• Journal of Embryo Transfer
• /
• v.17 no.3
• /
• pp.211-218
• /
• 2002

These studies were carried out to investigate the effects of the addition of amino acids and FBS as source of exogenous nitrogen fixation added to medium on in vitro production of blastocyst derived from bovine follicular oocytes. The base medium was TCM-199 solution for in vitro maturation(IVM) of bovine follicular oocytes and Fer-TALP solution for in vitro fertilization(IVF) and YS solution for in vitro culture(IVC). IVC used the fertilized oocytes of 24-hr culture (day 1) after IVF. Rmbryos were cultured in drop-culture that contained 25 embryos per 10 ${mu}ell$. The results obtained are as follows: 1 The developmental rates of fertilized oocytes to blastocyst that developed from YS solution with NEAA derived from MEM alone were higher than those of YS solution without NEAA. 2. The developmental rates of fertilized oocytes to blastocyst that developed from YS solution with EAA derived RPMI 1640 alone were significantly higher than those of YS solution without EAA (p<0.05). 3. When added to EAA on day 5 after NEAA supplementation on day 1, the developmental rates of hatched blastocyst and blastocyst to hatched blastocyst were improved. 4. When removed to EAA on day 3, day 4 and day 5 from medium after NEAA and EAA supplementation on day 1. the developmental rates of blastocyst to hatched blastocyst were reduced. 5. When added to FBS as source of exogenous nitrogen fixation, the developmental rates of blastocyst and hatched blastocyst that developed from the later culture higher(day 5) than those of the early culture.

• Journal of Soil and Groundwater Environment
• /
• v.15 no.1
• /
• pp.50-56
• /
• 2010

Oil contamination soil has been one of the most environmental social issues for decades in the inside and outside of country. The law of soil environmental preservation was carried out in the 1990s and the government controlled not only soil environment management and the remediation of contaminated soil but also promoted the development of remedial technology and cleanup business of contaminated soil by national policy. In addition to agriculture areas, the main oil contaminated sites are a gas station, oil reservoir, petro-chemical complex, site of railway carriage base and military camp. The contamination-frequency of agriculture area and effect sites are low but it has significantly important area on account of producing food for human beings. Therefore, we should be concerned about oil contamination damage of agriculture area. The oil contamination damage of agriculture area influenced drop of birth and breeding since the oil directly adheres to seeds and farm products even diffusion of contaminated soil to cultivation area. The studies of the crops and the food vegetation has not enough detailed data caused by the incident of oil contamination. This study investigated the effect of oil in germination and growth of selected plant seeds. In this study, we try to verify whether the oil contamination by accidents on farmland influenced the damage of farm produce and the mutual relation both oil contaminated soil or the vegetation of crops. The impact of oil on plant development was followed by phytotoxicity assessments. The plants exhibited visual symptoms of stress, growth reduction and perturbations in developmental parameters. The increase of the degree of pollution induced more marked effects in plants, likely because of the physical effects of oil. The relationships between the phytotoxicity contents of plants and growth reduction suggest a chemical toxicity of fuel oil. In addition, while cleaned up the contaminated soil under the standard of contaminated soil we examined it was suitable for region standard and it may have practical possibility for fill material of construction of afforestation and molding soil of landfill.

• Korean Journal of Medicinal Crop Science
• /
• v.19 no.6
• /
• pp.501-507
• /
• 2011

This experiment was carried out in order to collect the basic data on the standardization of the manufacturing process of Rehmannia glutinosa Libosch. var. purpurea Makino drying. By the drying methods of R. glutinosa, the content of water, inorganic components, reducing sugar, catalpol and benzo[${\alpha}$]pyrene were investigated. The water content was 15.6~17.2% when R. glutinosa was dried by cold-warm air moisture absorption drying method (CAMAD) at $60^{\circ}C$ during 6 days. Among of the inorganic components of R. glutinosa the K content was the most followed by P, Na, Ca and Mg. The reducing sugar content of R. glutinosa by the hot air drying method (HAD) was much more than that by the CAMAD. The catalpol content of R. glutinosa was not different by the drying temperature when it was dried by the CAMAD. The catalpol content of the large size tuber (about 50.0 g/unit) showed a tendency to increase from $60^{\circ}C$ until $70^{\circ}C$ drying temperature, but that of the small size tuber(about 4.0 g/unit) was decreased as being a trend as the drying temperature high when R. glutinosa was dried by the HAD, But the catalpol content R. glutinosa had a tendency to drop significantly at drying temperature above $80^{\circ}C$. The benzo[${\alpha}$]pyrene content was little detected when R. glutinosa was dried by both the SLD and the CAMAD, and the sampling by the HAD indicated within the scope of 5 ${\mu}g/kg$ which was the scope to regulate by Korean food and drug administration. In conclusion, it seemed that an appropriate drying temperature of R. glutinosa by the CAMAD and the HAD was about $60^{\circ}C$ and about $70^{\circ}C$, respectively, when we consider the catalpol content and benzo[${\alpha}$]pyrene detection in the manufacturing process of drying R. glutinosa.

• Journal of KIISE:Information Networking
• /
• v.36 no.1
• /
• pp.12-23
• /
• 2009

Sensor networks deployed in battlefields to support military operations or deployed in natural habitats to monitor the rare wildlifes must take account of protection of the location of valuable assets(i.e., soldiers or wildlifes) from an adversary or malicious tracing as well as the security of messages in transit. In this paper we propose a routing protocol GSLP(GPSR-based Source-Location Privacy) that is capable of enhancing the location privacy of an active source node(i.e., message-originating node) in the presence of multiple dormant sources(i.e., nodes lying nearby an asset whose location needs to be secured). Extended is a simple, yet scalable, routing scheme GPSR(greedy perimeter stateless routing) to select randomly a next-hop node with a certain probability for randomizing paths and to perform perimeter routing for detouring dormant sources so that the privacy strength of the active source, defined as safety period, keeps enhanced. The simulation results obtained by increasing the number of dormant sources up to 1.0% of the total number of nodes show that GSLP yields increased and nearly invariant safety periods, while those of PR-SP(Phantom Routing, Single Path), a notable existing protocol for source-location privacy, rapidly drop off as the number of dormant sources increases. It turns out that delivery latencies of GSLP are roughly less than two-fold of the shortest path length between the active source and the destination.

• Journal of the Korean Society of School Health
• /
• v.12 no.1
• /
• pp.97-107
• /
• 1999

The purpose of the study was to ascertain the trends of future circumstances in primary education along with population change. These trends, which are subject to change in population and structure, have a great impact on the size and characteristics of primary school-aged children. Accordingly, it is imperative for the government to plan for future conditions involving primary education. The major findings of the study were as follows: 1. In the long-term primary school children aged 6-11, which accounted for 17.7% of the total population in 1970, will decline to 8.6% by the year 2000, 6.9% in 2020 and 6.4% in 2030. This drastic reduction in fertility rate is a direct result of pressure by the government to control population. 2. In 1996, the total number of classes in primary schools rose to 106,594. In the future, these numbers will actually decline. By 2003 the total number of classes will peak at 142,605, but until then drop off to 112,288 by 2030-a decrease of over 6,000. 3. The actual number of primary schools in 1997 totaled 5,721. This figure will reach its highest peak, 5,942, in 2003, but it is expected decrease later after declining by 1,263, it will bottom out at 4,679 in 2003. 4. The number of teachers at primary schools increased from 101,095 in 1970, 119, 064 in 1980, 136,800 in 1990 to 138,369 in 1995. Accordingly this means that the ratio of students to teaching staff changed for the better. By the year 2005, if teachers of specialty subjects (music, art, English, physical education) are assigned to every primary school with over 18 classrooms and the number of students per class is 30, it should improve educational surroundings. This is because it is expected that the population of primary school children will continue to grow until 2003 and then decrease. Thus, there is a need to maintain the number of primary school teachers between the years 2003 and 2030 so that the ratio of students to teachers will be reduced to 1/20.25. In considering factors related to migration which influence conditions of education, it is evident that changes have already begun. In the suburbs of Seoul, population shifts are causing overcrowding in classrooms. The government believes it would be inefficient to invest in education because fluctuating migration figures make it impossible. Accordingly, we have to be concerned about stabilizing the population throughout the entire country.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
• /
• v.4 no.1
• /
• pp.17-24
• /
• 2006

A demonstration facility for advanced spent fuel conditioning process (ACP) is under construction in KAERI. In this hot cell facility, all process equipments and materials are taken in and out only through the rear door. The working table in front of the process rear door is specially designed to be openable for the efficient use of the space. This paper presents the structural safety analysis of the openable working table, for the normal operational load condition and accidential drop condition of heavy object. Both cases are investigated through static and dynamic finite element analyses. The analysis results show that structural safety of the working table is sufficiently assured and the working table is not collapsed even when an object of 500 kg is dropped from the height of 50 cm.

• Proceedings of KOSOMES biannual meeting
• /
• 2006.05a
• /
• pp.79-83
• /
• 2006

Displayed result that handle particle contaminant and hydrospace organism included in number of ballast that is happened in ship using automatic bade washing filter. Reason that remove first contaminant that is included in number of ballast is that heighten processing effect of after processing process. of the filter. Another advantage is to drop off the solids with controlling revolution of drum screen in pretreatment filtration process. The fact that it is easy to attach and detach a several type of screen for getting the expected water quality is another advantage. Filter rotation speed at 1.0rpm is filter resistance 3.0bar and 3.0rpm is filter resistance 2.8bar. Filter out impurities from ballast water over 6.0rpm is filter resistance 26bar and 10rpm is filter resistance 2.5bar. Filtration system removal aquatic organism over $80{\mu}m$ in ballast water. This study shows that the filtration treatment system has a potential for the treatment of ballast water.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.7 no.1
• /
• pp.35-41
• /
• 2004

The yeongok stream originates at the natural park, Mt. O-dae and flows to the East Sea of korea, normally maintaining I or II grade of water quality and its average water flux is 352,100 ㎥/d. However, the typhoon 'Rusa', which occured on 31 August 2002, changed its watercourse and configuration, and the ecosystem was deeply damaged. Moreover, the hydrological characteristics were once more transformed, and the ecosystem was secondarily damaged during repair-work of destroyed bridges and elevations. After the flood disaster, the species diversity diminished 17% for attached diatom and 44% for aquatic animals. However, the earth and sand, dug from river bed during intensive repair-work throughout the entire stream, made diversity drop to 32% for the diatom and the aquatic animals were wiped out. Especially, fishes were totally destroyed except for some species such as Moroco oxycephalus in the upper stream. The yeongok stream has little contamination source and short water residence time due to the short length and rapid slope, and consequently a temporary deterioration of water quality caused by repair-work may be rapidly recovered, but it needs a long time to restore the damaged ecosystem.

• Journal of Animal Science and Technology
• /
• v.49 no.2
• /
• pp.287-294
• /
• 2007

The present study was to conducted the sexing efficiency and accuracy of bovine embryo by LAMP (Loop-mediated isothermal amplification) method, the development of the biopsied embryos into re- reformation and the freezability of these blastocysts by slow-freezing and vitrification. In vivo embryos were superovaluted with gonadotropin(Antorin R-10) for 4 days combined with progestrone releasing intravaginal(CIDR) insertion in Hanwoo donors, and in vitro embryos were used blastocyst embryos at Day 7 or Day 8 after post-insemination in vitro. The biopsy of bovine embryo was carried out in a 80μl drop with Ca2+-Mg2+ free D-PBS and the viability of biopsied embryos were evaluated in IVMD medium at over 12 h culture time in 5% CO2 incubator.For embryo sexing, about five or seven blastomeres were isolated from in vitro and in vivo embryos at blastocysts with microblade. and were then subjected to LAMP. The survivability of biopsied embryos were no difference in the development rate to re-formation of blastocysts between in vivo and in vitro embryos(100% and 90% respectively). The rates of sexed embryos were compared according to two groups, the female rate was lower than that the male in the in vivo and in vitro embryos(46% vs, 54% and 40% vs, 60%, respectively). However, there were no difference in the overall sexing ratio between the two groups. The survivability of frozen-thawed sexed embryos were lower in the in vitro than in vivo embryos in the slow-freezing(Group 1) and vitrification method(Group 2), (41.7% vs. 58.8% and 57.1% vs, 77.8. respectively).

• Computers and Concrete
• /
• v.22 no.3
• /
• pp.337-353
• /
• 2018

The present study focuses on examining the structural behaviour of steel-fibre-reinforced concrete (SFRC) beams under high rates of loading largely associated with impact problems. Fibres are added to the concrete mix to enhance ductility and energy absorption, which is important for impact-resistant design. A simple, yet practical non-linear finite-element analysis (NLFEA) model was used in the present study. Experimental static and impact tests were also carried out on beams spanning 1.3 meter with weights dropped from heights of 1.5 m and 2.5 m, respectively. The numerical model realistically describes the fully-brittle tensile behaviour of plain concrete as well as the contribution of steel fibres to the post-cracking response (the latter was allowed for by conveniently adjusting the constitutive relations for plain concrete, mainly in uniaxial tension). Suitable material relations (describing compression, tension and shear) were selected for SFRC and incorporated into ABAQUS software Brittle Cracking concrete model. A more complex model (i.e., the Damaged Plasticity concrete model in ABAQUS) was also considered and it was found that the seemingly simple (but fundamental) Brittle Cracking model yielded reliable results. Published data obtained from drop-weight experimental tests on RC and SFRC beams indicates that there is an increase in the maximum load recorded (compared to the corresponding static one) and a reduction in the portion of the beam span reacting to the impact load. However, there is considerable scatter and the specimens were often tested to complete destruction and thus yielding post-failure characteristics of little design value and making it difficult to pinpoint the actual load-carrying capacity and identify the associated true ultimate limit state (ULS). To address this, dynamic NLFEA was employed and the impact load applied was reduced gradually and applied in pulses to pinpoint the actual failure point. Different case studies were considered covering impact loading responses at both the material and structural levels as well as comparisons between RC and SFRC specimens. Steel fibres were found to increase the load-carrying capacity and deformability by offering better control over the cracking process concrete undergoes and allowing the impact energy to be absorbed more effectively compared to conventional RC members. This is useful for impact-resistant design of SFRC beams.