• Proceedings of the Optical Society of Korea Conference
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• 2009.02a
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• pp.133-134
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• 2009

Cold temperature development (CTD) of electron beam (EB) patterned resists and subsequent dry etching were investigated for fabrication of nano-patterned Niobium (Nb). Bulky Nb fims on GaAs substrates were deposited with EB evaporation. Line patterns on Nb cathode were fabricated by EB patterning and reactive ion etching (RIE). Size deviations of nano-sized line patterns from CAD designed patterns are dependent on the EB total exposure, but it can be improved by CTD of EB-exposed resist. Line patterns of 10 to 300 nm widths of EB-exposed resist patterns were drawn under various exposure conditions of $0.2{\mu}s$/dot (total 240,000 dot) with a constant current (50 pA). Compared with room temperature development (RTD), the CTD improves pattern resolution due to the suppression of backscattering effect. RIE with $CF_4$ was performed for formation of several nano-sized line patterns on Nb. Each EB-resist patterned samples with RTDs and CTDs were etched with two different $CF_4$ gas pressures of 5 Pa. Nb etching rate increases while GaAs (or ZEP) etching rate decreases as the chamber pressure increases. This different dependent of the etching rate on the $CF_4$ pressure between Nb and GaAs (or ZEP) has a significant meaning because selective etching of nano-sized Nb line patterns is possible without etching of the underlying active layer.

• Journal of the Korean Ceramic Society
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• v.41 no.10 s.269
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• pp.788-791
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• 2004

We have studied the fabrication of silver micro lines using the silver nano sol on ITO substrate by an ink-jet method. The average particles size of $10wt\%$ silver nano sol synthesized with polyelectrolytes was smaller than 10 nm. The pattern formation of silver nano sol on the substrate closely related with the contact angle of the silver nano sol. The dot shaped of silver nano sol on bare ITO substrate was formed due to the high contact angle of silver nano sol. When ITO substrates was treated with 100 ppm polyethylenimine for silver nano sol patterning, fine silver micro lines of $60{\sim}100{\mu}m$ width was fabricated by ink-jet method.

• Journal of Periodontal and Implant Science
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• v.47 no.3
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• pp.174-181
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• 2017

Purpose: Epitope spreading is a phenomenon in which distinct subdominant epitopes become major targets of the immune response. Heat shock protein (HSP) 60 from Porphyromonas gingivalis (PgHSP60) and peptide 19 from PgHSP60 (Pep19) are immunodominant epitopes in autoimmune disease patients, including those with periodontitis. It remains unclear whether Pep19 is a dominant epitope in subjects without periodontitis or autoimmune disease. The purpose of this study was to determine the epitope spreading pattern and verify Pep19 as an immunodominant epitope in healthy teenagers using dot immunoblot analysis. The patterns of epitope spreading in age-matched patients with type 1 diabetes mellitus (type 1 DM) and healthy 20- to 29-year old subjects were compared with those of healthy teenagers. Methods: Peptide from PgHSP60, Mycobacterium tuberculosis HSP60 (MtHSP60), and Chlamydia pneumoniae HSP60 (CpHSP60) was synthesized for comparative recognition by sera from healthy subjects and patients with autoimmune disease (type 1 DM). Dot immunoblot analysis against a panel of peptides of PgHSP60 and human HSP60 (HuHSP60) was performed to identify epitope spreading, and a densitometric image analysis was conducted. Results: Of the peptide from PgHSP60, MtHSP60, and CpHSP60, PgHSP60 was the predominant epitope and was most consistently recognized by the serum samples of healthy teenagers. Most sera from healthy subjects and patients with type 1 DM reacted more strongly with PgHSP60 and Pep19 than the other peptides. The relative intensity of antibody reactivity to Pep19 was higher in the type 1 DM group than in the healthy groups. Conclusions: Pep19 is an immunodominant epitope, not only in autoimmune disease patients, but also in healthy young subjects, as evidenced by their robust immunoreactivity. This result suggests that the Pep19-specific immune response may be an initiator that triggers autoimmune diseases.

• Toxicological Research
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• v.25 no.1
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• pp.35-40
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• 2009

TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and related halogenated aromatic hydrocarbons elicit a diverse spectrum of biochemical and toxic responses in laboratory animals and mammalian cells in culture. Toxicity and carcinogenicity of TCDD is well established but the molecular mechanism is still poorly understood. Here, we found the noble responsive genes to TCDD using the differential display analysis. Treatment of HepG2 cells with TCDD showed a significantly different mRNA expression pattern from the untreated cells in differential display analysis. The differentially displayed bands were isolated and used as probes in dot blot and Northern blot analyses. Of thirty-five isolated differentially displayed bands, only two bands were confirmed as positive in dot blot and Northern blot analyses. The nucleotides sequences of these clones were analyzed and the search of Genebank database revealed that one clone is highly homologous with RanBP2 (Ras-related nuclear protein binding protein2; 92%) and the other is an unknown gene. RanBP2 is a nucleoporin with SUMO E3 ligase activity that functions in both nucleocytoplasmic transport and mitosis and its role as a novel tumor suppressor has been recently proposed. Thus, these results may suggest the clue elucidating the toxic mechanism of TCDD through RanBP2.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.05a
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• pp.520-523
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• 2002

The o]d technique of sandblasting which has been used for paint or scale removing, deburring, and glass decorating has recently been developed into a powder blasting technique for brittle materials, capable of producing micro structures larger than $100\mu\textrm{m}$. In this study, we investigated the effect of the size of abrasive and the stand-off distance on the machinability of SUS 304. The varying parameters were the impact angle $90^{\circ}$, scanning times of nozzle 10 and the different nozzle height between 70mm and 140mm. The diameter of dot pattern were 0.2mm. The powder were alumina sharp particles, WA #600, #800 and #2000. The blasting pressure of powder at 0.3 Mpa.

• Journal of Engineering Education Research
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• v.18 no.6
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• pp.52-56
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• 2015

Usually, students write on a paper using writing materials and submit it to teachers when they do a homework or take an exam. However, there are temporal and spacial constraints because teachers and students should be in the same place at the time in this system. In addition, it is hard for teachers to maintain numerous papers from students. Applying a digital pen produced by Pen Generations Corporation to education system, we developed a smart education system transmitting image data from written papers using digital pen. As a result, teachers and students can use this system without any constraints. In addition, teachers are able to maintain their papers from students more effectively.

• Journal of Distribution Research
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• v.9 no.1
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• pp.1-24
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• 2004

As the dot-com companies realize the importance of the traditional business operations such as product distribution and sourcing, the task of balancing business strategy and operations between electronic commerce and"bricks St mortar"channels is an ongoing challenge. However, there are no generic recipes for formulating business strategy that optimally bridges the physical and virtual operations. In order to provide some guidelines, this study examines the big 3 retailers in Korea, and their"clicks ＆ mortar"strategy, assessing how the activities of each channel are integrated to achieve the synergy effect. This study analyzes the differences in the pattern of integration among the three retailers and traces the causes of those differences. It also figures out the relationship between the degree of integration and performance variables such as growth and cost. Finally. it defines the essential constraints on the process of integration along with managerial implications.

• Journal of Welding and Joining
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• v.19 no.1
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• pp.75-81
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• 2001

The metallurgical study of microconstituents in transient liquid phase bonded joints of Ni-base single crystal superalloys, CMSX-2 and CMSX-4 was investigated employing MBF-80 insert metal. TLP bonding of specimens was carried out at 1,373~1,523K for 0~19.6ks in vacuum. Three types of microconstituents ; needle-like constituent, dot-like constituent and abnormal shape constituent were formed in the bonded interlayer during TLP bonding operation. All these microconstituents were identified as boride. Microconstituents contain a large percentage of Cr in the early stage of bonding. As increasing the holding time, the amount of Cr was decreased and the amount of W, Co and Re were increased. From the analysis results of electron diffraction pattern by TEM, composition of elements in microconstituents were into MBlongrightarrowM$_{5}$B$_3$longrightarrowM$_2$B type with the increased in holding time. It can be explained by the fact that the relative amount of boron in microconstituents was decreased when the holding time was increased.d.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.38 no.3
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• pp.11-11
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• 2001

일반적 칼라 출력 장치는 제한된 범위의 색만을 재현 할 수 있다. 특히 프린터와 같이 색의 재현성이 부족한 장비에서는 하드웨어 특성에 따른 채색 면적이나 인쇄에 사용되는 용지의 특성에 따라 염료의 확산 등에 대해서도 고려해야 한다. 특히, 색을 프린트하기 위해서는 출력 과정에서 색의 선형적인 증가와 감소가 이루어져야 하며 또한 정확한 색의 모델링이 가능하여야 한다. 따라서 본 논문에서는 이를 위해 프린터 출력시 실제 채색 면적과 시각적 색감을 고려할 수 있는 디더링 방법을 제안한다. 제안된 방법은 실제 채색 면적을 고려한 패턴 데이터 베이스를 만들어 이를 디더링 패턴으로 이용하게 된다. 그리고 이 데이터 베이스에서 정확한 패턴을 선택하기 위해서는 인간시각의 색 인지력을 모델링한 대조 민감도 함수를 사용하게 된다. 따라서 본 논문에서는 저해상도의 칼라 출력장치에서도 고화질의 색을 재현할 수 있게 해준다.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1999.11a
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• pp.578-581
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• 1999

Dot pattern print methods composed of a diffusion film and two prism sheets, have been generally used for backlighting systems of LCDs. However, this methods require complex structures and show high power consumption and optical loss. To improve these disadvantages of conventional backlight units, light guides using highly scattering optical transmissions (HSOT) polymer as scatters, have been introduced. In this study we analyzed multiple scattering effect in light guide by means of Monte carlo simulation based on Mie scattering theory and ray tracing method. As a result it was revealed that scattering intensity depends on the size of scatters. On the other hands, it was shown that scattering efficiency depends on the wavelength of fluorescent lamp as well as the size of scatters.