• 한국정보시스템학회지:정보시스템연구
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• 제16권4호
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• pp.131-155
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• 2007

The purpose of this thesis is to study on the philosophical analysis model and its methodological application of information systems research evaluation from critical realist perspective. Fist of all, I examine ontological epistemological methodological assertions of critical realism. Because the philosophy of critical realism is an opportunity for information systems study. I examine Dobson and Mutch's critical realist perspective on actors-structure model. I suggest a critical realist actors-praxis-structure model. This model provides the potential for a new approach to social investigations in its provision of an ontology for the analytical separation of structure and agency. Of most importance might be the incorporation of non-humans into the analysis of social interaction and of technology into the elaboration of structures. I also examine Tsoukas's critical realistic meta-theory of management. I suggest a critical realist IS management model. This model elucidate the nature of management and delineate the scope of applicability of various perspectives on management. The causal powers of management reside in the real domain and, taken together, their logics are contradictory, the effects of their contradictory composition are contingent upon prevailing contingencies. I analyze Carlsson's theory of design knowledge. His framework builds on that the aim of IS design science research is to develop practical knowledge for the design and realization of different classes of IS initiatives, where IS are viewed as socio-technical systems and not just IT artefacts. The framework proposes that the output of IS design science research is practical IS design knowledge in the form of field-tested and grounded technological rules. The IS design knowledge is developed through an IS design science research cycle. In conclusion, I think that IS actors-praxis-structure model, meta-theoretical IS management model, and IS design knowledge model according to critical realistic approach are very useful for IS research evaluation. Nevertheless, important problems are left not resolved.

• Journal of Acupuncture Research
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• 제22권2호
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• pp.123-132
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• 2005

Melittin,cationic 26-amino acid, is the principal component of the bee venom (BV) which has been used for treatment of inflammatory disease such as arthritis rheumatism NF-kB is activated by subsequent release of inhibitory IkB via activation of a multisubunit IkB kinase (IKK). We previously found that melittin bind to the sulfhydryl group of p50, a subunit of NF-kB. Since sulfhydryl group is present in kinase domain of IKKa and IKKb, melittin could modify IKK activity by protein-protein interaction. We therefore examined effect of melittin on IKK activities in sodium nitroprusside (SNP)-stimulated synoviocyte obtained from RA patients. Melittin suppressed the SNP-induced release of IkB resulted in inhibition of DNA binding activity of NF-kB and NF-kB-dependent luciferase activity. Consistent with the inhibitory effect on NF-kB activation, IKKa and IKKb activities were also suppressed by melittin. Surface plasmon resonance analysis realized that melitin binds to IKKa $(Kd\;=\;1.34{\times}10-9M)$ and IKKb$(Kd\;=\;1.0{\times}10-9M)$. Inhibition of IKKa and IKKb resulted in reduction of the SNP-induced production of inflammatory mediators NO and PGE2 generation. The inhibitory effect of melittin on the IKKs activities, binding affinity of melittin to IKKs, and NO and PGE2 generation were blocked by addition of reducing agents dithiothreitol and glutathione. In addition, melittin did not show inhibitory effect in the transfected Synoviocytes with plasmid carrying dominant negative mutant IKKa (C178A) and IKKb (C179A). These results demonstrate that melittin directly binds to sulfhydryl group of IKKs resulting in IkBrelease, thereby inhibits activation of NF-kB and expression of genes involving in the inflammatory responses.

• 대한조선학회지
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• 제23권4호
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• pp.25-34
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• 1986

A two-dimensional linear method has been developed for the motion and the second-order steady force arising from the hydrodynamic coupling between waves and currents in the presence of a body of arbitrary shape. Interaction between the incident wave and current in the absence of the body lies in the realm beyond our interest. A Fredholm integral equation of the second kind is employed in association with the Haskind's potential for a steadily moving source of pulsating strength located in or below the free surface. The numerical calculations at the preliminary stage showed a significant fluctuation of the hydrodynamic forces on the surface-piercing body. The problem is approximately solved by using the asymptotic Green function for $U^2{\rightarrow}0$. The original Green function, however, is applied for the fully submerged body. Numerical calculations are made for a submerged and for a half-immersed circular cylinder and extensively for the mid-ship section of a Lewis-form. Some of the results are compared with other analytical results without any available experimental data. The current has strong influence on roll motion near resonance. When the current opposes the waves, the roll response are generally negligible in the low frequency region. The current has strong influence on roll motion near resonance. When the current opposes the wave, the roll response decreases. When the current and wave come from the same direction, the roll response increases significantly, as the current speed increases. The mean drift forces and moment on the submerged body are more affected by current than those on the semi-immersed circular cylinder or on the ship-like section in the encounter frequency domain.

• Bulletin of the Korean Chemical Society
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• 제33권6호
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• pp.1839-1844
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• 2012

We have investigated binding between wild-type and mutant Heat Shock Factor (HSF) DNA binding domains (DBDs) with 17-bp HSE containing a central 5'-NGAAN-3' element by equilibrium analytical ultracentrifugation using multi-wavelength technique. Our results indicate that R102 plays critical role in HSE recognition and the interactions are characterized by substantial negative changes of enthalpy (${\Delta}H^0_{\theta}=-9.90{\pm}1.13kcal\;mol^{-1}$) and entropy (${\Delta}S^0_{\theta}=-12.46{\pm}3.77cal\;mol^{-1}K^{-1}$) with free energy change, ${\Delta}G^0_{\theta}$ of $-6.15{\pm}0.03kcal\;mol^{-1}$. N105 plays minor role in the HSE interactions with ${\Delta}H^0_{\theta}$ of $-2.54{\pm}1.65kcal\;mol^{-1}$, ${\Delta}S^0_{\theta}$ of $19.28{\pm}5.50cal\;mol^{-1}K^{-1}$ and ${\Delta}G^0_{\theta}$ of $-8.35{\pm}0.05kcal\;mol^{-1}$, which are similar to those observed for wild-type DBD:HSE interactions (${\Delta}H^0_{\theta}=-3.31{\pm}1.86kcal\;mol^{-1}$, ${\Delta}S^0_{\theta}=17.38{\pm}6.20cal\;mol^{-1}K^{-1}$ and ${\Delta}G^0_{\theta}=-8.55{\pm}0.06kcal\;mol^{-1}$) indicating higher entropy contribution for both wild-type and N105A DBD bindings to the HSE.

• Structural Engineering and Mechanics
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• 제12권6호
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• pp.657-668
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• 2001

Fiber reinforced cementitious composites are nowadays widely applied in civil engineering. The postcracking performance of this material depends on the interaction between a steel fiber, which is obliquely across a crack, and its surrounding matrix. While the partly debonded steel fiber is subjected to pulling out from the matrix and simultaneously subjected to transverse force, it may be modelled as a Bernoulli-Euler beam partly supported on an elastic foundation with non-linearly varying modulus. The fiber bridging the crack may be cut into two parts to simplify the problem (Leung and Li 1992). To obtain the transverse displacement at the cut end of the fiber (Fig. 1), it is convenient to directly solve the corresponding differential equation. At the first glance, it is a classical beam on foundation problem. However, the differential equation is not analytically solvable due to the non-linear distribution of the foundation stiffness. Moreover, since the second order deformation effect is included, the boundary conditions become complex and hence conventional numerical tools such as the spline or difference methods may not be sufficient. In this study, moment equilibrium is the basis for formulation of the fundamental differential equation for the beam (Timoshenko 1956). For the cantilever part of the beam, direct integration is performed. For the non-linearly supported part, a transformation is carried out to reduce the higher order differential equation into one order simultaneous equations. The Runge-Kutta technique is employed for the solution within the boundary domain. Finally, multi-dimensional optimization approaches are carefully tested and applied to find the boundary values that are of interest. The numerical solution procedure is demonstrated to be stable and convergent.

• Membrane and Water Treatment
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• 제7권3호
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• pp.223-240
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• 2016

In this study, poly(vinyl-alcohol) and water insoluble ${\beta}$-cyclodextrin polymer (${\beta}$-CDP) cross-linked with citric acid, have been used as macrocyclic carrier in the preparation of polymer inclusion membranes (PIMs) for aniline (as molecule model) extraction from aqueous media. The obtained membranes were firstly characterized by X-ray diffraction, Fourier transform infrared and water swelling test. The transport of aniline was studied in a two-compartment transport cell under various experimental conditions, such as carrier content in the membranes, stirring rate and initial aniline concentration. The kinetic study was performed and the kinetic parameters were calculated as rate constant (k), permeability coefficient (P) and flux (J). These first results demonstrated the utility of such polymeric membranes for environmental decontamination of toxic organic molecules like aniline. Predictive modeling of transport flux through these materials was then studied using design of experiments; the design chosen was a two level full factorial design $2^k$. An empirical correlation between aniline transport flux and independent variables (Poly ${\beta}$-CD membrane content, agitation speed and initial aniline concentration) was successfully obtained. Statistical analysis showed that initial aniline concentration of the solution was the most important parameter in the study domain. The model revealed the existence of a strong interaction between the Poly ${\beta}$-CD membrane content and the stirring speed of the source solution. The good agreement between the model and the experimental transport data confirms the model's validity.

• 한국자기학회지
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• 제8권5호
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• pp.249-254
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• 1998

자기측정법에 의한 조사손상 평가 가능성을 조사하기 위하여 SA508 CI.3강 모재금속의 중성자 조사 및 열처리 온도에 따른 자기특성(자기이력곡선, Barkhausen Noise(BN) 진폭, BN 에너지)과 경도 변화를 측정 비교하였다. 중성자 조사에 따라 자화율, BN 진폭, BN 에너지는 감소하였고 보자력과 경도는 증가하였으며, 포화자화 값은 변화하지 않았다. 열처리된 조사시편의 경우, 열처리 온도 증가에 따라 BN 에너지는 증가하였으며, 경도는 감소하였다. 결함과 전위혹은 자벽 이동과의 상호작용에 의한 경도 및 자기특성의 일관성 있는 변화는 원자로 압력용기 재료의 조사손상 평가와 관련 자기적 측정법 응용 가능성을 보여주었다.

• 한국산학기술학회논문지
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• 제9권6호
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• pp.1795-1799
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• 2008

hnRNP K 단백질은 hnRNP 복합체를 구성하는 핵단백질들 중의 하나이며 시토신이 많은 RNA/DNA sequence에 잘 결합한다. 이 단백질은 핵 내에서만 머무르지 않고 핵과 세포질을 왕복하는 특징을 지니고 있다. hnRNP K의 기능을 조사하기 위하여 우선 hnRNP K와 상호 결합하는 세포내 단백질을 찾아내고자 하였다. 이를 위하여 본 연구에서는 이스트 two-hybrid 시스템을 사용하여 HeLa CDNA librar를 탐색하였다. 그 결과 얻은 클론들 중에는 사람의 hnRNP E1 (poly(rC) binding protein 1) cDNA (GenBank accession number XM_031585) 클론이 포함되어 있었다. 본 논문에서는 이스트 two-hybrid 시스템과 in vitro에서의 생화학적 실험을 통하여 hnRNP E1은 hnRNP K와 특이적으로 상호 결합한다는 것을 밝혔다.

• Genomics & Informatics
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• 제13권2호
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• pp.53-59
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• 2015

In developing countries threat of cholera is a significant health concern whenever water purification and sewage disposal systems are inadequate. Vibrio cholerae is one of the responsible bacteria involved in cholera disease. The complete genome sequence of V. cholerae deciphers the presence of various genes and hypothetical proteins whose function are not yet understood. Hence analyzing and annotating the structure and function of hypothetical proteins is important for understanding the V. cholerae. V. cholerae O139 is the most common and pathogenic bacterial strain among various V. cholerae strains. In this study sequence of six hypothetical proteins of V. cholerae O139 has been annotated from NCBI. Various computational tools and databases have been used to determine domain family, protein-protein interaction, solubility of protein, ligand binding sites etc. The three dimensional structure of two proteins were modeled and their ligand binding sites were identified. We have found domains and families of only one protein. The analysis revealed that these proteins might have antibiotic resistance activity, DNA breaking-rejoining activity, integrase enzyme activity, restriction endonuclease, etc. Structural prediction of these proteins and detection of binding sites from this study would indicate a potential target aiding docking studies for therapeutic designing against cholera.

• 한국해안·해양공학회논문집
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• 제22권6호
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• pp.423-428
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• 2010

본 계류안정성 해석은 광양만 부두의 포항제철 제품 및 고철부두에 대한 적정 선박을 선정하는 방법의 하나로서 수행되었다. 계류안정성 해석을 위해서는 우선 제품부두에 정박하는 기준이 되는 대상선박을 선정하여 각 선박의 운동특성을 파악하고, 이것을 바탕으로 각 대상 선박의 유체력을 계산한 후 파랑, 바람 및 조류를 고려하여 안벽에 계류된 선박의 계류안정성해석을 수행하였다. 이를 통해 광양 제품부두에서의 계류안정성을 보장하는 적정한 선박 선정기준을 개선하고자 하였다.