• The Journal of the Korea Contents Association
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• v.17 no.2
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• pp.288-297
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• 2017

Online education is a new domain of learning that combines distance learning with the practice of face-to-face instruction utilizing computer-mediated communication. As technology continues to develop and the needs of teachers require better access to higher learning, online graduate degree programs can be a valid alternative. Some institutions in the United States have already offered graduate degrees in music education through the online distance learning. In this study, 8 accredited online graduate degree programs in music education were identified in terms of curriculum requirement, program requirement, and admission requirement. Online programs offer considerable benefits of convenience, while their drawbacks relates to the quality of learning, feelings of weaker interpersonal interaction. More researches need in order for online music programmers to achieve higher standards of instruction and to inform other disciplines in the fields of arts education and the performing arts.

• Journal of the Korean Society for Marine Environment & Energy
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• v.13 no.2
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• pp.83-90
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• 2010

The hydrodynamic interaction of two floating bodies in waves freely floating or connected by a rigid link is studied by using a boundary element method in the frequency-domain. The exact two-body hydrodynamic coefficients of added mass, wave damping and exciting force are calculated from the radiation-diffraction potential solution of the improved Green integral equation associated with the free surface Green function. The irregular frequencies in the conventional Green integral equation make it difficult to predict the physical resonance of the fluid in the gap between two bodies floating side by side. However, the improved Green integral equation employed in this study is free of irregular frequencies and always yields the exact solution of the multi-body radiation-diffraction potential boundary value problem. The 6 degree-of-freedom motions of two bodies freely floating side by side or connected parallel by a rigid link have been calculated for the incident wave frequencies ranging from 0.1 to 5 radians per second in head, left and right bow quartering seas. The 6-component load of the rigid link have also been presented.

• The Korean Journal of Physiology and Pharmacology
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• v.12 no.4
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• pp.211-216
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• 2008

TREK (TWIK-RElated $K^+$ channels) and TRAAK (TWIK-Related Arachidonic acid Activated $K^+$ channels) were expressed in COS-7 cells, and the channel activities were recorded from inside-out membrane patches using holding potential of - 40 mV in symmetrical 150 mM $K^+$ solution. Intracellular application of an oxidizing agent, 5,5'-dithio-bis (2-nitrobenzoic acid) (DTNB), markedly decreased the activity of the TREK2, and the activity was partially reversed by the reducing agent, dithiothreitol (DTT). In order to examine the possibility that the target sites for the oxidizing agents might be located in the C-terminus of TREK2, two chimeras were constructed: TREK2 (1-383)/TASK3C and TREK2 (1-353)/TASK3C. The channel activity in the TREK2 (1-383)/TASK3C chimera was still inhibited by DTNB, but not in the TREK2 (1-353)/TASK3C chimera. These results indicate that TREK2 is inhibited by oxidation, and that the target site for oxidation is located between the amino acid residues 353 and 383 in the C-terminus of the TREK2 protein.

• Journal of Microbiology and Biotechnology
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• v.30 no.10
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• pp.1488-1494
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• 2020

Rho guanine nucleotide dissociation inhibitor 1 (RhoGDI1) plays important roles in numerous cellular processes, including cell motility, adhesion, and proliferation, by regulating the activity of Rho GTPases. Its expression is altered in various human cancers and is associated with malignant progression. Here, we show that RhoGDI1 interacts with Cullin 3 (CUL3), a scaffold protein for E3 ubiquitin ligase complexes. Ectopic expression of CUL3 increases the ubiquitination of RhoGDI1. Furthermore, potassium channel tetramerization domain containing 5 (KCTD5) also binds to RhoGDI1 and increases its interaction with CUL3. Ectopic expression of KCTD5 increases the ubiquitination of RhoGDI1, whereas its knockdown by RNA interference has the opposite effect. Depletion of KCTD5 or expression of dominant-negative CUL3 (DN-CUL3) enhances the stability of RhoGDI1. Our findings reveal a previously unknown mechanism for controlling RhoGDI1 degradation that involves a CUL3/KCTD5 ubiquitin ligase complex.

• Journal of Veterinary Clinics
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• v.21 no.3
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• pp.253-258
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• 2004

DNA double-strand break (DSB) is a serious treat for the cells including mutations, chromosome rearrangements, and even cell death if not repaired or misrepaired. Ku heterodimer regulatory DNA binding subunits (Ku70/Ku80) bound to double strand DNA breaks are able to interact with 470-kDa DNA-dependent protein kinase catalytic subunit (DNA-PKcs), and the interaction is essential for DNA-dependent protein kinase (DNA-PK) activity. The Ku80 mutants were designed to bind Ku70 but not DNA end binding activity and the peptides were treated in breast cancer cells for co-therapy strategy to see whether the targeted inhibition of DNA-dependent protein kinase (DNA-PK) activity sensitized breast cancer cells to ionizing irradiation or chemotherapy drug to develop a treatment of breast tumors by targeting proteins involved in damage-signaling pathway and/or DNA repair. We designed domains of Ku80 mutants, 26 residues of amino acids (HN-26) as a control peptide or 38 (HNI-38) residues of amino acids which contain domains of the membrane-translocation hydrophobic signal sequence and the nuclear localization sequence, but HNI-38 has additional twelve residues of peptide inhibitor region. We observed that the synthesized peptide (HNI-38) prevented DNA-PKcs from binding to Ku70/Ku80, resulting in inactivation of DNA-PK complex activity in breast cancer cells (MDA-465 and MDA-468). Consequently, the peptide treated cells exhibited poor to no DNA repair, and became highly sensitive to irradiation or chemotherapy drugs. The growth of breast cancer cells was also inhibited. These results demonstrate the possibility of synthetic peptide to apply breast cancer therapy to induce apoptosis of cancer cells.

• Journal of the Korea Convergence Society
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• v.8 no.5
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• pp.201-211
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• 2017

Social scientists have studied interaction between human beings, while computer scientists have expanded the research domain from human-human to human-machine, human-agent, or machine-machine. The reason why an adoption of Smart Work is failed is an anxiety about ICT usage which middle managers have. It is important to explore the concept both to reduce an anxiety on an application and to increase continuance to use it. Therefore this study takes "empathy" as a key factor to play a leading role both to relieve the anxiety about the application and to improve the intention to use it. The data is gathered from a survey of undergraduate who have experience to use MS-Access. The findings show that application empathy decrease the application anxiety, but the empathy increase the continuance mediated by cognitive and affective attitude.

• Biomedical Science Letters
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• v.13 no.1
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• pp.1-10
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• 2007

Nebulin, a giant modular protein from muscle, is thought to act as molecular ruler in sarcomere assembly. In skeletal muscle, the C-terminal ${\sim}50 kDa$ region of nebulin extends into the Z-line lattice. The most recent studies implicated highlighting its extensive isoform diversity and exciting reports revealed its expression in cardiac and non-muscle tissues containing brain. Also these novel findings are indicating that nebulin is actually a multifunctional filament system, perhaps playing roles in signal transduction, contractile regulation, and myofibril force generation, as well as other not yet defined functions. However the binding protein of nebulin and function in brain is still unknown. A novel binding partner of nebulin C-terminal region was identified by screening a human brain cDNA library using yeast two-hybrid system. Nebulin C-terminus binding protein 51 (NCBP51) was contained a RING-finger domain and identified a new isoform of RING finger protein 125 (RNF125). The interaction was confirmed using the GST pull-down assay. NCBP51 belongs to a family of the RING finger proteins and its function remains to be identified in brain. The role of nebulin and NCBP51 will be studied by loss-of-function using siRNA technique in brain.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.5
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• pp.1033-1042
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• 1994

Dynamic behavior of flexible rectangular liquid containers is analyzed by a two-dimensional coupled boundary element-finite element method. The irrotational motion of inviscid and incompressible ideal fluid is modeled by boundary elements and the motion of structure by finite elements. A singularity free integral formulation is employed for the implementation of boundary element method. Coupling is performed by using compatibility and equilibrium conditions along the interface between the fluid and structure. The fluid-structure interaction effects are reflected into the coupled equation of motion as added fluid mass matrix and sloshing stiffness matrix. By solving the eigen-problem for the coupled equation of motion, natural frequencies and mode shapes of coupled system are obtained. The free surface sloshing motion and hydrodynamic pressure developed in a flexible rectangular container due to horizontal and vertical ground motions are computed in time domain.

• Herbal Formula Science
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• v.14 no.2
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• pp.1-8
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• 2006

ElM (Eighteen Incompatible Medicaments) is an important component in Oriental pharmacology and is directly related to clinical prescriptions. Medical practitioners argued that the definite cause and meaning of ElM was ambiguous and therefore debated the issue of clinical application of the ElM. This study conducted an in-depth literary research on the origin, meaning and contents of ElM with the purpose to contribute in its efforts to be used clinically. Even after thousands of years have past since establishment of Oriental medicine. ElM is still tabooed and was an obstacle that hindered ideologies. Modern herbal medicine texts claim that the use of ElM can reduce treatment effects and promote poisoning and side effects. However, since long ago, there has been medical practitioners who reject this as false. Recently, poisoning caused by ElM has been claimed to be from the toxicity of the drug itself, rather than the result of interaction between the drugs. and therefore they suggest that ElM is not a forbidden domain. In addition. ElM showed a difference in number depending on the era. However, this can be understood not as a definite number. but instead as a warning to be careful during combination of drugs for use as clinical medicine. Historically. there were very few cases in which ElM was used for clinical tests and thus, the clinical value is not yet clear. However. some medical practitioners clinically applied it and found clear results for it. while others applied ElM directly to their bodies. which showed signs for the usefulness and potential of ElM for us. A more concrete and in-depth study must be made on ELM.

• BMB Reports
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• v.39 no.5
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• pp.530-536
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• 2006

Thermal conformational changes of human serum albumin (HSA) in phosphate buffer, 10 mM at pH = 7 are investigated using differential scanning calorimetric (DSC), circular dichroism (CD) and UV spectroscopic methods. The results indicate that temperature increment from $25^{\circ}C$ to $55^{\circ}C$ induces reversible conformational changes in the structure of HSA. Conformational change of HSA are shown to be a three-step process. Interestingly, melting temperature of the last domain is equal to the maximum value of fever in pathological conditions, i.e. $42^{\circ}C$. These conformational alterations are accompanied by a mild alteration of secondary structures. Study of HSA-SDS (sodium dodecyl sulphate) interaction at $45^{\circ}C$ and $35^{\circ}C$ reveals that SDS affects the HSA structure at least in three steps: the first two steps result in more stabilization and compactness of HSA structure, while the last one induces the unfolding of HSA. Since HSA has a more affinity for SDS at $45^{\circ}C$ compared to $35^{\circ}C$, It is suggested that the net negative charge of HSA is decreased in fever, which results in the decrease of HSA-associated cations and plasma osmolarity, and consequently, heat removal via the increase in urine volume.