction joints has certain relationship with the bond of concrete. To assess the bond characteristics under various conditions, a number of specimens were made that have an interface between new and old concrete and bond strength, flexural strength and splitting tensile strength were measured. Main test variables are type and amount of mineral admixtures, treatment method of the interface and type of waterstops. In addition, the effects of placing interval between the concrete and of the age of the strength tests were investigated. The test results showed a slightly increased bond strength when applying mineral admixtures, which can be attributed to the interface filled with the calcium silicate hydrate that is formed by pozzolanic reaction. On the other hand, the bond strength was higher when the interface was treated rough and dry, and the roughness of a waterstop affected the bond capacity of the waterstop. Also, an assessment is required that considers the type of strength test because the bond strength varied according to the test methods.

Physiological Function in vitro of ${\beta}-Glucan$ Isolated from Barley (보리가루에서 분리한 ${\beta}-Glucan$의 생리적 기능성)

  • Oh, Hee-Jung;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.28 no.4
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    • pp.689-695
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    • 1996
  • In order to prove physiological function of ${\beta}-Glucan$ isolated from barley flour by enzymatic method, in vitro experiments simulating the passive membrane transport of gastrointestinal tract were carried out using dialysis membrane. The yield of ${\beta}-Glucan$ from barley flour was $6.2{\%}$ and its constituents were determined to give $81.6{\%}$ total dietary fiber, $72.9{\%}$ soluble dietary fiber, $8.7{\%}$ insoluble dietary fiber, $8.5{\%}$ moisture, $2.5{\%}$ protein and $7.4{\%}$ ash. The water holding capacity of the ${\beta}-Glucan$ preparation was 6 g water/g dry material. The glucose retardation index after 30 minute dialysis was $13.5{\%}$ in the presence of $3{\%}$ ${\beta}-Glucan$. As the dialysis period became longer, the retarding effect toward glucose absorption decreased and the effect was close to zero after 2 hour dialysis. The bile acid retardation index after 30 minute dialysis was 3, 12 and $18{\%}$ in the presence of 1, 3 and $5{\%}$ ${\beta}-Glucan$, respectively. The effect was higher than the glucose retardation index and decreased as the dialysis time elapsed.

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Physical Properties of Chitosan Film made from Crab Shell (꽃게 껍질에서 분리제조한 키틴산 필름의 물성에 관한 연구)

  • Cho, Jeong-Suk;Han, Jeong-Jun;Lee, Cherl-Ho
    • Korean Journal of Food Science and Technology
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    • v.24 no.6
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    • pp.574-580
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    • 1992
  • Chitin was isolated from the residue of enzymatically hydrolyzed crab, Portunus trituberculatus, and further deacetylated by alkaline boiling to make chitosan. The physical properties of chitosan solution and its film forming properties were examined. The functional characteristics of chitosan film were compared to those of cellophane, polyvinyl chloride (PVC) and polyethylene (PE) films. The proximate chemical composition of chitin obtained from crab residue was 6.95% nitrogen, 0.3% crude ash and 4.57% moisture and the product yield was 12.8% based on a dry material basis. The degree of deacetylation of chitosan was $79{\sim}92%$ and $70{\sim}86%$ as determined by IR spectroscopy, and $70{\sim}86%$ as determined by colloid titration method each respectively. The chitosan at 1% acetic acid solution showed distinct pseudoplastic flow behavior. The flow behavior index and consistency index were 0.8886, 0.2084 $MPa{\cdot}s^n$ for 0.4% solution and 0.8498, 0.6190 $MPa{\cdot}s^n$ for 0.8% solution, respectively. The chitosan film had the highest tensile strength $(888 kg/cm^2)$ and water permeability $(100\;g/m^2{\cdot}24\;hrs)$ among the tested films, but relatively low elongation property (49%). It showed the similar tear strength (90kg/cm) and light permeability (87.7%) to other films tested in spite of the relatively high haze value (12.5%). As the thickness of chitosan film increased from 0.025 to 0.050 mm, the tensile strength of film decreased distictively, and the degree of elongation, tear strength, and water permeability of film also decreased slightly. Whereas the light permeability of film did not change and the haziness of film slightly increased by the increase of film thickness.

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Nutritive Evaluation of Poultry Waste and Sudex Grass Silage for Sheep

  • Chaudhry, S.M.;Naseer, Z.;Alkraidees, M.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.1
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    • pp.79-85
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    • 1997
  • Cage layer waste and sudex grass were ensiled in the proportions of 0:100, 30:70, 40:60 and 50:50 wet basis, respectively. The influence of ensiling cage layer waste on Salmonellae, Shigella, Proteus, and total number of colony forming units (CFU) was investigated. The nutritive value of the silages was evaluated in a digestion trial. The experiment was conducted with 24 wethers allotted to four silages. Initial samples of cage layer waste showed $0.11{\times}10^6$ CFU and salmonellae, Shigella and Proteus were present. Ensiling was effective in complete elimination of all the pathogens. Dry matter, crude protein and ash contents were increased linearly (p < .01) with the increase of cage layer waste in the silages ($358g{\cdot}kg^{-1}$ to $484g{\cdot}kg^{-1}$; $76.3g{\cdot}kg^{-1}$ to $183.2g{\cdot}kg^{-1}$ and $38.5g{\cdot}kg^{-1}$ to $169.4g{\cdot}kg^{-1}$; DM basis, respectively). Water solubles carbohydrate values for silages were 38.3, 22.5, 20.1 and $20.0g{\cdot}kg^{-1}$ DM basis, respectively. Ensiling decreased the pH values for all the silages and the decrease was higher for sudex grass ensiled alone than grass ensiled with cage layer waste. Lactic acid concentration in silages increased linearly (p < .01) with the increase of cage layer waste. The apparent digestibilities of DM, OM and CP for the animals fed sudex grass silage alone were 496.0, 516.1, $496.7g{\cdot}kg^{-1}$ DM basis respectively. However, digestibilities of all the components were higher (p < 0.01) for the animals fed silages containing cage layer waste. Among waste containing diets, the digestibilities of all the components showed quadratic affect (p < 0.01), increased for silages containing 30 and 40% cage layer waste and decreased when the level of waste increased from 40 to 50%. The results indicated that cage layer waste can be used upto 40% in ruminant diet as a source of N without any adverse effect on the health of animals. Ensiling appeared to be feasible and effective method for eliminating the pathogen present in cage layer waste.

Growth Characteristics of Purple Nutsedge(Cyperus rotundus L.) and Establishment of Its Effective Control Method (향부자(Cyperus rotundus L.)의 생육특성 및 방제법에 관한 연구)

  • Kim, Kyoung-Im;Kim, Kil-Ung;Shin, Dong-Hyun;Lee, In-Jung
    • Korean Journal of Weed Science
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    • v.18 no.2
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    • pp.136-145
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    • 1998
  • This study was conducted to determine the growth characteristics, and the effect of plant growth regulators on the sprouting and growth of purple nutsedge(Cyperus rotundus L.) in order to establish effective control system in lawn ground. The flowering of purple nutsedge was initiated 30 days after transplanting regardless of the transplanting time. Low temperature less than $10^{\circ}C$ after flowering was required for tuber formation, showing that the tuberization was related to air temperature. Shoot number and dry weight of underground portion of purple nutsedge was slightly affected by plant growth regulators such as benzylamino purine, abscisic acid, brassinosteroid and jasmonate. Imazaquin applied at 1, 2 and 3 weeks after transplanting induced multi-shooting and inhibited shoot growth indicating that the herbicide played a role as plant growth regulator at a concentration of 30 and 60g ai/10a. The greatest inhibition of purple nutsedge was obtained by pyrazosulfuron-ethyl as applied 1 weeks after transplanting, showing almost 100% control of purple nutsedge. Tuber of purple nutsedge composed of 61.83% of moisture, 31.60% of carbohydrates, 4.03% of crude protein, 1.57% of crude fat and 0.97% of crude ash.

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Evaluation on Feed-Nutritional Change of Food Waste According to Different Processing Methods and Trouble-shooting Strategy (음식물쓰레기의 가공처리방법별 사료영양소 함량 변화 평가 및 문제점 개선 방안)

  • Jee, K.S.;Baik, Y.H.;Kwak, W.S.
    • Journal of Animal Science and Technology
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    • v.47 no.4
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    • pp.513-524
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    • 2005
  • This study was conducted to introduce recycling procedures of food waste(FW) as feed according to the dehydration, semi-dehydration fermentation and liquid fermentation methods through the on-site survey of companies related, to trace physico-chemical components and nutritional losses depending upon the processing stage for each method and finally to suggest more desirable methodology for the efficient utilization of FW as animal feed. For the dehydration method, dewatering of FW alone reduced(P<0.05) moisture(approximately 10%) and ether extract contents and increased(P<0.05) fiber contents. Dewatering and subsequent dehydration of FW decreased(P<0.05) contents of ether extract, limiting amino acids such as lysine, methionine and histidine, pepsin digestibility of protein by half, and NaCl content by 40%, increased(P<0.05) contents of fiber, crude ash, Ca and P, and did not alter(P>0.05) pH. The semi-dehydration fermentation method of FW did not affect(P>0.05) the chemical components, pepsin digestibility of protein, pH and NaCl content. For the liquid fermentation method, pasteurization and fermentation of FW decreased(P<0.05) contents of dry matter, ether extract, crude fiber, lysine and NaCl; however, it did not affect(P>0.05) other chemical components, pepsin digestibility of protein and pH. Among the processing methods, nutrient losses were highest for the dehydration method(25% of metabolizable energy loss, 12% of organic matter loss) and little for the semi-dehydration and liquid fermentation methods. The on-site survey of companies related revealed that the existence of foreign materials in FW products were problematic for all the three companies surveyed, thus it was necessary to develop a more efficient screener. Before feeding FW-containing diets to pigs, high quality of protein and energy feedstuffs needed to be fortified for the dehydration method. For the semi-dehydration fermentation method, the scientific diet formulation technology was required at the initial mixing stage. For the liquid fermentation method, possibly most energetic and proteinaceous feeds needed to be supplemented for the normal animal growth.

Study on the Contents of Trace Elements in Foods (on the Trace Element Contents of Shellfish in Korean coastal Water) (식품중의 미량금속에 관한 연구조사 (연안 견류중의 중금속 함유량에 관하여))

  • 백덕우;권우창;원경풍;김준한;김오한;소유섭;김영주;박건상;성덕화
    • Journal of Food Hygiene and Safety
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    • v.3 no.1
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    • pp.7-18
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    • 1988
  • In 1987, the level of heavy metals were determined ina total of 200 samples of 9 species of shellfish of Korea. The samples were collected at the fish. markets by 10 Public Institute of Health. The samples were whelk (Buccinum striatiBBimum), oyster (Crassostrea gigas), ark shell(Tegillarca granesa), shartnecked clam (Venerupis semidecussta), hard clam (Meretrix lusoria), top shell (Turbo cornutus), abalone (Haliotis gigantea), ark shell (Scapharea broughtonii), sea-mussel (Mytilus conuscus gould), respectively. The levels of total mercury, lead, cadmium, arsenic, copper, zinc and manganese were determined. The total mercury levels were determined by mercury analyzer using the combustion gold amalgamation method. The arsenic level were determined by spectrophotometry using colorimetric sil ver diethyldithiocarbamate method after dry ash dige8tion of the samples with magnesium oxide and magnesium nitrate. The levels of other metals were determined by inductively coupled pluma spectrophotometry after wet digestion of the samples with nitric acid and su1furic acid. The results were summerized as follows; 1. The overallranges and mean(ppm) were; Hg, ND-O.221 (0.036); Pb, 0.05-1.51 (0.37); Cd, 0.02-1.86 (0.61); As, 0.5-3.97 (1.22); Cu, 0.14-54.16 (4.93); Zn, 7.40-207.17 (30.09); Mn, 0.13-s.72 (3.40). 2. The levels of all 6 metals were found to be below the maximum permissible Iimits set by the Japan lor mercury, the Netherland for lead the Hong Kong for cadmium. The Finland for Arsenic no statutory Iimits for Zn and Mn in shellfish in any countries. 3. The results show that all the 9 species of shellfish studied, none have accumulated levels dangerous enough to pose a health problem.roblem.

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Evaluation of Moisture and Feed Values for Winter Annual Forage Crops Using Near Infrared Reflectance Spectroscopy (근적외선분광법을 이용한 동계사료작물 풀 사료의 수분함량 및 사료가치 평가)

  • Kim, Ji Hea;Lee, Ki Won;Oh, Mirae;Choi, Ki Choon;Yang, Seung Hak;Kim, Won Ho;Park, Hyung Soo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.2
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    • pp.114-120
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    • 2019
  • This study was carried out to explore the accuracy of near infrared spectroscopy(NIRS) for the prediction of moisture content and chemical parameters on winter annual forage crops. A population of 2454 winter annual forages representing a wide range in chemical parameters was used in this study. Samples of forage were scanned at 1nm intervals over the wavelength range 680-2500nm and the optical data was recorded as log 1/Reflectance(log 1/R), which scanned in intact fresh condition. The spectral data were regressed against a range of chemical parameters using partial least squares(PLS) multivariate analysis in conjunction with spectral math treatments to reduced the effect of extraneous noise. The optimum calibrations were selected based on the highest coefficients of determination in cross validation($R^2$) and the lowest standard error of cross-validation(SECV). The results of this study showed that NIRS calibration model to predict the moisture contents and chemical parameters had very high degree of accuracy except for barely. The $R^2$ and SECV for integrated winter annual forages calibration were 0.99(SECV 1.59%) for moisture, 0.89(SECV 1.15%) for acid detergent fiber, 0.86(SECV 1.43%) for neutral detergent fiber, 0.93(SECV 0.61%) for crude protein, 0.90(SECV 0.45%) for crude ash, and 0.82(SECV 3.76%) for relative feed value on a dry matter(%), respectively. Results of this experiment showed the possibility of NIRS method to predict the moisture and chemical composition of winter annual forage for routine analysis method to evaluate the feed value.

Effect of Pre-wilting on the Forage Quality of Organic Sorghum$\times$Sudangrass Silage (예건이 수수$\times$수단그라스 교잡종 유기 사일리지의 품질에 미치는 영향)

  • Lim, Hyun-Jin;Kim, Jong-Duk;Lee, Hyun-Jin;Jeon, Kyeong-Hup;Yang, Ka-Young;Kwon, Chan-Ho;Yoon, Sei-Hyung
    • Korean Journal of Organic Agriculture
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    • v.17 no.4
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    • pp.519-527
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    • 2009
  • The main nutritional problem of sorghum$\times$sudangrass hybrid silage is low quality and dry matter (DM) contents. This experiment was conducted to evaluate whether field pre-wilting treatment of sorghum$\times$sudangrass hybrid silage increases DM content and forage quality of the silage. The experiment was a randomized complete block design with three replications. The treatments were consisted of five different wilting days: 0 day (direct cut silage), 0.5 day, 1 day, 2 days and 3 days. Sorghum$\times$sudangrass hybrid silage with pre-wilting showed low 4.00 pH values, however direct cut silage (control) showed a 4.30 value because of its high moisture content. The DM content of sorghum$\times$sudangrass hybrid silage with pre-wilting was high above 25.1% after 1 days, however that of direct cut silage had 17.6%. And the effluent of wilted silage was decreased with prolonged wilting period, but that of direct cut silage produced effluent of 183 mL/kg. Crude protein and ether extract with wilted silage were decreased, however crude ash was increased with prolonged wilting period. Silage with pre-wilting had higher acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents than control silages, while its non-fiber carbohydrate (NFC) showed the opposite results. Lactic acid and total organic acid were increased with prolonged wilting period. For the palatability of dairy goat, silage with 2 days wilting was highest among silages. The experiment results indicate that wilted sorghum$\times$sudangrass hybrid silage could be recommended as effective method for reducing effluent, increasing pH and forage quality more than direct cut silage. Optimum pre-wilting day of sorghum$\times$sudangrass hybrid silage may be 1 days.

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