• Asian-Australasian Journal of Animal Sciences
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• 제25권2호
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• pp.240-247
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• 2012

Dietary supplementation with conventional linted cottonseed hulls (LCSH) is a common practice in livestock production all over the world. However, supplementation with mechanically delinted cottonseed hulls (DCSH) and cottonseed linter residue (CLR) is uncommon. Cottonseed by-products, including LCSH, DCSH and CLR, were assessed by chemical analysis, an in situ nylon bag technique, an in vitro cumulative gas production technique and in vitro enzyme procedure. The crude protein (CP) content of CLR (302 g/kg dry matter (DM)) was approximately 3 times that of LCSH and 5 times that of DCSH. The crude fat content was approximately 3 times higher in CLR (269 g/kg DM) than in LCSH and 4 times higher than in DCSH. Neutral detergent fibre (311 g/kg DM) and acid detergent fibre (243 g/kg DM) contents of CLR were less than half those of DCSH or LCSH. Metabolisable energy, estimated by in vitro gas production and chemical analyses, ranked as follows: CLR (12.69 kJ/kg DM)>LCSH (7.32 kJ/kg DM)>DCSH (5.82 kJ/kg DM). The in situ degradation trial showed that the highest values of effective degradability of DM and CP were obtained for CLR (p<0.05). The in vitro disappearance of ruminal DM ranked as follows: CLR>LCSH>DCSH (p<0.05). The lowest digestibility was observed for DCSH with a two-step in vitro digestion procedure (p<0.05). The potential gas production in the batch cultures did not differ for any of the three cottonseed by-product feeds. The highest concentration of total volatile fatty acids was observed in CLR after a 72 h incubation (p<0.05). The molar portions of methane were similar between all three treatments, with an average gas production of 22% (molar). The CLR contained a higher level of CP than did LCSH and DCSH, and CLR fermentation produced more propionate. The DCSH and LCSH had more NDF and ADF, which fermented into greater amounts of acetate.

• Asian-Australasian Journal of Animal Sciences
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• 제25권7호
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• pp.971-979
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• 2012

Four, lactating dairy crossbreds ($50%{\times}50%$ Holstein Friesian${\times}$Native Zebu cattle) were randomly assigned according to a $2{\times}2$ factorial arrangement (two protein levels and two levels of mangosteen peel pellets (Mago-pel)) in a $4{\times}4$ Latin square design to receive four dietary treatments. All cows received concentrate at a proportion of 1 kg concentrate per 2 kg of milk yield, and urea-treated 5% rice straw (UTRS) was given ad libitum. It was found that total dry matter intakes, nutrient digestibility, ruminal pH and $NH_3$-N concentrations were not affected (p>0.05) by treatments. Concentrations of ruminal pH and $NH_3$-N were not affected by dietary treatments although the concentration of BUN varied significantly (p<0.05) between protein levels (p<0.05). The populations of rumen bacteria and fungal zoospores did not differ among treatments (p>0.05); however, the population of protozoa was decreased (p<0.05) when cows received Mago-pel supplementation. The composition of the population of bacteria, identified by real-time PCR technique, including total bacteria, methanogens, Fibrobacter succinogenes and Ruminococcus albus was similar (p>0.05) among dietary treatments (p>0.05); however, copy numbers of Ruminococcus flavefaciens was increased when protein level increased (p<0.05). Microbial protein synthesis, in terms of both quantity and efficiency, was enriched by Mago-pel supplementation. Milk yield was greatest in cows fed UTRS based diets with concentrate containing protein at 16% CP with Mago-pel, but were lowest without Mago-pel (p<0.05). In addition, protein level and supplementation of Mago-pel did not affect (p>0.05) milk composition except solids-not-fat which was higher in cows fed the diet with 19% CP. Therefore, feeding a concentrate containing 16% CP together with 300 g/hd/d Mago-pel supplementation results in changes in rumen fermentation and microbial population and improvements in milk production in lactating dairy crossbreds fed on UTRS.

• 한국잡초학회지
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• 제5권2호
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• pp.131-136
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• 1985

수도(水稻)와 일년생(一年生) 광엽잡초(廣葉雜草)인 물달개비(Monochoria vaginalis Presl.)와 경합(競合)이 수도(水稻)의 생육(生育) 및 수량(收量)에 미치는 영향(影響)과 제초제(除草劑)에 대(對)한 물달개비의 반응(反應)을 조사(調査)하여 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. 수도(水稻)의 총건물중(總乾物重)은 무처리(無處理)에 비(比)해 물달개비의 밀도(密度)가 1본(本)에서 3본(本)으로 증가(增加)할수록 16%, 33%, 37%씩 각각(各各) 감소(減少)하였다. 2. 총건물중(總乾物重)으로 본 광합성(光合成) 효율(效率)은 경합비율(競合比率)이 높아짐에 따라 현저(顯著)히 떨어졌으며 상대적(相對的)으로 경합지수(競合指數)의 증가(增加)로 전체적(全體的)인 건물중(乾物重) 감소(減少)를 가져왔다. 3. 수도(水稻)와 물달개비의 경합(競合)으로 인(因)한 수량(收量)의 감소(減少)는 수수(穗數)와 수당(穗當) 영화수감소(領花數減少)가 가장 많은 영향(影響)을 미친 것으로 나타났다. 4. 제초제(除草劑)의 종류(種類)에 따라 물달개비의 초장억제율(草長抑制率)이 상이(相異)하나 기존(旣存) 제초제(除草劑)이 butachlor와 thiobencarb에 못지않는 유망(有望)에 신(新) 제초제(除草劑)가 많이 있음을 입증(立證)하였다.

• 농업과학연구
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• 제32권2호
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• pp.197-203
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• 2005

본 시험은 활성탄 및 목탄의 첨가가 사료의 소화율, 반추위내 pH 발효성상에 미치는 영향을 조사하기 위하여 실시하였다. 한국재래산양에 대한 급여 실험을 실시하였는데, 활성탄과 목탄의 첨가수준은 1.0% 수준이었으며, 조사료/농후사료 비율은 오차드그래스 건초와 농후사료를 2:8로 하였다. 시험에 공시된 동물은 대사케이지에 수용하여 매일 09시에 사료를 급여하였다. 시험 결과를 요약하면 건물 소화율은 활성탄 및 목탄 첨가구가 무첨가구에 비하여 유의한 결과는 아니었지만 높은 경향을 보였다. 그리고 단백질의 소화율은 활성탄 및 목탄 첨가구가 무첨가구에 비하여 유의하게 높은 결과(p<0.05)를 나타내었다. 그러나 조지방, NDF, ADF 및 hemicellulose의 소화율은 유의한 증가를 나타내지 않았다. 활성탄구와 목탄구 사이에도 유의한 차이를 나타내지 않았다. pH와 반추위내 암모니아의 농도는 활성탄구와 목탄구가 무첨가구보다 다소 높은 경향을 보였으며, 총VFA 농도는 무첨가구에서 유의하게 높게 나타났다. 그리고 $C_2/C_3$ 비율을 보면 활성탄구는 무처리구에 비하여 유의하게 낮은 결과(p<0.05)를 나타내었고, 목탄구도 무처리구보다는 낮은 결과였으나 유의성은 인정되지 않았다. 위 결과에서 보듯이 활성탄은 가축의 생산성 향상에 유리한 조건을 제공하는 경향이 있다고 보여진다. 그러나 이미 보고된 바와 같이 시험 조건에 따라 상반되는 결과를 보이기도 한다. 이것이 외부 환경요인이 사료처리 영향을 압도하는 것인지, 또는 어느 제한된 조건하에서만 활성탄의 영향이 발현되는가에 대하여 추가연구가 요구된다고 하겠다. 그리고 여기에 대한 경제성 여부도 검토되어져야 할 것으로 사료된다.

• 한국환경과학회지
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• 제15권5호
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• pp.405-415
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• 2006

Sixteen soil samples around six areas (residental area, traffic area, power plant area, incineration area and factory area) where the stationary and mobile sources of polycyclic aromatic hydrocarbons (PAHs) are estimated to be emitted in Jeju City, were collected during Feburuary to March, 2004, and analyzed for 16 PAHs recommended by US EPA as primary pollutants to investigate their distribution characteristics. The concentrations of total PAHs (t-PAHs) and total carcinogenic PAHs $(t-PAH_{CARC})$ in soils of Jeju City were in the range of $21.7\sim264.2ng/g$ on a dry weight basis with a mean value of 87.2 ng/g and $6.3\sim118.0ng/g$ with a mean value of 33.4 ng/g, respectively. The concentrations of t-PAHs were low in comparison with those in soils of other domestic and foreign countries. The mean concentrations of t-PAHs and $(t-PAH_{CARC})$ with area decreased in the following sequences: traffic area> incineration area > factory area > power generation area > harbor area enli residental area. The correlation between t-PAHs and $(t-PAH_{CARC})$ were very high $(\gamma^2=0.9701)$, indicating that $(t-PAH_{CARC})$ concentration increases in proportion with t-PAHs. Comparing the distribution ratio of ring PAHs with area among 16 PAHs, it decreased in the order of 4-ring > 5-ring > 6-ring > 3-ring > 2-ring in all the areas except for harbor area. whitens for harbor area it was similar among 3-, 4- and 5-ring with high value. Low and no correlations between t-PAHs and soil compositions (organic matter content and particle size distribution) were observed, which is considered to be caused by the complex factors, such as the loading and characteristics of PAHs and diverse soil environment change, etc. From the examination of the three PAH origin indices, such as LMW/HMW (low molecular weight $2\sim3$ ring PAHs over high molecular weight $4\sim6$ ring PAHs), phenanthrene/anthracene ratio and fluoranthene/pyrene ratio, it can be concluded that the soil PAH contaminations were ascribed to strong pyrogenic origin in ail areas except for harbor area and to both pyrogenic and petrogenic origins.

• Asian-Australasian Journal of Animal Sciences
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• 제16권12호
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• pp.1738-1742
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• 2003

The whole plant of crop maize was chopped and ensiled in double-layered polyethylene bags to determine the influence of residual sugar on the fermentation of lactic acid and aerobic stability by L. buchneri in whole crop maize silage made in airstress condition. There were a total of six treatments used in this experiment as follow: added 25 g de-mineralised water per kg chopped maize serving as control (con), 37.5 g glucose solution containing 12.5 g glucose ($g_1$), 75 g glucose solution containing 25 g glucose ($g_2$), 25 g, L,.buchneri suspension intended for $10^6$ cfu $g^{-1}$ (L.b.), $g_1$+L.b. and $g_2$+L.b. All silos were opened at day 91 after ensiling for measuring the pH values, microbiological enumeration, fermentative products and aerobic stability. The dry matter loss increased significantly (p<0.01) due to inclusion of sugar or L. buchneri. The lower lactic acid concentrations were observed (p<0.01) in silages inoculated with L. buchneri only or in combination with sugar addition than the correspondent uninoculated silages. Compared with control silage, ethanol production was about 3 or 6-fold higher due to addition 12.5 or 25 g glucose per kg chopped maize at ensiling. The silages added with sugar contained less acetic acid concentration (p<0.01) than control, but silages inoculated with L. buchneri showed the contrary effects (p<0.01) at different sugar levels. No butyric acid was found in uninoculated silages, silages inoculated with L. buchneri. producted more propionic acid, 1-propanol and butyric acid. Lactic acid bacteria counts increased markedly (p<0.01) due to inoculation with L. buchneri, whereas it was reduced (p<0.01) by added sugar. No significant difference was observed in count of yeast, but inoculation with L. buchneri shows a decreasing trend. Mould count in all silages was less than 2 (log cfu $g^{-1}$). The added sugar had negative effects on aerobic stability of maize silage made under air-stress conditions, whereas inoculation with L. buchneri improves (p<0.01) the aerobic stability.

• 한국작물학회지
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• 제39권4호
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• pp.397-404
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• 1994

경북 영천군 독농가가 재배한 정식 4년차인 의성종과 밀양종(P. lactiflora Pall.에 속하는 지방종)작약을 공시재료로 하여 생육시기별로 paeoniflorin 및 내용성분을 조사하고 수확기별로 약근수량을 조사한 결과를 요약해 보면 1. 개화기인 5월에 저장근 건물비율은 약 23%로 가장 낮았고, 수축율은 47%로 가장 높아 서로 상반된 양상을 나타내었으며, 6월 이후는 이들 특성의 변화가 없어 안정적이었다. 2. 전 생육기간 중 내용성분의 변화가 가장 큰 시기는 개화기인 5월이었으며, 약근에 함유된 paeoniflorin, 조단백, 조섬유 및 조회분은 공통적으로 개화기인 5월에 함량이 일시적으로 증가하였고, 총당 및 조지방은 변화가 적었다. 3. Paeoniflorin은 5월 시기에 6.37%로 가장 높았으며 6월 이후 변화없이 안정된 경향을 보였고, 의성종과 밀양종의 품종간 차이는 뚜렷하여 밀양종이 약 2% 높았다. 4. 전 생육기간 중 내용성분의 변화가 심하였던 전기(2∼5월)와 안정적인 후기(6∼10월)의 특성간 상관관계 중 전·후기 모두 정의 유의상관을 가진 것은 전분과 건물중, 조섬유와 paeoniflorin이며 부의 유의상관을 가진 특성은 전분과 조섬유, 전분과 paeoniflorin, 전분과 건물중 이었다. 5. 경장, 주당경수, 건전경비율은 이병으로 인한 고사로 8월보다 10월 수확기에서 현저히 낮았으며, 약근수와 길이도 근부현상이 심하여 10월 수확기에서 감소되었다. 6. 4년생 작약의 수확적기는 8∼9월이 적당하며 지상부 고사가 심하면 수확기를 앞당기는 것이 수량 및 품질면에서 더욱 안정적이다.

• 한국환경농학회지
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• 제19권2호
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• pp.166-170
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• 2000

본 연구는 우리 나라 콩 장려품종인 단원콩, 황금콩, 광안콩 등 3가지 품종을 대상으로 100mM NaCl 처리에 따른 30일 묘의 생육특성 및 광합성 반응을 조사하여 다음과 같은 결과를 얻었기에 보고하는 바이다. 주경장과 주경절수는 NaCl 처리로 감소하였으며, 엽면적 및 건물중 역시 크게 감소하였다. 품종간 차이는 그다지 크지 않았지만 황금콩이 다른 두품종보다 감소정도가 적은 것으로 나타났다. 잎, 줄기, 뿌리로 구분한 건물중 변화는 줄기의 건물중이 가장 크게 감소하였으며 다음이 잎, 뿌리 순으로 NaCl에 영향을 받았다. 비엽면적(SLA), 지상부와 지하부 비율(Shoot/Root), 잎과 지하부의 비율(Leaf/Root)은 황금콩이 처리간에 차이가 없었으나 나머지 두 품종은 NaCl 처리로 감소하였으며, 옆위별 잎 두께는 무처리는 옆위간에 큰 차이를 보이지 않았지만 100mM MaCl 처리는 일정한 경향을 보이지 않았다. 제 1엽과 2엽의 엽록소 함량의 경시적 변화는 무처리는 처리 후 15일째에 급격히 증가하였지만 NaCl 처리는 점차 감소하는 경향을 보였다. 제 2엽의 광합성 속도는 경시적으로 일정한 경향을 보였지만 NaCl 처리로 광합성 속다가 감소하였으며 처리 후 20일째에 가장 많이 감소하였다. 광합성 속도와 기공전도도 및 증산속도와의 관계는 유의한 상관이 인정되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.661-666
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• 2005

The effects of pre-partum feeding management in terms of birth weight, growth, metabolic profile and immunity of calves were studied using 24 crossbred (Bos taurus${\times}$Bos indicus) cows, divided into three equal groups. The dietary treatments included feeding of either 3.0 kg concentrate/head/d throughout the 60 d pre-partum (T$_1$), or 3.0 kg concentrate during 60-22 d pre-partum and thereafter at an increased allowance at 0.25 kg/d during the next 21 d till it reached 1% of live weight (T$_2$). The third group of cows was fed similar to T$_2$, except that the concentrate feeding during 60-22 d pre-partum was reduced to 2.0 kg (T$_3$). All the groups had access to ad libitum green fodder throughout. The results revealed that the mean daily dry matter (DM) intake by the cows was similar (p>0.05) among the three groups during the 60 days of the pre-partum but T2 animals tended to gain more live weight (41.25 kg) than T$_1$ (38.12 kg) and T$_3$ (36.25 kg). The body condition score of the cows did not change appreciably over the experimental period. The mean birth weight of the calves was 24.00${\pm}$1.10, 24.63${\pm}$1.17 and 23.25${\pm}$1.19 kg for the three groups, respectively, with the corresponding average daily gain of 154.2, 155.0 and 169.7 g during the subsequent 60 days; both these parameters did not vary significantly ascribable to prepartum feeding regimens of their dams. The total immunoglobulin (Ig) concentration in the colostrum was 6.31${\pm}$0.34, 5.80${\pm}$0.21 and 6.13${\pm}$0.30 g/dl for the three groups, respectively, showing no influence of dietary treatments. The mean serum Ig levels (T$_1$ 2.10${\pm}$0.09, T$_2$ 2.05${\pm}$0.09 and T$_3$ 2.10${\pm}$0.12 g/dl) of calves at 5 d of age were similar among the dietary groups as was the case with various serum biochemical constituents. It is concluded that the variations in pre-partum dietary management elicited no significant influence on the calf performance including the immune status.

• Asian-Australasian Journal of Animal Sciences
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• 제29권9호
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• pp.1280-1286
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• 2016

This study was aimed to evaluate the in vitro effects of medicinal herb extracts (MHEs) on ruminal fermentation characteristics and the inhibition of protozoa to reduce methane production in the rumen. A fistulated Hanwoo was used as a donor of rumen fluid. The MHEs (T1, Veratrum patulum; T2, Iris ensata var. spontanea; T3, Arisaema ringens; T4, Carduus crispus; T5, Pueraria thunbergiana) were added to the in vitro fermentation bottles containing the rumen fluid and medium. Total volatile fatty acid (tVFA), total gas production, gas profiles, and the ruminal microbe communities were measured. The tVFA concentration was increased or decreased as compared to the control, and there was a significant (p<0.05) difference after 24 h incubation. pH and ruminal disappearance of dry matter did not show significant difference. As the in vitro ruminal fermentation progressed, total gas production in added MHEs was increased, while the methane production was decreased compared to the control. In particular, Arisaema ringens extract led to decrease methane production by more than 43%. In addition, the result of real-time polymerase chain reaction indicted that the protozoa population in all added MHEs decreased more than that of the control. In conclusion, the results of this study indicated that MHEs could have properties that decrease ruminal methanogenesis by inhibiting protozoa species and might be promising feed additives for ruminants.