• Toxicological Research
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• v.23 no.4
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• pp.311-319
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• 2007

Elevated blood levels of homocysteine (a sulfur-containing amino acid) have been linked to increased risk of cerebrovascular disease including Alzheimer's disease. A recent study suggests that elevated homocysteine levels may lead to replicative senescence in vitro called 'permanent arrest of cell cycle' caused by oxidative stress. In this study, serum homocysteine level in rat was reduced by Lentinus edodes-powder diet, resulting in the reduced level of oxidative stress in rat brain. In addition, homocysteine-induced replicative senescence treated with or without Lentinus edodes-powder was analyzed by population doubling in vitro. The Lentinus edodes-powder induced a increased number of population doubling in primary neuron cell isolated from rat-cerebral cortex. This indicates that Lentinus edodes-powder would delay a homocysteine-induced aging of neuron cells in brain, showing a possible role in preventing cerebrovascular diseases including Alzheimer's disease.

• The Journal of the Korea institute of electronic communication sciences
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• v.10 no.7
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• pp.845-850
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• 2015

Love which is one of the emotional of mankind, has been studied in sociology and psychology as a matter of great concern. Through such a research, the researchers have provided the basic mathematical model for love model, we cannot find nonlinear characteristics through the basic love model. Therefore, in this paper, in order to find nonlinear behaviors in the basic love model, we apply external force to the basic love model. Then we confirm the existence of nonlinear behaviors through time series and phase portrait. We also confirm that this nonlinear behaviors have the periodic doubling, chaotic phenomena and periodic process which are very similar to typical chaotic occurrence phenomena.

• Journal of the Korean Mathematical Society
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• v.55 no.6
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• pp.1359-1380
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• 2018

In this paper, we prove that if a metric measure space satisfies the volume doubling condition and the Hardy type inequality with the same exponent n ($n{\geq}3$), then it has exactly the n-dimensional volume growth. Besides, three interesting applications of this fact have also been given. The first one is that we prove that complete noncompact smooth metric measure space with non-negative weighted Ricci curvature on which the Hardy type inequality holds with the best constant are isometric to the Euclidean space with the same dimension. The second one is that we show that if a complete n-dimensional Finsler manifold of nonnegative n-Ricci curvature satisfies the Hardy type inequality with the best constant, then its flag curvature is identically zero. The last one is an interesting rigidity result, that is, we prove that if a complete n-dimensional Berwald space of non-negative n-Ricci curvature satisfies the Hardy type inequality with the best constant, then it is isometric to the Minkowski space of dimension n.

• Korean Journal of Veterinary Research
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• v.24 no.2
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• pp.137-147
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• 1984

Cells with an epithelioid morphology were isolated from the rabbit myometrium and were grown in culture. The cells had a doubling time of 53 hours when grown in the presence of 10% fetal calf serum in Basal Eagle's medium with 3mM glutamine. In the presence of estrogen plus insulin, doubling time was reduced to 40 hours. Creatine kinase activity upon reaching confluency was determined to be 0.019 unit per mg protein. Approximately 30% of the activity was extractable only in high ionic strength buffer. Cells also contained plasminogen activator with a specific activity of 140 CTA units per million cells. Creatine kinase was mainly BB form. The cells contained a cross reactive protein against bovine smooth muscle uterine anti-myosin.

• Atmosphere
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• v.33 no.1
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• pp.33-47
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• 2023

In this study, for the application of observation errors to the Korean Integrated Model (KIM) to utilize the Constellation Observing System for Meteorology, Ionosphere & Climate-2 (COSMIC-2) new satellites, the observation errors were diagnosed based on the Desroziers method using the cost function in the process of variational data assimilation. We calculated observation errors for all observational species being utilized for KIM and compared with their relative values. The observation error of the calculated the Global Navigation Satellite System Radio Occultation (GNSS RO) was about six times smaller than that of other satellites. In order to balance with other satellites, we conducted two experiments in which the GNSS RO data expanded by about twice the observation error. The performance of the analysis field was significantly improved in the tropics, where the COSMIC-2 data are more available, and in the Southern Hemisphere, where the influence of GNSS RO data is significantly greater. In particular, the prediction performance of the Southern Hemisphere was improved by doubling the observation error in global region, rather than doubling the COSMIC-2 data only in areas with high density, which seems to have been balanced with other observations.

• Geomechanics and Engineering
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• v.37 no.4
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• pp.323-339
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• 2024

If the pressure exerted on the face of a tunnel excavated by TBM exceeds a threshold, it leads to failure of the soil or rock masses ahead of the tunnel face, which results in heaving the ground surface. In the current research, the upper bound method of limit analysis was employed to calculate the blow-out pressure of tunnels excavated in rock masses obeying the Hoek-Brown nonlinear criterion. The results of the proposed method were compared with three-dimensional finite element models, as well as the available methods in the literature. The results show that when σ_ci, m_i, and GSI increase, the blow-out pressure increases as well. By doubling the tunnel diameter, the blow-out pressure reduces up to 54.6%. Also, by doubling the height of the tunnel cover and the surcharge pressure exerted on the ground surface above the tunnel, the blow-out pressure increased up to 74.9% and 5.4%, respectively. With 35% increase in the unit weight of the rock mass surrounding the tunnel, the blow-out pressure increases in the range of 14.8% to 19.6%. The results of the present study were provided in simple design graphs that can easily be used in practical applications in order to obtain the blow-out pressure.

• Radiation Oncology Journal
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• v.19 no.2
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• pp.163-170
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• 2001

Purpose : The measurement of radiation survival using a clonogenic assay, the established standard, can be difficult and time consuming. In this study, We have used the MTT assay, based on the reduction of a tetrazolium salt to a purple formazan precipitate by living cells, as a substitution for clonogenic assay and have examined the optimal condition for performing this assay in determination of radiation sensitivity. Materials and Methods : Four human cancer cell lines - PCI-1, SNU-1066, NCI-H630 and RKO cells have been used. For each cell line, a clonogenic assay and a MTT assay using Premix WST-1 solution, which is one of the tetrazolium salts and does not require washing or solubilization of the precipitate were carried out after irradiation of 0, 2, 4, 6, 8, 10 Gy. For clonogenic assay, cells in $25\;cm^2$ flasks were irradiated after overnight incubation and the resultant colonies containing more than 50 cells were scored after culturing the cells for $10\~14$ days. For MTT assay, the relationship between absorbance and cell number, optimal seeding cell number, and optimal timing of assay was determined. Then, MTT assay was performed when the irradiated cells had regained exponential growth or when the non-irradiated cells had undergone four or more doubling times. Results : There was minimal variation in the values gained from these two methods with the standard deviation generally less than $5\%$, and there were no statistically significant differences between two methods according to t-test in low radiation dose (below 6 Gy). The regression analyses showed high linear correlation with the $R^2$ value of $0.975\~0.992$ between data from the two different methods. The optimal cell numbers for MTT assay were found to be dependent on plating efficiency of used cell line. Less than 300 cells/well were appropriate for cells with high plating efficiency (more than $30\%$). For cells with low plating efficiency (less than $30\%$), 500 cells/well or more were appropriate for assay. The optimal time for MTT assay was after 6 doubling times for the results compatible with those of clonogenic assay, at least after 4 doubling times was required for valid results. In consideration of practical limits of assay (12 days, in this study) cells with doubling time more than 3 days were inappropriate for application. Conclusion : In conclusion, it is found that MTT assay can successfully replace clonogenic assay of tested cancer cell lines after irradiation only if MTT assay was undertaken with optimal assay conditions that included plating efficiency of each cell line and doubling time at least.

• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.103-108
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• 2004

This study was carried out to develop the efficient methods for the subsequent progeny of intersectional hybrid between Oriental and Asiatic lily hybrids. The pollen fertility and germination ability of 3 different allotetraploids (OAOA) after somatic chromosome doubling was ranged from 0 to 80 percent on artificial pollen germination medium. The number of BC$_1$ progeny using allotetraploid of F$_1$ OA-hybrid as male and female parent was different. The efficiency of BC$_1$ progeny production was increased when F$_1$OA-hybrids was used as male rather than as female parent. And in back crosses of F$_1$ OA-hybrids with the Asiatic and Oriental hybrids, Asiatic hybrids showed higher efficiency on BC$_1$ progeny production. The ploidy level between 2x or 4x Asiatic hybrid and allotetraploid F$_1$ OA-hybrid was determined and showed higher progeny production in 2x-4x crosses rather than 4x-4x.

• Korean Journal of Plant Resources
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• v.28 no.5
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• pp.656-664
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• 2015

To produce high quality watermelon, three tetraploid watermelon breeding lines (‘SA03-1’, ‘SA06-1’ and ‘SB01-1’) were developed by treatment with different chromosome doubling reagents. To identify the optimal tetraploid inductive conditions, the three watermelon breeding lines were selected by counting the number of doubled chloroplasts in guard cells. Tetraploid induction rates differed depending on the genotypes and treatment with doubling reagents. However, the highest induction rate occurred with 1.0% colchicine (82.2%). These putative tetraploid lines were re-confirmed for ploidy using flow cytometric analysis and chromosome counting. The internode length of the tetraploid breeding lines was different when the leaf size was larger in all three tetraploid lines compared to their diploids. The fruit weight of the tetraploid fruits for ‘SA03-1’ and ‘SB01-1’ was lower than for their diploid, and the rind thickness and total sugar content (°Brix) of tetraploid SB01-1 were significantly different from those of its diploid. Tetraploid lines were sterile, yielded a lower number of seeds per fruit for ‘SA03-1’ (21), ‘SA06-1’ (62), and ‘SB01-1’ (34.7), and the seeds were larger and thicker than those of their diploids. These tetraploid breeding results will be useful for breeding new seedless watermelon cultivars.

• Journal of KSNVE
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• v.8 no.6
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• pp.1144-1149
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• 1998

A two degree-of-freedom model of suspended cables is studied for forced resonant response. The method of averaging is used to obtain first-order approximations to the response of the system. A bifurcation analysis of the averaged system is performed in the case of 2-to-1 internal resonance. Nonlinear coupled-mode motions are found to bifurcate from single-mode responses and further bifurcate to limit cycle motions via Hopf bifurcations. The limit cycle solutions undergo period doubling bifurcations to chaos.