• Molecules and Cells
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• v.26 no.4
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• pp.387-395
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• 2008

A novel Tc1-like transposable element has been identified as a new DNA transposon in the mud loach, Misgurnus mizolepis. The M. mizolepis Tc1-like transposon (MMTS) is comprised of inverted terminal repeats and a single gene that codes Tc1-like transposase. The deduced amino acid sequence of the transposase-encoding region of MMTS transposon contains motifs including DDE motif, which was previously recognized in other Tc1-like transposons. However, putative MMTS transposase has only 34-37% identity with well-known Tc1, PPTN, and S elements at the amino acid level. In dot-hybridization analysis used to measure the copy numbers of the MMTS transposon in genomes of the mud loach, it was shown that the MMTS transposon is present at about $3.36{\times}10^4$ copies per $2{\times}10^9$ bp, and accounts for approximately 0.027% of the mud loach genome. Here, we also describe novel MMTS-like transposons from the genomes of carp-like fishes, flatfish species, and cichlid fishes, which bear conserved inverted repeats flanking an apparently intact transposase gene. Additionally, BLAST searches and phylogenetic analysis indicated that MMTS-like transposons evolved uniquely in fishes, and comprise a new subfamily of Tc1-like transposons, with only modest similarity to Drosophila melanogaster (foldback element FB4, HB2, HB1), Xenopus laevis, Xenopus tropicalis, and Anopheles gambiae (Frisky).

• Development and Reproduction
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• v.17 no.3
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• pp.231-240
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• 2013

Olive flounder (Paralichthys olivaceus) is one of the commercial important flatfish species in Korea. The ocular signal transduction pathway is important in newly hatched flounders because it is closely involved in the initial feeding phase thus essential for survival during the juvenile period. However, the study of gene expression during ocular development is incomplete in olive flounder. Therefore we examined the expression analysis of specifically induced genes during the development of the visual system in newly hatched flounders. We searched ocular development-involved gene in the database of expressed sequence tags (ESTs) from olive flounder eye and this gene similar to arrestin with a partial sequence homology. Microscopic observation of retinal formation corresponded with the time of expression of the arrestin gene in the developmental stage. These results suggest that arrestin plays a vital role in the visual signal transduction pathway of the retina during ocular development. The expression of arrestin was strong in the ocular system during the entirety of the development stages. Our findings regarding arrestin have important implications with respect to its biological role and evolution of G-protein coupled receptor (GPCR) signaling in olive flounder. Further studies are required on the GPCR-mediated signaling pathway and to decipher the functional role of arrestin.

• Development and Reproduction
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• v.17 no.3
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• pp.221-229
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• 2013

Cathepsins are members of the multigene family of lysosomal cysteine proteinases and have regulated function in several life processes. The potential role of cathepsin F cysteine gene was expected as protease in the yolk processing mechanism during early developmental stage, but expression analysis was unknown after fertilization. The alignment analysis showed that amino acid sequence of cathepsin F from olive flounder liver expressed sequence tag (EST) homologous to cathepsin F of other known cathepsin F sequences with 87-98% identity. In this study, we examined the gene expression analysis of cathepsin F in various tissues at variety age flounder. Tissue distribution of the cathepsin F mRNA has been shown to be ubiquitous and constitutive pattern regardless of age in each group, although derived from cDNA library using liver sample. The mRNA level of cathepsin F more increased as developmental proceed during embryogenesis and early developmental stage, especially increased in the blastula, hatching stage and 3 days post hatching (dph). As a result, it may suggest that the proteolysis of yolk proteins (YPs) has been implicated as a mechanism for nutrient supply during early larval stages in olive flounder.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.2
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• pp.245-249
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• 2004

In this paper, we have proposed a clock recovery algorithm of Orthogonal Frequency Division Multiplexing/Quadrature Phase Shift Keying Modulation-Digital Microwave Radio(OFDM/QPSK-DMR) system using Band Limited-Pulse Shaping Filter(BL-PSF) and compared the clock phase error variance of OFDM/QPSK-DMR system with that of single carrier DMR system. The OFDM/QPSK-DMR system using windowing method requires training sequence or Cyclic Prefix (CP) to synchronize the clock phase of received signal. But transmit efficient is increased in our proposed DMR system because of no using redundant data such as training sequence or CP. The proposed clock recovery algorithm is simply realized in the OFDM/QPSK-DMR system using BL-PSF. The simulation results confirm that the proposed clock recovery algorithm has the same clock phase error variance performance in a single carrier DR system under Additive White Gaussian Noise(AWGN) environment.

• Development and Reproduction
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• v.24 no.2
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• pp.101-111
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• 2020

Coiled-coil domain containing 110 (CCDC110, KM-HN-1) is a protein containing C-terminal coiled-coil domain (CCD) which was previously discovered as a member of the human cancer/testis antigen (CTA). In addition, CCDC110 has both nuclear localization signal sequence and the leucine zipper motif. Although the functional role of CCDC110 has yet to be fully identified, the mRNA expression levels of CCDC110 are known to be highly elevated in various cancer types including testis, implying its relevance to cancer pathogenesis. In this study, we first developed several monoclonal antibody (mAb) hybridoma clones targeting CCDC110 and further isolated clone by characterizing for its specificity using immunoblotting and immunoprecipitation approaches with basal parenchymal sperm cells in testis tissue. Next, using these mAbs, we showed that the Tet-inducible overexpression of CCDC110 protein delayed the entry of G2/M phase in U2-OS osteosarcoma cells. Based on these results, we propose that CCDC110 plays a crucial role in cell cycle progression.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.1
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• pp.65.2-65.2
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• 2016

We present a new database of our two-dimensional bulge-disk decompositions for 14,482 galaxies drawn from SDSS DR12 in order to examine the properties of bulges residing in the local universe (0.005 < z < 0.05). We performed decompositions in g and r bands by utilizing the GALFIT software. The bulge colors and bulge-to-total ratios are found to be sensitive to the details in the decomposition technique. The g-r colors of bulges derived are almost constantly red regardless of bulge size except for the bulges in the low bulge-to-total ratio galaxies (approximately $B/T_r{\leq}0.3$). Bulges exhibit similar scaling relations to those followed by elliptical galaxies, but the bulges in galaxies with lower bulge-to-total ratios clearly show a gradually larger departure in slope from the elliptical galaxy sequence. The scatters around the scaling relations are also larger for the bulges in galaxies with lower bulge-to-total ratios. Both the departure in slopes and larger scatters are likely originated from the presence of young stars. While bulges seem largely similar in optical properties to elliptical galaxies, they do show clear and systematic departures as a function of bulge-to-total ratio. The stellar properties and perhaps associated formation processes of bulges seem much more diverse than those of elliptical galaxies.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.73.1-73.1
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• 2015

Isolated galaxies in low-density regions are significant in the sense that they are least affected by the hierarchical pattern of galaxy growth and interactions with perturbers at least for the last few Gyr. To form a comprehensive picture of the star formation history of isolated galaxies, we construct a catalog of isolated galaxies and their comparison sample in relatively denser environments. The galaxies are drawn from SDSS DR7 in the redshift range of 0.025 < z < 0.044. We performed visual inspection and classified their morphology following the Hubble classification scheme. We have investigated the color-magnitude diagram and found elliptical and unbarred spiral galaxies in isolated systems are relatively fainter and bluer than those in denser regions. For the spectroscopic study, we make use of the OSSY catalog (Oh et al. 2011). Our analysis on the absorption-line properties based on the comparison with stellar population models suggests that isolated elliptical galaxies are likely to be younger and metal poorer, while isolated Sc-type galaxies seem to have older luminosity-weighted ages, than their high-density counterpart. In addition, according to the BPT diagnostics, early-type galaxies among isolated galaxies are rather evenly classified into star forming, composite, Seyfert and LINER, whereas their comparisons are mainly populated in the LINER region. On the other hand, late-type galaxies do not show any prominent difference. We discuss the evolutionary histories of isolated galaxies in the context of the standard ${\Lambda}CDM$ cosmology.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.70.1-70.1
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• 2011

We have investigated properties of merging galaxies in the nearby universe, using Sloan Digital Sky Survey (SDSS) DR7. We first constructed two galaxy samples according to redshift range: Sample 1 for 0 ${\leq}$ z ${\leq}$ 0.025 and Sample 2 for 0.09 ${\leq}$ z ${\leq}$ 0.1. We then identified 118 and 184 merging galaxies among the galaxies in the Sample 1 and 2, respectively, and classified them into different merging types and stages by visual inspection of galaxy images. In the Sample 1, there are more wet mergers than dry mergers, while most merging galaxies in the Sample 2 are dry mergers. The color-magnitude diagram of the merging galaxies in our samples is comparable to that of normal galaxies. Dry mergers tend to locate in the red sequence, while wet and mixed mergers reside mostly in the blue cloud. Unlike some previous studies, we did not find a clear trend that the merger rate increases at higher redshift. However, it is difficult to make a direct comparison of the merger rate found in different studies, because it depends on the number of observed galaxies and criteria for merger classification. From the ratios of emission lines, we infer that the faction of merging galaxies with AGNs is higher in wet mergers than in other types.

• Development and Reproduction
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• v.27 no.1
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• pp.39-46
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• 2023

Pumilio (Pum) is an RNA-binding protein and translational repressor important to diverse biological processes. In the Drosophila ovary, Pum is expressed in female germline stem cells (GSCs), wherein it acts as an intrinsic stem cell maintenance factor via repressing target mRNAs that are as yet mostly unknown. Pum recognizes the Pum binding sequence (PBS) in the mRNA 3'UTR through its C-terminus Puf domain. Translational repression is mediated through its N-terminal domain, but the molecular mechanism remains largely unknown. We previously showed that Bag-of-marbles, a critical differentiation-promoting factor of female GSCs, physically interacts with the N-terminus of Pum. We further showed that this interaction is critical to Bam inhibition of Pum repressive action in cultured cells, but the physiological relevance was not addressed. Here we design an in vivo GFP translational reporter bearing the PBS in its 3'UTR and show that GFP expression is reduced in cells wherein Pum is known to be active. Furthermore, we demonstrate in pum mutant ovary that this GFP repression requires Pum, and also that the sensor faithfully monitors Pum activity. Finally, we show that forced expression of Bam inhibits Pum-mediated repression, validating that Bam inhibits Pum activity in vivo.

• Korean Journal of Microbiology
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• v.28 no.1
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• pp.1-5
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• 1990

The coding sequence of hepatitis B viral core antigen (HBcAg) (subtype adr) contains two in-phase initiation codons, one for precore and the other for core antigen gene. To study the expression of core antigen and the role of precore region, the coding sequence of HBcAg with or without precore (pre-C) region were subcloned into yeast expression vector containing phosphoglycerate kinase (PGK) promoter. To study the role of upstream region in the expression of the core antigen, a series of 5' deletion mutants were also subcloned into the vector. After transformation into various host strains, the expression of HBcAg were analysed by radio-immunoassat. Under optimal condition of core antigen gene expression in yeast, the highest amount of antigen was detected in the cell line SHY4 containing pGKHBc plasmid composed of the yeast PGK gene promoter, terminator and C-gene. Regardless of the presence of precore region, core antigen was not detected in the medium but in cell extract. These results suggest that precore region cannot affect the secretion of core antigen in Saccharomyces cerevisiae.