• Title/Summary/Keyword: Dps

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Vitrification and Ultrarapid Freezing of Day 2 Mouse Embryos (제 2일째 생쥐 배아의 초자화동결과 초급속동결)

  • Yang, Jung-Sook;Sohn, Cherl;Bae, In-Ha
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.3
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    • pp.283-289
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    • 2000
  • Objective: The study was performed to compare the survival rate and the development of day 2 mouse embryos which had freezing procedures done. Methods: We used three different vitrification solutions (EFS, VS14, DPS) and a ultrarapid freezing solution (UFS) for cryopreservation of day 2 mouse embryo. Results: We tested toxicity by exposing embryos to vitirification solutions and a ultrarapid freezing solution. The survival rates are 100%, 97.8%, 95.6% and 100% (EFS, VS14, DPS and UFS). After cultured for 96 hours, hatching rates of each group are 93.5% (no freezing), 95.6% (EFS), 86.4% (VS14), 93.0% (DPS), and 93.0% (UFS). There is no significant differences among groups. The survival rates after thawing cryopreserved embryos are 80.2%, 91.7%, 69.5%, 0% and 91.8% (slow freezing, EFS, VS14, DPS and UFS). Also cultured for 96 hours, the hatching rates are 93.5% (no freezing), 84.1% (slow freezing), 93.9% (EFS), 48.5% (VS14) and 70.1% (UFS). Conclusion: The survival rates of vitrification in EFS solution and ultrarapid freezing are higher than slow freezing (p<0.05). The hatching rate of vitrification in EFS solution cultured for 96 hours is highest, so vitrification of day 2 mouse embryos in EFS solution considered as more effective for cryopreservation.

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A Channel Access Control algorithm and a Dynamic Slot Allocation algorithm for rt_VBR services and ABR services in Wireless ATM Networks (무선 ATM망에서 rt_VBR 및 ABR 서비스를 위한 채널 접속 제어 알고리즘과 동적 대역 할당 알고리즘)

  • Yang, Seong-Ryoung;Im, In-Taek;Heo, Jeong-Seok
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.28 no.3B
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    • pp.191-199
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    • 2003
  • In this paper, a channel access control algorithm and a dynamic slot allocation algorithm are proposed for rt_VBR services and ABR services in the wireless ATM networks. In the proposed algorithm, rt_VBR terminals that require real-time services transmit a reservation request packet by a RAS minislot. An rt_VBR terminal, which transmitted the reservation request packet, transmits the dynamic parameters by DPS minislot without contention. On the other hand, ABR terminals that have a non-real-time traffic burst transfer a reservation request packet with contention basis. Based on the received dynamic parameters, the base station scheduler allocates uplink data slots as well as DPS minislot into rt_VBR terminals.

Reactive Power and Soft-Switching Capability Analysis of Dual-Active-Bridge DC-DC Converters with Dual-Phase-Shift Control

  • Wen, Huiqing;Su, Bin
    • Journal of Power Electronics
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    • v.15 no.1
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    • pp.18-30
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    • 2015
  • This paper focuses on a systematical and in-depth analysis of the reactive power and soft-switching regions of Dual Active Bridge (DAB) converters with dual-phase-shift (DPS) control to achieve high efficiency in a wide operating range. The key features of the DPS operating modes are characterized and verified by analytical calculation and experimental tests. The mathematical expressions of the reactive power are derived and the reductions of the reactive power are illustrated with respect to a wide range of output power and voltage conversion ratios. The ZVS soft-switching boundary of the DPS is presented and one more leg with ZVS capability is achieved compared with the CPS control. With the selection of the optimal operating mode, the optimal phase-shift pair is determined by performance indices, which include the minimum peak or rms inductor current. All of the theoretical analysis and optimizations are verified by experimental tests. The experimental results with the DPS demonstrate the efficiency improvement for different load conditions and voltage conversion ratios.

Production of Coenzyme $Q_{10}$ by Recombinant E. coli Harboring the Decaprenyl Diphosphate Synthase Gene from Sinorhizobium meliloti

  • Seo Myung-Ji;Im Eun-Mi;Hur Jin-Haeng;Nam Jung-Yeon;Hyun Chang-Gu;Pyun Yu-Ryang;Kim Soon-Ok
    • Journal of Microbiology and Biotechnology
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    • v.16 no.6
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    • pp.933-938
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    • 2006
  • Decaprenyl diphosphate synthase (DPS) is the key enzyme for the production of coenzyme $Q_{10}$ ($CoQ_{10}$). A dps gene from Sinorhizobium meliioti KCCM 11232 (IFO 14782) was isolated by PCR and then cloned in Escherichia coli. DNA sequencing analysis revealed an open reading frame of 1,017 bp encoding a 338-amino-acid protein. The protein was identical at the 98% level to the putative octaprenyl diphosphate synthase (IspB) of S. meliloti 1021. The deduced amino acid sequence included the DDxxD domains conserved in the majority of the prenyl diphosphate synthases. Heterologous expression in E. coli BL21 (DE3) was carried out, and the $CoQ_{10}$ produced was then analyzed by HPLC. E. coli BL21 (DE3) harboring the dps gene from S. melioti produced CoQ$_{10}$ in addition to endogenous coenzyme Q$_8$ (CoQ$_8$), whereas wild-type E. coli BL21 (DE3) host did not have the ability of producing CoQ$_{10}$. The results suggest that the putative dps from S. meliloti KCTC 2353 encoded the DPS.

Power based scheduling for Collaborative MIMO system (Collaborative MIMO 시스템을 위한 전력기반 스케줄링 방식)

  • Kim, Young-Joon;Lee, Jung-Seung;Baik, Doo-Kwon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.12A
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    • pp.1209-1216
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    • 2008
  • In order to maximize spectrum efficiency and data rate MIMO(Multiple Input Multiple Output) is adopted to wireless system. OFDM-based WiMAX and LTE accommodate MIMO as mandatory technology. STC(Space Time Coding) and SM(Spatial Multiplexing) are used in downlink while in uplink C-MIMO(Collaborative MIMO) is used to improve data throughput. In this paper conventional pairing schemes, RPS(Random Pairing Scheduling) and DPS(Determinant Pairing Scheduling) are analyzed. From the analysis the performance of DPS algorithm is better than that of RPS because DPS measures orthogonal factor between paired users. However, there are potential problems such as hardware complexity and performance. To overcome the issues Power-Based Scheduling(PBS) algorithm is proposed for C-MIMO. PBS can provide higher performance compared to RPS and dramatically reduce hardware complexity compared to DPS

Aerodynamic Characteristics of the Blended-Wing-Body for the Position and Aspect Ratio of the Inlet and Outlet of an Embedded Distributed Propulsion System (Embedded Type 분산 추진 장치의 입·출구 형상 및 위치 변화에 따른 융합익기의 공력해석)

  • Kim, Hyo-Seop;Choi, Hyun-Min;Cho, Jin-Soo
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.40 no.6
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    • pp.467-474
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    • 2012
  • UAVs for reconnaissance and intelligence operations require long endurance capability, which demands high efficiency of the propulsion system. The distributed propulsion system(DPS) generates the thrust by replacing a large propulsion system with a number of small propulsion systems. A DPS distributed along the wing span can produce gains in propulsion efficiency by reducing ejection velocity. Also, the ingestion of boundary layers through the distributed DPS inlet and ejecting flow from the outlet can improve the lift to drag ratio of the vehicle. This study investigates the effects of locations and size of the inlet and outlet of the DPS on the blended-wing-body design based on Eppler 337 airfoil, with a CFD tool. The fans in the DPS are modeled as actuator disks for computational efficiency. The best location and aspect ratio of the inlet and outlet are found from lift-to-drag ratio and pitching moment considerations.

Dependence of Hardness Change on Microstructure during Isothermal Aging in Mg-Al Alloy (Mg-Al 합금에서 등온 시효 중 경도 변화의 미세조직 의존성)

  • Han, Jin-Gu;Jun, Joong-Hwan
    • Journal of the Korean Society for Heat Treatment
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    • v.32 no.6
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    • pp.249-255
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    • 2019
  • This study is intended to clarify the main microstructural factors that contribute to an increase of hardness during isothermal aging in Mg-Al alloy. For this work, Mg-9.3%Al alloy specimens were solution-treated at 688 K for 24 h followed by water quenching, and then aged at 473 K for up to 24 h. The aging at 473 K yielded nodular discontinuous precipitates (DPs) with (${\alpha}+{\beta}$) lamellar morphology at the grain boundaries, and the volume fraction of DPs increased from 0% to ~30% with increasing aging time up to 12 h. For the aging times longer than 12 h, further formation of DPs was substantially inhibited owing to the occurrence of significant continuous precipitation within the ${\alpha}-(Mg)$ matrix, and the density of continuous precipitates (CPs) becomes greater with increasing aging time. Hardness of the specimen was steadily increased with aging time up to 24 h. Microstructural examination on the aged specimens revealed that the increased overall hardness at the early stage of aging is associated with the increased volume fraction of DPs, but at the later stage of aging, where the amount of DPs was hardly changed, the increased hardness of the ${\alpha}-(Mg)$ matrix in response to the higher density of CPs within the matrix, plays a key role in increasing the overall hardness value.

Structural evaluation of degradation products of Loteprednol using LC-MS/MS: Development of an HPLC method for analyzing process-related impurities of Loteprednol

  • Rajesh Varma Bhupatiraju;Bikshal Babu Kasimala;Lavanya Nagamalla;Fathima Sayed
    • Analytical Science and Technology
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    • v.37 no.2
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    • pp.98-113
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    • 2024
  • The current investigation entails the characterization of five degradation products (DPs) formed under different stress conditions of loteprednol using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, this study developed a stable high-performance liquid chromatography (HPLC) method for evaluating loteprednol along with impurities. The method conditions were meticulously fine-tuned which involved the exploration of the appropriate solvent, pH, flow of the mobile phase, columns, and wavelength. The method conditions were carefully chosen to successfully resolve the impurities of loteprednol and were employed in subsequent validation procedures. The stability profile of loteprednol was exposed to stress degradation experiments conducted under five conditions, and DPs were structurally characterized by employing LC-MS/MS. The chromatographic resolution of loteprednol and its impurities along with DPs was effectively achieved using a Phenomenex Luna 250 mm C18 column using 0.1 % phosphoric acid, methanol, and acetonitrile in 45:25:30 (v/v) pumped isocratically at 0.8 mL/min with 243 nm wavelength. The method produces an accurate fit calibration curve in 50-300 ㎍/mL for loteprednol and LOQ (0.05 ㎍/mL) - 0.30 ㎍/mL for its impurities with acceptable precision, accuracy, and recovery. The stress-induced degradation study revealed the degradation of loteprednol under basic, acidic, and photolytic conditions, resulting in the formation of seven distinct DPs. The efficacy of this method was validated through LC-MS/MS, which allowed for the verification of the chemical structures of the newly generated DPs of loteprednol. This method was appropriate for assessing the impurities of loteprednol and can also be appropriate for structural and quantitative assessment of its degradation products.

Study on a Program for Automatic Control of on/off Switch on Modem (MODEM의 자동제어기 & 프로그램에 관한 연구)

  • Han Sang-Do
    • Journal of the military operations research society of Korea
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    • v.17 no.2
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    • pp.123-134
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    • 1991
  • one of the problems the users of DPS-6/45 used to face in the course of having access to work stations was that a telephone call has to be made manually every time to have receiver's modem on or off, and naturally it meant the waste of manpower as well as of time. A program has been worked out to automatically operate and control. utilizing the system clock within the main computer (DPS-6/45), the on/off switch on the modems. It has resulted in; 1) promotion in operability of the main computer, DPS-6/45 2) prevention of unnecessary telephone calls 3) curtailment of expenditure and manpower

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DPO 면허취득과정의 최근 동향

  • Ye, Byeong-Deok
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2015.07a
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    • pp.57-59
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    • 2015
  • 심해에서의 유전개발이 활성화되면서 이러한 개발에 필수적인 장비인 동적위치제어시스템 (Dynamic Positioning System, DPS)을 설치한 선박 또는 해양구조물의 수요도 폭발적으로 증가하고 있으며, 이에 따라 DPS 운영에 필수적인 DPO (Dynamic Positioning Operator) 자격증을 취득하고자 하는 지원자도 매년 급속하게 증가하고 있다. 그러나 DPO 교육훈련과정은 강제요건이 아니며, 따라서 엄정한 자격증명 및 검증이 어려운 실정이다. 특히 매년 2000건 이상의 신규면허를 심사하고 발급하는 것은 물론 기존 면허자에 대한 재검증 절차도 수행하여야 하는 현재의 상황으로 볼 때 효율적인 DPO 양성을 기대하기는 어려운 실정이다. 이 연구에서는 보다 이러한 문제점을 개선하여 보다 더 효율적이면서도 엄정한 DPO 양성을 위한 제도개선을 제안한다.

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