• Title/Summary/Keyword: Dongjin 1

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Efficient Extraction of Bioethanol from Freshwater Cyanobacteria Using Supercritical Fluid Pretreatment

  • Pyo, Dongjin;Kim, Taemin;Yoo, Jisun
    • Bulletin of the Korean Chemical Society
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    • v.34 no.2
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    • pp.379-383
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    • 2013
  • For the production of ethanol from freshwater cyanobacteria, a new pretreatment method using supercritical fluid was introduced. In this study, it was found that the supercritical fluid could penetrate inside the cell wall and help to liberate starch from cyanobacterial cells which resulted in the increase of the efficiency of ethanol production. For Microcystis aeruginosa, supercritical fluid pretreatment increased the amount of ethanol produced from cyanobacteria from 1.53 g/L to 2.66 g/L. For Anabaena variabilis, the amount of ethanol was increased from 1.25 g/L to 2.28 g/L. With use of supercritical fluid pretreatment, the efficiency of the process to obtain higher ethanol yields from freshwater cyanobacteria was improved upto 80%. The optimum temperature and pressure conditions for supercritical fluid pretreatment were determined as the temperature of $40^{\circ}C$ and the pressure of 120 atm. This study demonstrates the feasibility of using supercritical fluid pretreatment for ethanol production using freshwater cyanobacteria.

Effects of Moisture Contents of Rough Rice and Storage Temperatures on Rice Grain Quality (정조 함수율 및 저장온도에 따른 쌀 품질 특성변화)

  • Choi, Yoon-Hee;Jeong, Eung-Gi;Choung, Jin-Il;Kim, Deog-Su;Kim, Sun-Lim;Kim, Jung-Tae;Lee, Choon-Gi;Son, Jong-Rok
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.12-20
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    • 2006
  • This study was carried out to investigate the relationship between each quality characteristics and changes of grain quality according to storage temperature and moisture contents of rough rice of Dongjin 1 and Siodongjinbyeo. Respiration rate of rough rice and fat acidity of brown rice were increased with moisture contents of rough rice and storage temperature. Germination rate of rough rice was the highest at $17{\sim}18%$ moisture contents of rough rice stored at low temperatures for 12 months. Germiation rate of the stored at low temperature after 16 months was up to 80% however the rate of $13{\sim}14%$ moisture contents of rough rice stored at room temperature was severely decreased to below the 10%. Gloss value of cooked rice was increased with moisture contents of rough rice and the lower storage temperature. Fat acidity of brown rice was lower in Sindongjinbyeo than Dongjin 1 at the lower storage temperature. Sensory score of cooked rice of Sindongjinbyeo which stored at low temperature for 16 months was better than Dongjin 1 and excllent at $17{\sim}18%$ moisture contents of rough rice. Correlations between sensory score of cooked rices and color b value of brown rice, gloss value of cooked rice and color L value of brown rice were significant 0.731**, 0.625*, and 0.615*, resectively, and were also positively significant correlated with break down, peak viscosity, pasting temperature, and fat acidity of brown rice -0.864**, -0.795**, -0.786**, and -0.779**, respectively.

On the Bacterivory of Ciliates in the Estuarine System of the Mankyung and Dongjin rivers. (만경.동진강 염하수에서 섬모충류에 의한 박테리아 섭식에 관하여)

  • SHIM, JAE HYUNG;PARK, SOO YOUNG;CHO, BYUNG CHEOL;YIH, WONHO
    • 한국해양학회지
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    • v.30 no.5
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    • pp.426-435
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    • 1995
  • In order to assess the significance of ciliate grazing on bacterial population in carbon flow of the estuarine pelagic ecosystem of the Mankyung and Dongjin river (MD estuary), abundance and biomass of ciliates and grazing rate on bacteria of small (<40 um) ciliates were measured. Saplings were carried out four times from October 1993 to March 1995 in the estuarine system. Ciliates smaller than 40 um occupied more than 49.5% of total ciliates abundance. Clearance rate of small ciliates ranged from 18.0 to 16.3 nl cell/SUP -1/ h/SUP -1/. As bacterial abundance increased, ciliate's clearance rate decreased, suggesting that bacterial abundance effects on ciliate's grazing rate. Ciliate grazing rate was equal to 0.1 to 12.2% of bacterial productivity, and the ratio of ciliate ingestion over bacterial production increased exponentially with the increase of bacterial abundance (r$^2$=0.62, p$\leq$0.001). It seems that the effect of ciliate grazing on bacteria would be small in coastal waters and large in more eutrophic waters of high bacterial abundance. Carbon supplied by ciliate grazing on bacteria was avg. 3.1% of carbon amount required for the ciliate maximum productivity. Thus, the ecological role of ciliate in microbial loop was probably more important as a final consumer than as a direct consumer of bacteria.

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Environments and distribution of Benthic Animals on the Mangyung-Dongjin Tidal Flat, West Coast of Korea (서해 만경.동진 조간대의 환경과 저서동물 분포)

  • 안순모;고철환
    • 한국해양학회지
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    • v.27 no.1
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    • pp.78-90
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    • 1992
  • The present study is aimed to investigate the relationship between the distribution of benthic macro fauna and environments in the Mangyung-dongjin tidal flat developed on the west coast of central korea. Samples were taken from 69 sites established on 8 transect lines running perpendicular to the coast. The 69 sites located in the range of -250 to 230 cm height from the mean sea level were categorized into 4 levels of exposure duration. The mean grain sizes of the surface sediments varied from 1.5 to $7.9{\varphi}$. Silty sands and sands were dominant sediment facies on the tidal flat. A total of 64 species of invertebrates was collected and the mean density was $1,335{\;}indiv{\cdot}\textrm{m}^2$. The dominant species was Laternula cf. limicola of Bivalvia (71% of total counts), followed by Umbonium thomasi of Gastropoda (22%), and Lingula anatina of Brachiopoda (2.6%). These 3 species comprised 95.5% of total individual number. The distribution of benthic animals was closely related with the duration of tidal exposure. Perinereis association was the characteristic for the most exposed area, and followed by Macrophthalmus and Bullacta-Mactra-Umbonium association along the degree of exposure duration.

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Assessment of Pollutant Loads for Water Enhancement in the Jeongeupcheon of Dongjin River (동진강 정읍천 유역 오염부하량 평가)

  • Lee, Kyeong-Bo;Kim, Jong-Cheon;Park, Ji-Hye;Lee, Deog-Bae;Kim, Jong-Gu
    • Korean Journal of Environmental Agriculture
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    • v.23 no.1
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    • pp.41-46
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    • 2004
  • The influence of pollutant loads on the water quality in Jeongeupcheon of Dongjin river was evaluated from Jan. 2002 to Dec. 2003 for two years. The range of pH in water was $6.87{\sim}7.53$. The EC level in upstream ranged from 83 to $95\;{\mu}s/cm$ with the highest value in autumn. The BOD level in upstream rang ed from 0.61 to 1.27 mg/L, which would be I grade according to water quality criteria by Ministry of Environment, but that in downstream was III grade. The average T-N level in midstream ranged from 6.10 to 10.84 mg/L which was the highest values throughout the stretch of the river. The average T-P levels ranged from. 0.41 to 0.98 mg/L Jeongeupcheon was suitable for the agricultural usage based upon one year analysis of river water quality. The effluent loads of BOD was high in midstream (J4) with 553 kg/day. The major sources of T-N loads were livestock, population, land use, and industry in order. The effluent loads of T-N was high in J4 by population and industry while that of T-N was high in J5 and J6 by livestock and land use. The delivered loads of T-N was high in downstream The delivered loads of T-P was low as compared with those of BOD and T-N. The delivery ratio of T-N ranged from 6 to 38%.

Heat Shock RNA 1, Known as a Eukaryotic Temperature-Sensing Noncoding RNA, Is of Bacterial Origin

  • Choi, Dongjin;Oh, Hye Ji;Goh, Chul Jun;Lee, Kangseok;Hahn, Yoonsoo
    • Journal of Microbiology and Biotechnology
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    • v.25 no.8
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    • pp.1234-1240
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    • 2015
  • Heat shock RNA 1 (HSR1) is described as a "eukaryotic heat-sensing noncoding RNA" that regulates heat shock response in human and other eukaryotic cells. Highly conserved HSR1 sequences have been identified from humans, hamsters, Drosophila, Caenorhabditis elegans, and Arabidopsis. In a previous study, however, it was suggested that HSR1 had originated from a bacterial genome. HSR1 showed no detectible nucleotide sequence similarity to any eukaryotic sequences but harbored a protein coding region that showed amino-acid sequence similarity to bacterial voltage-gated chloride channel proteins. The bacterial origin of HSR1 was not convincible because the nucleotide sequence similarity was marginal. In this study, we have found that a genomic contig sequence of Comamonas testosteroni strain JL14 contained a sequence virtually identical to that of HSR1, decisively confirming the bacterial origin of HSR1. Thus, HSR1 is an exogenous RNA, which can ectopically trigger heat shock response in eukaryotes. Therefore, it is no longer appropriate to cite HSR1 as a "eukaryotic functional noncoding RNA."

Investigation of Transformation Efficiency of Rice Using Agrobacterium tumefaciens and High Transformation of GPAT (glycerol-3-phosphate acyltransferase) Gene Relative to Chilling Tolerance (Agrobacterium tumefaciens를 이용한 벼의 형질전환 효율의 검토 및 내한성 관련 GPAT (glycerol-3-phosphate acyltransferase) 유전자의 형질전환)

  • Seo, Mi-Suk;Bae, Chang-Hyu;Choi, Dae-Ock;Rhim, Seong-Lyul;Seo, Suk-Chul;Song, Pill-Soon;Lee, Hyo-Yeon
    • Journal of Plant Biotechnology
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    • v.29 no.2
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    • pp.85-92
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    • 2002
  • This study has been focused on improving transformation efficiency of rice using Agrobacterium tumefaciens. We have demonstrated the effect of this system when the GPAT gene related to the cold-resistance was transferred by Agrobacterium tumefaciens in rice. Transformation conditions were modified using intron $\beta$-glucuronidase (GUS) expression as a reporter gene in the rice. In this study, mature seed-derived calli of rice (Oruza sativa L. cv. Dongjin) were pre-cultured for 3 days and then infected with Agrobacterium. When this infected calli were cultured in the dark for 10 days on co-cu]lure medium containing 50 mg/L of CaCl$_2$, 30 mg/L of acetosyringone, 2 mg/L of 2,4-D, 120 mg/L of betaine, high GUS expression was observed. In the present transformation system, the efficiency of transformation of GPAT gene was about 54%. Stable integration of GPAT gene into chromosomal DNA was proven by southern blot analysis of genomic DNA isolated from T$_{0}$ progenies. The progenies (T1 generation) derived from primary transformant of 5 lines were segregated with a 3 (resistant) : 1 (sensitive ratio) in medium containing hygromycin. This high frequency transformation system can be used as a useful tool in transformation of another monocotyledon.n.

A Water Budget Analysis with Inter-basin Water Transfer Taken Spatially into Considerations (외부공급을 공간적으로 고려한 물수지 분석)

  • Yoon, Yong Nam;Kim, Tae Guen
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.13 no.1
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    • pp.89-96
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    • 1993
  • The conventional water budget analysis methods for major water works do not take the spatial variation of available water resources into account. There has also been a tendency of over-estimation of water supply capacity when a significant inter-basin water transfer is involved. Therefore, a revised water budget analysis methodology is proposed which can take care of the spatial variation of the water resources available within the basin and with the inter-basin transferable water. For the purpose of present study, the Saemangeum project area is taken for the analysis, which includes both the Dongjin and the Mangyungs River basin, one of the regions with the most complex water utilization.

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