• Title/Summary/Keyword: Double-processing technique

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Applicability Evaluation of Male-Specific Coliphage-Based Detection Methods for Microbial Contamination Tracking

  • Kim, Gyungcheon;Park, Gwoncheol;Kang, Seohyun;Lee, Sanghee;Park, Jiyoung;Ha, Jina;Park, Kunbawui;Kang, Minseok;Cho, Min;Shin, Hakdong
    • Journal of Microbiology and Biotechnology
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    • v.31 no.12
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    • pp.1709-1715
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    • 2021
  • Outbreaks of food poisoning due to the consumption of norovirus-contaminated shellfish continue to occur. Male-specific (F+) coliphage has been suggested as an indicator of viral species due to the association with animal and human wastes. Here, we compared two methods, the double agar overlay and the quantitative real-time PCR (RT-PCR)-based method, for evaluating the applicability of F+ coliphage-based detection technique in microbial contamination tracking of shellfish samples. The RT-PCR-based method showed 1.6-39 times higher coliphage PFU values from spiked shellfish samples, in relation to the double agar overlay method. These differences indicated that the RT-PCR-based technique can detect both intact viruses and non-particle-protected viral DNA/RNA, suggesting that the RT-PCR based method could be a more efficient tool for tracking microbial contamination in shellfish. However, the virome information on F+ coliphage-contaminated oyster samples revealed that the high specificity of the RT-PCR- based method has a limitation in microbial contamination tracking due to the genomic diversity of F+ coliphages. Further research on the development of appropriate primer sets for microbial contamination tracking is therefore necessary. This study provides preliminary insight that should be examined in the search for suitable microbial contamination tracking methods to control the sanitation of shellfish and related seawater.

Prosthetic rehabilitation by double-processing technique for edentulous patient with soft palate defect after maxillectomy: A case report (연구개를 포함한 상악골 절제술을 받은 완전 무치악 환자에서 이중 온성법으로 제작한 구개 폐색장치를 통한 보철수복: 증례 보고)

  • Park, Jin-Yong;Wang, Yuan-Kun;Song, Kwang-Yeob;Park, Ju-Mi;Lee, Jung-Jin
    • The Journal of Korean Academy of Prosthodontics
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    • v.57 no.4
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    • pp.356-363
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    • 2019
  • A patient who went through maxillectomy can have soft palate defects including oronasal fistulas and suffer from dysphagia and dysarthria due to velopharyngeal insufficiency. This defect causes the food to enter nasal cavity and creates hypernasal sound which debilitates a quality of life. An obturator can rehabilitate the substantial oral tissue defects. The maxillary obturator separates the nasopharynx from the oropharynx during speech and deglutition by closing of the defect. For edentulous obturator patient, it is difficult to obtain proper retention due to reduced peripheral sealing. Therefore, the contours of the defects must be used to maximize the retention, stability, and support. Hollow type obturator can improve physiologic function by reducing weight than the traditional obturator. This case report describes a patient with hemi-maxillectomy who recovers mastication, speech, deglutition, and appearance with a maxillary obturator using physiological border molding of the velopharyngeal area and double-processing method.

A Design of DA_UDC(Double Authentication User.Device.Cross) Module using OTA(One Time Authentication) Key in Home Network Environment (홈 네트워크 환경에서 OTA(One Time Authentication)키를 이용한 DA_UDC(Double Authentication User.Device.Cross) 모듈 설계)

  • Jeong, Eun-Hee;Lee, Byung-Kwan
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.4B
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    • pp.419-427
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    • 2009
  • This paper propose DA-UDC(Double Authentication User, Device, Cross) Module which solves the cost problem and the appropriation of Certificate using User Authentication, Device Authentication and Cross Authentication with OTA(One Time Authentication) Key, and which is designed not to subscribe to the service of Home network business. Home Server transmits its public key which is needed to create OTA to the user which passed the first step of authentication which verifies User ID, Device ID and Session Key. And it performs the second step of authentication process which verifies the OTA key created by a user. Whenever the OTA key of DA-UDC module is generated, the key is designed to be changed. Therefore, DA-UDC Module prevents the exposure of User and Device ID by performing the two steps of authentication and enhances the authentication security of Home Network from malicious user with OTA key. Also, DA-UDC Module is faster than the existing authentication system in processing speed because it performs authentication calculation only once. Though DA-UDC Module increases data traffic slightly because of the extra authentication key, it enhances the security more than the existing technique.

Sensorless Control of a PMSM at Low Speeds using High Frequency Voltage Injection

  • Yoon Seok-Chae;Kim Jang-Mok
    • Journal of Power Electronics
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    • v.5 no.1
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    • pp.11-19
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    • 2005
  • This paper describes the two control techniques to perform the sensorless vector control of a PMSM by injecting the high frequency voltage to the stator terminal. The first technique is the estimation algorithm of the initial rotor position. A PMSM possesses the saliency which produces the ellipse of the stator current when the high frequency voltage is injected into the motor terminal. The major axis angle of the current ellipse gives the rotor position information at a standstill. The second control technique is a sensorless control algorithm that injects the high frequency voltage to the stator terminal in order to estimate the rotor position and speed. The rotor position and speed for sensorless vector control is calculated by appropriate signal processing to extract the position information from the stator current at low speeds or standstill. The proposed sensorless algorithm using the double-band hysteresis controller exhibits excellent reference tracking and increased robustness. Experimental results are presented to verify the feasibility of the proposed control schemes. Speed, position estimation and vector control were carried out on the floating point processor TMS320VC33.

Measurement Techniques on Unsteady Flow at Impeller Exit (임펠러 출구에서의 비정상 유동 측정 기법)

  • Shin, You-Hwan;Kim, Kwang-Ho
    • 유체기계공업학회:학술대회논문집
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    • 1998.12a
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    • pp.123-128
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    • 1998
  • This study presents the measurement techniques on the periodic fluctuating flow such as the discharge flow of a centrifugal impeller in unstable operating region. During rotating stall, the flow at the exit of a centrifugal compressor impeller fluctuates periodically with lower frequency than that of the blade passing. To observe the blade-to-blade flow characteristics during rotating stall, the phases of all the sampled data sets should be adjusted to those of the reference signals with two processes, in these processes, DPLEAT (Double Phase-Locked Ensemble Averaging Technique) can be used. From these measurement and data processing techniques, the characteristics not only on the blade-to-blade flow with high frequency, but also on the periodic rotating stall flow with low frequency at the centrifugal impeller exit can be clearly observed.

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Microwave Hydrothermal Sythesis of PbTiO$_3$ and PMN Ceramic Powders (마이크로파 수열법에 의한 PbTiO$_3$ PMN 세라믹분말의 합성)

  • Bai, Kang
    • Journal of the Korean Ceramic Society
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    • v.35 no.5
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    • pp.465-471
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    • 1998
  • Lead titanate(PT) and lead magnesium niobate(PMN) ceramic powders were prepared by microwave hy-drothermal method using teflon bomb. Raw materials were Pb(NO3)2 and TiO2 for lead titanate and Pb(NO3)2 Nb2O5 and Mg(NO)3.6H2O for PMN with NaOH as mineralizer in both cases. in lead titanate synthsis rate of microwave hydrothermal method was faster three times than one f conventional hydrothermal methods In lead magnesium niobate synthsis the mixture of perovskite and pyrochlore phases was obtained by single step technique and the PMN was not obtained by double step technique due to low temperature limitation of teflon bomb.

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Sensorless control of PMSM in low speed range using high frequency voltage injection (전압주입 방식을 이용한 PMSM 센서리스 제어에 관한 연구)

  • Yoon Seok-chae;Kim Jang-mok
    • Proceedings of the KIPE Conference
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    • 2003.11a
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    • pp.119-122
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    • 2003
  • This paper describes the sensorless technique for the surface-mounted permanent-magent synchronous motor(SPMSM or PMSM) drive based on magnetic saliency. The control technique is a sensorless control algorithm that injects the high frequency voltage to the stator terminal in order to estimate the rotor position and speed. The rotor position and speed for sensorless vector control is achieved by appropriate signal processing to extract the position information from the stator current in the low speed range including zero speed. Proposed sensorless algorithm using the double-band hysteresis controller and initial rotor position detection exhibits excellent reference tracking and increased robustness. Experimental results are presented to verify the feasibility of the proposed control schemes.

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Measurement Techniques on Unsteady Flow at Impeller Exit (임펠러 출구에서의 비정상 유동 측정 기법)

  • Shin, You-Hwan;Kim, Kwang-Ho
    • The KSFM Journal of Fluid Machinery
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    • v.2 no.1 s.2
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    • pp.81-87
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    • 1999
  • This study presents the measurement techniques on the periodic fluctuating flow such as the discharge flow of a centrifugal impeller in an unstable operating region. During rotating stall, the flow at the exit of a centrifugal compressor impeller fluctuates periodically with a lower frequency than that of the blade passing. To observe the blade-to-blade flow characteristics during the rotating stall, the phases of all the sampled data sets should be adjusted to those of the reference signals with two processes, in these processes, DPLEAT (Double Phase-Locked Ensemble Averaging Technique) can be used. From these measurements and data processing techniques, the characteristics illustrated a blade-to-blade flow with high frequency, but also a periodic rotating stall flow with a low frequency at the centrifugal impeller exit which was clearly observed.

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Folding fan Production Incorporated into Engineering Education - "Monodzukuri" Learning from Traditional Technique in Japan -

  • ABE, Fujiko;OHBUCHI, Yoshifumi;SAKAMOTO, Hidetoshi
    • Journal of Engineering Education Research
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    • v.22 no.5
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    • pp.49-55
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    • 2019
  • Folded structure is widely applied in various engineering fields. Many of the Japanese folding fans in the Edo era (1603-1868) have been successfully blended with the processing technology of "natural materials" that is the origin of Japan's "Monodzukuri" (craftsmanship) and its application "artistic originality". The charm of a fan lies in the diversity of stereoscopic expression not born in plane representation. For example, the effects of folds, the expression of the front and back sides flowing from the front to the back by double-sided description, and the two-layer effect of raising the backside from the surface using the permeability of Japanese paper, the calculated depiction are also seen. Moreover, by handling the fan, it also produced an illusion effect which skillfully calculated the change due to movement of the viewpoint. Students experience the natural materials such as Japanese paper, bamboo and starch paste, which are the materials of paint and fan at the time, and processing method, and know the difference with the current one. This study is to verify the effectiveness of engineering education which gains experience by making concrete fans and to understand deeply this traditional technology with the artistry of a Japanese fan at the same time. And we can learn from the characteristics of the fan to Japan's history and culture.

Impact of Tropospheric Delays on the GPS Positioning with Double-difference Observables (대류권 지연이 이중차분법을 이용한 GPS 측위에 미치는 영향)

  • Hong, Chang-Ki
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.31 no.5
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    • pp.421-427
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    • 2013
  • In general, it can be assumed that the tropospheric effect are removed through double-differencing technique in short-baseline GPS data processing. This means that the high-accuracy positioning can be obtained because various error sources can be eliminated and the number of unknown can be decreased in the adjustment computation procedure. As a consequence, short-baseline data processing is widely used in the fields such as deformation monitoring which require precise positioning. However, short-baseline data processing is limited to achieve high positioning accuracy when the height difference between the reference and the rover station is significant. In this study, the effects of tropospheric delays on the determination of short-baseline is analyzed, which depends on the orientation of baseline. The GPS measurements which include tropospheric effect and measurement noises are generated by simulation, and then rover coordinates are computed by short-baseline data processing technique. The residuals of rover coordinates are analyzed to interpret the tropospheric effect on the positioning. The results show that the magnitudes of the biases in the coordinate residuals increase as the baseline length gets longer. The increasing rate is computed as 0.07cm per meter in baseline length. Therefore, the tropospheric effects should be carefully considered in short-baseline data processing when the significant height difference between the reference and rover is observed.