• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.396-403
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• 2019

This study was conducted to investigate the growth of grafted cucumber seedlings in biodegradable paper pot trays influenced by seedling age, nutrient management before transplanting, and night temperature after transplanting. Grafted cucumber seedlings in paper pot trays were supplied with different nutrient solution concentrations of 0.5 x full strength (S) (EC $0.8dS{\cdot}m^{-1}$), 1.0S(EC $1.6dS{\cdot}m^{-1}$), 2.0S(EC $3.2dS{\cdot}m^{-1}$) two times a week until transplanting. 26, 33, 40, and 47 day-old cucumber grafted seedlings were transplanted and grown at three levels of night temperature (10, 15, and $25^{\circ}C$) during ten days. Increasing nutrient solution concentration enhanced the shoot length, number of leaves, leaf area, dry weight, and relative growth rate of seedlings. With increasing seedling age, the differences in growth were greater among nutrient treatments. The dry matter percentage increased with the seedling age, but was lower with higher nutrient concentration. The specific leaf area showed the opposite results. In cucumbers transplanted at 26- or 33-day seedling ages, night temperature did not affect the growth at ten days after transplanting. However, the growth of 40 or 47 day-old seedling decreased at $10^{\circ}C$. Compared with $25^{\circ}C$, the dry weight of cucumbers transplanted at 40- or 47-day seedling ages was depressed by 58% or 71%, respectively, at $10^{\circ}C$. Accordingly, it was concluded that the optimum nutrient solution concentrations and seedling age for the production of grafted cucumber seedlings in biodegradable paper pot trays can be 1.0S and about 30 days, respectively, and night temperature should be maintained at the range of $15-25^{\circ}C$ for promoting the growth after transplanting.

• Korean Journal of Environment and Ecology
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• v.33 no.5
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• pp.605-619
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• 2019

The objective of this study was to analyze eutrophication characteristics, empirical model analysis, and variation of water quality according to monsoon intensity in Hapcheon Reservoir for 16 years from 2002 to 2017. Long-term annual water quality analysis showed that Hapcheon Reservoir was in a meso-nutrition to eutrophic condition, and the eutrophic state intensified after the summer monsoon. Annual rainfall volume (high vs. low rainfall) and the seasonal intensity in each year were the key factors that regulate the long-term water quality variation provided that there is no significant change of the point- and non-point source in the watershed. Dry years and wet years showed significant differences in the concentrations of TP, TN, BOD, and conductivity, indicating that precipitation had the most direct influence on nutrients and organic matter dynamics. Nutrient indicators (TP, TN), organic pollution indicators (BOD, COD), total suspended solids, and chlorophyll-a (Chl-a), which was an estimator of primary productivity, had significant positive relations (p<0.05) with precipitation. The Chl-a concentration, which is an indicator of green algae, was highly correlated with TP, TN, and BOD, which differed from other lakes that showed the lower Chl-a concentration when nutrients increased excessively. Empirical model analysis of log-transformed TN, TP, and Chl-a indicated that the Chl-a concentration was linearly regulated by phosphorus concentration, but not by nitrogen concentration. Spatial regression analysis of the riverine, transition, and lacustrine zones of $log_{10}TN$, $log_{10}TP$, and $log_{10}CHL$ showed that TN and Chl-a had significant relations (p<0.005) while TN and Chl-a had p > 0.05, indicating that phosphorus had a key role in the algal growth. Moreover, the higher correlation of both $log_{10}TP$ and $log_{10}TN$ to $log_{10}CHL$ in the riverine zone than the lacustrine zone indicated that there was little impact of inorganic suspended solids on the light limitation in the riverine zone.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.12
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• pp.1736-1741
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• 2015

The objective of this study was to determine the effect of forage: concentrate ratio (F:C) on growth performance, ruminal fermentation and blood metabolites of housing-feeding yaks. Thirty-two Maiwa male yaks (initial body weight = $207.99{\pm}3.31kg$) were randomly assigned to four dietary treatments (8 yaks per treatment). Experimental diets were: A, B, C, D which contained 70:30, 60:40, 50:50 and 40:60 F:C ratios, respectively. Dry matter intake and average daily gain in yaks fed the C and D diets were greater (p<0.05) than yaks fed the A and B diets. No differences were found in ruminal $NH_3-N$, total volatile fatty acids, acetate, butyrate, valerate, and isovalerate concentrations. The propionate concentration was increased (p<0.05) in the C and D groups compared with the A and B diets. In contrast, the acetate to propionate ratio was decreased and was lowest (p<0.05) in the C group relative to the A and B diets, but was similar with the D group. For blood metabolites, no differences were found in serum concentrations of urea-N, albumin, triglyceride, cholesterol, low density lipoprotein, alanine aminotransferase, and aspartate aminotransferase (p>0.05) among treatments. Treatment C had a higher concentration of total protein and high density lipoprotein (p<0.05) than A and B groups. In addition, there was a trend that the globulin concentration of A group was lower than other treatments (p = 0.079). Results from this study suggest that increasing the level of concentrate from 30% to 50% exerted a positive effect on growth performance, rumen fermentation and blood metabolites in yaks.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.5
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• pp.732-741
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• 2020

Objective: The study was conducted to investigate the effects of Broussonetia papyrifera L. (B. papyrifera) silage on growth performance, serum biochemical parameters, meat quality, and meat amino acids and fatty acids compositions in beef cattle. Methods: Sixty-four male Angus beef cattle were assigned to 4 groups with 4 pens in each group and 4 beef cattle in each pen, and fed with the total mixed ration supplemented with 0%, 5%, 10%, or 15% B. papyrifera silage for 100 days (control group, 5% group, 10% group and 15% group) separately. Results: Beef cattle had significantly higher final body weight (BW) in 15% group, higher average daily gain (ADG) and dry matter intake (DMI) in 5% group, 10% group and 15% group, and higher feed conversion ratio (FCR) in 10% group and 15% group. Significantly higher blood superoxide dismutase (SOD) concentration was noted in 15% group, higher blood total antioxidant capacity (TAC) in 10% group and 15% group, lower 8-hydroxydeoxyguanosine (8-OHdG) and malondialdehyde (MDA) in 15% group. Meat had lower pH in 15% group, higher Commission International DeI'Eclairage (CIE) L^⋆ in 5% group, 10% group, and 15% group, and lower drip loss in 15% group. Greater concentration of meat polyunsaturated fatty acids (PUFA) was observed in 10% group and 15% group, and docosahexaenoic acid (DHA) in 15% group. Conclusion: Diet with 15% B. papyrifera silage could improve performance and increase final BW, ADG, DMI, and FCR, enhance the antioxidant functions by decreasing blood 8-OHdG and MDA and increasing blood SOD and TAC, improve the meat quality by lowing pH and drip loss and increasing CIE L^⋆, increase the meat PUFA and DHA concentration. Polyphenols and flavonoids might be the main components responsible for the antioxidant activity and anti-biohydrogenation in the B. papyrifera silage. And B. papyrifera silage could be used as a new feedstuff in beef cattle nutrition.

• Journal of Animal Science and Technology
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• v.47 no.3
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• pp.397-408
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• 2005

This study, consisting of three experiments, was conducted to determine the effects of feeding feather meal (FM), feather meal digest (FMD), L-cystine and methionine hydroxyl analogue (MHA) on taurine content of milk and milk production of Holstein dairy cows. In experiment 1, FM or FMD was supplemented at 0, 1, 3 and 5% of dry matter intake (DMI), respectively. Taurine concentration of 3% FM and 5% FMD treatment were increased by 14% and 220/0, respectively. The 5% FM treatment had a negative effect on milk yield and FM and FMD treatments had no significant or consistent effects on milk fat, protein, lactose, milk urea nitrogen (MUN) and somatic cell count (SCC). In experiment 2, Lcystine or MHA was supplemented at 0, 1, 3, and 5g or ml/d along with 5% FMD, respectively. Milk yield decreased at 3 and 5g or ml Lcystine or MHA supplementation along with 5% FMD. Fat and lactose in milk were not significantly affected by treatments. However, milk protein level increased significantly in the 5 ml HMA with 5% FMD treatment. SCC decreased significantly in 1ml MHA with 5% FMD supplemented treatment but increased in 5g Lcystine with 5% FMD and 5 ml MHA with 5% FMD treatments. Increase of milk taurine concentration of L'cystine with 5% FMD treatments was not significant but those of MHA with 5% FMD treatments were significantly higher than the control. The highest increase of milk taurine concentration was 65% shown in 1 ml MHA with 5% FMD treatment. In experiment 3, 5% FM, 5% FM+3% molasses or 5% FM+3% molasses+l ml MHA was supplemented to the based TMR diet. The molasses treatments (5% FM+3% molasses and 5% FM+3% molasses+l ml MHA) showed significantly higher milk taurine content than the 5% FM treatment. The molasses treatments significantly reduced MUN but increased SCC. It was concluded that FMD is more effective than FM in enriching taurine in milk. Maximum taurine enrichment (65%) in the milk was obtained by supplementation of 5% FMD/DM1+1 ml MHA/d/cow. Molasses supplementation to 5% FM diet increased milk taurine content. However, MHA supplementation in dairy cows increased ruminal escape, gastrointestinal absorption and response of serum methionine.

• Korean Journal of Medicinal Crop Science
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• v.19 no.2
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• pp.126-135
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• 2011

Various herbal medicinal extracts were examined for the development of cosmetic products with anti-oxidative and anti-wrinkle activity. First, total polyphenol contents and DPPH radical scavenging activities of herbal medicinal extracts were measured. Most herbal samples, except for DW extracts of Portulaca oleracea, Caesalpinia sappan, Taraxacum platycarpum, Carthamus tinctorius, and 70% EtOH extracts of Taraxacum platycarpum and Carthamus tinctorius, showed DPPH radical scavenging activity over 80% at a concentration of $400{\mu}g/m{\ell}$. SOD-like antioxidant activity of DW extracts of Syzygium aromaticum, DW extracts of Eriobtrya japonica and 70% EtOH extracts of Sophora japonica was measured as 40%, 35% and 80%, respectively at a dry matter concentration of $50{\mu}g/m{\ell}$. In elastase inhibition assay, DW extracts of Lycium chinense ($50{\mu}g/m{\ell}$) and 70% EtOH extracts of Areca catechu ($50{\mu}g/m{\ell}$) showed 50% and 40% of inhibition, respectively. At a concentration of $1.250{\mu}g/m{\ell}$, DW extracts of Lycium chinense and 70% EtOH extracts of Areca catechu showed 10% and 30% of collagenase inhibition, respectively. Skin and lotion samples were prepared using the two herbal extracts of high anti-wrinkle activity: Lycium chinense extract and Areca catechu extract. The storage stability of skin and lotion containing each of the selected herbal extracts was evaluated. pH and viscosity were used as stability indicators for the stability test under different storage temperatures and freeze-thaw cycle conditions. The skin and lotion containing each of DW extract of Lycium chinense and 70% EtOH extract of Areca catechu was showed high pH and viscosity stability. The skin and lotion containing DW extracts of Lycium chinense showed relatively higher stability than the skin and lotion containing 70% EtOH extract of Areca catechu, at cycle chamber and freeze-thaw conditions. In summary, these results indicated that cosmetics containing DW extract of Lycium chinense were relatively stable, and this herbal extract could be used as a stable functional cosmetic material.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.6
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• pp.852-862
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• 2003

An experiment was conducted to study the effect of ionophore enriched cold processed mineral block supplemented with urea molasses on microbial growth and rumen fermentation. Twelve adult male crossbred cattle were divided into four groups on body weight basis. Animals were given wheat straw as a basal diet. The animals of group I and II were supplemented with concentrate mixture and animals of group III and IV were supplemented with cold processed urea molasses mineral block (UMMB). Thirty mg monensin/day/animal were supplemented to the animals of group II and 35 ppm monensin were incorporated in the UMMB supplemented to the animals of group IV. Dry matter (DM) intake did not differ significantly among groups. Mean rumen pH was higher in UMMB fed animals. Total volatile fatty acids (TVFA) concentration (mmole/L strained rumen liquor (SRL) in group III (113.19) was significantly (p<0.05) higher than those of group I (105.83) and II (108.74) but similar to group IV (109.34). TVFA production (mole/day) was similar in all the groups. The molar proportion of acetate was significantly (p<0.01) higher in the group I (59.56) than those of group II (51.73) and IV (55.91) but similar to group III (57.12). The molar proportion of propionate was significantly (p<0.01) higher in the monensin treated groups i.e. group II (38.38) and IV (36.26) than those of group I (27.78) and III (33.06). Butyrate molar percent was significantly (p<0.01) higher in group I (12.65) than those of group II (10.19), group III (9.83) and IV (7.84). The reduction of acetate and butyrate was due to UMMB and monensin resulted in lower A:P ratio. Average bacterial pool and bacterial production rate did not differ significantly among groups. Total N concentration (mg/100 ml SRL) was significantly (p<0.01) higher in the group I (55.30) and III (57.70) as compared to the group II (47.97) and IV (47.59). Ammonia-N concentration (mg/100 ml SRL) of group III (34.99) was significantly (p<0.01) higher than that of the group I (25.76) which was again significantly (p<0.01) higher than that of the group II (20.79) and IV (19.83) indicating slower release of ammonia due to monensin in diet. Total bacterial, cellulolytic, proteolytic bacterial and fungal count at 4 h post feeding did not differ significantly (p<0.05) among treatment groups. However, methanogenic bacterial count was significantly (p<0.01) higher in the group I (11.80) compared to the group II (8.43) which was significantly (p<0.01) higher than that of the group III (4.70) and IV (2.90). Average protozoal population was affected by both treatments. Thus feeding of UMMB and monensin in diet affected the rumen fermentation pattern towards propionate production, slower release of ammonia and reduction in methanogenic bacteria in the rumen.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.12
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• pp.1674-1680
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• 2000

A study was conducted to compare the feeding value of urea treated and untreated mustard straw (MS) for sheep. Treated MS was prepared by adding urea-N at 1.84% and followed by packing in a pit silo for 21 days. Two groups of six empty Avikaline ewes were fed untreated (UTMS) and treated (TMS) mustard straw along with 200 g concentrate per head daily for 90 days. Untreated MS had 0.41% N and the urea treatment increased its N value to 1.58 %. The cell wall constituents were decreased in the TMS except for cellulose which remained unaffected. Dry matter intake of TMS was consistently higher than that of UTMS. Digestibility of DM, OM and fibre fractions of MS improved by the urea treatment. Ewes in both groups were in positive N balance while % N retention was lower in UTMS (26.30%) than in TMS (52.14%). The TMS fed group on average consumed 30.2 g DM, 2.9 g digestible crude protein and $0.2MJ\;DE\;per\;kg\;BW\;day^{-1}$ and maintained their weight whereas, the UTMS fed ewes lost weight. The VFA concentration in rumen liquor was higher in TMS than in UTMS. Total-N, ammonia-N and TCA-precipitable-N were also higher in TMS fed ewes. Blood glucose concentrations in the two groups were similar at initiation of the study. However the glucose concentration of UTMS fed group was significantly (p<0.01) lower than those fed UTMS at the termination of the study. Urea-N concentration was also higher in TMS fed group after 90 days of feeding period. It is concluded that urea treatment of MS improved N value of MS from 0.41% to 1.58% along with sizable improvement in nutritive value and in conjunction with 200 g concentrate, TMS can serve as maintenance ration for sheep. ($ME_{lakt}/ME_{m}=1.46$).

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.3
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• pp.358-365
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• 2013

A series of in vitro studies were carried out to determine i) the effects of enzyme and formaldehyde treatment on the degradation characteristics of carbohydrate and protein sources and on the synchronicity of these processes, and ii) the effects of synchronizing carbohydrate and protein supply on rumen fermentation and microbial protein synthesis (MPS) in in vitro experiments. Untreated corn (C) and enzyme-treated corn (EC) were combined with soy bean meal with (ES) and without (S) enzyme treatment or formaldehyde treatment (FS). Six experimental feeds (CS, CES, CFS, ECS, ECES and ECFS) with different synchrony indices were prepared. Highly synchronous diets had the greatest dry matter (DM) digestibility when untreated corn was used. However, the degree of synchronicity did not influence DM digestibility when EC was mixed with various soybean meals. At time points of 12 h and 24 h of incubation, EC-containing diets showed lower ammonia-N concentrations than those of C-containing diets, irrespective of the degree of synchronicity, indicating that more efficient utilization of ammonia-N for MPS was achieved by ruminal microorganisms when EC was offered as a carbohydrate source. Within C-containing treatments, the purine base concentration increased as the diets were more synchronized. This effect was not observed when EC was offered. There were significant effects on VFA concentration of both C and S treatments and their interactions. Similar to purine concentrations, total VFA production and individual VFA concentration in the groups containing EC as an energy source was higher than those of other groups (CS, CES and CFS). The results of the present study suggested that the availability of energy or the protein source are the most limiting factors for rumen fermentation and MPS, rather than the degree of synchronicity.

• Journal of Environmental Science International
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• v.22 no.7
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• pp.787-799
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• 2013

The aim of this study was to investigate the accumulation of metallic elements and the control effect of marine pollution caused by ocean dumping in the sediments at a waste disposal area in the Yellow Sea. In July 2009, concentrations of organic matter and metallic elements (Al, Fe, As, Cd, Cr, Co, Hg, Ni, Mn, Pb, and Zn) were measured in surface sediments at the site. The ignition loss (IL) in the surface sediments showed a mean value of 15.4%, about 1.5 times higher than the mean value of the sediments in the coastal areas of Korea. The chemical oxygen demand (COD) at some disposal sites exceeded 20 mg $O_2/g{\cdot}dry$, which signifies the initial concentration of marine sediment pollutants in Japan. The disposal sites contain higher concentrations of Cr, Cu and Zn than the sediments of bays and estuaries that might be contaminated. The magnitude of both metal enrichment factors (EF) and adverse biological effects suggest that pollution with Cr and Ni occurred due to the dumping of waste in the study area. In addition, the geoaccumulation index (Igeo) showed that the surface sediments were moderately contaminated. By the mid-2000s, when the amount of waste dumped at this site was the highest, the concentration of metallic elements was higher than ever recorded. On the other hand, in 2008-09, the need for environmental management was relatively low compare with the peak. As a result, the quality of marine sediment has been enhanced, considering the effect of waste reduction and natural dilution in the disposal area.