• Bulletin of the Korean Chemical Society
• /
• v.34 no.3
• /
• pp.823-826
• /
• 2013

A low-${\varepsilon}$ solid-state NMR(Nuclear Magnetic Resonance) probe was developed for the spectroscopic analysis of two-dimensional $^{15}N-^1H$ heteronuclear dipolar coupling in dilute membrane proteins oriented in hydrated and dielectrically lossy lipid environments. The system employed a 800 MHz narrow-bore magnet. A solenoid coil strip shield was used to reduce deleterious RF sample heating by minimizing the conservative electric fields generated by the double-tuned resonator at high magnetic fields. The probe's design, construction, and performance in solid-state NMR experiments at high magnetic fields are described here. Such high-resolution solid-state NMR spectroscopic analysis of static oriented samples in hydrated phospholipid bilayers or bicelles could aid the structural analysis of dilute biological membrane proteins.

• Journal of the Korea Institute of Building Construction
• /
• v.13 no.6
• /
• pp.513-521
• /
• 2013

This study aimed to examine whether heat curing methods of concrete subjected to $-10^{\circ}C$ could be effective by varying the combination of heating cable and surface heat insulations. Three different concrete specimens incorporating 30% fly ash with 50% W/B were fabricated to simulate wall, column and slab members with dimensions of $1600{\times}800{\times}200$ mm for slab, $800{\times}600{\times}200$ mm for wall and $800{\times}800{\times}800$ mm for column. For heat curing combinations, Type-1 specimens applied PE film for slab, plywood for wall and column curing. Type-2 specimens applied double layer bubble sheet (2LB) and heating coil for slab, and 50 mm styrofoam for wall and column curing. Type-3 specimen applied 2LB for slab, electrical heating mat for wall and column inside heating enclosure. The test results revealed that the temperature of Type 1 specimen dropped below $0^{\circ}C$ beginning at 48 hours after placement due to its poor heat insulating capability. Type 2 and 3 specimens maintained a temperature of around $5{\sim}10^{\circ}C$ after placement due to favorable heat insulating and thermal resistance.

• Proceedings of the KIEE Conference
• /
• summer
• /
• pp.584-586
• /
• 2004

This paper deals with the development of on-line fault and lightning stroke section locating system for branched overhead transmission line. The section locating algorithm of this system i3 by analyzing the distribution pattern of current flowing through the overhead ground wire. It composes of three parts; current sensors, local remote terminal unit(RTU) and analysis program at surveillance tenter. Double Rogowski coil sensor having integrating amplifier was designed as current sensor. In order for current pattern analysis, the transmitted waves from each sensor wert synchronized by GPS tim c clock in RTU. While, lightning stroke location are judged only by polarity information of lightning currents. This design has a benefit in simplicity of signal processing unit of RTU.

• BMB Reports
• /
• v.38 no.5
• /
• pp.591-594
• /
• 2005

One of the small proteins from Helicobacter pylori, HP1242, was investigated by the solution nuclear magnetic resonance (NMR) spectroscopy. HP1242 is known as a 76-residue conserved hypothetical protein and its function cannot be identified based on sequence homology. Here, the results of the backbone $^1H$, $^{15}N$, and $^{13}C$ resonance assignments of the HP1242 are reported using double- and triple-resonance techniques. About 95% of all of the $^1HN$, $^{15}N$, $^{13}CO$, $^{13}C{\alpha}$, and $^{13}C{\beta}$ resonances that cover 75 non- Proline residues of the 76 residues are clarified through sequential- and specific- assignments. In addition, three helical regions were clearly identified on the basis of the resonance assignments.

• Proceedings of the KSME Conference
• /
• 2002.08a
• /
• pp.361-364
• /
• 2002

An electromagnetic flowmeter(EMF) was developed and its characteristics were compared with a commercial EMF. The measuring tube of the EMF was basically designed as 100 m diameter. A signal processing circuit was also developed for generating the magnetic field and converting the flow signal to flowrate and flow quantity. To obtain a more stable and reliable flow signal, the double magnetizing frequency was adopted for magnetizing the coil of the EMF For the characterization of EU, the uncertainty of calibrator was estimated within ${\pm}0.5{\%}$, the method of estimation was in accordance with the ISO 17025 recommendation. It was found that the flow signals between the electrodes were about ${\pm}60\;-\;{\pm}300{\mu}V$, which were sufficient for the discrimination of the flowmeter and protecting the noise. The test result against the calibrator, showed the good linearity in the range of $3 m^{3}/h\;and\;70m^{3}/h$. A commercialized design of the EMF will be technically more competitive in domestic and foreign market.

• Progress in Superconductivity
• /
• v.2 no.1
• /
• pp.20-26
• /
• 2000

A 40-channel superconducting quantum interference device (SQUID) system was constructed for measuring neuromagnetic fields. Main features of the system are the use of double relaxation oscillation SQUIDs (DROSs), and planar gradiometers measuring magnetic field components tangential to the head surface. The DROSs with high flux-to-voltage transfers enabled direct readout of the SQUID output by room-temperature dc preamplifiers and simple flux-locked loop circuits could be used for SQUID operation. The pickup coil is an integrated first-order planar gradiometer with a baseline of 40 mm. Average noise level of the 40 channels is around 1.2 $fT/cm/{\surd}Hz$ at 100 Hz, corresponding to a field noise of 5 $fT/{\surd}Hz$, operated inside a magnetically shielded room. The SQUID insert was designed to have low thermal load, minimizing the loss of liquid helium. The constructed system was applied to measure auditory-evoked neuromagnetic fields.

• Microbiology and Biotechnology Letters
• /
• v.13 no.3
• /
• pp.297-302
• /
• 1985

The 7.1 Mdal Xbaf fragment of Bacillus pasteurii ATCC 11859 containing gene for urease was inserted into the Xbal site of bifunctional plasmid pGR71, and its urease gene was cloned and expressed in E. coil RRI. But the cloned gene was not expressed in Bacillus subtilis BR151 in consequence of deletion of inserted DNA fragment. The recombinant plasmid thus formed was named pGU66. The restriction map of the plasmid pGU66 was determined, and the size of the plasmid was estimated to be 12.6 Mdal by double digestion of restriction enzymes of the plasmid. The urease of the cloned strain was accumulated in periplasmic space and very similiar to that of donor strains in their enzymatic properties.

• Journal of the Korean Magnetics Society
• /
• v.2 no.1
• /
• pp.50-55
• /
• 1992

Numerical method for analyzing the reading process in magnetic recording system is described. Hysteresis characteristics must be included in numerical analysis not only recording process but also reading process because of hysteretic behavior of recording media. Algorithm which is consisted of finite clement method for numerical analysis and Preisach model considered to be more appropriate method than Stoner-Wohlfarth model with spin curling mode for describing hysteresis characteristics is constructed. Equations to calculate waveform of reproduced voltage is proposed. Then, this is applied to perpendicular magnetic recording systems with pole type head and double layer media. Waveform of magnetic flux density and reproduced voltage induced in head coil is obtained. If the recording current increase in recording process, magnitude of reproduced voltage in reproducing process saturates. From this saturation curve, value of current which produce maximum voltage can be obtained.

• Proceedings of the KIEE Conference
• /
• 1994.07b
• /
• pp.1589-1592
• /
• 1994

We developed the ion plating system, consisted of the Facing Target Magnetron Sputtering System and the r.f, electrode of the coil type, which was available to control the reactive and the adhesion between thin film and substrate, and studied about the discharge characteristics and the optimum condition in order to form the high quality thin film. The characteristics of discharge and plasma was measured as Double Probe and Electrostatic Retarding Grid Analyzer. The incident ion energy on the substrate was increased as the increasing r.f power, bias voltage. By the r.f electrode, the ionization rate of the sputtered particles was about 75%, and the mean incident ion energy depend on the value which was difference between the plasma potential and biased substrate potential.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2008.06a
• /
• pp.289-289
• /
• 2008

We investigated the operational characteristics of the separated three-phase flux-lock type superconducting fault current limiter (SFCL). The single-phase lock type SFCL consist of two coils, which are wound in parallel through an iron core. The high-$T_c$ superconducting(HSTC) thin film connected in series with secondary coil. The separated three-phase flux-lock type SFCL consist of three single-phase flux-lock type SFCL. In a normal condition, the SFCL is not operate. When a fault occurs, the current of a HSTC thin film exceeds its critical current by fault current, the resistance of the HSTC thin film generated. Therefore fault current was limited by SFCL. The separated three-phase flux-lock type SFCL are operated in fault condition such as the the single line-to-ground fault, the double line-to-ground fault and the triple line-to-ground fault. The experimental results, the SFCL operational characteristics was dependent on fault condition.