Yan Fen Li;Li Li Wang;Young Sang Yu;Xaysana Panyavong;Hak Jin Kim;Jong Geun Kim
Journal of The Korean Society of Grassland and Forage Science
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v.43
no.3
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pp.129-137
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2023
Corn silage is extensively utilized in ruminant feeding on a global scale, with substantial research efforts directed towards enhancing its nutritional worth and managing moisture content. The purpose of this study was to assess the impact of normal cutting height and elevated cutting height on whole-crop corn silage. Corn was harvested at heights of 15 cm and 45 cm above the ground, respectively, 45 days after heading. The harvested corn was cut into 2-3 cm lengths and packed into 20-liter plastic silos in triplicate. The results showed that dry matter (DM), crude protein (CP), water soluble carbohydrates (WSC), and in vitro dry matter digestibility (IVDMD) of C45 were significantly higher than those of the control, while the neutral detergent fiber (NDF) was significantly lower in C45 (p<0.05). The C15 had higher yields than C45 (p<0.05). There was no significant difference in the total digestible nutrients (TDN) yield of whole-crop corn silage. The increase in cutting height resulted in a larger change in moisture content and NDF per centimeter. After 60 days-ensiling, C45 showed significantly lower NH3-N concentrations. Moreover, C45 had significantly higher lactic acid concentration, lactic acid/acetic acid ratio, and lactic acid bacteria count compared to the control. Mold was not detected and the yeast count was less than 2 log10 cfu/g fresh matter in both control and C45. In summary, C45 improved the feeding value and fermentation quality of whole-crop corn silage at the expense of forage productivity.
Fadel Elseed, A.M.A.;Amin, A.E.;Khadiga,;Abdel Ati, A.;Sekine, J.;Hishinuma, M.;Hamana, K.
Asian-Australasian Journal of Animal Sciences
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v.15
no.6
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pp.844-850
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2002
The potential nutritive value was studied on leaves of seven fodder trees in Central Sudan during dry season at two distinct periods, the early dry and the late. The chemical composition, mineral concentration, in vitro organic matter digestibility (IVOMD), in situ OM or nitrogen degradability and estimated metabolizable energy showed a wide variation among fodder tree species and between different periods of the dry season. Crude protein (CP) ranged from 285 to 197 g/kg DM at early dry season, with a significant reduction in late dry season. Ziziphus spina-christi and Balanites aegyptiaca showed the least reduction in CP content. The NDF, ADF and lignin were about 200, 160 and 19 g/kg DM, respectively at the early period, and significantly increased at the late period of the dry season, except for lignin of Z. spina-christi. For mineral concentration, all fodder tree leaves were rich in calcium but poor in phosphorus. In situ OM degradability significantly decreased at the late period of dry season, but values remained as high as over 600 g/kg OM. At both periods, Z. spina-christi showed the highest value, while the lowest was recorded in Acacia seyal. The IVOMD showed a similar trend to those of in situ OM degradability, except for A. seyal. The nitrogen degradability was highest in B. aegyptiaca and lowest in Z. spina-christi at both periods. A significant and positive correlation had existed between CP and IVOMD or in situ OM degradability (r=0.68, p<0.05; r=0.77, p<0.05, respectively). Also, a significant but negative correlation was found between condensed tannins and nitrogen degradability (r=-0.87, p<0.01). Results demonstrated that Z. spina-christi potentially has a good nutritive value as dry season feed or supplement, while A. seyal tends to be less promising. A. nubica and B. aegyptiaca may be a useful source for degradable protein, even though it may have a limited supply of energy to animals. A. tortilis, A. mellifera and A. ehrenbergiana may have potential value for a supplementation of energy or protein, if they were harvested in the early dry season or in wet season as preserved feed. It is highly recommended to supplement with an appropriate amount of phosphorus when these fodder trees were used.
Surface sediments and seawater were sampled at Masan and Haengam Bays of Korea, to evaluate contamination by sewage-derived organic matter using fecal sterols, Six stream-water samples into Masan and Haengam Bays were also sampled. Total concentrations of eight sterols (coprostanol, epichloestanol, epicoprostanol, cholesterol, cholestanol, brassicasterol, stigmasterol and $\beta-sitosterol$) were in the range of $1,274\~4,768{\mu}g/g$ dry weight in suspended particulate from the stream-water, $292\~2,244{\mu}g/g$ dry weight in suspended particulate from the seawater and $4.5\~27.2{\mu}g/g$ dry weight in the sediments. Although sterol compositions in sediments, seawater and stream-water were different, cholesterol was the predominant sterol in all samples. The proportion and concentration of coprostanol, a sewage tracer, in stream-water was much higher than those in sediment and seawater. The sterol levels including coprostanol in the sediments and seawater were higher at inner basin than at the outer bay. Some molecular indices and multivariate statistical analysis were used to assess the origin of these sterols and sewage contamination in the study area. The sterol composition patterns in stream-water were mainly associated with contamination by sewage-derived organic matters and those of seawater were associated with the activities of marine-originated organisms. Sterol levels in the sediments were both from the sewage input through stream-water and the marine- originated organisms. This survey suggests that the main source/route of sewage-derived organic matters in Masan Bay is the input of stream-water into the bay.
The effect of concentrate mixtures with crude protein (CP) levels 10%, 13%, 16%, and 19% and diets with roughage to concentrate ratios 80:20, 60:40, 40:60, and 20:80 (w/w) were determined on dry matter (DM) and organic matter (OM) digestibility, and fermentation metabolites using an in vitro fermentation technique. In vitro fermented attributes were measured after 4, 24, and 48 h of incubation respectively. The digestibility of DM and OM, and total volatile fatty acid (VFA) increased whereas pH decreased with the increased amount of concentrate in the diet (p<0.001), however CP levels of concentrate did not have any influence on these attributes. Gas production reduced with increased CP levels, while it increased with increasing concentrate levels. Ammonia nitrogen ($NH_3$-N) concentration and microbial CP production increased significantly (p<0.05) by increasing CP levels and with increasing concentrate levels in diet as well, however, no significant difference was found between 16% and 19% CP levels. Therefore, 16% CP in concentrate and increasing proportion of concentrate up to 80% in diet all had improved digestibility of DM and organic matter, and higher microbial protein production, with improved fermentation characteristics.
Vranic, Marina;Knezevic, Mladen;Perculija, Goran;Bosnjak, Kresimir;Leto, Josip
Asian-Australasian Journal of Animal Sciences
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v.22
no.2
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pp.225-231
/
2009
The objective of this experiment was to study the effects of grass maturity at harvest on the nutritive value of grass silage (GS) in relation to voluntary intake, digestibility, nitrogen (N) utilization and in sacco dry matter (DM) degradability. Silage was cut from a sward dominated by orchardgrass (Dactylis glomerata L.) at the late-vegetative (early-cut), internode elongation (mediumcut) and flowering (late-cut) stages of growth. The DM yield at harvest was the lowest for early-cut silage (5.4 t/ha) and increased to 6.5 and 7.0 t/ha for the medium and late-cut silage respectively. As the crop matured, the crude protein (CP) concentration decreased significantly (p<0.05) and there was a marked increase in acid detergent fiber (ADF) concentration (p<0.001). The three different silages were offered to four 18-month old Charolais wether sheep to measure the voluntary intake, in vivo digestibility and N retention over four 21-day periods in an incomplete changeover design. Silage degradability characteristics were determined using four fistulated sheep to measure DM degradability over 3, 6, 12, 24, 48 and 72 h. There was a linear decrease in the voluntary intake of silage fresh matter, DM, organic matter (OM) and neutral detergent fiber (NDF), digestibility of DM, OM, NDF, ADF and CP, and digestibility of OM in DM (Dvalue) ($P_L<0.01$) as harvesting of grass was delayed. Nitrogen intake, N output in urine, N output in faeces and N balance also linearly decreased ($P_L<0.01$) with postponed harvesting of grass for silage. DM degradability and effective degradability (ED) significantly decreased with increasing maturity of grass at harvest. The results suggest that harvesting date has a significant influence on the nutritive value of GS in terms of intake, digestibility, N balance and in sacco degradability in the rumen. It was concluded that early harvest GS ensured higher intake, digestibility, N intake and DM degradability in comparison with the medium and the late cut GS as a result of improved rumen N efficiency and utilization probably due to a better balance of available energy and protein.
In this study, temperature, turbidity, suspended paniculate matter (SPM) distribution and mineral characteristics were investigated to explain spatial distribution of the turbid-water environment of Yongdam reservoir in July, 2005. Six stations were selected along a longitudinal axis of the reservoir and sampling was conducted in four depths of each station. Water temperature was showed the typical stratified structure by the effects of irradiance and inflow. Content of inorganic matter in suspended particles increased with the concentration of suspended particulate matter (SPM) due to the reduction of ash-free dry matter (AFDM). Turbidity ranged from 0.6 to 95.1 NTU and the maximum turbidity value of each station sharply increased toward downstream from upstream. The high turbidity layers were located at the depth between 12~16 m. Particle size ranged from 0.435 to $482.9{\mu}m$. day and silt-sized particles corresponded 91.9~98.9% and 1.1~8.0% in total numbers of SPM, respectively. Turbidity showed high correlations with clay (r=0.763, p<0.05) and silt content (r=0.870, p<0.05).Inorganic matter content (r=0.960, p<0.01) was more correlated with turbidity than organic matter (r=0.823, p<0.05). Mineral characterization using x-ray diffraction and electron probe microanalyzer demonstrated that the major minerals contained in the SPM were kaolinite, illite, vermiculite and smectite. As results of this study, surface water discharge as well as small size of the SPM were suggested as long-term interfering factors in settling down the turbid water in the reservoir.
Han, Sim-Hee;Kim, Du-Hyun;Ultra, Venecio U. Jr.;Lee, Jae-Cheon
Journal of Korean Society of Forest Science
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v.99
no.5
/
pp.711-719
/
2010
We investigated physiological responses and phytoextraction ability of Pinus thunbergii in cadmium contaminated soil as part of our efforts in identifying plant materials for the restoration and revegetation of forest soil contaminated by heavy metals. Thirty seedlings (ten per treatment) were assigned to three treatments (control, 0.3 and 0.6 mM $CdSO_4$ solution) at first year experiment. At second year, ten seedlings per treatment treated with Cd during the first year experiment were divided by two groups (no Cd-treated and consecutive Cd-treated group). At first experiment, photosynthetic pigment content, and superoxide dismutase (SOD) and glutathione reductase (GR) activities have significantly reduced by Cd application, and the reduction rate was increased much higher as the rate of Cd application increased. On the other hand, thiol and malondialdehyde (MDA) content were significantly increased at the application of 0.6 mM of Cd. At the second year experiment, a general increase in chlorophyll and carotenoid content was observed with Cd treatment while SOD and GR activities showed a relative reduction compared to the control. Similar to the first year measurement, thiol and MDA contents also increased considerably due to Cd treatment. At harvest, dry matter was significantly reduced by Cd treatment especially at the rate of 0.6 mM Cd, but dry yield of P. thunbergii treated with 0.3 mM Cd was less affected and it was comparable with the control seedling. Cadmium concentration in seedling tissues increased with increasing Cd application rate while Cd uptake was higher in seedlings supplied with 0.3 mM Cd, which could be ascribed to their high dry matter. Overall, our study has demonstrated the unique physiological response of P. thunbergii to Cd-prolonged exposure by showing that the changes in photosynthetic pigment content and antioxidative enzyme activities were dependent on the concentration and duration of treatment. In addition, our results have demonstrated the potential of P. thunbergii to withstand up to 0.3 mM Cd (equivalent to cumulative Cd concentration of 134.4 to 268 mg $kg^{-1}$) without showing growth reduction, hence it might be used for phytoremediation of Cd contaminated areas.
Park, Myung-Su;Gadagi, Ravi;Singvilay, Olayvanh;Kim, Chung-Woo;Chung, Hee-Kyung;Ahn, Ki-Sup;Sa, Tong-Min
Korean Journal of Environmental Agriculture
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v.20
no.5
/
pp.335-339
/
2001
Two successive in vitro experiments were carried out to examine the effect of MPS bacterial inoculation on growth, and nitrogen and phosphorus accumulation of maize plants under greenhouse condition in the same soil. There were four treatments, uninoculated control and three phosphate solubilizing bacterial inoculations, viz., Pseudomonas striata, Burkholderia cepacia and Serratia marcescens. The inoculated plants showed the higher plant height, total dry mass, nitrogen and phosphorus accumulation when compared to uninoculated control plants in both experiments. In the combined data analysis from two experiments, the plants inoculated with P. striata and B. cepacia showed significantly higher plant height, total dry mass and P accumulation when compared to S. marcescens inoculated plant and uninoculated control plants. The P. striata and B. cepacia inoculation enhanced total dry matter accumulation by 14% and phosphorus accumulation by 25% over the uninoculated control plants. The nitrogen and phosphorus concentration of maize plants were also increased due to MPS bacterial inoculation, however, the effect was not significant.
In this study, improving the wash and rubbing fastness of a natural coloring matter from Madder extract dyed on Tencel nonwoven. The cationic finishing agent(RBP), nonionic finishing agent(HPX) and mordant(PAW) were used to improving the color fastness. The two types(exhaustion and pad-dry-cure) finishing process were investigated with various finishing agent concentration. The color strength, wash and rubbing fastness of Tencel nonwoven dyed with Madder extract have been evaluated by various dye concentration and finishing agent. The exhaustion process treated with the cationic finishing agent(RBP) was effective to improving washing and rubbing fastness.
The in vitro culture of rice panicles is a culturing technique only panicle without other organs in culture solution containing organic substance, so that would be useful to study how assimilate supply affects grain development and maturation. To find the optimum stage for in vitro culture, rice panicles grown in greenhouse were sampled periodically after anthesis and cultured in nutrient medium. The panicles older than 1 weeks after anthesis had produced normal grains. Grain-filling was apparently dependent upon sucrose concentration (8-12 %) in medium, but not affected by nitrogen concentration supplied with glutamine. As far as rice panicle was supplied with sucrose and N in nutrient medium, grains continued accumulation of dry matter and maturation regardless to light condition. Considerably, grain-filling was improved when panicles were positioned horizontally inside flask, so that each grain was partially submerged to nutrient medium.
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