• Title/Summary/Keyword: Drosophila virilis

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A Study of the Effects of MSG and Saccharose on the Emergence Rates of Drosophila virilis (Monosodium Glutamate(MSG) 와 Saccharose가 먹초파리(Drosophila virilis)의 羽化率에 미치는 影響에 대한 硏究)

  • Kang, Soon Ja
    • The Korean Journal of Zoology
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    • v.17 no.4
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    • pp.185-195
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    • 1974
  • The present investigation was attempted to see if MSG(monosodium glutamate) and saccharose, widely used for food additivies, affect the emergence rates of Drosophila virilis. For the present experiments, the four strains(Sinchon-I, Sinchon-II, Choongju, Jeju) were used and MSG and saccharose media were prepared by adding various concentrations of MSG and saccharose to the standard medium. The results obtained were as follows. 1) The significant difference in the emergence rates of Drosophila virilis was found with the concentrations of MSG and saccharose; the emergence rates were decreased as the concentrations of MSG and saccharose were increased. 2) The effects of MSG and saccharose on the emergence rates were not significantly different among the four strains of Drosophila virilis. 3) The effect of MSG on the emergence rates was found to be greater than that of saccharose.

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Systematic Relationships Among Ten Species of Subgenus Drosophila (Diptera: Drosophilidae) in Korea (한국 초파리아속(쌍시목: 초파리과) 10종에 대한 계통적 유연관계)

  • Nam Woo Kim;Eun Young Joo
    • Animal Systematics, Evolution and Diversity
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    • v.19 no.2
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    • pp.167-176
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    • 2003
  • This study sought to observe systematic relationships through taximetrical analyses by morphological characters among the 10 species, on three species in the virilis section (D. virilis, D. tsigana and D. lacertosa) and seven species in the quinaria section (D. angularis, D. brachvnephros, D. curvispina, D. kuntzei, D. nigromaculata, D. takadai and D. unispina) of the subgenus Drosophila. In the cluster and the cladistic analysis among the members of subgenus Drosophila, 10 species was divided into the 1st group of D. virilis, D. tsigana, and D. lacertosa and the 2nd group of D. angularis, D. brachynephros, D. curvispina, D. kuntzei, D. nigromaculata, D. takadai, and D. unispina. In cluster analysis, the 2nd group had three sister groups; one sister group that clustered D. angularis and D. brachynephros then D. unispina was clustered to them, another sister group clustered D, curvispina and D. takadai then D. kuntzei was clustered to them and the other sister group of D. nigromaculata. In the 10 species, D. virilis and D. lacertosa were the first to be divided and then D. tsigana. Although 1st group which D. virilis was belonged can be determined as more primitive than the 2nd group, it seemed that this group was not the direct ancestor of the 2nd group, rather there should be another ancestor. Among the quinaria species group, D. nigromaculata was the first to be divided and D. kuntzei was the most recent species to be divided.

Phylogeny of Subgenus Drosophila (Drosophilidae: Drosophila) in Korea by Allozyme and Soluble Protein Analysis (동위효소와 수용성단백질 분석에 의한 한국 초파리아속 10종의 계통)

  • Eun Young Joo;Nam Woo Kim
    • Animal Systematics, Evolution and Diversity
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    • v.19 no.2
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    • pp.217-225
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    • 2003
  • This study was conducted to ascertain interspecific relationships by analyzing allozyme and soluble proteins of ten species in the Drosophila (Drosophila) to form a part of systematic studies of Korean drosophilids. The results of allozyme and TDE analysis showed that D. (D.) curvispina and D (D.) tsigana had the furthest genetic distance. On the other hand, the genetic distance between D (D.) angularis and D (D.) brachynephros was extremely close. And, ten species of the subgenus Drosophila can be divided into the first group of D. (D.) virilis, D. (D.) tsigana and D. (D.) lacertosa , and the second group consisted of four subgroups; the first subgroup clustered D. (D.) angularis and D (D.) brachynephros, the second subgroup clustered D. (D.) unispina and D. (D.) curvispina, the third subgroup of D (D.) takadai and D. (D.) kuntzei and the fourth subgroup of D. (D.) nigromaculata alone.

Drosophila robusta species group 2종 (D.lacertosa 와 D.sordidula)의 mtDNA 변이에 의한 종분화정도

  • 최준길;박제철
    • Animal Systematics, Evolution and Diversity
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    • v.11 no.4
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    • pp.469-477
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    • 1995
  • Drosophila lacertosa and D. sordidula are members of the robusta species group in virilis section of Drosophila. The mtDNA of both species was analyzed, using 10 restriction endonucleases. The mtDNA genome size of D. lacertosa and D. sordidula was 15.7 kbp, altogether, and the numbers of mtDNA fragment were 26 and 32, respectively. Restriction cleavage map of mtDNA in these species was constructed. The patterns of cleavage map were very different between two species and it means that speciation was taken for a long time ago.

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Analysis of Two Promoters that Control the Expression of the GTP cyclohydrolase I Gene in Drosophila melanogaster

  • Byun, Jaegoo;Yoon, Jaeseung;Baek, Kwanghee
    • Molecules and Cells
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    • v.27 no.5
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    • pp.583-589
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    • 2009
  • GTP cyclohydrolase I (GTPCH) is a key enzyme in the de novo synthesis of tetrahydrobiopterin. Previously, the Drosophila melanogaster GTPCH gene has been shown to be expressed from two different promoters (P1 and P2). In our study, the 5'-flanking DNA regions required for P1 and P2 promoter activities were characterized using transient expression assay. The DNA regions between -98 and +31, and between -73 and +35 are required for efficient P1 and P2 promoter activities, respectively. The regions between -98 and -56 and between -73 and -41 may contain critical elements required for the expression of GTPCH in Drosophila. By aligning the nucleotide sequences in the P1 and P2 promoter regions of the Drosophila melanogaster and Drosophila virilrs GTPCH genes, several conserved elements including palindromic sequences in the regions critical for P1 and P2 promoter activities were identified. Western blot analysis of transgenic flies transformed using P1 or P2 promoter-lacZ fusion plasmids further revealed that P1 promoter expression is restricted to the late pupae and adult developmental stages but that the P2 promoter driven expression of GTPCH is constitutive throughout fly development. In addition, X-gal staining of the embryos and imaginal discs of transgenic flies suggests that the P2 promoter is active from stage 13 of embryo and is generally active in most regions of the imaginal discs at the larval stages.

Comparative Study of Morphology on Male Sex Comb Variation of the Drosophila Species (Drosophila 수종의 성즐변이에 관한 형태적 비교 연구)

  • Choi, Yung-Hyun;Kwon, Yong-Won;Yoo, Mi-Ae;Lee, Won-Ho
    • Korean journal of applied entomology
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    • v.33 no.4
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    • pp.216-224
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    • 1994
  • Sex combs which lack in females are characteristic of some members of the subgenus Sophophora When present, they can be van/ from a single blistle to very extensive structures, but the meianogaster subgroup of the subgenus Sophophora have only on the f~rst tarsal segments of the fore legs. D mauritiona among the melanogaster compler of the melar~ogaster subgroup have significantly more sex comb teeth (mean 12.75) than other specie& and the yakubo complex (range of the mean 6.84-758) have less than the melanogaster complex (range of the mean 835-12.75). In sex comb tooth number of interspecd~ch ybrids among the melonogoster complex, there appeared difference u;ith parental species Thus, analysis of male sex comb tooth in FI hybrid could not exhibit the direction of hereditan/ nature. On the other hand, D auraria (the montium subgroup), D suzuki (the suzuki subgroup) and D, lutecens (the takohashii subgroup) of the melanogaster species have two sex combs on the first and second tarsal segments, and the mean number were different according to the species.

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