• Korean Journal of Breeding Science
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• v.42 no.4
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• pp.365-373
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• 2010

TILLING (Targeting Induced Local Lesions IN Genomes) is broadly regarded as an excellent methodology for reverse genetics applications. Approximately 15,000 $M_3$ TILLING lines have been developed via the application of gamma-ray irradiation to rice seeds (cv. Donganbyeo), followed by subsequent selections. In an effort to evaluate the genetic diversity of the TILLING population, we have employed the AFLP multiple dominant marker technique. A total of 96 (0.64%) TILLING lines as well as Donganbyeo were selected randomly and their genetic diversity was assessed based on AFLP marker polymorphisms using 5 primer combinations. An average of 100.4 loci in a range of 97 to 106 was detected using these primer combinations, yielding a total of 158 (31.4%) polymorphic loci between Donganbyeo and each of the 96 lines. A broad range of similarity from 80% to 96% with an average of 89.4% between Donganbyeo and each of the 96 lines was also observed, reflecting the genetic diversity of the TILLING population. Approximately 28 polymorphic loci have been cloned and their sequences were BLAST-searched against rice whole genome sequences, resulting in 20 matches to each of the gene bodies including exon, intron, 1 kb upstream and 1 kb downstream regions. Six polymorphic loci evidenced changes in the coding regions of genes as compared to the rice pseudomolecules, 4 loci of which exhibited missense mutations and 2 loci of which exhibited silent mutations. Therefore, the results of our study show that the TILLING rice population should prove to be a useful genetic material pool for functional genomics as well as mutation breeding applications.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.67-72
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• 2006

This experiment was carried out to identify rice varieties suitable for growing under low N-fertilization levels. Eighteen rice varieties were grown at 5.5 kg and 11 kg/10a nitrogen levels in 2004-2005 in Taegu, Korea. Several varieties like Ilmibyeo, Nampyeongbyeo, Sindongjinbyeo, Dongjin 1 and Donganbyeo yielded more than 470 kg/10a. Low N-fertilization level (5.5 kg/10a) decreased yields of Junambyeo and Donganbyeo by more than 10%. However, the decrease in yield was not significant in Surabyeo and Hwayeongbyeo compared with the 11 kg/10a N-fertilizer treatment. Improved grain quality, chemical properties and palatability was noted in some rice varieties. In terms of rice yield and grain quality, Donganbyeo, Ilmibyeo, Ilpumbyeo, Dongjin 1, Saechoocheongbyeo and Saegyehwabyeo were selected as premising rice varieties for growing under low N-fertilization levels.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.2
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• pp.107-112
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• 2013

The aim of this study was to test hypothesis that low sink size could mitigate deterioration of grain filling characteristics under high grain filling temperature. To achieve this aim, we selected Donganbyeo as a tolerant and Ilpumbyeo as susceptible variety to high temperature during grain filling period through screening 6 rice varieties. Then their spikelets number and the ratio of superior and inferior spikelet were compared. Grain weight and head rice ratio of Ilpumbyeo decreased significantly in high temperature. Ilpumbyeo had more spikelets than Donganbyeo. However, there was no significant difference between two varieties in the ratio of superior and inferior spikelets. So we tried to investigate the varietal difference of grain filling characteristics with removing inferior spikelets. Removing inferior spikelet in both of varieties could recover grain weight but not improve head rice ratio under high temperature. These results showed that sink/source ratio affected grain weight but didn't affect head rice ratio. Therefore, new approach was required to improve head rice ratio beyond sink/source ratio under high temperature.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.185-189
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• 2000

The object of this study was to determine the optimum seeding dates of "Donganbyeo", "Daesanbyeo", Hoanbyeo", "Nonghobyeo", and "Gwnganbyeo" for direct seeding on dry paddy in Youngnam region. Dry seeds of these varieties were sown 5 times with 10-day intervals from April 20 to May 30. It took 13 to 14 days from sowing to emergence. It was shortened in the delayed seeding date, but the emerged seedlings per unit area was decreased as the seeding date was advanced before May. Heading date was delayed as seeding was delayed. Culm length, panicle length and number of spikelets per panicle was not affected by seeding date, but number of panicle per $m^2$ and ripened grain ratio were high from April 30 to May 10 seeding date. Consequently, the optimum seeding date for high yield of "Donganbyeo", "Daesanbyeo", "Hoanbyeo", "Nonghobyeo", and "Gwanganbyeo" was estimated May 3, May 2, May 10, May 8, and May 1, respectively and "Gwanganbyeo" was estimated May 3, May 2, May 10, May 8, and May 1, respectively.

• Applied Biological Chemistry
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• v.46 no.1
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• pp.46-49
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• 2003

Paddy and milled rice quality, milling characteristics, energy consumption of milling process were investigated using Dasanbyeo, Ansanbyeo, Hwaseongbyeo, Ilpumbyeo, and Donganbyeo varieties. Thousand grains weights of the varieties dried to 15% moisture content were 22.92-28.60 g, with Dasanbyeo being the heaviest. Optimum clearance of rubber roller for obtaining maximum dehulling recovery was 30% of each rice variety thickness. At that time, perfect brown rice ratios of Dasanbyeo, Ansanbyeo, Hwaseongbyeo, Ilpumbyeo, and Donganbyeo were 95.24%, 98.86%, 95.97%, 97.75%, and 97.31%, respectively, and showed no significant differences among varieties. Ratios of removed rice bran after two times milling ranged 70.93-91.38%, with Dasanbyeo showing the highest ratio, and the average head rice ratio was 92.87%.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.3
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• pp.199-206
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• 2004

Typhoon "MAEMI", happened in September 2003, hit the great damage to Gyeongnam province area. Especially, rice plant was lodged or severe leaf damage was caused. This study was conducted to find out the extents of leaf damage among different rice cultivars, and to evaluate rice yield and eating quality due to leaf damage after typhoon. Rice cultivars torn off over half of the flag leaf length (FLL) were one medium-late maturing cultivar (Donginl), medium maturing cultivar (Yeonganbyeo), eight early maturing cultivars (Samcheonbyeo, Jounghwnbyeo, Munjangbyeo, Taebongbyeo, Odaebyeo, Samhaegbyeo, Sobaegbyeo, Sinunbongbyeo), two long-il type cultivars (Samgangbyeo, Namcheonbyeo), and three special use cultivars (Heukseonchalbyeo, Jinbuchalbyeo, Yangjobyeo). Cultivars torn off below 1/10 FLL were eight medium-late maturing cultivars (Chucheongbyeo, Daecheongbyeo, Saechu cheongbyeo, Donganbyeo, Daeyabyeo, Hwamyeongbyeo, 방eongsanbyeo, Dongjinbyeo) and two medium maturing cultivays (Donghaebyeo, Gumobyeo2). The rest cultivars were tore off by 1/10∼1/2 ELL. In yield components, the longer was flag leaf damage, the lower was ripened grain ratio, grain weight and brown/rough rice ratio, which was severly impacted to late than to ordinary season cultivation. However, rice yield did not decrease up to tearing by 1/10 ELL. Head rice ratio decreased from flag leaf tearing over 1/10 ELL in late season cultivation. The longer was flag leaf damage, the lower was eating quality, which could not show significantly different.

• Korean Journal Plant Pathology
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• v.13 no.2
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• pp.79-84
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• 1997

Incidence of rice blast on new rice cultivars and elite lines was observed from 194 to 1996 in Icheon, Chuncheon, Jecheon and Naju areas. The observations were made in the nuseries and in the fields. In the nurseries, only cultivars Daesanbyeo and Hyangmibyeo 2 showed moderate levels of resistance to leaf blast, with the disease index 0 to 6. From the field observations, it was found that cultivars Hyangmibyeo 2 and Suwon 414 were highly resistant to leaf blast, but susceptible to neck blast. the fields, leaf blast was not observed. In general, there was great yearly and regional variation in the incidence of neck blast within the same cultivars, some times ranging from 0 to 100% of incidence. However, the range of fluctuation in the disease incidence were relatively small in the cultivars Daejinbyeo (0∼17.5%), Daesanbyeo (0∼4.0%), Donganbyeo (0∼21.4%) and Hwasambyeo (0∼13.9%). Hyangmibyeo 2 and Seojinbyeo were rarely infected with neck blast in Chuncheon and Naju all of the years, the same cultivars were severely infested with neck blast; 45.1 and 45.5%, respectively, in Jecheon in 1995. The occurrence of different races of rice blast fungus were different at different areas. However, it was found that in Icheon, Chuncheon, Jecheon and Naju areas, the dominant races were KI-409, KJ-201 and KJ-301.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.1
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• pp.61-68
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• 2004

A reproducible transformation system via optimized regeneration media for Korean rice cultivars was established using Agrobacterium tumefeciens LBA4404 (pSBM-PPGN; gusA and bar). Although japonica rice genotypes were easier to produce transgenic plants compared to Tongil type cultivars, transformation efficiencies were not always correlated with regeneration efficiencies of non-transgenic callus on the control medium. Regeneration efficiencies of Donganbyeo, Ilmibyeo, and Manchubyeo were over 50% in non-transgenic control, however, transformation efficiencies were significantly low when only sucrose was added to the media as a carbon source. However, the medium, MSRK5SS-Pr (or MSRK5SM-Pr), that contains $5\textrm{mgL}^{-1}$ kinetin, $0.5\textrm{mgL}^{-1}$ NAA, 2 % sucrose (or maltose), 3% sorbitol, and $500\textrm{mgL}^{-1}$ proline, was the most efficient not only for regeneration of non-transgenic callus but also for regeneration of transgenic callus in the presence of L-phosphinotricin (PPT). Average transformation efficiencies of 16 Korean rice cultivars were significantly enhanced by using the optimized medium from 1.5% to 5.8% in independent callus lines and from 2.9% to 19.4% in tromsgenic plants obained. Approximately 98.9% (876 out of 885) transgenic plants obtained on optimized media showed basta resistance. Stable integration, inheritance and expression of gusA and bar genes were continued by GUS assay and PCR and Southern analysis of the bar gene. With Pst1 digestion of genomic DNA of transgenic plants, one to five copies of T-DNA segment were observed; however, 76% (19 out of 25 transgenic plants) has low copy number of T-DNA. The transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.2
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• pp.106-111
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• 1999

To investigate the effects of planting density and nitrogen level on growth and yield potential of newly bred heavy panicle japonica rice with large grain (Iksan 435 and Iksan 438) or many spikelets per panicle(HR14022-21-8-4 and HR14022-21-8-6), four heavy panicle type rices and two many panicle type rices(Dongjinbyeo and Donganbyeo) as the checks were planted under standard planting density (30$\times$15 cm) and dense planting density (15$\times$15 cm) with two nitrogen levels of standard nitrogen level(110 kg h $a^{-1}$) and heavy nitrogen level(165 kg h $a^{-1}$). Effective tiller rate decreased in dense planting or heavy nitrogen, when compared to standard nitrogen and planting, while leaf area index and to dry weight increased in dense planting or heavy nitrogen. Tiller numbers and panicle numbers were more increased by dense planting than heavy nitrogen, whereas spikelet numbers were more increased by heavy nitrogen than dense planting. Ripened grain ratio was slightly lower only in dense planting. 1,000 grain weight in brown rice was not significantly different in dense planting or heavy nitrogen. Milled rice yield was highest in heavy nitrogen with standard planting for heavy panicle type rice, while yield for many panicle type rice was highest in heavy nitrogen with dense planting, suggesting that many panicle type rice possesses higher adapt-ability for dense planting than heavy panicle type rice. Path coefficient analysis revealed that top dry weight, spikelet number and grain weight were the greatest positive contributors to yield, whereas tiller number was negative to yield.d.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.61 no.4
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• pp.235-241
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• 2016

Three different transplanting dates for two rice cultivars grown in Daegu, Republic of Korea, were examined to identify the changes in growth, rice quality, and characteristics of amylopectin. An early transplanting date caused a reduction in the number of panicles in both rice cultivars compared to that in the optimal and late transplanting dates. The 1000-grain weight in the two rice cultivars was significantly increased in the late transplanting date. The rice cultivar tolerant to high temperatures, Donganbyeo, exhibited the lowest milky grain rate in the late transplanting date. The highest rate of head grain was observed in the late transplanting date in both rice cultivars. Regarding the pattern of pasting properties, peak viscosity increased with delayed transplanting dates. With respect to changes in the amylopectin branch-chain length distribution, the amylopectin structure of the translucent Dongan rice cultivar transplanted on April 25 was characterized by a significant increase in A chains with DP > 12, and a decrease in long chains $DP{\geq}37$ compared to that transplanted on June 25. In contrast, the amylopectin structure of the chalky Dongan rice cultivar transplanted on April 25 exhibited further decrease in 13 < DP < 19 than that transplanted on June 25. In the Ungwang rice cultivar, the amylopectin structure of the translucent Ungwang rice cultivar transplanted on April 25 and June 25 was characterized by a significant decrease in the lengths of total amylopectin chains. Furthermore, the amylopectin structure of the chalky Ungwangbyeo rice cultivar transplanted on April 25 was characterized by a significant increase in 7 < DP < 30, while a significant decrease was observed in that transplanted on June 25. These results indicate that the amylopectin structure is altered by different transplanting dates depending on the characteristics of the rice cultivar.