• Journal of Applied Biological Chemistry
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• v.48 no.4
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• pp.183-186
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• 2005

Arabidopsis AHL gene encodes a 3'(2')-phosphoadenosine 5'-phosphate (PAP)-specific phosphatase that plays a role in the sulfate activation pathway. We complemented E. coli cysQ mutant defective in cysteine biosynthesis with the AHL gene. AHL cDNA was cloned into the prokaryotic expression vector pKK388-1 and transformed into the bacterial mutant. Since cysQ mutant is a leaky cysteine auxotroph only under aerobic conditions, the bacteria were grown in liquid media with vigorous shaking to provide more aeration. In cysteine-free medium, cysQ mutant and the mutant harboring empty vector did not grow well, whereas cells harboring AHL cDNA exhibited significantly improved growth with doubling time of approximately 3 h. cysQ is known to encode a 3'(2'),5'-diphosphonucleoside 3'(2')-phosphohydrolase (DPNPase). However, our data suggest that cysQ protein has PAP-specific phosphatase activity in addition to DPNPase activity. Microbial complementation procedure described in this paper is useful for structure-activity studies of PAP-specific phosphatases identified from microbes and plants.

• Current Research on Agriculture and Life Sciences
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• v.31 no.2
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• pp.88-93
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• 2013

The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 $kg/ha^-$ ($N-P_2O_5-K_2O$, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15.

• Current Research on Agriculture and Life Sciences
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• v.31 no.2
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• pp.75-82
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• 2013

Haploids are plants with a gametophytic number of chromosomes in their sporophytes. Androgenesis occurs from asymmetric division of pollen grains into generative cells and vegetative cells, followed by re-entry of the vegetative cell during S-phase, which causes microspores progress into G2/M transition in culture. One of the most interesting features of haploids is the possibility to produce doubled haploid (DH) individuals. Doubled haploidy is extremely useful to plant breeders because it enables shortened breeding periods and efficiency in selection of useful recessive agronomic traits. Doubled-haploid technology is not only applicable to breeding, but also to transformation programs of desired genes. In addition to practical breeding programs, DH lines provide useful materials of fundamental genetics including exploitation of QTLs and genes conferred with various agronomic traits by establishing DH populations. This paper provides historical overviews on androgenesis and describes several mechanisms associated with pollen embryogenesis, including mode of actions in pollen embryogenesis, mechanisms of chromosome doubling and factors affecting androgenesis. We also discuss recent progress in application of haploids to breeding, genes associated with in vitro response and drawbacks to anther culture for application of doubled haploids in crop breeding.

• Journal of the Korean Magnetic Resonance Society
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• v.15 no.2
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• pp.115-127
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• 2011

A newly developed cost-effective approach to prepare $^{15}N/^{13}C$-labeled protein for NMR studies is presented. This method has been successfully applied to isotopically labeling of PTK6 SH2 domain and MTH 1880 protein. The production method generates cell density using a growing media containing $^{15}NH_4Cl$, $^{12}C_6$-D-glucose. Following a doubling time period for unlabeled metabolite exhaustion and then addition $^{13}C_6$-D-glucose into a M9 growing media, the cells are induced. Our results demonstrate that in order to get full incorporation of $^{13}C$, the isotopes are not totally required during the initial growth phase before induction. The addition of small amounts of $^{13}C_6$-D-glucose to the induction phase is sufficient to obtain more than 95% incorporation of isotopes into the protein. Our optimized protocol is two-thirds less costly than the classical method using $^{13}C$ isotope during the entire growth phase.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.316.3-317
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• 2002

In previous reports, we exhibited that acteoside showed significant cytotoxicity against various cancer cells. In this study we investigated that acteoside is capable of inducing differentiation in HL -60 human leukemia cell line. After being treated with acteoside, the growth curve was decreased remakably in a dose- and time-dependent manner, and cell doubling time was delayed. Exposure of cells to 20 $\mu\textrm{g}$/m$\ell$ acteoside induced differentiation of HL-60 cells to monocyte/macrophage-like cells by cell surface antigen expression. The percentage of NBT reducing activity was increased in a time-dependent manner. In addition. the protein lever of p21 and p16 increased and ppRb decreased in western biot analysis. Theas results suggest that acleoside possess the activity of inducing differentiation in HL-60 cells.

• Progress in Superconductivity
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• v.10 no.2
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• pp.74-78
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• 2009

Inter-grain nanobridges of the $MgB_2$ superconductor have been fabricated by focused-ion-beam(FIB) and their electrical transport properties were studied. The $MgB_2$ film was prepatterned into microbridges by a standard argon ion milling technique and then FIB-patterned into 100 nm$\times$100 nm bridges. Current-voltage characteristics showed a strong flux-flow type behavior at all temperatures with a trait of Josephson coupling near $T_c$. At low temperatures, the curves showed a two-step resistance-doubled transition with occasional hysteresis. The resistance-doubling transition is believed to be due to a two-channel flux-flow effect. The temperature-dependent critical current data showed $I_c(T){\propto}(1-T/T_c)^2$ near $T_c$, same as a normal barrier junction, and $I_c(T){\propto}(1-T/T_c)^{1.2}$ at low temperatures, similar to that of a film.

• Journal of the Optical Society of Korea
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• v.15 no.3
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• pp.216-221
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• 2011

A diffraction grating is a highly symmetric optical element with a physical structure that is invariant under translational spatial movements. The translational symmetry is reflected in the fields that are diffracted from the grating. Here, we introduce a plane-parallel mirror pair onto the grating, which translates the fields through double reflections, and we describe a method of exploiting the symmetry to enhance the spectral resolution of a diffraction grating beyond the limit that is set by the number of grooves. The mirror pair creates another virtual grating beside the original one, effectively doubling the number of grooves. Addition of more mirror pairs can further increase the effective number of grooves despite the increased complexity and difficulty of experimental implementation. We experimentally demonstrate the spectral linewidth reduction by a factor of four in a neon fluorescence spectrum. Even though the geometrical restriction on the mirror deployment limits our method to a certain range of the whole spectrum, as a practical application example, a bulky spectrometer that is nearly empty inside can be made compact without sacrificing the resolution.

• KSBB Journal
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• v.25 no.2
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• pp.193-198
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• 2010

Cell cultures of Taxus wallichiana Zucc. were made to enhance the production of anticancer agent paclitaxel. In suspension cultures, the maximum cell growth rate in exponential growth phase was 0.14 $day^{-1}$ which was correlated to 4.96 days of cell doubling time. The production of paclitaxel was non-growth associated. The paclitaxel production was started after the exponential growth phase and increased to declined phase where the maximum concentration was observed. Various elicitors were tested to enhance the production of paclitaxel. The combination of two elicitors of jasmonic acid and cellulase increased the production of paclitaxel 1.8 and 3.1 times compared to paclitaxel production by individual elicitor respectively.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.41 no.5
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• pp.135-141
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• 2004

Image interpolation is an image enlargement method that calculates an empty pixel value using the information of given pixel values. Since a natural image is composed of various spatial frequency components, it is difficult for one method to interpolate pixels with various spatial frequencies. In this paper, we propose an image interpolation method using multiple neural networks with spatial frequency characteristic. Input image is segmented according to spatial frequency by local variance, and each segmented image is interpolated using neural network established for spatial frequency band. The proposed method is applied to line doubling that becomes an important part in image interpolation because of deinterlacing. In simulation the proposed algorithm shows the improved PSNR result compared with conventional algorithms and method using single neural network.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.6
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• pp.868-875
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• 2001

The objective of this study is to investigate the average droplet size distributions of a GDI spray by simultaneous fluorescence/scattering image technique. GDI engine is recently very popular because of high engine efficiency and low emissions. However, the injectors must have good spray characteristics because the fuel is directly injected into the cylinder. The fuel mixtures used in this study were 2% of fluorobenzene, 9% of DEMA(diethyl-methyl-amine) and 89% of hexane by volume. The system for obtaining 2-D fluorescence/scattering images of fuel spray was constituted of a laser sheet, a doubling prism, optical filters, and an ICCD camera. Using the ratio of the fluorescence to the scattering intensities, SMD distributions were obtained. SMD measured by the technique was compared with that obtained by PDA. It was found that average droplet size was bigger at spray center in the early stage of injection and at the outer periphery of the spray in the late stage of injection.