Investigation was carried out on response of Tetrapleura tetraptera (Schum. and Thonn.) to soil, water and light with the view of its domestication and introduction to different ecological regions. The experiment was arranged in a factorial experiment of $3{\times}3{\times}3$ in a completely randomized design (CRD) with three replicates. The factors were: soil textural class (Loamy sand, Sand and Sandy clay loam), watering regime (daily, twice a week and once a week) and light intensity (100%, 75% and 50%). Soil textural classes had significant influence on collar diameter, stem height, number of leaflets, root/shoot ratio and relative growth rate of Tetrapleura seedlings. Seedlings grown on loamy sand recorded the highest mean value- 2.28 mm for collar diameter, stem height- 12.9 cm, number of leaflets- 19.9, chlorophyll b- $0.34mg\;mL^{-1}$, leaf relative water content- 27.4% and relative growth rate- $0.037mg\;g^{-1}\;day^{-1}$. Watering regime had significant influence on the collar diameter of Tetrapleura. Seedlings watered daily recorded the highest mean value- 2.25 mm for collar diameter. Light intensity significantly influenced collar diameter and root/shoot ratio. Seedlings exposed to 100% light intensity recorded higher mean value for collar diameter- 2.28 mm and root/shoot ratio- 1.481 cm. The interaction between soil textural class and light intensity significantly affected collar diameter, stem height and number of leaflets. Higher mean value for collar diameter (2.47 mm) stem height (13.25 cm) and number of leaflets (21.16) were recorded while the interaction between soil textural class, light intensity and watering regime was significant for only number of leaflets. Tetrapleura exhibited some level of tolerance to different soil texture, drought and light intensity. Therefore, Tetrapleura has the potentials to be raised in different ecological zones characterized by difference in soil, rainfall and amount of sunshine.
For evaluating the effect of various artificial planting soil properties on the Euonymus fortunei 'Emelad'n Gold' growth, a container green wall system experiment was conducted in a wall of greenhouse at Konkuk University, Glocal campus. The experimental artificial planting grounds were prepared with different organic soil conditioner ratios (Control, $A_4O_1$, $A_2O_1$ and $A_1O_1$) and with drought tolerance and an ornamental value Euonymus fortunei 'Emelad'n Gold' was planted. The soil and plant characteristics were investigated from April to Jun 2010. The volumetric soil moisture contents were significantly increasing order as the amount of organic soil conditioner level increased in order to $A_1O_1$ > $A_2O_1$ > $A_4O_1$ > Control. At 4 treatment, soil chemical properties were inversely related to organic soil container ratios increase. The differences of root collar caliper, number of branch, and survival rate between the organic soil conditioner ratio were not significantly affected by organic soil conditioner. But, plant height, internode length, leaf length and leaf width were significantly shorter on plants planted $A_1O_1$ than plants planted other treatments. Therefore, Euonymus fortunei 'Emelad'n Gold' had good growth response regardless of organic soil conditioner ratio and the plant is expected to be a highly valuable shrub for the green wall system if it should be considered in integration with stormwater retention or as a soil conditioner for increasing soil water contents in artificial planting soil.
Proceedings of the Korean Society of Crop Science Conference
/
2017.06a
/
pp.149-149
/
2017
The RING (Really Interesting New Gene) finger proteins are known to play crucial roles in various abiotic stresses in plants. In this study, we report on RING finger E3 ligase, ${\underline{O}ryza}$${\underline{s}ativa}$${\underline{s}alt$-, ${\underline{A}BA}$- and ${\underline{d}rounght}$ stress-${\underline{i}nduced}$ RING finger ${\underline{p}}rotein{\underline{1}}$ gene (OsSAD1). In vitro ubiquitination assay demonstrated that unlike OsSAD1, a single amino acid substitution ($OsSAD1^{C168A}$) of the RING domain showed no E3 ligase activity, supporting the notion that the activity of most E3s is specified by a RING domain. Result of Yeast-Two hybridization, In vivo protein degradation assay supports that OsSAD1 interacting with 3 substrate, OsSNAC2, OsGRAS44 and OsPIRIN1, and mediates proteolysis of 3 substrates via the 26S proteasome pathway. Subcellular localizations of OsSAD1 while approximately 62% of transient signals were detected in cytosol, 38% of signals were showed nucleus. However, transiently expression of OsSAD1 was detected in cytosol 30% while as 70% of nucleus under 200 mM salt treated rice protoplasts. Results of bimolecular fluorescence complementation (BiFC) showed that two nucleus-localized proteins (OsSNAC2 and OsGRAS44) interacted with OsSAD1 in the both cytosol and nucleus. Heterogeneous overexpression of OsSAD1 Heterogeneous overexpresssion of OsSAD1 in Arabidopsis exhibited sensitive phenotypes with respect to Salt-, mannitol-responsive seed germination, seedling growth. In ABA conditions, OsSAD1 overexpression plants showed highly tolerance phenotypes, such as root length and stomatal closure. Our findings suggest that the OsSAD1 may play a negative regulator in salt stress response by modulating levels of its target proteins.
Nyamushamba, G.B.;Mapiye, C.;Tada, O.;Halimani, T.E.;Muchenje, V.
Asian-Australasian Journal of Animal Sciences
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v.30
no.5
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pp.603-621
/
2017
The current review focuses on characterization and conservation efforts vital for the development of breeding programmes for indigenous beef cattle genetic resources in Southern Africa. Indigenous African cattle breeds were identified and characterized using information from refereed journals, conference papers and research reports. Results of this current review reviewed that smallholder beef cattle production in Southern Africa is extensive and dominated by indigenous beef cattle strains adaptable to the local environment. The breeds include Nguni, Mashona, Tuli, Malawi Zebu, Bovino de Tete, Angoni, Landim, Barotse, Twsana and Ankole. These breeds have important functions ranging from provision of food and income to socio-economic, cultural and ecological roles. They also have adaptive traits ranging from drought tolerant, resistance to ticks and tick borne diseases, heat tolerance and resistance to trypanosomosis. Stakeholders in the conservation of beef cattle were also identified and they included farmers, national government, research institutes and universities as well as breeding companies and societies in Southern Africa. Research efforts made to evaluate threats and opportunities of indigenous beef cattle production systems, assess the contribution of indigenous cattle to household food security and income, genetically and phenotypically characterize and conserve indigenous breeds, and develop breeding programs for smallholder beef production are highlighted. Although smallholder beef cattle production in the smallholder farming systems contributes substantially to household food security and income, their productivity is hindered by several constraints that include high prevalence of diseases and parasites, limited feed availability and poor marketing. The majority of the African cattle populations remain largely uncharacterized although most of the indigenous cattle breeds have been identified.
Andropogon gayanus is an important grass due to its high biomass production, drought tolerance and favorable growth on low fertility acidic soils. Currently, there is little research on the impact of growth stage on the nutritional quality or the degree of $CH_4$ production that may arise from this forage during ruminal fermentation. The objectives of this study were to determine the effects of regrowth stage of A. gayanus on its chemical composition, in vitro production of gas and CH4, as well as in vitro dry matter (DM) digestibility when grown under tropical Brazilian conditions and conserved as hay or as silage. The nutritional value of A. gayanus grass declined with increasing maturity; however digestible DM yield linearly increased. After 112 d of regrowth, A. gayanus produced higher quality silage (higher lactate and lower pH and butyrate content) and higher DM yield. However, the low levels of crude protein at this time would make protein supplementation a necessity for proper rumen fermentation. No differences in $CH_4$ kinetic parameters were found with advancing maturity or preservation method (hay or silage).
Occurrence frequency of flood and drought tends to increase in last a few decades, leading to social and economic damage since the abnormality of climate changes is one of the causes for hydrologic facilities by exceedance its designed tolerance. Soil and Water Assessment Tool (SWAT) model was used in the study to estimate temporal variance of groundwater recharge and baseflow. It was limited to consider recession curve coefficients in SWAT model calibration process, thus the recession curve coefficient was estimated by the Baseflow Filter Program (BFLOW) before SWAT model calibration. Precipitation data were estimated for 2014 to 2100 using three models which are GFDL-ESM2G, IPSL-CM5A-LR, and MIROC-ESM with Representative Concentration Pathways (RCP) scenario. SWAT model was calibrated for the Soyang watershed with NSE of 0.83, and $R^2$ of 0.89. The percentage to precipitation of groundwater recharge and baseflow were 27.6% and 17.1% respectively in 2009. Streamflow, groundwater recharge, and baseflow were estimated to be increased with the estimated precipitation data. GFDL-ESM2g model provided the most large precipitation data in the 2025s, and IPSL-CM5A-LR provided the most large precipitation data in the 2055s and 2085s. Overall, groundwater recharge and baseflow displayed similar trend to the estimated precipitation data.
Korean Journal of Agricultural and Forest Meteorology
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v.3
no.4
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pp.220-237
/
2001
Over the 20th century global temperature increase has been 0.6$^{\circ}C$. The globally averaged surface temperature is projected to increase by 1.4 to 5.8$^{\circ}C$ over the period 1990 to 2100. Nearly all land areas will have higher maximum temperature and minimum temperature, and fewer cold days and frost days. More intense precipitation events will take plate over many areas. Over most mid-latitude continental interiors will have increased summer continental drying and associated risk of drought. By 2100, if the annual surface temperature increase is 3.5$^{\circ}C$, we will have 15.9$^{\circ}C$ from 12.4$^{\circ}C$ at present. Also the annual precipitation will range 1,118-2,447 mm from 972-1,841 mm at present in Korea. Consequently the average crop periods for summer crops will be 250 days that prolonged 32 days than at present. In the case of gradual increase of global warming, an annual crop can be adapted to the changing climate through the selection of filial generations in breeding process. The perennial crops such as an apple should be shifted the chief producing place to northern or high latitude areas where below 13.5$^{\circ}C$ of the annual surface temperature. If global warming happens suddenly over the threshold atmospheric greenhouse gases, then all ecosystems will have tremendous disturbance. Agricultural land-use plan, which state that farmers decide what to plant, based on their climate-based advantages. Therefore, farmers will mitigate possible negative imparts associated with the climate change. The farmers will have application to use agricultural meteorological information system, and agricultural long-range weather forecast system for their agroecosystems management. The ideal types of crops under $CO_2$ increase and climate change conditions are considered that ecological characteristics need indispensable to accomplish the sustainable agriculture as the diversification of genetic resources from yield-oriented to biomass-oriented characteristics with higher potential of $CO_2$ absorption and primary production. In addition, a heat-and-cold tolerance, a pest resistance, an environmental adaptability, and production stability should be also incorporated collectively into integrated agroecosystem.
This paper is intented to present a information of various aspects of quality related characteristics and standards for grades in maize. Maize is world's one of the three most popular cereal crops and a primary energy supplement and can contribute up to 30, 60, and 98% of the dairy diet's protein, net energy, and starch, respectively. Maize is also processed into industrial goods by wet or dry milling. Sweet corn is a leader among vegetable crops and its production for fresh or processing markets is a major industry in many countries. Over the years, the combined efforts of breeders and geneticists, biochemists, food scientists, and others have helped bring us to the point where we understand issues related to sweet corn quality. Traditional criteria for selecting corn hybrids have been based primarily on agronomic factors, including grain production, disease resistance, drought tolerance, and storage characteristics. Little emphasis has been placed on the quality and nutritional values of corn. Although there is widespread interest for value-enhanced corns have increased tremendously in the last five years, there is limited information available on the production and comparing the quality attributes of specialty grains with those of normal yellow dent corn. Most countries have developed national maize standards, aiming to provide a framework for trade, both internal and external. Where trading involves direct choice and price negotiation in front of the commodity, grading standards are rarely employed; quality is assessed visually and is influenced by end-use, and the price is determined more by local rather than national factors. The use of an agreed standard will provide an unambiguous description of the quality of the consignment and assist in the formation of a legally-binding contract. Standards can also be seen to protect consumers rights through setting limits to the amount of unsuitable or noxious material.
Nam, Kyong-Hee;Kim, Do Young;Shin, Hee Jae;Pack, In-Soon;Park, Jung-Ho;Yoon, Won Kee;Kim, Ho Bang;Kim, Chang-Gi
Korean Journal of Agricultural Science
/
v.45
no.2
/
pp.248-257
/
2018
Overexpression of AtCYP78A7, a gene encoding a cytochrome P450 protein, has been reported to improve tolerance to drought stress in genetically modified (GM) rice (Oryza sativa L.). The aim of this study was to evaluate the potential allergenicity and acute oral toxicity of the AtCYP78A7 protein expressed in GM rice. Bioinformatics analysis of the amino acid sequence of AtCYP78A7 did not identify any similarities with any known allergens or toxins. It showed that no known allergen had more than a 35% amino acid sequence homology with the AtCYP78A7 protein over an 80 amino acid window or more than 8 consecutive identical amino acids. The gene encoding the AtCYP78A7 protein was cloned in the pGEX-4T-1 vector and expressed in E. coli. Then, the AtCYP78A7 protein was purified and analyzed for acute oral toxicity. The AtCYP78A7 protein was fed at a dose of 2,000 mg/kg body weight in mice, and the changes in mortalities, clinical findings, and body weight were monitored for 14 days after the dosing. Necropsy was carried out on day 14. The protein did not cause any adverse effects when it was orally administered to mice at 2000 mg/kg body weight. These results indicate that the AtCYP78A7 protein expressed in GM rice would not be a potential allergen or toxin.
Little millet (Panicum sumatrense) is well known for its salt and drought stress tolerance and high nutritional value, but very limited knowledge of genetic variation and genomic information is available. In this study, a total of 779 primer pairs were designed from the 22,961 EST sequences of switchgrass (Pancium virgatum), of which 48 EST-SSR markers were developed based on the trials of transferability of these primers in little millet. The EST-SSR amplicons showed reproducible single band polymorphism and produced a total of 160 alleles with an average of 3.3 alleles per locus in 37 accessions of little millet. The average values of expected and observed heterozygosities were 0.266 and 0.123, respectively. The polymorphic information content (PIC) values were observed in range of 0.026 to 0.549 with an average of 0.240. The genetic relatedness among the little millet accessions was evaluated by neighbor-joining dendrogram, which grouped all accessions into two distinct groups. The validation thus demonstrated the utility of the switchgrass EST-SSR markers in assessing genomic relationships in little millet. The findings from this study could be useful for designing strategies for the identification of diverse germplasm for conservation and future molecular breeding programs for little millet.
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