• Korean Journal of Veterinary Service
• /
• v.38 no.1
• /
• pp.43-49
• /
• 2015

Bovine viral diarrhea virus (BVDV) is a very important viral disease virus in cattle, domestic and wild ruminants. The purpose of this study is to investigate the positive rate of bovine viral diarrhea virus antigen by ELISA from Korean native and beef cattle reared in Busan area from March in 2013 to October in 2014. A total of 1,129 bovine blood samples were collected from 140 farms, 1,111 Korean native cattle of 135 farms and 18 beef cattle of 5 farms. Test for antigen was carried out by ELISA method. In general analysis, the positive rate of bovine viral diarrhea virus antigen were 0.7% (8/1,129) cattle and 5.0% (7/140) farm. In regional analysis, the positive rate of BVDV antigen of farm in Kijang-gun, Gangseo-gu, Geumjeong-gu, Saha-gu and Dongnae-gu were 1.4% (2/94), 3.6% (5/37), 0% (0/7), 0% (0/1) and 0% (0/1), respectively, and the positive rate of BVDV antigen of cattle were 0.4% (3/770), 1.5% (5/333), 0% (0/24), 0% (0/1) and 0% (0/1), respectively. The positive rate of BVDV antigen according to sex were 0.6% (6/1,085) female cattle and 4.6% (2/44) male cattle. According to the age of cattle, the positive rate of BVDV antigen in 1 year, 2 years, 3 years and 5 years old were 1.9% (4/215), 0.4% (1/265), 0.9% (2/234) and 1.0% (1/103), respectively, but 4 years (0/198), 6 years (0/55), 7 years (0/24), 8 years (0/14), 9 years (0/10), 10 years (0/7) and 11-15 years (0/3) old were negative, respectively.

• Asian-Australasian Journal of Animal Sciences
• /
• v.27 no.10
• /
• pp.1381-1386
• /
• 2014

In spite of variation in coat color, size, and production traits among indigenous Bangladeshi cattle populations, genetic differences among most of the populations have not been investigated or exploited. In this study, we used a high-density bovine single nucleotide polymorphism (SNP) 80K Bead Chip derived from Bos indicus breeds to assess genetic diversity and population structure of 2 Bangladeshi zebu cattle populations (red Chittagong, n = 28 and non-descript deshi, n = 28) and a semi-domesticated population (gayal, n = 17). Overall, 95% and 58% of the total SNPs (69,804) showed polymorphisms in the zebu and gayal populations, respectively. Similarly, the average minor allele frequency value was as high 0.29 in zebu and as low as 0.09 in gayal. The mean expected heterozygosity varied from $0.42{\pm}0.14$ in zebu to $0.148{\pm}0.14$ in gayal with significant heterozygosity deficiency of 0.06 ($F_{IS}$) in the latter. Coancestry estimations revealed that the two zebu populations are weakly differentiated, with over 99% of the total genetic variation retained within populations and less than 1% accounted for between populations. Conversely, strong genetic differentiation ($F_{ST}=0.33$) was observed between zebu and gayal populations. Results of population structure and principal component analyses suggest that gayal is distinct from Bos indicus and that the two zebu populations were weakly structured. This study provides basic information about the genetic diversity and structure of Bangladeshi cattle and the semi-domesticated gayal population that can be used for future appraisal of breed utilization and management strategies.

• Parasites, Hosts and Diseases
• /
• v.44 no.1 s.137
• /
• pp.27-33
• /
• 2006

Two species of Cryptosporidium are known to infect man; C. hominis which shows anthroponotic transmission between humans, and C. parvum which shows zoonotic transmission between animals or between animals and man. In this study, we focused on identifying genotypes of Cryptosporidium prevalent among inhabitants and domestic animals (cattle and goats), to elucidate transmittal routes in a known endemic area in Hwasun-gun, Jeollanam-do, Republic of Korea. The existence of Cryptosporidium oocysts was confirmed using a modified ZiehlNeelsen stain. Human infections were found in 7 $(25.9\%)$ of 27 people examined. Cattle cryptosporidiosis cases constituted 7 $(41.2\%)$ of 17 examined, and goat cases 3 $(42.9\%)$ of 7 examined. Species characterizations were performed on the small subunit of the rRNA gene using both PCR-RFLP and sequence analysis. Most of the human isolates were mixtures of C. hominis and C. parvum genotypes and similar PCR-RFLP patterns were observed in cattle and goat isolates. However, sequence analyses identified only C. hominis in all isolates examined. The natural infection of cattle and goats with C. hominis is a new and unique finding in the present study. It is suggested that human cryptosporidiosis in the studied area is caused by mixtures of C. hominis and C. parvum oocysts originating from both inhabitants and domestic animals.

• Korean Journal of Veterinary Service
• /
• v.26 no.1
• /
• pp.11-18
• /
• 2003

A total of 1,024 sera were collected from cattle(227), pigs(465), chickens(257) and dogs(75) raised or slaughtered in Daejeon metropolitan city from April to September 2002. Japanses encephalitis virus(JEV) antibodies in sera were detected by the haemagglutination inhibition test. The prevalence rates of JEV antibodies were 99.1 %, 54.0 %, 63.0 % and 98.7 % in cattle, pigs, chickens and dogs, respectively. In case of cattle and dogs, the monthly antibody-positive rates were as high as 85.7∼100.0 % and there were no differences among six months. In case of pigs, the monthly antibody-positive rate showed the lowest in April(6.4 %) and the highest in July(100.0 %) and it remained above 50 % during the summer-time. In case of chickens, the monthly antibody-positive rate was 100.0 % in July & August, 80.5 % in June, 40.0 % in May, 7.5 % in September and 5.0 % in April in order and there were distinct differences in seasons.

• Parasites, Hosts and Diseases
• /
• v.45 no.4
• /
• pp.267-271
• /
• 2007

Theileria annulata, a protozoan parasite of cattle and domestic buffaloes, is transmitted by ticks of the genus Hyalomma, and causes a disease named Mediterranean or tropical theileriosis. In this research 50 cattle naturally infected with Theileria annulata were treated with the extract of the plant Peganum harmala. The treatment was continued for 5 days, the dose of the extract being 5 mg/kg per day. After the treatment, 39 cattle responded to the treatment and recovered, but 11 did not respond to the treatment and died. The recovery rate of animals treated with the extract of the plant Peganum harmala was 78%.

• Journal of Veterinary Clinics
• /
• v.32 no.6
• /
• pp.504-507
• /
• 2015

Coxiella burnetii is an obligate intracellular rickettsial organism and the causative agent of Query fever, a zoonosis that occurs worldwide. In Korea, C. burnetii infection had occurred in humans and animals. However, the studies were only conducted in geographically limited area for detection of C. burnetii. The objective of this study was to detect C. burnetii in Korean native cattle and dairy cattle nationwide by real-time PCR. The total of 807 blood samples from 622 Korean native cattle and 185 dairy cows, 170 individual milk samples of dairy cows, and 348 bulk tank milk samples of dairy herds were collected nationwide. From blood samples, C. burnetii was detected in 17 (2.7%) out of 622 Korean native cattle and 2 (1.1%) of 185 dairy cows. From milk samples, C. burnetii was detected in 27 (15.9%) out of 170 individual milk samples of dairy cows. And C. burnetii was detected in 84 (24.1%) of 348 bulk tank milk samples. In conclusion, this study revealed that the detection rates are considerably high in cattle and the infection of C. burnetii has been continuously occurring in cattle of Korea. In order to prevent the hazards of a zoonosis Q-fever that occur both humans and domestic animals, further studies are needed to clarify the epidemiology of Q-fever of domestic animals and humans in Korea.

• Korean Journal of Veterinary Research
• /
• v.26 no.2
• /
• pp.211-219
• /
• 1986

In order to know the comparative histological structure of the tongue of some domestic and laboratory animals, the root of the tongue has been investigated by light microscopy. The filiform papillae were mainly distributed in the root of the tongue of most animals and many foliate papillae were also found in the dog and rabbit. On the other hand, only filiform papillae were found in the fowl. The very well-developed mucosa was found in the cattle and in the dog, fowl, rat and mouse was fair. The lamina propria was well developed in the cattle, dog and pig, The muscle layer was well developed in the cattle. The adipose tissue which distributed between the muscle fibers was mainly found in the dog. The lingual glands were well developed in the pig, fowl and rabbit. The properties of these glands were shown strung acid mucopolysaccharide in the dog and pig, weak acid mucopolysaccharide in the fowl, rabbit and rat and neutral or acid mucopolysaccharide in the mouse. The species differences on the histological structure of the tongue were also ascertained by light microscopical observations.

• Korean Journal of Veterinary Research
• /
• v.38 no.2
• /
• pp.353-358
• /
• 1998

A mysterious disease with vesicle-like nodules in dairy cattle udder has been drawn attention to the foreign animal disease expert at the National Veterinary Research Institute. Immediately dispatched foreign animal disease expert, pathologist and regional veterinary officials executed quarantine nearby affected farm area to prevent transmission of the pathogen which was possibly contagious vesicular disease agents in domestic animals such as FMD or SVD. Proper samples were collected for the laboratory examination. Vesicle-like lesions were only detected in lactating dairy cattle and no distinct clinical signs have been observed in affected animals. Parapox and papillomavirus particles have been demonstrated on electromicroscopical examination from nodular samples of udder lesions of the dairy cattle. Characteristic papilloma virus particles with 55nm in diameter and parapoxvirus with 150nm in diameter and 350nm long oval shape particles were detected and confirmed by embryonated chicken egg inoculation.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.9
• /
• pp.1238-1243
• /
• 2008

Domestic buffalo and cattle are two extremely important livestock species in worldwide agricultural production. In this paper, to investigate genetic diversity and divergence among swamp buffalo, river buffalo and cattle, 30 microsatellite markers were screened on 168 individuals sampled from five populations. Substantial differences were observed among the three groups of animals with respect to allele frequency distribution, allele size and polymorphism. The cattle sample (Mongolian) showed significantly higher genetic variability (0.674 of gene diversity, p<0.01), and the swamp and river buffalo samples displayed similar degree of genetic variation (0.536 in swamp and 0.546 in river, p = 0.92). Results of both phylogenetic tree and multivariate analysis could distinguish three groups of animals, suggesting their deep evolutionary divergence. Additionally, using $({\delta}{\mu})^2$ genetic distance, we estimated a divergence time of 1.7 million years between swamp and river buffalo that strongly supported distinct genetic origins for the two buffalo types.

• Food Science of Animal Resources
• /
• v.31 no.3
• /
• pp.405-412
• /
• 2011

The purpose of this study was to isolate and characterize bacteriocin-producing bacteria against Clostridium perfringens from domestic animals to determine their usefulness as probiotics. The feces of cattle and chicken were used as sources to isolate bacteriocin-producing bacteria using the spot-on-lawn method. In total, 900 bacterial stains were isolated from domestic animal feces, and 19 strains were finally selected after determining the inhibitory activity against the pathogenic indicator C. perfringens KCTC 3269. Eighteen strains of Bacillus subtilis and one strain of Brevibacillus parabrevis were identified by 16s rRNA sequencing. Most of the bacterial strains isolated were resistant to 0.5% bile salts and remained viable after 2 h at pH 3.0. Additionally, some B. subtilis strains showed strong inhibitory activity against Listeria monocytogenes. We isolated and screened B. subtilis strains CB 153 and CB 189 from cattle and B. subtilis MSC 156 and B. parabrevis MSC 164 from chickens using probiotic selection criteria such as inhibition activity against C. perfringens and tolerance to acid and bile salts. The isolated bacteriocin-producing bacteria and/or bacteriocin have the potential to be used as probiotics in the livestock industry.