• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제24권1호
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• pp.1-6
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• 2021

Next-generation sequencing (NGS) technologies have changed the process of genetic diagnosis from a gene-by-gene approach to syndrome-based diagnostic gene panel sequencing (DPS), diagnostic exome sequencing (DES), and diagnostic genome sequencing (DGS). A priori information on the causative genes that might underlie a genetic condition is a prerequisite for genetic diagnosis before conducting clinical NGS tests. Theoretically, DPS, DES, and DGS do not require any information on specific candidate genes. Therefore, clinical NGS tests sometimes detect disease-related pathogenic variants in genes underlying different conditions from the initial diagnosis. These clinical NGS tests are expensive, but they can be a cost-effective approach for the rapid diagnosis of rare disorders with genetic heterogeneity, such as the glycogen storage disease, familial intrahepatic cholestasis, lysosomal storage disease, and primary immunodeficiency. In addition, DES or DGS may find novel genes that that were previously not linked to human diseases.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2022년도 춘계학술대회
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• pp.674-675
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• 2022

본 논문에서는 차량 정차해있거나 저속 주행 중 차량 사각지대에 위치한 객체(보행자, 자전거, 이륜차) 인식 가능하고 초당 30프레임의 빠른 객체 인식 성능을 갖는 DPS(FPGA) 기반 객체 인식 방법을 제시한다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1986년도 하계학술대회논문집
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• pp.238-241
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• 1986

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.544-544
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• 2003

• 동력기계공학회지
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• 제16권2호
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• pp.66-74
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• 2012

In this paper, the authors propose a new approach to control a barge type surface vessel. It is based on the Dynamic Positioning System(DPS) design. The main role of barge ship is to carry and supply the materials to the floating units and other places. To carry out this job, it should be positioned in the specified area. However sometimes the thrust systems are installed on it, and in general the rope control by mooring winch system is used. It may be difficult to compare the control performances of two types. If we consider this problem in point of usefulness, we can easily find out that the winch control system is more useful and applicable to the real field than the thrust control system except a special use. Therefore, in this paper we consider a DPS design problem which can be extended to the many application fields. The goal of this paper is twofold. First, the sliding mode controller (SMC) for positioning the our vessel is proposed. Especially, in this paper, a robust stability condition is given based on descriptor system representation. In the result, the sliding mode control law guarantees to keep the vessel in the defined area in the presence of environmental disturbances. And second, the thrust allocation problem is solved by using redistributed pseudo-inverse (RPI) algorithm to determine the thrust force and direction of each individual actuator. The proposed approach has been simulated with a supply vessel model and found work well.

• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.288-295
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• 2015

Salmonella enterica serovar Enteritidis is the predominant agent causing salmonellosis in chickens and other domestic animals. In an attempt to identify antigenic S. Enteritidis outer membrane proteins (OMPs) that may be useful for subunit vaccine development, we established a proteomic map and database of antigenic S. Enteritidis OMPs. In total, 351 and 301 spots respectively from S. Enteritidis strain 270 and strain 350 were detected by two-dimensional gel electrophoresis. Fifty-one antigen-reactive spots were detected by antisera on two-dimensional immunoblots and identified as 12 specific proteins by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. OmpA and DNA starvation/stationary phase protection protein (Dps) were the most abundant proteins among the identified OMPs, comprising 22 and 12 protein species, respectively. Interestingly, we found that the Dps of S. Enteritidis is also antigenic. OmpW was also verified to have high antigenicity. These results show that OmpA, Dps, and possibly OmpW are antigenic proteins. This study provides new insights into our understanding of the immunogenic characteristics of S. Enteritidis OMPs.

• The Plant Pathology Journal
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• 제29권4호
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• pp.374-385
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• 2013

Environmental stresses induce several plant pathogenic bacteria into a viable but nonculturable (VBNC) state, but the basis for VBNC is largely uncharacterized. We investigated the physiology and morphology of the copper-induced VBNC state in the plant pathogen Ralstonia solanacearum in liquid microcosm. Supplementation of $200{\mu}M$ copper sulfate to the liquid microcosm completely suppressed bacterial colony formation on culture media; however, LIVE/DEAD BacLight bacterial viability staining showed that the bacterial cells maintained viability, and that the viable cells contain higher level of DNA. Based on electron microscopic observations, the bacterial cells in the VBNC state were unchanged in size, but heavily aggregated and surrounded by an unknown extracellular material. Cellular ribosome contents, however, were less, resulting in a reduction of the total RNA in VBNC cells. Proteome comparison and reverse transcription PCR analysis showed that the Dps protein production was up-regulated at the transcriptional level and that 2 catalases/peroxidases were present at lower level in VBNC cells. Cell aggregation and elevated levels of Dps protein are typical oxidative stress responses. $H_2O_2$ levels also increased in VBNC cells, which could result if catalase/peroxidase levels are reduced. Some of phenotypic changes in VBNC cells of R. solanacearum could be an oxidative stress response due to $H_2O_2$ accumulation. This report is the first of the distinct phenotypic changes in cells of R. solanacearum in the VBNC state.

• 한국전자파학회논문지
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• 제18권2호
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• pp.118-125
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• 2007

본 논문에서는 평행 결합 버섯 구조를 이용하여 대역 통과 필터의 고조파 성분을 제거하였다. Double positive(DPS)전송 선로인 마이크로스트립 라인과double negative(DNG)전송 선로인 버섯 구조 사이에서는 격리된 마이크로스트립 라인과 버섯 구조의 교차되는 분산 곡선 주파수 대역에서 강한 결합이 복소 전파 상수로 인해서 발생한다. 교차되는 분산 곡선 주파수에서 강한 결합으로 인하여 전파가 전파를 하지 못하기 때문에 이와 같은 구조는 대역 저지 필터로 이용할 수 있는 것이다. 제안된 대역 저지 필터는 공진기를 이용한 형태가 아니라 평행 결합 전송 선로를 이용하였기 때문에 넓은 대역폭을 가지고 DPS와 DNG 전송 선로 사이의 강한 결합으로 인해서 소형화에 유리하다는 장점을 가지고 있다. 본 논문에서는 중심 주파수 4 GHz, 3 dB fractional 대역폭은 40%인 평행 결합 대역 저지 필터를 설계하였고, 이를 두 가지 종류의 대역 통과 필터의 고조파 모드를 억제하는데 이용하였다.

• 대한전자공학회논문지TC
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• 제45권8호
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• pp.69-74
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• 2008

본 논문에서는 유전율($\varepsilon'$)과 투자율($\mu'$)이 양수인 double-positive (DPS) 물질에서 정의된 전파 상수를 epsilon-negative(ENG) 물질 (${\varepsilon}'<0,\;{\mu}'>0$), mu-negative (MNG) 물질 (${\varepsilon}'>0,\;{\mu}'<0$), 그리고 double-negative (DNG) 물질 (${\varepsilon}'<0,\;{\mu}'<0$)으로 확장하여 유도하였다. 이와 함께 유전율 손실(${\varepsilon}"$)과 투자율 손실(${\mu}"$)이 전파 상수에 미치는 영향을 분석하여 이 물질에서 전파가 right-handed (RH) 혹은 left-handed (LH)로 동작하는지를 규명하였다. DPS와 DNG 물질은 ${\varepsilon}"$와 ${\mu}"$의 값에 상관없이 각각 RH 그리고 LH 매질로 동작한다. 반면 ENG와 MNG 물질은 (${\varepsilon}'{\mu}"+{\varepsilon}"{\mu}'$)의 부호가 양수이면 RH, 음수이면 LH 매질로 동작하는 특이한 특성을 가진다.

• 한국환경농학회지
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• 제34권3호
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• pp.217-222
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• 2015

BACKGROUND: Garlic (Allium sativum) contains polyphenols and sulfur compounds that are recognized as antioxidant, antithrombotic, anticancer, antibacterial, antimicrobial, nematicidal, and insecticidal activity. For this reason, the Environmentally-friendly Agriculture Promotion Act allowed the garlic extract as commercial biopesticide material for crop protection, nine commercial biopesticides containing A. sativum extract have been marketed in Korea. METHODS AND RESULTS: The determination of allylmethyl sulfide (AMS), dimethyl disulfide (DMDS), and dipropyl sulfide (DPS) in biopesticides containing A. sativum extract was developed and validated by gas chromatography (GC) mass spectrometry (MS) with head-space sampler. The developed method was validated, and the limit of quantification (LOQ) and recovery rates of AMS, DMDS, and DPS were 0.08, 0.32, and 0.09 mg/L and 90.3-91.3, 86.2-88.3, and 87.6-89.5%, respectively. From the nine commercial biopesticide samples, contents of AMS, DMDS, and DPS were analyzed using the developed method and results showed